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The map label for this gene is citH [H]

Identifier: 54294107

GI number: 54294107

Start: 1305117

End: 1306403

Strand: Direct

Name: citH [H]

Synonym: lpl1171

Alternate gene names: 54294107

Gene position: 1305117-1306403 (Clockwise)

Preceding gene: 54294105

Following gene: 54294108

Centisome position: 39.01

GC content: 39.24

Gene sequence:

>1287_bases
ATGAGTACATCAAATACCTCCTTTAAAGCATTGGGAGCAATATGTATTGGTAATGCCATGGAATGGTATGATTTCATGAT
TTATAACTTCATGCTGCTTTTTATTGCCCAAGCTTTTTTTCCCAATCATAGTCCTGTGACTTCTCTCTTGGCTACAATGG
CAAGCTCCGGAGTGGCTCTGGTAGTCCGGCCTTTCGGTGGTTTTATTTTAGCAGTTTGGGCAGATAAATATGGGCATCAT
AAAGCATTAATGTTCGTTTTTTATTTGATGGCTGTCGCAACCATGCTTATTACCTTTACACCATCCTACTCCACAATAGG
TCTGGCTGCTACTTGTGTCATTGTATTTGCAAGATTGTTGCAAGGGCTTGCTACTGGTGGCGAGTTTGGCATATCCAGCG
CTCTATTAGTTAGCTTATCGCCAAGCGATAAAAAGGGGTTTTACACTTCCTTACAAATGGTAGGCCAGCTTTTGGCAATA
TTAATGGGATCTTCAGCGTGCCTTATTTTAACTCTCTATTGTTCCAATGAAACCCTATATCAATTTGCCTGGAGAATACC
TTTTGCTTTAGGACTGTTGATTCTACCTTTAGGATGGACTTTAAGACGTCGATTGCAACGGGAATTTACCTGGAAGAAAG
AGCAACAGGACACAGGAAAGCTGTTTATCTTGGTAAAGCAACAAAAACGTTCGCTGTTGATAGCTTTTAGTCTTGTAGCC
GGGTGTACAGGCTCGATTTATACCTTATTTAGCTATATGCCTACCTATTGTAAATTGTACTTGAATTTATCGCTGACAGA
GGCGTACTTAGGCACTGCATCTGGAATATTAGTCAGTATTCTTACTATTCCCTTTTTTGGAGCATTGTCGGATAAAATAG
GCAAAAAAATAGTTATGTTATGTGCTTTGGCTTGTTATTTTGGATTAATTTATCCTTTACTTTTCTTGCTCAACCAATAC
CCTGGTGTAACTATTCTCATTTTGGTTGAAAACATTTTAGGTTTGTGTATCGGCGCTTATTTTGGAGTGTTGACCATTAC
GGTTAGCTCCTTATTTCCACCATCAGTACGTTCTACTTGTCTAGCCGTCAGCTATAATTCAGCTGTCCTGTTGTTTGGTG
GTTTTGCTCCATTTATTATAACGGCGTTGATTGAATACACTAAAAACCCTATGGTTTTTACTTATTATCAAATGACGACG
GTCTTTATCAGTTTGCTAGCAGTCCTTGCTTATCAGGAGGAAAGGAAGCTATCTGAGAATCAGCCAGAACATCTCGTTGC
AATTTAA

Upstream 100 bases:

>100_bases
AGTAAAATTAATATTGATATTAGTTTCTTATAAAGCATGGTAATTTTTTTTATATTCTACTAGTATGTGTTTTGCTCTAA
ATTTACCCTTGTAAGGAAAC

Downstream 100 bases:

>100_bases
AAGGTAGGAATATGACAACTTTAGATTGGTTAAAACAACTCATTTCTTTTGATACTACTTCGCGGAACTCTAATTTATCA
TTGATTGAATATTTGGCGAA

Product: hypothetical protein

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSTSNTSFKALGAICIGNAMEWYDFMIYNFMLLFIAQAFFPNHSPVTSLLATMASSGVALVVRPFGGFILAVWADKYGHH
KALMFVFYLMAVATMLITFTPSYSTIGLAATCVIVFARLLQGLATGGEFGISSALLVSLSPSDKKGFYTSLQMVGQLLAI
LMGSSACLILTLYCSNETLYQFAWRIPFALGLLILPLGWTLRRRLQREFTWKKEQQDTGKLFILVKQQKRSLLIAFSLVA
GCTGSIYTLFSYMPTYCKLYLNLSLTEAYLGTASGILVSILTIPFFGALSDKIGKKIVMLCALACYFGLIYPLLFLLNQY
PGVTILILVENILGLCIGAYFGVLTITVSSLFPPSVRSTCLAVSYNSAVLLFGGFAPFIITALIEYTKNPMVFTYYQMTT
VFISLLAVLAYQEERKLSENQPEHLVAI

Sequences:

>Translated_428_residues
MSTSNTSFKALGAICIGNAMEWYDFMIYNFMLLFIAQAFFPNHSPVTSLLATMASSGVALVVRPFGGFILAVWADKYGHH
KALMFVFYLMAVATMLITFTPSYSTIGLAATCVIVFARLLQGLATGGEFGISSALLVSLSPSDKKGFYTSLQMVGQLLAI
LMGSSACLILTLYCSNETLYQFAWRIPFALGLLILPLGWTLRRRLQREFTWKKEQQDTGKLFILVKQQKRSLLIAFSLVA
GCTGSIYTLFSYMPTYCKLYLNLSLTEAYLGTASGILVSILTIPFFGALSDKIGKKIVMLCALACYFGLIYPLLFLLNQY
PGVTILILVENILGLCIGAYFGVLTITVSSLFPPSVRSTCLAVSYNSAVLLFGGFAPFIITALIEYTKNPMVFTYYQMTT
VFISLLAVLAYQEERKLSENQPEHLVAI
>Mature_427_residues
STSNTSFKALGAICIGNAMEWYDFMIYNFMLLFIAQAFFPNHSPVTSLLATMASSGVALVVRPFGGFILAVWADKYGHHK
ALMFVFYLMAVATMLITFTPSYSTIGLAATCVIVFARLLQGLATGGEFGISSALLVSLSPSDKKGFYTSLQMVGQLLAIL
MGSSACLILTLYCSNETLYQFAWRIPFALGLLILPLGWTLRRRLQREFTWKKEQQDTGKLFILVKQQKRSLLIAFSLVAG
CTGSIYTLFSYMPTYCKLYLNLSLTEAYLGTASGILVSILTIPFFGALSDKIGKKIVMLCALACYFGLIYPLLFLLNQYP
GVTILILVENILGLCIGAYFGVLTITVSSLFPPSVRSTCLAVSYNSAVLLFGGFAPFIITALIEYTKNPMVFTYYQMTTV
FISLLAVLAYQEERKLSENQPEHLVAI

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=433, Percent_Identity=28.8683602771363, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1788942, Length=412, Percent_Identity=29.3689320388349, Blast_Score=161, Evalue=6e-41,
Organism=Escherichia coli, GI1788292, Length=432, Percent_Identity=28.9351851851852, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1789941, Length=433, Percent_Identity=26.7898383371824, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI87082404, Length=381, Percent_Identity=25.4593175853018, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47189; Mature: 47058

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSNTSFKALGAICIGNAMEWYDFMIYNFMLLFIAQAFFPNHSPVTSLLATMASSGVAL
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEE
VVRPFGGFILAVWADKYGHHKALMFVFYLMAVATMLITFTPSYSTIGLAATCVIVFARLL
EEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
QGLATGGEFGISSALLVSLSPSDKKGFYTSLQMVGQLLAILMGSSACLILTLYCSNETLY
HHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHH
QFAWRIPFALGLLILPLGWTLRRRLQREFTWKKEQQDTGKLFILVKQQKRSLLIAFSLVA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHH
GCTGSIYTLFSYMPTYCKLYLNLSLTEAYLGTASGILVSILTIPFFGALSDKIGKKIVML
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CALACYFGLIYPLLFLLNQYPGVTILILVENILGLCIGAYFGVLTITVSSLFPPSVRSTC
HHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHE
LAVSYNSAVLLFGGFAPFIITALIEYTKNPMVFTYYQMTTVFISLLAVLAYQEERKLSEN
EEEEECCEEEEECCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCC
QPEHLVAI
CCCCCCCC
>Mature Secondary Structure 
STSNTSFKALGAICIGNAMEWYDFMIYNFMLLFIAQAFFPNHSPVTSLLATMASSGVAL
CCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEE
VVRPFGGFILAVWADKYGHHKALMFVFYLMAVATMLITFTPSYSTIGLAATCVIVFARLL
EEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
QGLATGGEFGISSALLVSLSPSDKKGFYTSLQMVGQLLAILMGSSACLILTLYCSNETLY
HHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHH
QFAWRIPFALGLLILPLGWTLRRRLQREFTWKKEQQDTGKLFILVKQQKRSLLIAFSLVA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHH
GCTGSIYTLFSYMPTYCKLYLNLSLTEAYLGTASGILVSILTIPFFGALSDKIGKKIVML
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CALACYFGLIYPLLFLLNQYPGVTILILVENILGLCIGAYFGVLTITVSSLFPPSVRSTC
HHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHE
LAVSYNSAVLLFGGFAPFIITALIEYTKNPMVFTYYQMTTVFISLLAVLAYQEERKLSEN
EEEEECCEEEEECCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCC
QPEHLVAI
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]