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The map label for this gene is prfC

Identifier: 54296894

GI number: 54296894

Start: 1039168

End: 1040748

Strand: Direct

Name: prfC

Synonym: lpp0935

Alternate gene names: 54296894

Gene position: 1039168-1040748 (Clockwise)

Preceding gene: 54296892

Following gene: 54296901

Centisome position: 29.66

GC content: 39.28

Gene sequence:

>1581_bases
ATGTCTGATTTTTATCAAGACTTTAACAAAAGGCGAACTTTTGCCATTATTTCTCACCCTGATGCAGGAAAGACCACTGT
AACTGAAAAGTTATTACTTTTTGGAGGAGCTATTCAGTTGGCAGGTACGGTCAAAGGCCGTAAAGCAGACAGGCATGCCA
CTTCTGATTGGATGGAAATGGAAAAAGAGAGAGGAATATCGATTACCACGTCTGTTATGCAGTTTATACATAATCAACAC
GTCATCAATTTGCTAGATACACCAGGCCATGAAGACTTTTCTGAAGATACATATCGTACTCTCACTGCTGTAGATTCTGC
CTTAATGGTAATTGACGTGGCCAAGGGAGTAGAGGAGCGAACGGTAAAATTGATGGAAGTTTGCCGTTTACGTGATACAC
CGATCATGACTTTTATCAATAAATTGGACAGAGAAGGGCGAGAACCTATTGATTTGTTAGATGAAGTAGAGTCTGTTTTG
GGCATTCAATGTGCGCCAATTACCTGGCCAGTTGGAATGGGTAAACGCTTCAAGGGAATTTATCACCGCTATCAAGATAT
TATTTATTTATATCAGCAGGGAAGTAATGCAAAAAAAGTCGAAGCTATGCAGATTAAAGGATTGGATAATCCTCAATTAG
ATGAGTTGATAGGTGACAGTGCGGACGAACTCAGAGAAGAAATTGAATTAGTCAAAGGAGCGTCCCACGAGTTTAACTTG
GAAGCTTATCTTGCAGGAAAAATGACCCCGGTATACTTTGGTTCAGCAATTAATAATTTTGGTATTAAAGAATTATTGGA
CGATTTTGTCGAGTATGCGCCAGGACCTCAACCTCGAGCAACTCAGGAGAGAGTTGTTTCTCCGCATGAAGAAACATTTT
CCGGTTTTGTTTTTAAAATTCAAGCCAATATGGATCCCAAACACAGAGACCGAATTGCCTTTGTGCGGGTTTGTTCTGGC
TCTTACAAGAAGGGAATGAAATTAAATCATTTACGCATAGGAAAAGAAGTGCAAATTTCCAATGCCTTAACTTTTATGGC
AGGCGATAGATCACACACAGAACTTGCTTTAGCTGGTGATATTATTGGTTTGCATAATCATGGTACTATTCGTATTGGTG
ATACTTTTACACAAGGTGAACACTTAAAGTTTACTGGAATTCCTAATTTTGCTCCTGAATTGTTTAGGCTTGTCAGGTTG
AGGGACCCATTAAAGAGTAAAGCCTTACTTAAAGGATTAATCGAATTATCTGAAGAGGGTGCTACCCAGGTTTTTAGACC
ATTAAACAGTAATCAGCTAATATTGGGAGCTGTTGGGATATTACAATTTGATGTGGTAGCGCATAGACTGAAACATGAAT
ATAAAGTGGATTGTATTTATGAATCAGTCAATATAGCTTGCGCTCGATGGGTTTATTCTGAAGATGATAAAGCAATGAGC
GAGTTTCGTACCAAGGCTTATGATTATTTGGCTTTGGACGGAGGAGATATGCTTATGTACTTGGCGCCTACCAAAGTGAA
TTTGACAATGGCAGAAGAACGTTATCCCAAGATTAAGTTCTGCGCTACTCGAGAACATTGA

Upstream 100 bases:

>100_bases
AATGATTTTAATTCGCTACGTTTATGGGGTCGTAAGTTCCATGGTATACTATGCGATTGAAGCCTATTATTTTTAATTAA
TTACTTAATTTGAGAGCAGT

Downstream 100 bases:

>100_bases
TGAACAGGATTTGAATTTTCACACCCCATTACTGTTTGGTGTTGGGGTGCGATTAGCTCAATTCTTTATGCAGTACAAGT
TGCTTTGAGAGTATGTGGTC

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSDFYQDFNKRRTFAIISHPDAGKTTVTEKLLLFGGAIQLAGTVKGRKADRHATSDWMEMEKERGISITTSVMQFIHNQH
VINLLDTPGHEDFSEDTYRTLTAVDSALMVIDVAKGVEERTVKLMEVCRLRDTPIMTFINKLDREGREPIDLLDEVESVL
GIQCAPITWPVGMGKRFKGIYHRYQDIIYLYQQGSNAKKVEAMQIKGLDNPQLDELIGDSADELREEIELVKGASHEFNL
EAYLAGKMTPVYFGSAINNFGIKELLDDFVEYAPGPQPRATQERVVSPHEETFSGFVFKIQANMDPKHRDRIAFVRVCSG
SYKKGMKLNHLRIGKEVQISNALTFMAGDRSHTELALAGDIIGLHNHGTIRIGDTFTQGEHLKFTGIPNFAPELFRLVRL
RDPLKSKALLKGLIELSEEGATQVFRPLNSNQLILGAVGILQFDVVAHRLKHEYKVDCIYESVNIACARWVYSEDDKAMS
EFRTKAYDYLALDGGDMLMYLAPTKVNLTMAEERYPKIKFCATREH

Sequences:

>Translated_526_residues
MSDFYQDFNKRRTFAIISHPDAGKTTVTEKLLLFGGAIQLAGTVKGRKADRHATSDWMEMEKERGISITTSVMQFIHNQH
VINLLDTPGHEDFSEDTYRTLTAVDSALMVIDVAKGVEERTVKLMEVCRLRDTPIMTFINKLDREGREPIDLLDEVESVL
GIQCAPITWPVGMGKRFKGIYHRYQDIIYLYQQGSNAKKVEAMQIKGLDNPQLDELIGDSADELREEIELVKGASHEFNL
EAYLAGKMTPVYFGSAINNFGIKELLDDFVEYAPGPQPRATQERVVSPHEETFSGFVFKIQANMDPKHRDRIAFVRVCSG
SYKKGMKLNHLRIGKEVQISNALTFMAGDRSHTELALAGDIIGLHNHGTIRIGDTFTQGEHLKFTGIPNFAPELFRLVRL
RDPLKSKALLKGLIELSEEGATQVFRPLNSNQLILGAVGILQFDVVAHRLKHEYKVDCIYESVNIACARWVYSEDDKAMS
EFRTKAYDYLALDGGDMLMYLAPTKVNLTMAEERYPKIKFCATREH
>Mature_525_residues
SDFYQDFNKRRTFAIISHPDAGKTTVTEKLLLFGGAIQLAGTVKGRKADRHATSDWMEMEKERGISITTSVMQFIHNQHV
INLLDTPGHEDFSEDTYRTLTAVDSALMVIDVAKGVEERTVKLMEVCRLRDTPIMTFINKLDREGREPIDLLDEVESVLG
IQCAPITWPVGMGKRFKGIYHRYQDIIYLYQQGSNAKKVEAMQIKGLDNPQLDELIGDSADELREEIELVKGASHEFNLE
AYLAGKMTPVYFGSAINNFGIKELLDDFVEYAPGPQPRATQERVVSPHEETFSGFVFKIQANMDPKHRDRIAFVRVCSGS
YKKGMKLNHLRIGKEVQISNALTFMAGDRSHTELALAGDIIGLHNHGTIRIGDTFTQGEHLKFTGIPNFAPELFRLVRLR
DPLKSKALLKGLIELSEEGATQVFRPLNSNQLILGAVGILQFDVVAHRLKHEYKVDCIYESVNIACARWVYSEDDKAMSE
FRTKAYDYLALDGGDMLMYLAPTKVNLTMAEERYPKIKFCATREH

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily

Homologues:

Organism=Homo sapiens, GI18390331, Length=494, Percent_Identity=28.3400809716599, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI19923640, Length=507, Percent_Identity=25.4437869822485, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI25306283, Length=391, Percent_Identity=27.6214833759591, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI25306287, Length=291, Percent_Identity=29.553264604811, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI157426893, Length=168, Percent_Identity=30.952380952381, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI94966754, Length=137, Percent_Identity=29.9270072992701, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1790835, Length=527, Percent_Identity=67.3624288425047, Blast_Score=746, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=490, Percent_Identity=27.1428571428571, Blast_Score=148, Evalue=9e-37,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=32.0512820512821, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI48994988, Length=147, Percent_Identity=36.734693877551, Blast_Score=89, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=492, Percent_Identity=28.0487804878049, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17556745, Length=179, Percent_Identity=35.7541899441341, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17557151, Length=146, Percent_Identity=32.8767123287671, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17506493, Length=162, Percent_Identity=30.2469135802469, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=30.8270676691729, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=30.8270676691729, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=480, Percent_Identity=27.7083333333333, Blast_Score=173, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6322359, Length=543, Percent_Identity=24.1252302025783, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6324707, Length=150, Percent_Identity=32.6666666666667, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320593, Length=150, Percent_Identity=32.6666666666667, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323320, Length=138, Percent_Identity=27.536231884058, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24582462, Length=472, Percent_Identity=27.7542372881356, Blast_Score=161, Evalue=8e-40,
Organism=Drosophila melanogaster, GI221458488, Length=520, Percent_Identity=26.5384615384615, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI78706572, Length=155, Percent_Identity=31.6129032258064, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24585711, Length=154, Percent_Identity=32.4675324675325, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24585713, Length=154, Percent_Identity=32.4675324675325, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24585709, Length=154, Percent_Identity=32.4675324675325, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF3_LEGPA (Q5X6N0)

Other databases:

- EMBL:   CR628336
- RefSeq:   YP_123263.1
- ProteinModelPortal:   Q5X6N0
- SMR:   Q5X6N0
- STRING:   Q5X6N0
- GeneID:   3118941
- GenomeReviews:   CR628336_GR
- KEGG:   lpp:lpp0935
- LegioList:   lpp0935
- eggNOG:   COG4108
- HOGENOM:   HBG285678
- OMA:   LQFEVVQ
- ProtClustDB:   PRK00741
- BioCyc:   LPNE297246:LPP0935-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00072
- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00503
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 59477; Mature: 59346

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDFYQDFNKRRTFAIISHPDAGKTTVTEKLLLFGGAIQLAGTVKGRKADRHATSDWMEM
CCHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHH
EKERGISITTSVMQFIHNQHVINLLDTPGHEDFSEDTYRTLTAVDSALMVIDVAKGVEER
HHHCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHH
TVKLMEVCRLRDTPIMTFINKLDREGREPIDLLDEVESVLGIQCAPITWPVGMGKRFKGI
HHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEECCEEECCCCCHHHHHH
YHRYQDIIYLYQQGSNAKKVEAMQIKGLDNPQLDELIGDSADELREEIELVKGASHEFNL
HHHHHHHEEEEECCCCCCEEEEEEECCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCE
EAYLAGKMTPVYFGSAINNFGIKELLDDFVEYAPGPQPRATQERVVSPHEETFSGFVFKI
EEEEECCCCCEEECCHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHCCCCEEEEE
QANMDPKHRDRIAFVRVCSGSYKKGMKLNHLRIGKEVQISNALTFMAGDRSHTELALAGD
ECCCCCCHHCCEEEEEEECCCCCCCCEEEEEECCCEEEECCEEEEEECCCCCCEEEEECC
IIGLHNHGTIRIGDTFTQGEHLKFTGIPNFAPELFRLVRLRDPLKSKALLKGLIELSEEG
EEEECCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
ATQVFRPLNSNQLILGAVGILQFDVVAHRLKHEYKVDCIYESVNIACARWVYSEDDKAMS
HHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEEEECCCHHHHH
EFRTKAYDYLALDGGDMLMYLAPTKVNLTMAEERYPKIKFCATREH
HHHHHHEEEEEECCCCEEEEEECCEEEEEEHHCCCCCEEEEECCCC
>Mature Secondary Structure 
SDFYQDFNKRRTFAIISHPDAGKTTVTEKLLLFGGAIQLAGTVKGRKADRHATSDWMEM
CHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHH
EKERGISITTSVMQFIHNQHVINLLDTPGHEDFSEDTYRTLTAVDSALMVIDVAKGVEER
HHHCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHH
TVKLMEVCRLRDTPIMTFINKLDREGREPIDLLDEVESVLGIQCAPITWPVGMGKRFKGI
HHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEECCEEECCCCCHHHHHH
YHRYQDIIYLYQQGSNAKKVEAMQIKGLDNPQLDELIGDSADELREEIELVKGASHEFNL
HHHHHHHEEEEECCCCCCEEEEEEECCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCE
EAYLAGKMTPVYFGSAINNFGIKELLDDFVEYAPGPQPRATQERVVSPHEETFSGFVFKI
EEEEECCCCCEEECCHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHCCCCEEEEE
QANMDPKHRDRIAFVRVCSGSYKKGMKLNHLRIGKEVQISNALTFMAGDRSHTELALAGD
ECCCCCCHHCCEEEEEEECCCCCCCCEEEEEECCCEEEECCEEEEEECCCCCCEEEEECC
IIGLHNHGTIRIGDTFTQGEHLKFTGIPNFAPELFRLVRLRDPLKSKALLKGLIELSEEG
EEEECCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
ATQVFRPLNSNQLILGAVGILQFDVVAHRLKHEYKVDCIYESVNIACARWVYSEDDKAMS
HHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEEEECCCHHHHH
EFRTKAYDYLALDGGDMLMYLAPTKVNLTMAEERYPKIKFCATREH
HHHHHHEEEEEECCCCEEEEEECCEEEEEEHHCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA