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The map label for this gene is nfrA1 [H]

Identifier: 55980236

GI number: 55980236

Start: 254203

End: 255123

Strand: Reverse

Name: nfrA1 [H]

Synonym: TTHA0267

Alternate gene names: 55980236

Gene position: 255123-254203 (Counterclockwise)

Preceding gene: 55980237

Following gene: 55980225

Centisome position: 13.79

GC content: 71.01

Gene sequence:

>921_bases
GTGGAACTCCTAGACGTCCTGGCGAGAAGGCGGAGCGTACGCCGCTTCAAGCCCCTTCCCCTTCCCGAGGAGGACCTGGA
AAAGCTCCTCTTCGCCCTCCAGCGGGCCCCCACCGACGCGAGCGCCCAGCTCTACTCGGCGATCCGGGTACGGGATCCAA
GGCTTAGGGACGAGGTGGCCCGGCTCTCCGGGGACCAGGAGCACATCAGGCAGGCGGCGGAGTTCTTCCTCTTCCTCGCA
GACGTCCACCGCCTGGAGCGGCTCCTCGCCCACCGGGGGCAGAGGATGGCCTTCTGGCCCAGGACCGCCCTCCACTTCGC
CATCCTGGACGCGGGGATCGCCGCCTCGTACCTCGCCCTCACCGCCGAGGCCTTAGGCTACGGGGTCTGCTTCATCGGCG
GGGTCTTGAACGGGGTGGAGACCCTCACGGACCTCCTCGGGCTTCCCCCGGGGGTCCTCCCCGTGGTGGGGCTTGCCGTT
GGGGTGCCCGACGAGGAGGGCCCCCCGAGGCCCAGGCTTCCCCGGAGGCTCGTGGTCCACGAGGACCGCTACCGCCCCTA
CGCCGCGGAGGACCTGGAGGAGGCCTACCGGGCCATGGCCCCCTACAGCCGGGTGGGGGACTGGAACCGGGTCCTTAGGC
GCTACTTCGCCGAGGGGGGCACGATGGAGGCCAGGGAGAAGCCTTACGGGAAGGTCCTTTCCCGGCAGGGGTTTGACCCG
GACCTGCCCAAAGGGGCCCCCTTCTACTCCCTGGGGGCCTTGCTGGAGGAGGCCTTGGCGGAGGCCCGGGGGGTGCTCTT
CCGCAAGGGGGAGGTCTGGCTGGAGGAGGAGGCCCGGGCCTTCCGGGGGGAGGGGAGGCCGGGGGAGGCCCTCCTCATGG
CGTTGCGGAAGGCGCGGGGGGAGGTTCCGGACTGGCCATGA

Upstream 100 bases:

>100_bases
CCCGAGGACCCCAGAAGCCGCCTCTACCTGGAACGGCAGGGGCTTTTCGGGTGGAAGGTGGTGCGGATGGAGCTCCCCTT
GGAGTAGTATCCTGGCCCCC

Downstream 100 bases:

>100_bases
CCCGCCTCGCCCCCCGGTAGACCTTCCGGTAGAAGGGGGCCTCGAGGCTCTCTATGACCACCCGGCTTCCGTAGCTAGAG
GCGTGGGCGAAGACCCCCCG

Product: oxygen-insensitive NADPH nitroreductase

Products: NA

Alternate protein names: NADPH-dependent FMN reductase; NADPH-dependent nitroreductase; NADPH-dependent oxidoreductase [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MELLDVLARRRSVRRFKPLPLPEEDLEKLLFALQRAPTDASAQLYSAIRVRDPRLRDEVARLSGDQEHIRQAAEFFLFLA
DVHRLERLLAHRGQRMAFWPRTALHFAILDAGIAASYLALTAEALGYGVCFIGGVLNGVETLTDLLGLPPGVLPVVGLAV
GVPDEEGPPRPRLPRRLVVHEDRYRPYAAEDLEEAYRAMAPYSRVGDWNRVLRRYFAEGGTMEAREKPYGKVLSRQGFDP
DLPKGAPFYSLGALLEEALAEARGVLFRKGEVWLEEEARAFRGEGRPGEALLMALRKARGEVPDWP

Sequences:

>Translated_306_residues
MELLDVLARRRSVRRFKPLPLPEEDLEKLLFALQRAPTDASAQLYSAIRVRDPRLRDEVARLSGDQEHIRQAAEFFLFLA
DVHRLERLLAHRGQRMAFWPRTALHFAILDAGIAASYLALTAEALGYGVCFIGGVLNGVETLTDLLGLPPGVLPVVGLAV
GVPDEEGPPRPRLPRRLVVHEDRYRPYAAEDLEEAYRAMAPYSRVGDWNRVLRRYFAEGGTMEAREKPYGKVLSRQGFDP
DLPKGAPFYSLGALLEEALAEARGVLFRKGEVWLEEEARAFRGEGRPGEALLMALRKARGEVPDWP
>Mature_306_residues
MELLDVLARRRSVRRFKPLPLPEEDLEKLLFALQRAPTDASAQLYSAIRVRDPRLRDEVARLSGDQEHIRQAAEFFLFLA
DVHRLERLLAHRGQRMAFWPRTALHFAILDAGIAASYLALTAEALGYGVCFIGGVLNGVETLTDLLGLPPGVLPVVGLAV
GVPDEEGPPRPRLPRRLVVHEDRYRPYAAEDLEEAYRAMAPYSRVGDWNRVLRRYFAEGGTMEAREKPYGKVLSRQGFDP
DLPKGAPFYSLGALLEEALAEARGVLFRKGEVWLEEEARAFRGEGRPGEALLMALRKARGEVPDWP

Specific function: Reduces FMNH(2) to FMN, with NADPH as reductant. It also reduces nitroaromatic compounds, quinones and azo dyes [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=218, Percent_Identity=32.5688073394495, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 34360; Mature: 34360

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLDVLARRRSVRRFKPLPLPEEDLEKLLFALQRAPTDASAQLYSAIRVRDPRLRDEVA
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHH
RLSGDQEHIRQAAEFFLFLADVHRLERLLAHRGQRMAFWPRTALHFAILDAGIAASYLAL
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHH
TAEALGYGVCFIGGVLNGVETLTDLLGLPPGVLPVVGLAVGVPDEEGPPRPRLPRRLVVH
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHC
EDRYRPYAAEDLEEAYRAMAPYSRVGDWNRVLRRYFAEGGTMEAREKPYGKVLSRQGFDP
CCCCCCCHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHCCCCCCHHHCHHHHHHHCCCCCC
DLPKGAPFYSLGALLEEALAEARGVLFRKGEVWLEEEARAFRGEGRPGEALLMALRKARG
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
EVPDWP
CCCCCC
>Mature Secondary Structure
MELLDVLARRRSVRRFKPLPLPEEDLEKLLFALQRAPTDASAQLYSAIRVRDPRLRDEVA
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHH
RLSGDQEHIRQAAEFFLFLADVHRLERLLAHRGQRMAFWPRTALHFAILDAGIAASYLAL
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHH
TAEALGYGVCFIGGVLNGVETLTDLLGLPPGVLPVVGLAVGVPDEEGPPRPRLPRRLVVH
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHC
EDRYRPYAAEDLEEAYRAMAPYSRVGDWNRVLRRYFAEGGTMEAREKPYGKVLSRQGFDP
CCCCCCCHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHCCCCCCHHHCHHHHHHHCCCCCC
DLPKGAPFYSLGALLEEALAEARGVLFRKGEVWLEEEARAFRGEGRPGEALLMALRKARG
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
EVPDWP
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 9836433 [H]