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The map label for this gene is lspF [H]

Identifier: 54294249

GI number: 54294249

Start: 1469409

End: 1470608

Strand: Reverse

Name: lspF [H]

Synonym: lpl1314

Alternate gene names: 54294249

Gene position: 1470608-1469409 (Counterclockwise)

Preceding gene: 54294256

Following gene: 54294248

Centisome position: 43.96

GC content: 40.33

Gene sequence:

>1200_bases
ATGGGCGCCTACCAATATCAAGCGCTAAAAGTGAATGGCAGCATTAGCAAAGGTGTAATAGAAGCTGATTCTGAACGTCA
TGCACGTCAATTATTGCGAGAACAAGGACTAATCCCTACTCAAGTCCAAACATTAACCCAGCAACGTGCTGCCAGCAGCA
AAGGTAAAGTTTCTGCGGCTGATTTGGCATTACTCACGAGACAGCTGGCAACACTTCTGGCTGCCGGTATTCCTGTTGAA
GAATCATTGCGCGGGGTCAGCGAACAAACAGAAAAAGACAAAGTCAGAGAGCTTATTATCGGAGTTCGCTCTAAAGTATT
GGAAGGGTATGGTCTTGCTCAGGCAATGGCTCAATATCCCAATGCCTTTCCAGAACTATACCGGGCAACAGTTGGCTCAG
GCGAGCAGACAGGGCGCCTTGATGTCGTTTTAGAAAAATTAGCTGATTATACTGAAAAACAACAACAAACTCGACAAAAA
GTGCAGCAAGCCTTGATTTACCCATTGCTCATGATCATTGTTTCTACTGCCATTATCAGTTTTTTACTTACTTTTGTCGT
CCCTAAAATTATTGATGTGTTTACCGAAAGTGGCCAAACACTACCACCAATGACGCAATTGCTTATTAACATGAGCCAAT
TTATCAAATCTTATGGTGTGTATAGTCTAGTGACAATAATTGTGGCTCTGATTGGATTTAAAAAAAGCCTCAATAATATA
AAAATTAAAACAGCATGGCATCAATTCATGCTTAAACTACCTATCGTGTCTTATTTGGTTAAAACAATTAATGTAGCACG
ATATATTCACACGTTTGGAATTCTATTTGCAGCGGGAGTCAGTGTACTTGAAACCATGCGTGTTTCTTCAAGTTTGGTAA
CCAATATCGTGATGAGACAGGCTTTTGATTTAGCTACTCTACGAGTCCGTGAAGGAAGTGGAATCAGTGAAGCCCTCAAA
GAAACAAAATTCATTAGCCCTATGGCTATCCATCTCATAGCCAGTGGTGAGAAAAGCGGCCAACTTTCAGACATGATGGA
ACGCTCGGCGTCTCATTTAGATAATGAAGTAAACCGACTGATTGAAACATCCTTAACTTTATTAGAACCTATGGTTATTC
TATTAATGGGTGCTGTTGTTTTGTTTATTGTACTTGCGACCTTATTACCTATTTTTTCAATGGAACAATTAGTTGCCTGA

Upstream 100 bases:

>100_bases
TCATCTTTTGTCTCCAGGATAGTCCGGGTATAGTTTACCTTAAGTGACTTATTAATCCCAATAGATTACACTCTATAGTT
TTAATTTTAACAAAAATAAT

Downstream 100 bases:

>100_bases
TTAAGCAGAGGAAGGGTTATCCGCACCCTTTTAAAATTTAAGTCTATTCATAGGTATCAATATTTGTGTTATAATTTAAC
ATTTAGTTTCAAGAGGTACA

Product: type II secretory pathway protein LspF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MGAYQYQALKVNGSISKGVIEADSERHARQLLREQGLIPTQVQTLTQQRAASSKGKVSAADLALLTRQLATLLAAGIPVE
ESLRGVSEQTEKDKVRELIIGVRSKVLEGYGLAQAMAQYPNAFPELYRATVGSGEQTGRLDVVLEKLADYTEKQQQTRQK
VQQALIYPLLMIIVSTAIISFLLTFVVPKIIDVFTESGQTLPPMTQLLINMSQFIKSYGVYSLVTIIVALIGFKKSLNNI
KIKTAWHQFMLKLPIVSYLVKTINVARYIHTFGILFAAGVSVLETMRVSSSLVTNIVMRQAFDLATLRVREGSGISEALK
ETKFISPMAIHLIASGEKSGQLSDMMERSASHLDNEVNRLIETSLTLLEPMVILLMGAVVLFIVLATLLPIFSMEQLVA

Sequences:

>Translated_399_residues
MGAYQYQALKVNGSISKGVIEADSERHARQLLREQGLIPTQVQTLTQQRAASSKGKVSAADLALLTRQLATLLAAGIPVE
ESLRGVSEQTEKDKVRELIIGVRSKVLEGYGLAQAMAQYPNAFPELYRATVGSGEQTGRLDVVLEKLADYTEKQQQTRQK
VQQALIYPLLMIIVSTAIISFLLTFVVPKIIDVFTESGQTLPPMTQLLINMSQFIKSYGVYSLVTIIVALIGFKKSLNNI
KIKTAWHQFMLKLPIVSYLVKTINVARYIHTFGILFAAGVSVLETMRVSSSLVTNIVMRQAFDLATLRVREGSGISEALK
ETKFISPMAIHLIASGEKSGQLSDMMERSASHLDNEVNRLIETSLTLLEPMVILLMGAVVLFIVLATLLPIFSMEQLVA
>Mature_398_residues
GAYQYQALKVNGSISKGVIEADSERHARQLLREQGLIPTQVQTLTQQRAASSKGKVSAADLALLTRQLATLLAAGIPVEE
SLRGVSEQTEKDKVRELIIGVRSKVLEGYGLAQAMAQYPNAFPELYRATVGSGEQTGRLDVVLEKLADYTEKQQQTRQKV
QQALIYPLLMIIVSTAIISFLLTFVVPKIIDVFTESGQTLPPMTQLLINMSQFIKSYGVYSLVTIIVALIGFKKSLNNIK
IKTAWHQFMLKLPIVSYLVKTINVARYIHTFGILFAAGVSVLETMRVSSSLVTNIVMRQAFDLATLRVREGSGISEALKE
TKFISPMAIHLIASGEKSGQLSDMMERSASHLDNEVNRLIETSLTLLEPMVILLMGAVVLFIVLATLLPIFSMEQLVA

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of a variety of enzymes across the outer membrane [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=395, Percent_Identity=36.4556962025316, Blast_Score=275, Evalue=4e-75,
Organism=Escherichia coli, GI1786295, Length=406, Percent_Identity=26.6009852216749, Blast_Score=132, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR011850
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 43942; Mature: 43811

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS00874 T2SP_F

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAYQYQALKVNGSISKGVIEADSERHARQLLREQGLIPTQVQTLTQQRAASSKGKVSAA
CCCCEEEEEEECCCHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHH
DLALLTRQLATLLAAGIPVEESLRGVSEQTEKDKVRELIIGVRSKVLEGYGLAQAMAQYP
HHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCC
NAFPELYRATVGSGEQTGRLDVVLEKLADYTEKQQQTRQKVQQALIYPLLMIIVSTAIIS
CHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLLTFVVPKIIDVFTESGQTLPPMTQLLINMSQFIKSYGVYSLVTIIVALIGFKKSLNNI
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KIKTAWHQFMLKLPIVSYLVKTINVARYIHTFGILFAAGVSVLETMRVSSSLVTNIVMRQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFDLATLRVREGSGISEALKETKFISPMAIHLIASGEKSGQLSDMMERSASHLDNEVNRL
HHHHHHHEEECCCCHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
IETSLTLLEPMVILLMGAVVLFIVLATLLPIFSMEQLVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GAYQYQALKVNGSISKGVIEADSERHARQLLREQGLIPTQVQTLTQQRAASSKGKVSAA
CCCEEEEEEECCCHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHH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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1588814; 10984043 [H]