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The map label for this gene is nqo1

Identifier: 55980058

GI number: 55980058

Start: 87066

End: 88382

Strand: Direct

Name: nqo1

Synonym: TTHA0089

Alternate gene names: 55980058

Gene position: 87066-88382 (Clockwise)

Preceding gene: 55980057

Following gene: 55980059

Centisome position: 4.71

GC content: 67.43

Gene sequence:

>1317_bases
ATGACTGGCCCCATTCTTTCCGGACTGGACCCCCGGTTTGAAAGGACCCTCTACGCCCACGTGGGGAAGGAGGGGTCCTG
GACCCTGGACTACTACCTCCGGCACGGGGGGTACGAGACGGCGAAGCGGGTGCTCAAGGAGAAGACCCCGGACGAGGTCA
TAGAGGAGGTGAAGCGCTCCGGGCTTAGGGGCCGGGGCGGGGCGGGCTTCCCCACGGGGCTTAAGTGGAGCTTCATGCCC
AAGGACGACGGGAAGCAGCACTACCTCATCTGCAACGCCGACGAGTCCGAGCCCGGGAGCTTCAAGGACCGCTACATCCT
GGAGGACGTCCCCCACCTCCTCATTGAGGGGATGATCCTCGCCGGGTACGCCATCCGGGCCACGGTGGGCTACATCTACG
TCCGCGGGGAGTACCGCAGGGCGGCGGACCGGCTAGAACAAGCCATCAAGGAGGCCCGGGCCCGGGGCTACCTGGGGAAG
AACCTCTTCGGCACGGACTTCTCCTTTGACCTCCACGTCCACCGGGGGGCCGGGGCCTACATCTGCGGCGAGGAGACGGC
CCTCATGAACTCCCTGGAGGGCCTAAGGGCGAACCCCCGCTTGAAGCCCCCCTTCCCCGCCCAGTCGGGCCTTTGGGGCA
AGCCCACCACCATCAACAACGTGGAGACCCTGGCCTCCGTGGTGCCCATCATGGAACGGGGCGCCGACTGGTTCGCCCAG
ATGGGCACGGAGCAGTCCAAGGGGATGAAGCTCTACCAGATCTCCGGGCCCGTGAAGCGCCCCGGGGTGTACGAGCTCCC
CATGGGCACCACGTTCCGCGAGCTCATCTACGAGTGGGCGGGTGGGCCCCTGGAGCCCATCCAGGCCATCATCCCCGGGG
GGTCCTCCACCCCGCCTCTGCCCTTCACCGAGGAGGTCCTGGACACCCCCATGAGCTACGAGCACCTCCAGGCCAAGGGC
TCCATGCTGGGGACGGGGGGCGTGATCCTCATCCCCGAGCGGGTGAGCATGGTGGACGCCATGTGGAACCTCACCCGCTT
CTACGCCCACGAGTCCTGCGGCAAGTGCACCCCCTGCCGCGAGGGCGTGGCGGGGTTCATGGTGAACCTCTTCGCCAAGA
TCGGCACCGGCCAGGGGGAGGAGAAGGACGTGGAGAACCTGGAAGCCCTCCTCCCCCTCATTGAGGGGCGGAGCTTCTGC
CCCTTGGCGGACGCGGCGGTGTGGCCGGTGAAGGGCTCGCTAAGGCACTTCAAGGACCAGTACCTGGCCCTGGCGCGGGA
GAAGCGCCCCGTGCCCAGGCCTTCCCTCTGGAGGTGA

Upstream 100 bases:

>100_bases
CGAGGCTCGAGGCCCTTCTTGCAGGCCTTAGGGCGGGGAAGCGGCTTGAGGAGATTGAGCTTCCCGGAAAGTGCGGCCAC
CACGTGCACGAGGTGGAGGT

Downstream 100 bases:

>100_bases
GATGGTCCGGGTCAAGGTGAACGACCGCATCGTGGAGGTGCCCCCGGGGACGAGCGTCATGGACGCCGTCTTCCACGCGG
GGTACGACGTGCCCCTCTTC

Product: NADH-quinone oxidoreductase subunit 1

Products: NA

Alternate protein names: NADH dehydrogenase I chain 1; NDH-1 subunit 1

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MTGPILSGLDPRFERTLYAHVGKEGSWTLDYYLRHGGYETAKRVLKEKTPDEVIEEVKRSGLRGRGGAGFPTGLKWSFMP
KDDGKQHYLICNADESEPGSFKDRYILEDVPHLLIEGMILAGYAIRATVGYIYVRGEYRRAADRLEQAIKEARARGYLGK
NLFGTDFSFDLHVHRGAGAYICGEETALMNSLEGLRANPRLKPPFPAQSGLWGKPTTINNVETLASVVPIMERGADWFAQ
MGTEQSKGMKLYQISGPVKRPGVYELPMGTTFRELIYEWAGGPLEPIQAIIPGGSSTPPLPFTEEVLDTPMSYEHLQAKG
SMLGTGGVILIPERVSMVDAMWNLTRFYAHESCGKCTPCREGVAGFMVNLFAKIGTGQGEEKDVENLEALLPLIEGRSFC
PLADAAVWPVKGSLRHFKDQYLALAREKRPVPRPSLWR

Sequences:

>Translated_438_residues
MTGPILSGLDPRFERTLYAHVGKEGSWTLDYYLRHGGYETAKRVLKEKTPDEVIEEVKRSGLRGRGGAGFPTGLKWSFMP
KDDGKQHYLICNADESEPGSFKDRYILEDVPHLLIEGMILAGYAIRATVGYIYVRGEYRRAADRLEQAIKEARARGYLGK
NLFGTDFSFDLHVHRGAGAYICGEETALMNSLEGLRANPRLKPPFPAQSGLWGKPTTINNVETLASVVPIMERGADWFAQ
MGTEQSKGMKLYQISGPVKRPGVYELPMGTTFRELIYEWAGGPLEPIQAIIPGGSSTPPLPFTEEVLDTPMSYEHLQAKG
SMLGTGGVILIPERVSMVDAMWNLTRFYAHESCGKCTPCREGVAGFMVNLFAKIGTGQGEEKDVENLEALLPLIEGRSFC
PLADAAVWPVKGSLRHFKDQYLALAREKRPVPRPSLWR
>Mature_437_residues
TGPILSGLDPRFERTLYAHVGKEGSWTLDYYLRHGGYETAKRVLKEKTPDEVIEEVKRSGLRGRGGAGFPTGLKWSFMPK
DDGKQHYLICNADESEPGSFKDRYILEDVPHLLIEGMILAGYAIRATVGYIYVRGEYRRAADRLEQAIKEARARGYLGKN
LFGTDFSFDLHVHRGAGAYICGEETALMNSLEGLRANPRLKPPFPAQSGLWGKPTTINNVETLASVVPIMERGADWFAQM
GTEQSKGMKLYQISGPVKRPGVYELPMGTTFRELIYEWAGGPLEPIQAIIPGGSSTPPLPFTEEVLDTPMSYEHLQAKGS
MLGTGGVILIPERVSMVDAMWNLTRFYAHESCGKCTPCREGVAGFMVNLFAKIGTGQGEEKDVENLEALLPLIEGRSFCP
LADAAVWPVKGSLRHFKDQYLALAREKRPVPRPSLWR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family

Homologues:

Organism=Homo sapiens, GI20149568, Length=411, Percent_Identity=46.9586374695864, Blast_Score=356, Evalue=2e-98,
Organism=Homo sapiens, GI260656005, Length=411, Percent_Identity=46.9586374695864, Blast_Score=356, Evalue=3e-98,
Organism=Escherichia coli, GI1788620, Length=405, Percent_Identity=49.1358024691358, Blast_Score=391, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17531727, Length=398, Percent_Identity=47.7386934673367, Blast_Score=359, Evalue=2e-99,
Organism=Drosophila melanogaster, GI19920794, Length=409, Percent_Identity=44.9877750611247, Blast_Score=358, Evalue=5e-99,
Organism=Drosophila melanogaster, GI45550452, Length=407, Percent_Identity=45.945945945946, Blast_Score=349, Evalue=2e-96,
Organism=Drosophila melanogaster, GI24653819, Length=400, Percent_Identity=35.25, Blast_Score=228, Evalue=8e-60,
Organism=Drosophila melanogaster, GI24653817, Length=351, Percent_Identity=34.1880341880342, Blast_Score=191, Evalue=6e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NQO1_THET8 (Q56222)

Other databases:

- EMBL:   U52917
- EMBL:   AP008226
- PIR:   T11903
- RefSeq:   YP_143355.1
- PDB:   2FUG
- PDB:   3I9V
- PDB:   3IAM
- PDB:   3IAS
- PDB:   3M9S
- PDBsum:   2FUG
- PDBsum:   3I9V
- PDBsum:   3IAM
- PDBsum:   3IAS
- PDBsum:   3M9S
- ProteinModelPortal:   Q56222
- SMR:   Q56222
- STRING:   Q56222
- GeneID:   3168453
- GenomeReviews:   AP008226_GR
- KEGG:   ttj:TTHA0089
- NMPDR:   fig|300852.3.peg.120
- eggNOG:   COG1894
- HOGENOM:   HBG669338
- OMA:   RELIYEW
- ProtClustDB:   CLSK445102
- BioCyc:   TTHE300852:TTHA0089-MONOMER
- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554
- SMART:   SM00928
- TIGRFAMs:   TIGR01959

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB

EC number: =1.6.99.5

Molecular weight: Translated: 48632; Mature: 48501

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS00644 COMPLEX1_51K_1; PS00645 COMPLEX1_51K_2

Important sites: BINDING 75-75 BINDING 97-97

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGPILSGLDPRFERTLYAHVGKEGSWTLDYYLRHGGYETAKRVLKEKTPDEVIEEVKRS
CCCCCCCCCCHHHHHHHHHHCCCCCCEEEEHHHHHCCHHHHHHHHHCCCHHHHHHHHHHC
GLRGRGGAGFPTGLKWSFMPKDDGKQHYLICNADESEPGSFKDRYILEDVPHLLIEGMIL
CCCCCCCCCCCCCCEEEECCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
AGYAIRATVGYIYVRGEYRRAADRLEQAIKEARARGYLGKNLFGTDFSFDLHVHRGAGAY
HHHHHEEEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCEE
ICGEETALMNSLEGLRANPRLKPPFPAQSGLWGKPTTINNVETLASVVPIMERGADWFAQ
EECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHH
MGTEQSKGMKLYQISGPVKRPGVYELPMGTTFRELIYEWAGGPLEPIQAIIPGGSSTPPL
CCCCHHCCEEEEEECCCCCCCCEEECCCCCHHHHHHHHHCCCCHHHHHHEECCCCCCCCC
PFTEEVLDTPMSYEHLQAKGSMLGTGGVILIPERVSMVDAMWNLTRFYAHESCGKCTPCR
CCCHHHHCCCCCHHHHHHCCCEECCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCHH
EGVAGFMVNLFAKIGTGQGEEKDVENLEALLPLIEGRSFCPLADAAVWPVKGSLRHFKDQ
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCHHHHHHHH
YLALAREKRPVPRPSLWR
HHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TGPILSGLDPRFERTLYAHVGKEGSWTLDYYLRHGGYETAKRVLKEKTPDEVIEEVKRS
CCCCCCCCCHHHHHHHHHHCCCCCCEEEEHHHHHCCHHHHHHHHHCCCHHHHHHHHHHC
GLRGRGGAGFPTGLKWSFMPKDDGKQHYLICNADESEPGSFKDRYILEDVPHLLIEGMIL
CCCCCCCCCCCCCCEEEECCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
AGYAIRATVGYIYVRGEYRRAADRLEQAIKEARARGYLGKNLFGTDFSFDLHVHRGAGAY
HHHHHEEEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCEE
ICGEETALMNSLEGLRANPRLKPPFPAQSGLWGKPTTINNVETLASVVPIMERGADWFAQ
EECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHH
MGTEQSKGMKLYQISGPVKRPGVYELPMGTTFRELIYEWAGGPLEPIQAIIPGGSSTPPL
CCCCHHCCEEEEEECCCCCCCCEEECCCCCHHHHHHHHHCCCCHHHHHHEECCCCCCCCC
PFTEEVLDTPMSYEHLQAKGSMLGTGGVILIPERVSMVDAMWNLTRFYAHESCGKCTPCR
CCCHHHHCCCCCHHHHHHCCCEECCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCHH
EGVAGFMVNLFAKIGTGQGEEKDVENLEALLPLIEGRSFCPLADAAVWPVKGSLRHFKDQ
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCHHHHHHHH
YLALAREKRPVPRPSLWR
HHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9020134