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The map label for this gene is pgk [H]

Identifier: 54293099

GI number: 54293099

Start: 166982

End: 168172

Strand: Reverse

Name: pgk [H]

Synonym: lpl0137

Alternate gene names: 54293099

Gene position: 168172-166982 (Counterclockwise)

Preceding gene: 54293100

Following gene: 54293098

Centisome position: 5.03

GC content: 45.26

Gene sequence:

>1191_bases
ATGAACCTGATTAAAATGAGCGACATTGATCTGTCTGGCAAAAGAGTCCTGATTCGTGAGGATTTAAATGTCCCGATCAA
AGACGGCATGATTACCAGTGATCAAAGATTGCAAGCGGCCCTGCCTACAATCAAATCCGCTTTAGACAGTGGCGCAGCAG
TAATCGTATTGTCTCATCTTGGGCGTCCGGAGGAAGGCAAATACGAAAAAAAATTCTCTTTGGAACCTGTTGCTGATTAT
TTAAGGAAAAACCTGGAATATCCTGTTCGCTTCGTCAAAGATTACCTGAGCGGCGTGGATGTAAACCCTGGGGAATTGGC
CGTATGCGAAAATGTCCGATTTAATCCTGGTGAAAAATCCAATGACGAGTCCCTGGCAAAAAAATTGGCCAACCTTTGCG
ATGTCTTTGTCATGGATGCTTTTGGCACGGCACACAGGGCACAAGCTTCTACCTATGGTGTCGCCCAATATGCTCCAGTT
GCCGTTTCTGGTCCTCTGCTAATCAGGGAACTTGAAGCGTTAAACCAGGTGCTCAAAGCCCCCAAAAAACCTATCGTCGC
CATTGTTGGGGGAGCCAAAGTCTCATCCAAGCTCAGTTTATTGAAACAATTAGTGGGTATGGTCGATGTTCTTATCCCTG
GTGGAGGTATCGCCAATACTTTTCTGAAAGCCCAGGGATTTGAAATTGGTATTTCCCTTTACGAACCTGATTTATTGGAT
GAGGCTCGCCATATCCTTATTCTGGCCAAAGAGAAAGGTTGCCAAATTCCGCTACCCACCGACGTTGTAGTAGGCAAAAC
ATTTAGTGAAACCTGCCCTGCTTTCAACAAATCTCTGTCTAATGTGGCCGCGGACGATATGATCCTTGATATTGGACCAG
AAACCATAAGAGATTATGTAGACCTCATCCATGAAGCCAATACCATCATCTGGAATGGTCCGGTTGGCGTATTCGAATTC
CCGCAGTTTGCTTACGGAACAAGAGCCATTGCTATAGCGATTGCTGAGAGTAATGCCTTTTCTATTGCTGGTGGTGGTGA
TACCTTGGCAGCTGTTGACCTTTATGACTTGAATCAACAAATTTCTTATATTTCCACAGGAGGTGGGGCATTTCTGGAAT
GCCTGGAAGGAAAAACCTTGCCTGCTGTTGCTATTTTGCAGGAGCGCGCAAAACATGTTAAGACGAACTAA

Upstream 100 bases:

>100_bases
AATTTGGTCAAAGTAGTAGCCTGGTATGACAATGAATGGGGTTTCTCTAACCGCATGATTGATACCGCACACCAAATGAT
GAATTGCTAAAGGATAACCA

Downstream 100 bases:

>100_bases
GATTGTTGCCACTTTAGGCCCAGCCAGCAAGGAACCTGAAATTCTGCGATCCATGCTGGCTGCTGGAGTCAACGTAGTAC
GAATTAATTTTTCTCATGCG

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MNLIKMSDIDLSGKRVLIREDLNVPIKDGMITSDQRLQAALPTIKSALDSGAAVIVLSHLGRPEEGKYEKKFSLEPVADY
LRKNLEYPVRFVKDYLSGVDVNPGELAVCENVRFNPGEKSNDESLAKKLANLCDVFVMDAFGTAHRAQASTYGVAQYAPV
AVSGPLLIRELEALNQVLKAPKKPIVAIVGGAKVSSKLSLLKQLVGMVDVLIPGGGIANTFLKAQGFEIGISLYEPDLLD
EARHILILAKEKGCQIPLPTDVVVGKTFSETCPAFNKSLSNVAADDMILDIGPETIRDYVDLIHEANTIIWNGPVGVFEF
PQFAYGTRAIAIAIAESNAFSIAGGGDTLAAVDLYDLNQQISYISTGGGAFLECLEGKTLPAVAILQERAKHVKTN

Sequences:

>Translated_396_residues
MNLIKMSDIDLSGKRVLIREDLNVPIKDGMITSDQRLQAALPTIKSALDSGAAVIVLSHLGRPEEGKYEKKFSLEPVADY
LRKNLEYPVRFVKDYLSGVDVNPGELAVCENVRFNPGEKSNDESLAKKLANLCDVFVMDAFGTAHRAQASTYGVAQYAPV
AVSGPLLIRELEALNQVLKAPKKPIVAIVGGAKVSSKLSLLKQLVGMVDVLIPGGGIANTFLKAQGFEIGISLYEPDLLD
EARHILILAKEKGCQIPLPTDVVVGKTFSETCPAFNKSLSNVAADDMILDIGPETIRDYVDLIHEANTIIWNGPVGVFEF
PQFAYGTRAIAIAIAESNAFSIAGGGDTLAAVDLYDLNQQISYISTGGGAFLECLEGKTLPAVAILQERAKHVKTN
>Mature_396_residues
MNLIKMSDIDLSGKRVLIREDLNVPIKDGMITSDQRLQAALPTIKSALDSGAAVIVLSHLGRPEEGKYEKKFSLEPVADY
LRKNLEYPVRFVKDYLSGVDVNPGELAVCENVRFNPGEKSNDESLAKKLANLCDVFVMDAFGTAHRAQASTYGVAQYAPV
AVSGPLLIRELEALNQVLKAPKKPIVAIVGGAKVSSKLSLLKQLVGMVDVLIPGGGIANTFLKAQGFEIGISLYEPDLLD
EARHILILAKEKGCQIPLPTDVVVGKTFSETCPAFNKSLSNVAADDMILDIGPETIRDYVDLIHEANTIIWNGPVGVFEF
PQFAYGTRAIAIAIAESNAFSIAGGGDTLAAVDLYDLNQQISYISTGGGAFLECLEGKTLPAVAILQERAKHVKTN

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=414, Percent_Identity=39.6135265700483, Blast_Score=272, Evalue=4e-73,
Organism=Homo sapiens, GI31543397, Length=412, Percent_Identity=39.0776699029126, Blast_Score=271, Evalue=1e-72,
Organism=Escherichia coli, GI1789294, Length=391, Percent_Identity=62.4040920716113, Blast_Score=498, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI17508823, Length=413, Percent_Identity=42.8571428571429, Blast_Score=295, Evalue=3e-80,
Organism=Saccharomyces cerevisiae, GI6319857, Length=414, Percent_Identity=40.0966183574879, Blast_Score=258, Evalue=2e-69,
Organism=Drosophila melanogaster, GI17136394, Length=415, Percent_Identity=41.6867469879518, Blast_Score=264, Evalue=7e-71,
Organism=Drosophila melanogaster, GI24581203, Length=413, Percent_Identity=39.9515738498789, Blast_Score=251, Evalue=5e-67,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 42597; Mature: 42597

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLIKMSDIDLSGKRVLIREDLNVPIKDGMITSDQRLQAALPTIKSALDSGAAVIVLSHL
CCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECC
GRPEEGKYEKKFSLEPVADYLRKNLEYPVRFVKDYLSGVDVNPGELAVCENVRFNPGEKS
CCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCC
NDESLAKKLANLCDVFVMDAFGTAHRAQASTYGVAQYAPVAVSGPLLIRELEALNQVLKA
CHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHCCCEEECCHHHHHHHHHHHHHHHC
PKKPIVAIVGGAKVSSKLSLLKQLVGMVDVLIPGGGIANTFLKAQGFEIGISLYEPDLLD
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEEEECCCHHH
EARHILILAKEKGCQIPLPTDVVVGKTFSETCPAFNKSLSNVAADDMILDIGPETIRDYV
CCCEEEEEECCCCCCCCCCCCEEECCCHHHHCCHHHHHHHHHHHCCEEEECCHHHHHHHH
DLIHEANTIIWNGPVGVFEFPQFAYGTRAIAIAIAESNAFSIAGGGDTLAAVDLYDLNQQ
HHHHHCCEEEECCCCCCCCCCCHHCCCEEEEEEEECCCEEEEECCCCCEEEEEEECCCCC
ISYISTGGGAFLECLEGKTLPAVAILQERAKHVKTN
EEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNLIKMSDIDLSGKRVLIREDLNVPIKDGMITSDQRLQAALPTIKSALDSGAAVIVLSHL
CCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECC
GRPEEGKYEKKFSLEPVADYLRKNLEYPVRFVKDYLSGVDVNPGELAVCENVRFNPGEKS
CCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCC
NDESLAKKLANLCDVFVMDAFGTAHRAQASTYGVAQYAPVAVSGPLLIRELEALNQVLKA
CHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHCCCEEECCHHHHHHHHHHHHHHHC
PKKPIVAIVGGAKVSSKLSLLKQLVGMVDVLIPGGGIANTFLKAQGFEIGISLYEPDLLD
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEEEECCCHHH
EARHILILAKEKGCQIPLPTDVVVGKTFSETCPAFNKSLSNVAADDMILDIGPETIRDYV
CCCEEEEEECCCCCCCCCCCCEEECCCHHHHCCHHHHHHHHHHHCCEEEECCHHHHHHHH
DLIHEANTIIWNGPVGVFEFPQFAYGTRAIAIAIAESNAFSIAGGGDTLAAVDLYDLNQQ
HHHHHCCEEEECCCCCCCCCCCHHCCCEEEEEEEECCCEEEEECCCCCEEEEEEECCCCC
ISYISTGGGAFLECLEGKTLPAVAILQERAKHVKTN
EEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA