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The map label for this gene is lipB

Identifier: 55980209

GI number: 55980209

Start: 233018

End: 233671

Strand: Reverse

Name: lipB

Synonym: TTHA0240

Alternate gene names: 55980209

Gene position: 233671-233018 (Counterclockwise)

Preceding gene: 55980210

Following gene: 55980208

Centisome position: 12.63

GC content: 67.43

Gene sequence:

>654_bases
GTGGAGTTCCTGGTGGAGGACCTCGGCTTGGTGCCTTACGGGGAGGCCTGGGCGTACCAGAAGCGGGTGCACCGGGAGGT
GGTGGCGGGAAACCGCCCCCCCACCCTCCTCCTCCTGGAGCACCCCAGGGTCATCACCTTAGGCCGGAAGGCCACGGGGG
AGAACCTGCTCTTCCCCGAGAGCTGGTACCGGGAGAACGGGTTTGAGCTCTACTGGGTGGAGCGGGGCGGGGACGTGACC
TACCACGGCCCCGGGCAGCTGGTGGGCTACCCCATCTTCCCCGTGGGCCGGGAGGTGCGCCGCTTTTTAAGGCAGATTGA
GGAGGCCATCGTCCGGGTGGCCGCGGGCTACGGGATCTCCGCCTACCCCACCCCGGGCTACGCCGGAGTCTGGGTGGGGG
AGGACAAGCTCTGCGCCATCGGCGTGGCGGTGAAGGAGGGGGTGAGCTTCCACGGCTTCGCCCTGAACGTCAACACCGAC
CTCAACGACTTCACCGTCATCGTCCCCTGCGGCCTCAAGGGAAAGGGGGTCACCTCCTTGGAGAAGCTTTTGGGCCGGAA
GGTCCCCATGGAGGAGGCCAAGGCCAGGGTGGTGGCGGCCTTCGCCGAGGTCTTCGGCCTGAGGCCCGTAGAGGGGAGTG
TCCATGAAGCCTAA

Upstream 100 bases:

>100_bases
GCTAGCCAGGGTCTCCCATGTTGCGAAGCCAAGGCGGGCGCTTAGGGGCTTTGTGCCCCGGCCTTTGGACACGGGGGCCT
GGGCGGCGTATCCTTAGGGC

Downstream 100 bases:

>100_bases
GTTTGAGACGGTGGAGCTCCTCTCCCCCACGGGGGAGGTCGTTGAGCTCAAGGTGGTGAAGCGGGGCCTGGCCCAGGCCC
GGCCCGAGCCCGTGGACCGG

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MEFLVEDLGLVPYGEAWAYQKRVHREVVAGNRPPTLLLLEHPRVITLGRKATGENLLFPESWYRENGFELYWVERGGDVT
YHGPGQLVGYPIFPVGREVRRFLRQIEEAIVRVAAGYGISAYPTPGYAGVWVGEDKLCAIGVAVKEGVSFHGFALNVNTD
LNDFTVIVPCGLKGKGVTSLEKLLGRKVPMEEAKARVVAAFAEVFGLRPVEGSVHEA

Sequences:

>Translated_217_residues
MEFLVEDLGLVPYGEAWAYQKRVHREVVAGNRPPTLLLLEHPRVITLGRKATGENLLFPESWYRENGFELYWVERGGDVT
YHGPGQLVGYPIFPVGREVRRFLRQIEEAIVRVAAGYGISAYPTPGYAGVWVGEDKLCAIGVAVKEGVSFHGFALNVNTD
LNDFTVIVPCGLKGKGVTSLEKLLGRKVPMEEAKARVVAAFAEVFGLRPVEGSVHEA
>Mature_217_residues
MEFLVEDLGLVPYGEAWAYQKRVHREVVAGNRPPTLLLLEHPRVITLGRKATGENLLFPESWYRENGFELYWVERGGDVT
YHGPGQLVGYPIFPVGREVRRFLRQIEEAIVRVAAGYGISAYPTPGYAGVWVGEDKLCAIGVAVKEGVSFHGFALNVNTD
LNDFTVIVPCGLKGKGVTSLEKLLGRKVPMEEAKARVVAAFAEVFGLRPVEGSVHEA

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=215, Percent_Identity=39.0697674418605, Blast_Score=106, Evalue=1e-23,
Organism=Escherichia coli, GI87081767, Length=184, Percent_Identity=35.8695652173913, Blast_Score=112, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6323268, Length=217, Percent_Identity=28.110599078341, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21355483, Length=210, Percent_Identity=39.0476190476191, Blast_Score=138, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_THET2 (Q72GV2)

Other databases:

- EMBL:   AE017221
- RefSeq:   YP_005715.1
- ProteinModelPortal:   Q72GV2
- SMR:   Q72GV2
- STRING:   Q72GV2
- GeneID:   2774724
- GenomeReviews:   AE017221_GR
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   DLKRRRP
- PhylomeDB:   Q72GV2
- ProtClustDB:   CLSK926550
- BioCyc:   TTHE262724:TT_C1746-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 23924; Mature: 23924

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 170-170

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFLVEDLGLVPYGEAWAYQKRVHREVVAGNRPPTLLLLEHPRVITLGRKATGENLLFPE
CCCCHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEECCCCCCCCCCCCH
SWYRENGFELYWVERGGDVTYHGPGQLVGYPIFPVGREVRRFLRQIEEAIVRVAAGYGIS
HHHCCCCCEEEEEECCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCC
AYPTPGYAGVWVGEDKLCAIGVAVKEGVSFHGFALNVNTDLNDFTVIVPCGLKGKGVTSL
CCCCCCCEEEEECCCCEEEEEEEHHCCCEEEEEEEEECCCCCCEEEEEECCCCCCCHHHH
EKLLGRKVPMEEAKARVVAAFAEVFGLRPVEGSVHEA
HHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MEFLVEDLGLVPYGEAWAYQKRVHREVVAGNRPPTLLLLEHPRVITLGRKATGENLLFPE
CCCCHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEECCCCCCCCCCCCH
SWYRENGFELYWVERGGDVTYHGPGQLVGYPIFPVGREVRRFLRQIEEAIVRVAAGYGIS
HHHCCCCCEEEEEECCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCC
AYPTPGYAGVWVGEDKLCAIGVAVKEGVSFHGFALNVNTDLNDFTVIVPCGLKGKGVTSL
CCCCCCCEEEEECCCCEEEEEEEHHCCCEEEEEEEEECCCCCCEEEEEECCCCCCCHHHH
EKLLGRKVPMEEAKARVVAAFAEVFGLRPVEGSVHEA
HHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA