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The map label for this gene is tilS [H]

Identifier: 54293769

GI number: 54293769

Start: 933637

End: 934932

Strand: Direct

Name: tilS [H]

Synonym: lpl0825

Alternate gene names: 54293769

Gene position: 933637-934932 (Clockwise)

Preceding gene: 54293768

Following gene: 54293770

Centisome position: 27.91

GC content: 40.9

Gene sequence:

>1296_bases
GTGACTAGGCCTTTACTAAGTCCCGAATGGGTAGCTCGCCTTAAACAATTCAAGAAATTGATAGTAGGGTTCAGTGGTGG
TCTGGACTCTACAGTGCTCCTGCATGTCCTTGCCTCAACTCCTCCCTTGTATAATCAACTTTTGGCTGTCCATATTAACC
ATGGCATTAGTGGAAATTCTCTGAACTGGCAAAGGCATTGTGAGCAATTGTGCCTGGATTTAGGTATTGCTTTTATTGCC
AAAGCGATTGAATTTGATCGTTCTGCAAATGTTGAAGAAGCAGCACGCAATGCGAGATATGATTTTTTTTCCTCTTTATT
AGAGGATAATGATTGTCTGGTTTTGGGGCATCATCTTGATGATCAGGCCGAAACAGTTCTTTTGCAACTGTTTCGCGGTG
CAGGAGTTGATGGCTTGGCAGCTATGCAGGAATGCAGCAATTTGGGCGCCGGGCAATTAGCAAGACCATTTTTGACCTGC
CCCAGAGTGGAATTAGAGCATTATGCTCGCATTCATGAATTGAAATGGATAGAAGACGAAAGCAATCAAGATACAAAATA
TTCTCGCAATTATCTGCGCCATCGCGTAATGCCATTATTGCTTGAAAAGTGGCCGGGAGTAGCAGGTAATATTTCCCGAG
CTGCGACTCATTGTCAGCAGGCAAAAGCGAATCTTGAGGTATTGGCTATAAAGGATTGTCCTGATTTGTTAAACGCTACT
GATCATTTGCTGATTGAACCCCTAAAAGGACTTGAATTTGAGCGGATTACAAATGTTTTAAAGTTTTGGCTTAAAAAAAA
TAGAGTTCAATTGCCTTCGTCCAAGACGTTTCAACGTATCATTCATGAAATAATTTTTGCAAAGCTTGATGCCATGCCAA
CAGTGAGTTGGAATCAAATACAAGTACGTCGTTTCCAACAACATCTTTATTTGCTTAAGGCAGACCAGATCAATTTACCG
AAGGCTATTAAATGGCAACAATTTCCAGCTTCTTTAACTTATCCAGACGCCAATATTGAGTTGTCTGCAGTAAAGGCCCA
AAAGGGATTAATGATCCCGAAAGATGCGCAAATAGAAATACGATTCAGAAAGGGTGGCGAAGAGATTTATTGGCATGATC
AAACCAAACATCTAAAAAAATTATTTCAGGAGTGGCAAATTCCCCCCTGGCTAAGGGAGCGAGTTCCATTGGTTTACATT
AATGATCAATTGGCTTGTGTAGTCGGTTATGCAGTGAGCGATTTGTTTTTTACTACTAATCCGCTGGAAGCCTGGTCTAT
AGTTAATAATTCTTAA

Upstream 100 bases:

>100_bases
TTGAAGAGATTATTAACGAGTGAACTCCAGGCTTTGAAAAAGGTGCCTCTGGATCAATTAATTGAGTTAAGGTATCAAAA
GTTTATGGCAATGGGTGCTT

Downstream 100 bases:

>100_bases
TATAACAATCTCCGCTCCCTTACGGTCGCGGCTCGGTTTTAGCCATAACTCAATTTAATAATTTGATTAATCATCCGAGC
CGCGACTGTGAGGGAGCGGA

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MTRPLLSPEWVARLKQFKKLIVGFSGGLDSTVLLHVLASTPPLYNQLLAVHINHGISGNSLNWQRHCEQLCLDLGIAFIA
KAIEFDRSANVEEAARNARYDFFSSLLEDNDCLVLGHHLDDQAETVLLQLFRGAGVDGLAAMQECSNLGAGQLARPFLTC
PRVELEHYARIHELKWIEDESNQDTKYSRNYLRHRVMPLLLEKWPGVAGNISRAATHCQQAKANLEVLAIKDCPDLLNAT
DHLLIEPLKGLEFERITNVLKFWLKKNRVQLPSSKTFQRIIHEIIFAKLDAMPTVSWNQIQVRRFQQHLYLLKADQINLP
KAIKWQQFPASLTYPDANIELSAVKAQKGLMIPKDAQIEIRFRKGGEEIYWHDQTKHLKKLFQEWQIPPWLRERVPLVYI
NDQLACVVGYAVSDLFFTTNPLEAWSIVNNS

Sequences:

>Translated_431_residues
MTRPLLSPEWVARLKQFKKLIVGFSGGLDSTVLLHVLASTPPLYNQLLAVHINHGISGNSLNWQRHCEQLCLDLGIAFIA
KAIEFDRSANVEEAARNARYDFFSSLLEDNDCLVLGHHLDDQAETVLLQLFRGAGVDGLAAMQECSNLGAGQLARPFLTC
PRVELEHYARIHELKWIEDESNQDTKYSRNYLRHRVMPLLLEKWPGVAGNISRAATHCQQAKANLEVLAIKDCPDLLNAT
DHLLIEPLKGLEFERITNVLKFWLKKNRVQLPSSKTFQRIIHEIIFAKLDAMPTVSWNQIQVRRFQQHLYLLKADQINLP
KAIKWQQFPASLTYPDANIELSAVKAQKGLMIPKDAQIEIRFRKGGEEIYWHDQTKHLKKLFQEWQIPPWLRERVPLVYI
NDQLACVVGYAVSDLFFTTNPLEAWSIVNNS
>Mature_430_residues
TRPLLSPEWVARLKQFKKLIVGFSGGLDSTVLLHVLASTPPLYNQLLAVHINHGISGNSLNWQRHCEQLCLDLGIAFIAK
AIEFDRSANVEEAARNARYDFFSSLLEDNDCLVLGHHLDDQAETVLLQLFRGAGVDGLAAMQECSNLGAGQLARPFLTCP
RVELEHYARIHELKWIEDESNQDTKYSRNYLRHRVMPLLLEKWPGVAGNISRAATHCQQAKANLEVLAIKDCPDLLNATD
HLLIEPLKGLEFERITNVLKFWLKKNRVQLPSSKTFQRIIHEIIFAKLDAMPTVSWNQIQVRRFQQHLYLLKADQINLPK
AIKWQQFPASLTYPDANIELSAVKAQKGLMIPKDAQIEIRFRKGGEEIYWHDQTKHLKKLFQEWQIPPWLRERVPLVYIN
DQLACVVGYAVSDLFFTTNPLEAWSIVNNS

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=401, Percent_Identity=35.1620947630923, Blast_Score=243, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 49340; Mature: 49208

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPLLSPEWVARLKQFKKLIVGFSGGLDSTVLLHVLASTPPLYNQLLAVHINHGISGNS
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHEEEECCCCCCC
LNWQRHCEQLCLDLGIAFIAKAIEFDRSANVEEAARNARYDFFSSLLEDNDCLVLGHHLD
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCHHHHHHHHHCCCCEEEEECCCC
DQAETVLLQLFRGAGVDGLAAMQECSNLGAGQLARPFLTCPRVELEHYARIHELKWIEDE
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHEECCC
SNQDTKYSRNYLRHRVMPLLLEKWPGVAGNISRAATHCQQAKANLEVLAIKDCPDLLNAT
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEEEECCCHHHHCCC
DHLLIEPLKGLEFERITNVLKFWLKKNRVQLPSSKTFQRIIHEIIFAKLDAMPTVSWNQI
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHH
QVRRFQQHLYLLKADQINLPKAIKWQQFPASLTYPDANIELSAVKAQKGLMIPKDAQIEI
HHHHHHHHHHEEECCCCCCCCCCCHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEE
RFRKGGEEIYWHDQTKHLKKLFQEWQIPPWLRERVPLVYINDQLACVVGYAVSDLFFTTN
EEECCCCEEEECCHHHHHHHHHHHCCCCHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCC
PLEAWSIVNNS
CHHHHHCCCCC
>Mature Secondary Structure 
TRPLLSPEWVARLKQFKKLIVGFSGGLDSTVLLHVLASTPPLYNQLLAVHINHGISGNS
CCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHEEEECCCCCCC
LNWQRHCEQLCLDLGIAFIAKAIEFDRSANVEEAARNARYDFFSSLLEDNDCLVLGHHLD
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCHHHHHHHHHCCCCEEEEECCCC
DQAETVLLQLFRGAGVDGLAAMQECSNLGAGQLARPFLTCPRVELEHYARIHELKWIEDE
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHEECCC
SNQDTKYSRNYLRHRVMPLLLEKWPGVAGNISRAATHCQQAKANLEVLAIKDCPDLLNAT
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEEEECCCHHHHCCC
DHLLIEPLKGLEFERITNVLKFWLKKNRVQLPSSKTFQRIIHEIIFAKLDAMPTVSWNQI
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHH
QVRRFQQHLYLLKADQINLPKAIKWQQFPASLTYPDANIELSAVKAQKGLMIPKDAQIEI
HHHHHHHHHHEEECCCCCCCCCCCHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEE
RFRKGGEEIYWHDQTKHLKKLFQEWQIPPWLRERVPLVYINDQLACVVGYAVSDLFFTTN
EEECCCCEEEECCHHHHHHHHHHHCCCCHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCC
PLEAWSIVNNS
CHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA