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The map label for this gene is lipA

Identifier: 55980208

GI number: 55980208

Start: 232057

End: 233028

Strand: Reverse

Name: lipA

Synonym: TTHA0239

Alternate gene names: 55980208

Gene position: 233028-232057 (Counterclockwise)

Preceding gene: 55980209

Following gene: 55980207

Centisome position: 12.6

GC content: 68.62

Gene sequence:

>972_bases
ATGAAGCCTAAGTTTGAGACGGTGGAGCTCCTCTCCCCCACGGGGGAGGTCGTTGAGCTCAAGGTGGTGAAGCGGGGCCT
GGCCCAGGCCCGGCCCGAGCCCGTGGACCGGAACAAGCCCGCCTGGATCAAGGCCCCTTTGCCCACCGGGCCCAGATACC
AGGCCCTGAAGGCCATGGTGCGGGAGCTTAGGCTCCACACCGTCTGCCAGGAGGCCCTCTGCCCCAACATCGGGGAGTGC
TGGACCCACGGGACCCTGACGGTGATGATCCTGGGGGATATCTGCACCCGGGCCTGCAAGTTCTGCGCCGTCCACACCGG
GAACCCCAAGGGCCTCGTGGACCCGGAGGAACCAAGGAGGGTGGCCGAGGCCATCGCCCGCATGGGGGTGCGGTACGTGG
TCTTGACCAGCGTGGACCGGGATGACCTTCCGGATGGCGGCGCCTCCCACTTCGCCGCCACCATCCGGGCCATCAAGGAG
AGGGCCCCCGGGGTCTTGGTGGAGGCCCTGACCCCTGACTTCCAGGGGGACCTGAAGGCCGTGGAGACGGTCCTCGCCGC
GAACCCCGAGGTCTACGCCCAGAACCTGGAGACGGTGCGCCGCCTCACCCCCAAGGTCCGCGACCCCCGGGCGGGGTACG
AGCAGACCCTGAAGGTTCTCGCCCACGCCAAGAAGGTCCGGCCCGACATCCTCACCAAGTCTAGCCTCATGCTGGGCCTG
GGGGAGACGGAGGAGGAGATCCTCGAGGCCATGCGGGACCTCAGGGCCGCGGGGGTGGACATCCTCACCTTGGGCCAGTA
CCTGAGGCCCACCCCTGCCCACCTCCCCGTGGCCCGCTACGTTCCCCCCGAGGACTTCAAGCGGTACGAGGCCTGGGGGT
ACGAGCTGGGCTTTAGGGAGGTCTTCGCCGGGCCCTTGGTGCGGAGCTCCTACCGGGCGGACCGGGTCTTCCTGGAGGCC
ACCCAAAGATGA

Upstream 100 bases:

>100_bases
AAGCTTTTGGGCCGGAAGGTCCCCATGGAGGAGGCCAAGGCCAGGGTGGTGGCGGCCTTCGCCGAGGTCTTCGGCCTGAG
GCCCGTAGAGGGGAGTGTCC

Downstream 100 bases:

>100_bases
GCCGAAAGACGGCCCTTTTCCTCCTGGACCTCCTCGCCCTCCTCCTCTTCGCCGGGGTGGGCCTCCTTTCCCACGGGCTT
CCCCTCTCCTTGGGGGGCCT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MKPKFETVELLSPTGEVVELKVVKRGLAQARPEPVDRNKPAWIKAPLPTGPRYQALKAMVRELRLHTVCQEALCPNIGEC
WTHGTLTVMILGDICTRACKFCAVHTGNPKGLVDPEEPRRVAEAIARMGVRYVVLTSVDRDDLPDGGASHFAATIRAIKE
RAPGVLVEALTPDFQGDLKAVETVLAANPEVYAQNLETVRRLTPKVRDPRAGYEQTLKVLAHAKKVRPDILTKSSLMLGL
GETEEEILEAMRDLRAAGVDILTLGQYLRPTPAHLPVARYVPPEDFKRYEAWGYELGFREVFAGPLVRSSYRADRVFLEA
TQR

Sequences:

>Translated_323_residues
MKPKFETVELLSPTGEVVELKVVKRGLAQARPEPVDRNKPAWIKAPLPTGPRYQALKAMVRELRLHTVCQEALCPNIGEC
WTHGTLTVMILGDICTRACKFCAVHTGNPKGLVDPEEPRRVAEAIARMGVRYVVLTSVDRDDLPDGGASHFAATIRAIKE
RAPGVLVEALTPDFQGDLKAVETVLAANPEVYAQNLETVRRLTPKVRDPRAGYEQTLKVLAHAKKVRPDILTKSSLMLGL
GETEEEILEAMRDLRAAGVDILTLGQYLRPTPAHLPVARYVPPEDFKRYEAWGYELGFREVFAGPLVRSSYRADRVFLEA
TQR
>Mature_323_residues
MKPKFETVELLSPTGEVVELKVVKRGLAQARPEPVDRNKPAWIKAPLPTGPRYQALKAMVRELRLHTVCQEALCPNIGEC
WTHGTLTVMILGDICTRACKFCAVHTGNPKGLVDPEEPRRVAEAIARMGVRYVVLTSVDRDDLPDGGASHFAATIRAIKE
RAPGVLVEALTPDFQGDLKAVETVLAANPEVYAQNLETVRRLTPKVRDPRAGYEQTLKVLAHAKKVRPDILTKSSLMLGL
GETEEEILEAMRDLRAAGVDILTLGQYLRPTPAHLPVARYVPPEDFKRYEAWGYELGFREVFAGPLVRSSYRADRVFLEA
TQR

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=285, Percent_Identity=55.7894736842105, Blast_Score=336, Evalue=2e-92,
Organism=Homo sapiens, GI37577164, Length=243, Percent_Identity=56.3786008230453, Blast_Score=291, Evalue=7e-79,
Organism=Escherichia coli, GI1786846, Length=278, Percent_Identity=52.8776978417266, Blast_Score=287, Evalue=6e-79,
Organism=Caenorhabditis elegans, GI32564533, Length=262, Percent_Identity=51.9083969465649, Blast_Score=287, Evalue=4e-78,
Organism=Saccharomyces cerevisiae, GI6324770, Length=299, Percent_Identity=52.1739130434783, Blast_Score=320, Evalue=1e-88,
Organism=Drosophila melanogaster, GI221513272, Length=286, Percent_Identity=54.5454545454545, Blast_Score=329, Evalue=2e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_THET2 (Q72GV1)

Other databases:

- EMBL:   AE017221
- RefSeq:   YP_005716.1
- ProteinModelPortal:   Q72GV1
- STRING:   Q72GV1
- GeneID:   2775045
- GenomeReviews:   AE017221_GR
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- PhylomeDB:   Q72GV1
- ProtClustDB:   PRK05481
- BioCyc:   TTHE262724:TT_C1747-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 35909; Mature: 35909

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPKFETVELLSPTGEVVELKVVKRGLAQARPEPVDRNKPAWIKAPLPTGPRYQALKAMV
CCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCHHHHHHHHH
RELRLHTVCQEALCPNIGECWTHGTLTVMILGDICTRACKFCAVHTGNPKGLVDPEEPRR
HHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHH
VAEAIARMGVRYVVLTSVDRDDLPDGGASHFAATIRAIKERAPGVLVEALTPDFQGDLKA
HHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHH
VETVLAANPEVYAQNLETVRRLTPKVRDPRAGYEQTLKVLAHAKKVRPDILTKSSLMLGL
HHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCC
GETEEEILEAMRDLRAAGVDILTLGQYLRPTPAHLPVARYVPPEDFKRYEAWGYELGFRE
CCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHHCHHCCHHH
VFAGPLVRSSYRADRVFLEATQR
HHHCHHHHHHHHHHHHHHEECCC
>Mature Secondary Structure
MKPKFETVELLSPTGEVVELKVVKRGLAQARPEPVDRNKPAWIKAPLPTGPRYQALKAMV
CCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCHHHHHHHHH
RELRLHTVCQEALCPNIGECWTHGTLTVMILGDICTRACKFCAVHTGNPKGLVDPEEPRR
HHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHH
VAEAIARMGVRYVVLTSVDRDDLPDGGASHFAATIRAIKERAPGVLVEALTPDFQGDLKA
HHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHH
VETVLAANPEVYAQNLETVRRLTPKVRDPRAGYEQTLKVLAHAKKVRPDILTKSSLMLGL
HHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCC
GETEEEILEAMRDLRAAGVDILTLGQYLRPTPAHLPVARYVPPEDFKRYEAWGYELGFRE
CCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHHCHHCCHHH
VFAGPLVRSSYRADRVFLEATQR
HHHCHHHHHHHHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA