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The map label for this gene is livJ [H]

Identifier: 55980559

GI number: 55980559

Start: 556791

End: 557975

Strand: Reverse

Name: livJ [H]

Synonym: TTHA0590

Alternate gene names: 55980559

Gene position: 557975-556791 (Counterclockwise)

Preceding gene: 55980560

Following gene: 55980558

Centisome position: 30.17

GC content: 68.61

Gene sequence:

>1185_bases
ATGAAGCGCAGGGAGTTTCTGAGGAGGCTTGGCGTGGGTTCGCTGGCGGCCATGGGCTTCTCCCGCTTCGCCTTGGCCCA
GGCGAGGCCCGTGAAGCTCGCCGCCCTCCTTCCCCTCACCGGGCCCTTCGCCTTCGCCGGCAACGCCGGCCGGGAAGGGT
TCGTGGACGGGGTGGACTACGTGAACGAGGTCCTGGGGGGGATCGCCGGGCGGAAGCTTGAGCTCATCGTGGAGGACACG
GGCTACGACGTGGCCAAGGGGACGGCCGCCTTCAACCGGGTCCTCTCCAGGGAGCGGCCCGACGAGCTCCTCTTCGTCTA
CGGGGACTCCACGGGGCTTTCCAAGGCCCTGGCCCCGGAGATCGCCAGGATCGGCCTCCCCTACTCCGCCACCAGCTTCG
CCAACGAGCTTGCCGACCCCGAGAAGTACCCCACCATCTTCGTCTTCGGCCCCACCTACAACGACATGATGGAGGCCCTC
CTGCGCCAGATCCGCCTGCAAAAGGGCAAGGCCCGGATCGCCCTCGTCTACTCCAACACGGAGTTCGGCCGCGACCCCAT
CCCCTACGTCAAGGAGCGGGCCAAGGCTTTGGGGATGGAGGTGGTCCACGAGGAGGTGACGCCCCCCGCCTTCACCGACG
CCACCCCCGTGGTCCTGAACCTGCGCCGGGCCAACCCCGACTTCGTCCTCCTCCAGGGCTACGCCCTCTCCGCGGAGCCC
CTGATCCTAAGGGCCGCGCGGGAGCAGGGCCTAAGGGCCCAGTTCATGGGCACCTACTACTCGGCGGAGCTCGTCCTCAT
CCAGCGGGCGGGGCCCGCCGCCGAGGGCTTCACCGTGACCTACCACAACCCCTACTGGTACGACACCCTGGTGCCCGCCG
TGGACGAGATGCGCAAGTTCCGCCAGAGGAAGGGCCGGGACTCCTCCTACCGCCCCACCTACTACATGGGGAGCCTGGCC
GTGGCCCTGGGGGTGGCCGAGGCCATGCGCCGGGCGGCCCAGGCGGGGAAGCTCACCCGGGCCGGGGTGGTGGAGTACCT
GGAGAAGATCGGGGACTACAACGCCATGGGCCTGGTGCGGGGCTACCAGTTCGTGAACCACCGCATCCCCTACACCAAGC
TCTACCGGGCGAGCGTCAAGGACGGGCGCTTCAACGCCATCACCGACTGGCTGAAGCTGGCCTAG

Upstream 100 bases:

>100_bases
CGGCCGCAGCAGCCCCAGGCCGGCTAAATCCCCCCTGGTCTTGCCAAAAGGAGGGGGGCATGCTATCTTTGCCCCAAGCA
CAACCGGAAAGGAGGCGTGC

Downstream 100 bases:

>100_bases
GGCCAGGCCCCTTCCCTCGGGAGGAAGCGTGGCGGACCTCCTTCCCTACCTCATCGGCGGCTTGGCCAACGGGGCCCTCT
ACAGCCTCCTCGCCTTGGGC

Product: branched-chain amino acid ABC transporter amino acid-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-BP [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MKRREFLRRLGVGSLAAMGFSRFALAQARPVKLAALLPLTGPFAFAGNAGREGFVDGVDYVNEVLGGIAGRKLELIVEDT
GYDVAKGTAAFNRVLSRERPDELLFVYGDSTGLSKALAPEIARIGLPYSATSFANELADPEKYPTIFVFGPTYNDMMEAL
LRQIRLQKGKARIALVYSNTEFGRDPIPYVKERAKALGMEVVHEEVTPPAFTDATPVVLNLRRANPDFVLLQGYALSAEP
LILRAAREQGLRAQFMGTYYSAELVLIQRAGPAAEGFTVTYHNPYWYDTLVPAVDEMRKFRQRKGRDSSYRPTYYMGSLA
VALGVAEAMRRAAQAGKLTRAGVVEYLEKIGDYNAMGLVRGYQFVNHRIPYTKLYRASVKDGRFNAITDWLKLA

Sequences:

>Translated_394_residues
MKRREFLRRLGVGSLAAMGFSRFALAQARPVKLAALLPLTGPFAFAGNAGREGFVDGVDYVNEVLGGIAGRKLELIVEDT
GYDVAKGTAAFNRVLSRERPDELLFVYGDSTGLSKALAPEIARIGLPYSATSFANELADPEKYPTIFVFGPTYNDMMEAL
LRQIRLQKGKARIALVYSNTEFGRDPIPYVKERAKALGMEVVHEEVTPPAFTDATPVVLNLRRANPDFVLLQGYALSAEP
LILRAAREQGLRAQFMGTYYSAELVLIQRAGPAAEGFTVTYHNPYWYDTLVPAVDEMRKFRQRKGRDSSYRPTYYMGSLA
VALGVAEAMRRAAQAGKLTRAGVVEYLEKIGDYNAMGLVRGYQFVNHRIPYTKLYRASVKDGRFNAITDWLKLA
>Mature_394_residues
MKRREFLRRLGVGSLAAMGFSRFALAQARPVKLAALLPLTGPFAFAGNAGREGFVDGVDYVNEVLGGIAGRKLELIVEDT
GYDVAKGTAAFNRVLSRERPDELLFVYGDSTGLSKALAPEIARIGLPYSATSFANELADPEKYPTIFVFGPTYNDMMEAL
LRQIRLQKGKARIALVYSNTEFGRDPIPYVKERAKALGMEVVHEEVTPPAFTDATPVVLNLRRANPDFVLLQGYALSAEP
LILRAAREQGLRAQFMGTYYSAELVLIQRAGPAAEGFTVTYHNPYWYDTLVPAVDEMRKFRQRKGRDSSYRPTYYMGSLA
VALGVAEAMRRAAQAGKLTRAGVVEYLEKIGDYNAMGLVRGYQFVNHRIPYTKLYRASVKDGRFNAITDWLKLA

Specific function: This protein is a component of the leucine, isoleucine, valine, (threonine) transport system, which is one of the two periplasmic binding protein-dependent transport systems of the high-affinity transport of the branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=366, Percent_Identity=26.2295081967213, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789867, Length=285, Percent_Identity=26.6666666666667, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 43723; Mature: 43723

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRREFLRRLGVGSLAAMGFSRFALAQARPVKLAALLPLTGPFAFAGNAGREGFVDGVDY
CCHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEECCCCCCCCCCHHHHH
VNEVLGGIAGRKLELIVEDTGYDVAKGTAAFNRVLSRERPDELLFVYGDSTGLSKALAPE
HHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHH
IARIGLPYSATSFANELADPEKYPTIFVFGPTYNDMMEALLRQIRLQKGKARIALVYSNT
HHHHCCCCCHHHHHHHHCCCHHCCEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEEECC
EFGRDPIPYVKERAKALGMEVVHEEVTPPAFTDATPVVLNLRRANPDFVLLQGYALSAEP
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEEECEECCCCH
LILRAAREQGLRAQFMGTYYSAELVLIQRAGPAAEGFTVTYHNPYWYDTLVPAVDEMRKF
HHHHHHHHCCCHHHHHHHEEEEEEEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHH
RQRKGRDSSYRPTYYMGSLAVALGVAEAMRRAAQAGKLTRAGVVEYLEKIGDYNAMGLVR
HHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHH
GYQFVNHRIPYTKLYRASVKDGRFNAITDWLKLA
HHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKRREFLRRLGVGSLAAMGFSRFALAQARPVKLAALLPLTGPFAFAGNAGREGFVDGVDY
CCHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEECCCCCCCCCCHHHHH
VNEVLGGIAGRKLELIVEDTGYDVAKGTAAFNRVLSRERPDELLFVYGDSTGLSKALAPE
HHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHH
IARIGLPYSATSFANELADPEKYPTIFVFGPTYNDMMEALLRQIRLQKGKARIALVYSNT
HHHHCCCCCHHHHHHHHCCCHHCCEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEEECC
EFGRDPIPYVKERAKALGMEVVHEEVTPPAFTDATPVVLNLRRANPDFVLLQGYALSAEP
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEEECEECCCCH
LILRAAREQGLRAQFMGTYYSAELVLIQRAGPAAEGFTVTYHNPYWYDTLVPAVDEMRKF
HHHHHHHHCCCHHHHHHHEEEEEEEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHH
RQRKGRDSSYRPTYYMGSLAVALGVAEAMRRAAQAGKLTRAGVVEYLEKIGDYNAMGLVR
HHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHH
GYQFVNHRIPYTKLYRASVKDGRFNAITDWLKLA
HHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA