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The map label for this gene is prfC [H]

Identifier: 54293849

GI number: 54293849

Start: 1016701

End: 1018281

Strand: Direct

Name: prfC [H]

Synonym: lpl0905

Alternate gene names: 54293849

Gene position: 1016701-1018281 (Clockwise)

Preceding gene: 54293846

Following gene: 54293856

Centisome position: 30.39

GC content: 39.91

Gene sequence:

>1581_bases
ATGTCTGATTTTTATCAAGACTTTAATAAAAGGCGAACTTTTGCCATTATTTCTCACCCTGATGCAGGAAAGACCACTGT
AACTGAAAAATTATTACTTTTCGGAGGAGCTATTCAGCTGGCAGGTACGGTTAAAGGCCGTAAAGCAGACAGGCATGCCA
CTTCTGATTGGATGGAAATGGAAAAAGAGAGAGGAATATCGATTACTACGTCCGTTATGCAGTTTGTACATAATCAACAC
GTCATCAATTTGCTTGATACACCAGGCCATGAAGACTTTTCTGAAGACACCTATCGTACTCTTACAGCTGTAGATTCTGC
CTTAATGGTAATTGACGTGGCCAAGGGAGTAGAGGAGCGAACGGTAAAATTGATGGAAGTTTGCCGTTTACGTGATACAC
CGATCATGACTTTTATCAATAAATTGGACAGGGAAGGGCGAGAGCCTATTGATTTGCTCGATGAAGTAGAGTCTGTTTTG
GGCATTCAATGTGCCCCAATTACCTGGCCAGTTGGAATGGGAAAACGCTTCAAAGGAATTTATCATCGCTATCAGGATAT
TATTTATTTATATCAACAAGGAAGTAATGCAAAAAAAATAGATGCTACGCAGATTAAAGGTTTGGATAATCCTCAATTAG
ATGAGTTGATAGGTGACAGCGCAAAAGAACTCAGAGAAGAAATTGAATTAGTCAGGGGGGCATCCAACGAGTTTAATTTG
CAAGATTACCTTGCAGGCAAAATGACTCCCGTGTATTTCGGTTCAGCAATTAATAATTTTGGCATTAAAGAATTATTGGA
TGATTTTGTCGAGTATGCGCCGGGTCCTCAACCGCGAGCAACTCAGGAGAGAGTTGTTTCTCCGAATGAAGAAACATTTT
CCGGTTTTGTTTTTAAAATTCAAGCCAATATGGATCCCAAACACAGAGACCGAATTGCCTTTGTGCGGGTTTGTTCAGGC
TCTTACAAGAAGGGAATGAAATTAAGTCATTTACGCATAGGAAAAGAAGTGCAAATTTCCAATGCCTTAACTTTTATGGC
AGGCGATAGATCGCACACAGAACTTGCTTTAGCTGGTGATATTATTGGTTTGCATAATCATGGTACTATTCGTATTGGTG
ATACTTTTACACAGGGTGAACAATTAAAGTTTACTGGAATTCCTAATTTTGCTCCTGAATTGTTTAGGCTTGTCAGGTTG
AGGGACCCATTAAAGAGTAAAGCCTTGCTTAAAGGATTAATCGAATTATCTGAAGAGGGTGCTACCCAGGTTTTTAGGCC
ATTAAACAGTAATCAGCTAATATTGGGAGCTGTTGGGATATTACAATTTGATGTGGTAGCGCATAGACTGAAATATGAAT
ATAAAGTGGATTGTATTTATGAATCAGTCAATATAGCTTGCGCTCGATGGGTTTATTCTGACGATGATAAAGCAATGAGC
GAGTTTCGTACCAAGGCTTATGATTATTTGGCTCTGGATGGAGGAGATATGCTTATGTACCTGGCGCCTACCAAAGTGAA
TTTGACAATGGCAGAGGAGCGTTATCCGAAGATTAAGTTCTGCGCTACCCGCGAACATTGA

Upstream 100 bases:

>100_bases
GATAAACAACAGCGAGACTTTAATTTATAACGAAAAAATTGTTAGTTTTGTACTTTTCCTTTACAATTCACAGCAATTAA
TTACTTACCTAGAGATTCAC

Downstream 100 bases:

>100_bases
TGAACAGGATTTGAATGTTCGCACCCCATTACTGTTCAGTGTTGGGGTGCGGTTAGCTCAATTCTTTATGCGGTACAAGT
TGCTTTGAGAGTATGTGGTC

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSDFYQDFNKRRTFAIISHPDAGKTTVTEKLLLFGGAIQLAGTVKGRKADRHATSDWMEMEKERGISITTSVMQFVHNQH
VINLLDTPGHEDFSEDTYRTLTAVDSALMVIDVAKGVEERTVKLMEVCRLRDTPIMTFINKLDREGREPIDLLDEVESVL
GIQCAPITWPVGMGKRFKGIYHRYQDIIYLYQQGSNAKKIDATQIKGLDNPQLDELIGDSAKELREEIELVRGASNEFNL
QDYLAGKMTPVYFGSAINNFGIKELLDDFVEYAPGPQPRATQERVVSPNEETFSGFVFKIQANMDPKHRDRIAFVRVCSG
SYKKGMKLSHLRIGKEVQISNALTFMAGDRSHTELALAGDIIGLHNHGTIRIGDTFTQGEQLKFTGIPNFAPELFRLVRL
RDPLKSKALLKGLIELSEEGATQVFRPLNSNQLILGAVGILQFDVVAHRLKYEYKVDCIYESVNIACARWVYSDDDKAMS
EFRTKAYDYLALDGGDMLMYLAPTKVNLTMAEERYPKIKFCATREH

Sequences:

>Translated_526_residues
MSDFYQDFNKRRTFAIISHPDAGKTTVTEKLLLFGGAIQLAGTVKGRKADRHATSDWMEMEKERGISITTSVMQFVHNQH
VINLLDTPGHEDFSEDTYRTLTAVDSALMVIDVAKGVEERTVKLMEVCRLRDTPIMTFINKLDREGREPIDLLDEVESVL
GIQCAPITWPVGMGKRFKGIYHRYQDIIYLYQQGSNAKKIDATQIKGLDNPQLDELIGDSAKELREEIELVRGASNEFNL
QDYLAGKMTPVYFGSAINNFGIKELLDDFVEYAPGPQPRATQERVVSPNEETFSGFVFKIQANMDPKHRDRIAFVRVCSG
SYKKGMKLSHLRIGKEVQISNALTFMAGDRSHTELALAGDIIGLHNHGTIRIGDTFTQGEQLKFTGIPNFAPELFRLVRL
RDPLKSKALLKGLIELSEEGATQVFRPLNSNQLILGAVGILQFDVVAHRLKYEYKVDCIYESVNIACARWVYSDDDKAMS
EFRTKAYDYLALDGGDMLMYLAPTKVNLTMAEERYPKIKFCATREH
>Mature_525_residues
SDFYQDFNKRRTFAIISHPDAGKTTVTEKLLLFGGAIQLAGTVKGRKADRHATSDWMEMEKERGISITTSVMQFVHNQHV
INLLDTPGHEDFSEDTYRTLTAVDSALMVIDVAKGVEERTVKLMEVCRLRDTPIMTFINKLDREGREPIDLLDEVESVLG
IQCAPITWPVGMGKRFKGIYHRYQDIIYLYQQGSNAKKIDATQIKGLDNPQLDELIGDSAKELREEIELVRGASNEFNLQ
DYLAGKMTPVYFGSAINNFGIKELLDDFVEYAPGPQPRATQERVVSPNEETFSGFVFKIQANMDPKHRDRIAFVRVCSGS
YKKGMKLSHLRIGKEVQISNALTFMAGDRSHTELALAGDIIGLHNHGTIRIGDTFTQGEQLKFTGIPNFAPELFRLVRLR
DPLKSKALLKGLIELSEEGATQVFRPLNSNQLILGAVGILQFDVVAHRLKYEYKVDCIYESVNIACARWVYSDDDKAMSE
FRTKAYDYLALDGGDMLMYLAPTKVNLTMAEERYPKIKFCATREH

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=488, Percent_Identity=28.8934426229508, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI19923640, Length=510, Percent_Identity=25.4901960784314, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI25306283, Length=394, Percent_Identity=27.6649746192893, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI25306287, Length=294, Percent_Identity=29.5918367346939, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI157426893, Length=168, Percent_Identity=30.952380952381, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI94966754, Length=137, Percent_Identity=29.9270072992701, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1790835, Length=527, Percent_Identity=66.9829222011385, Blast_Score=745, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=495, Percent_Identity=27.2727272727273, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=32.0512820512821, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI48994988, Length=147, Percent_Identity=36.734693877551, Blast_Score=89, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=492, Percent_Identity=28.6585365853659, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17556745, Length=179, Percent_Identity=35.7541899441341, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17557151, Length=146, Percent_Identity=32.8767123287671, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17506493, Length=162, Percent_Identity=30.8641975308642, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=30.8270676691729, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=30.8270676691729, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=480, Percent_Identity=27.7083333333333, Blast_Score=172, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6322359, Length=550, Percent_Identity=24.9090909090909, Blast_Score=120, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6324707, Length=150, Percent_Identity=32.6666666666667, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320593, Length=150, Percent_Identity=32.6666666666667, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323320, Length=138, Percent_Identity=27.536231884058, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24582462, Length=473, Percent_Identity=27.4841437632135, Blast_Score=155, Evalue=7e-38,
Organism=Drosophila melanogaster, GI221458488, Length=518, Percent_Identity=27.027027027027, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI78706572, Length=155, Percent_Identity=31.6129032258064, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24585711, Length=154, Percent_Identity=32.4675324675325, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24585713, Length=154, Percent_Identity=32.4675324675325, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24585709, Length=154, Percent_Identity=32.4675324675325, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59447; Mature: 59316

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDFYQDFNKRRTFAIISHPDAGKTTVTEKLLLFGGAIQLAGTVKGRKADRHATSDWMEM
CCHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHH
EKERGISITTSVMQFVHNQHVINLLDTPGHEDFSEDTYRTLTAVDSALMVIDVAKGVEER
HHHCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHH
TVKLMEVCRLRDTPIMTFINKLDREGREPIDLLDEVESVLGIQCAPITWPVGMGKRFKGI
HHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEECCEEECCCCCHHHHHH
YHRYQDIIYLYQQGSNAKKIDATQIKGLDNPQLDELIGDSAKELREEIELVRGASNEFNL
HHHHHHHEEEEECCCCCCCCCHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCH
QDYLAGKMTPVYFGSAINNFGIKELLDDFVEYAPGPQPRATQERVVSPNEETFSGFVFKI
HHHHHCCCCCEEECCHHCCCCHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCEEEEEE
QANMDPKHRDRIAFVRVCSGSYKKGMKLSHLRIGKEVQISNALTFMAGDRSHTELALAGD
EECCCCCHHCCEEEEEEECCCCCCCCEEEEEECCCEEEECCEEEEEECCCCCCEEEEECC
IIGLHNHGTIRIGDTFTQGEQLKFTGIPNFAPELFRLVRLRDPLKSKALLKGLIELSEEG
EEEECCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
ATQVFRPLNSNQLILGAVGILQFDVVAHRLKYEYKVDCIYESVNIACARWVYSDDDKAMS
HHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCEEEEEEEEECCCEEEEEEEECCCHHHHH
EFRTKAYDYLALDGGDMLMYLAPTKVNLTMAEERYPKIKFCATREH
HHHHHHHEEEEECCCCEEEEEECCEEEEEEHHCCCCCEEEEECCCC
>Mature Secondary Structure 
SDFYQDFNKRRTFAIISHPDAGKTTVTEKLLLFGGAIQLAGTVKGRKADRHATSDWMEM
CHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHH
EKERGISITTSVMQFVHNQHVINLLDTPGHEDFSEDTYRTLTAVDSALMVIDVAKGVEER
HHHCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHH
TVKLMEVCRLRDTPIMTFINKLDREGREPIDLLDEVESVLGIQCAPITWPVGMGKRFKGI
HHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEECCEEECCCCCHHHHHH
YHRYQDIIYLYQQGSNAKKIDATQIKGLDNPQLDELIGDSAKELREEIELVRGASNEFNL
HHHHHHHEEEEECCCCCCCCCHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCH
QDYLAGKMTPVYFGSAINNFGIKELLDDFVEYAPGPQPRATQERVVSPNEETFSGFVFKI
HHHHHCCCCCEEECCHHCCCCHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCEEEEEE
QANMDPKHRDRIAFVRVCSGSYKKGMKLSHLRIGKEVQISNALTFMAGDRSHTELALAGD
EECCCCCHHCCEEEEEEECCCCCCCCEEEEEECCCEEEECCEEEEEECCCCCCEEEEECC
IIGLHNHGTIRIGDTFTQGEQLKFTGIPNFAPELFRLVRLRDPLKSKALLKGLIELSEEG
EEEECCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
ATQVFRPLNSNQLILGAVGILQFDVVAHRLKYEYKVDCIYESVNIACARWVYSDDDKAMS
HHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCEEEEEEEEECCCEEEEEEEECCCHHHHH
EFRTKAYDYLALDGGDMLMYLAPTKVNLTMAEERYPKIKFCATREH
HHHHHHHEEEEECCCCEEEEEECCEEEEEEHHCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA