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The map label for this gene is gatA [H]

Identifier: 55980542

GI number: 55980542

Start: 540483

End: 541898

Strand: Direct

Name: gatA [H]

Synonym: TTHA0573

Alternate gene names: 55980542

Gene position: 540483-541898 (Clockwise)

Preceding gene: 55980541

Following gene: 55980543

Centisome position: 29.22

GC content: 72.88

Gene sequence:

>1416_bases
ATGTTGGCCCACGAGATCCGCGCCCGCGTGGCCCGGGGGGAGGTTTCCCCCCTGGAGGTGGCCCAGGCCTACCTGAAACG
GGTCCAGGAGCTGGACCCGGGCCTCGGCGCCTTCCTCTCCCTGAACGAGAGGCTTTTGGAGGAGGCGGAGGCGGTGGACC
CCGGGCTTCCCCTGGCGGGCCTCGTGGTGGCGGTGAAGGACAACATCGCCACCCGTGGCCTCCGCACCACGGCGGGAAGC
CGCCTTCTGGAGAACTTCGTGCCCCCCTACGAGGCCACCGCGGTGGCGAGGCTTAAGGCCCTGGGCGCCCTGGTCCTGGG
CAAGACCAACCTGGACGAGTTCGGCATGGGCTCCTCCACGGAGCACTCCGCCTTCTTTCCCACCAAGAACCCCTTTGACC
CGGATAGGGTCCCCGGGGGCTCCAGCGGGGGAAGCGCCGCCGCCTTGGCCGCCGACCTCGCCCCCCTCGCCCTGGGCTCG
GACACGGGGGGAAGCGTCCGGCAGCCCGCCGCCTTCTGCGGCGTCTACGGCCTCAAGCCCACCTACGGCCGGGTGAGCCG
CTTCGGCCTCATCGCCTACGCCTCGAGCCTGGACCAGATCGGCCCCATGGCCCGCTCCGTGCGGGACCTGGCCCTCCTCA
TGGACGCCGCGGCCGGGCCCGACCCCCTGGACGCCACGAGCCTGGACCTCCCCCCCCGCTTCCAGGAGGCCCTGGAGGGG
CCCCTTCCCCCCCTGCGCCTCGGGGTGGTGCGGGAGGCCCTCGCGGGGAATAGCCCCGGGGTGGAGAGGGCCCTGGAGGA
GGCCCTTAAGGTCTTCCGGGAGCTTGGCCTTTCCGTGCGCGAGGTCTCCTGGCCTTCCCTCCCCCAGGCCCTCGCCGCCT
ACTACATCCTGGCCCCGGCGGAGGCGAGCTCCAACCTGGCCCGCTACGACGGCACCCTCTACGGGCGGCGGGCGGCGGGG
GAGGAGGTGGAGGGGATGATGGAGGCCACCCGGGCCCTCTTCGGCCTCGAGGTGAAAAGGCGCGTCCTCGTGGGCACCTT
CGTCCTCTCTAGCGGCTACTACGAGGCCTACTATGGCCGCGCCCAGGCCTTCCGCCGCCGCCTCAAGGCCGAGGCCCAGG
CCCTCTTCCGGGAGGTGGACCTCCTCCTCCTCCCCACCACCCCCCACCCCGCCTTCCCCTTCGGGGCCCGGCGCGATCCC
CTCGCCATGTACCGGGAGGACCTCTACACCGTGGGGGCGAACCTCACGGGCCTGCCCGCCCTCTCCTTCCCCGCGGGGTT
TGAGGGGCACCTGCCCGTGGGCCTCCAGCTCCTCGCCCCCTGGGGGGAGGACGAGAGGCTTCTTCGGGCGGCCCTCGCCT
TTGAGGAGGCCACGGCCCGGGCCCACCTCAAGGCCCCCTTGGGGGAGGCCCTCTGA

Upstream 100 bases:

>100_bases
CACGAGCCCGGGGACGTGGTCTACCTGCCCCGGGGGGGCGAGGAGGCCTACGTGGGCTTCCGGGACTGGCTTTGCCTCCA
CTTCGGGCTATAGTGGGGGC

Downstream 100 bases:

>100_bases
TGGAGGCCGCCTTCCGCTACCGCATGGCCACCCGGCTCGCCCGCAGGCTCAGGGAGGCCGGGCGCCCCCTTCCCCTCCCT
GCCCTGGGGGAGGCCTTGGG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MLAHEIRARVARGEVSPLEVAQAYLKRVQELDPGLGAFLSLNERLLEEAEAVDPGLPLAGLVVAVKDNIATRGLRTTAGS
RLLENFVPPYEATAVARLKALGALVLGKTNLDEFGMGSSTEHSAFFPTKNPFDPDRVPGGSSGGSAAALAADLAPLALGS
DTGGSVRQPAAFCGVYGLKPTYGRVSRFGLIAYASSLDQIGPMARSVRDLALLMDAAAGPDPLDATSLDLPPRFQEALEG
PLPPLRLGVVREALAGNSPGVERALEEALKVFRELGLSVREVSWPSLPQALAAYYILAPAEASSNLARYDGTLYGRRAAG
EEVEGMMEATRALFGLEVKRRVLVGTFVLSSGYYEAYYGRAQAFRRRLKAEAQALFREVDLLLLPTTPHPAFPFGARRDP
LAMYREDLYTVGANLTGLPALSFPAGFEGHLPVGLQLLAPWGEDERLLRAALAFEEATARAHLKAPLGEAL

Sequences:

>Translated_471_residues
MLAHEIRARVARGEVSPLEVAQAYLKRVQELDPGLGAFLSLNERLLEEAEAVDPGLPLAGLVVAVKDNIATRGLRTTAGS
RLLENFVPPYEATAVARLKALGALVLGKTNLDEFGMGSSTEHSAFFPTKNPFDPDRVPGGSSGGSAAALAADLAPLALGS
DTGGSVRQPAAFCGVYGLKPTYGRVSRFGLIAYASSLDQIGPMARSVRDLALLMDAAAGPDPLDATSLDLPPRFQEALEG
PLPPLRLGVVREALAGNSPGVERALEEALKVFRELGLSVREVSWPSLPQALAAYYILAPAEASSNLARYDGTLYGRRAAG
EEVEGMMEATRALFGLEVKRRVLVGTFVLSSGYYEAYYGRAQAFRRRLKAEAQALFREVDLLLLPTTPHPAFPFGARRDP
LAMYREDLYTVGANLTGLPALSFPAGFEGHLPVGLQLLAPWGEDERLLRAALAFEEATARAHLKAPLGEAL
>Mature_471_residues
MLAHEIRARVARGEVSPLEVAQAYLKRVQELDPGLGAFLSLNERLLEEAEAVDPGLPLAGLVVAVKDNIATRGLRTTAGS
RLLENFVPPYEATAVARLKALGALVLGKTNLDEFGMGSSTEHSAFFPTKNPFDPDRVPGGSSGGSAAALAADLAPLALGS
DTGGSVRQPAAFCGVYGLKPTYGRVSRFGLIAYASSLDQIGPMARSVRDLALLMDAAAGPDPLDATSLDLPPRFQEALEG
PLPPLRLGVVREALAGNSPGVERALEEALKVFRELGLSVREVSWPSLPQALAAYYILAPAEASSNLARYDGTLYGRRAAG
EEVEGMMEATRALFGLEVKRRVLVGTFVLSSGYYEAYYGRAQAFRRRLKAEAQALFREVDLLLLPTTPHPAFPFGARRDP
LAMYREDLYTVGANLTGLPALSFPAGFEGHLPVGLQLLAPWGEDERLLRAALAFEEATARAHLKAPLGEAL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=490, Percent_Identity=36.3265306122449, Blast_Score=265, Evalue=1e-70,
Organism=Homo sapiens, GI195972892, Length=489, Percent_Identity=26.9938650306748, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI166795287, Length=286, Percent_Identity=32.1678321678322, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=408, Percent_Identity=37.7450980392157, Blast_Score=244, Evalue=5e-65,
Organism=Caenorhabditis elegans, GI17537465, Length=479, Percent_Identity=26.5135699373695, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI71990152, Length=325, Percent_Identity=31.0769230769231, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17556264, Length=280, Percent_Identity=30.7142857142857, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17538252, Length=309, Percent_Identity=27.831715210356, Blast_Score=98, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17538254, Length=426, Percent_Identity=22.5352112676056, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17556276, Length=278, Percent_Identity=29.8561151079137, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17556278, Length=454, Percent_Identity=26.8722466960352, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=454, Percent_Identity=38.5462555066079, Blast_Score=266, Evalue=6e-72,
Organism=Saccharomyces cerevisiae, GI6319685, Length=443, Percent_Identity=30.2483069977427, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320448, Length=227, Percent_Identity=29.9559471365639, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648113, Length=483, Percent_Identity=38.9233954451346, Blast_Score=265, Evalue=6e-71,
Organism=Drosophila melanogaster, GI24652985, Length=484, Percent_Identity=25.6198347107438, Blast_Score=132, Evalue=8e-31,
Organism=Drosophila melanogaster, GI19922090, Length=484, Percent_Identity=25.6198347107438, Blast_Score=132, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24652981, Length=484, Percent_Identity=25.6198347107438, Blast_Score=132, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24652983, Length=484, Percent_Identity=25.6198347107438, Blast_Score=132, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24648435, Length=491, Percent_Identity=27.0875763747454, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24648437, Length=491, Percent_Identity=27.0875763747454, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI45550774, Length=491, Percent_Identity=27.0875763747454, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24644968, Length=499, Percent_Identity=26.4529058116232, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI161078093, Length=490, Percent_Identity=27.1428571428571, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24648441, Length=432, Percent_Identity=27.7777777777778, Blast_Score=115, Evalue=9e-26,
Organism=Drosophila melanogaster, GI24648439, Length=432, Percent_Identity=27.7777777777778, Blast_Score=115, Evalue=9e-26,
Organism=Drosophila melanogaster, GI21356731, Length=481, Percent_Identity=27.2349272349272, Blast_Score=112, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 50228; Mature: 50228

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAHEIRARVARGEVSPLEVAQAYLKRVQELDPGLGAFLSLNERLLEEAEAVDPGLPLAG
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHH
LVVAVKDNIATRGLRTTAGSRLLENFVPPYEATAVARLKALGALVLGKTNLDEFGMGSST
EEEEECCCHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCC
EHSAFFPTKNPFDPDRVPGGSSGGSAAALAADLAPLALGSDTGGSVRQPAAFCGVYGLKP
CCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHEECCCCCCCCCCCHHHHHCCCCCC
TYGRVSRFGLIAYASSLDQIGPMARSVRDLALLMDAAAGPDPLDATSLDLPPRFQEALEG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCC
PLPPLRLGVVREALAGNSPGVERALEEALKVFRELGLSVREVSWPSLPQALAAYYILAPA
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHCCCC
EASSNLARYDGTLYGRRAAGEEVEGMMEATRALFGLEVKRRVLVGTFVLSSGYYEAYYGR
CCCCCHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
AQAFRRRLKAEAQALFREVDLLLLPTTPHPAFPFGARRDPLAMYREDLYTVGANLTGLPA
HHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
LSFPAGFEGHLPVGLQLLAPWGEDERLLRAALAFEEATARAHLKAPLGEAL
CCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLAHEIRARVARGEVSPLEVAQAYLKRVQELDPGLGAFLSLNERLLEEAEAVDPGLPLAG
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHH
LVVAVKDNIATRGLRTTAGSRLLENFVPPYEATAVARLKALGALVLGKTNLDEFGMGSST
EEEEECCCHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCC
EHSAFFPTKNPFDPDRVPGGSSGGSAAALAADLAPLALGSDTGGSVRQPAAFCGVYGLKP
CCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHEECCCCCCCCCCCHHHHHCCCCCC
TYGRVSRFGLIAYASSLDQIGPMARSVRDLALLMDAAAGPDPLDATSLDLPPRFQEALEG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCC
PLPPLRLGVVREALAGNSPGVERALEEALKVFRELGLSVREVSWPSLPQALAAYYILAPA
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHCCCC
EASSNLARYDGTLYGRRAAGEEVEGMMEATRALFGLEVKRRVLVGTFVLSSGYYEAYYGR
CCCCCHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
AQAFRRRLKAEAQALFREVDLLLLPTTPHPAFPFGARRDPLAMYREDLYTVGANLTGLPA
HHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
LSFPAGFEGHLPVGLQLLAPWGEDERLLRAALAFEEATARAHLKAPLGEAL
CCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA