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The map label for this gene is pykA [H]

Identifier: 54293098

GI number: 54293098

Start: 165574

End: 166998

Strand: Reverse

Name: pykA [H]

Synonym: lpl0136

Alternate gene names: 54293098

Gene position: 166998-165574 (Counterclockwise)

Preceding gene: 54293099

Following gene: 54293097

Centisome position: 4.99

GC content: 42.6

Gene sequence:

>1425_bases
ATGTTAAGACGAACTAAGATTGTTGCCACTTTAGGCCCAGCCAGCAAGGAACCTGAAATTCTGCGATCCATGCTGGCTGC
TGGAGTCAACGTAGTACGAATTAATTTTTCTCATGCGGATTCCTCCGCCCTGCAATTAATTGCACTGGTTCGTAAAATCG
CAGATGAATTAAACCACCCGGTTGCAGTCATGGCTGACTTACAAGGGCCTAAAATAAGAGTCGGCCGTTTTCAAAACAAA
TCCATCACTTTAATTGATGGACAAAATTTTACATTGGATTGTATGGCCCCAGATACTCTGGGAGACATAAGTGGCGTATC
GGTCGCTTATCCTAATTTGGCCAATGAACTCAGTATTGGTGATCATCTTCTGATTAATGATGGGTTAATTGAATTGGAAG
TTATAGAGACTTCCGGGTCAAAAATTCATTGCAAGGTAGTGGAAGGTGGAGTACTCACTGATTTGAAAGGTTTGAATCGA
AAAGGTGGCGGGTTAGCAGCCAGAACTTTAACAGAAAAAGATAGAAATGATTTACGTACAGCAATTGAAGCAGAAGTGGA
CTACATTAGTTTATCTTTTGTAAAAGACGCAGAGGATATCCGGCAAGCAAGAGCGCTAATGAAAGACTATGGAGCCCAAA
TAACCCCTATTATCGCTAAAATTGAGCGCATGGAAGCTTTGGATCACTTAACTGATATTATTCGCGAAGCAGACGCCATC
ATGGTGGCCAGAGGAGATTTAGGCGTTGAAGTGGGTGCTGCTGAAGTTCCCGCTATTCAAAAACACATCATAGAACAAAC
GCGACTTTTAGATAAAGTGGTGATTACCGCAACACAAATGATGGAATCCATGATAAGTAACCCTCAGCCTACCCGTGCCG
AGGTTTCTGATGTAGCCAACGCGATTCTGGACGGAACTGATGCAGTGATGCTTTCGGCTGAAACAGCAAGCGGCCTTTTT
CCAGTCAAAGTCATTACCATGGTCAATAAAATATGTTTAAGCGCTGAGAAGCATGCCAGCTTTTTTTATCATAGCGACCC
GGAAACCTGCCATTATCAACGTGCCGATCAGGCAATCGCTATGGCTACCATGCATACTGCCAATCATTTTCCCATTCAGG
CGATTATCACACTGACCGAATCCGGCGATACGGCTTTATGGATATCCCGACAACACAGCACTGTACCTATTTTTGCCATA
TCAGCCAACAAGAGAACCATTGGCCGATTAAGCCTCGTAAATAATGTCTTTCCTATTTACATTGATTTTCATCAATTCAA
TCCTGAAGGATTGAATCAACAAATTTTGCATGAATTAGTCAAATCAGGGCACCTTGAAAAGAAAGGCTATGTGTTATTAA
CACGTGGAACCCAAATTGGAACTCCAGGAGGCACTAACTGCATGGAAATTATACCTGTCATTTGA

Upstream 100 bases:

>100_bases
CAACAAATTTCTTATATTTCCACAGGAGGTGGGGCATTTCTGGAATGCCTGGAAGGAAAAACCTTGCCTGCTGTTGCTAT
TTTGCAGGAGCGCGCAAAAC

Downstream 100 bases:

>100_bases
GCGCCTGGCATCTTGTTAGTTAAAGCAGTGTTCTTTCATAAACGATAAAAATGATTATTTTAATGGCACTTTAATCAAAC
TATCACCTCTATGCTACTAT

Product: hypothetical protein

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MLRRTKIVATLGPASKEPEILRSMLAAGVNVVRINFSHADSSALQLIALVRKIADELNHPVAVMADLQGPKIRVGRFQNK
SITLIDGQNFTLDCMAPDTLGDISGVSVAYPNLANELSIGDHLLINDGLIELEVIETSGSKIHCKVVEGGVLTDLKGLNR
KGGGLAARTLTEKDRNDLRTAIEAEVDYISLSFVKDAEDIRQARALMKDYGAQITPIIAKIERMEALDHLTDIIREADAI
MVARGDLGVEVGAAEVPAIQKHIIEQTRLLDKVVITATQMMESMISNPQPTRAEVSDVANAILDGTDAVMLSAETASGLF
PVKVITMVNKICLSAEKHASFFYHSDPETCHYQRADQAIAMATMHTANHFPIQAIITLTESGDTALWISRQHSTVPIFAI
SANKRTIGRLSLVNNVFPIYIDFHQFNPEGLNQQILHELVKSGHLEKKGYVLLTRGTQIGTPGGTNCMEIIPVI

Sequences:

>Translated_474_residues
MLRRTKIVATLGPASKEPEILRSMLAAGVNVVRINFSHADSSALQLIALVRKIADELNHPVAVMADLQGPKIRVGRFQNK
SITLIDGQNFTLDCMAPDTLGDISGVSVAYPNLANELSIGDHLLINDGLIELEVIETSGSKIHCKVVEGGVLTDLKGLNR
KGGGLAARTLTEKDRNDLRTAIEAEVDYISLSFVKDAEDIRQARALMKDYGAQITPIIAKIERMEALDHLTDIIREADAI
MVARGDLGVEVGAAEVPAIQKHIIEQTRLLDKVVITATQMMESMISNPQPTRAEVSDVANAILDGTDAVMLSAETASGLF
PVKVITMVNKICLSAEKHASFFYHSDPETCHYQRADQAIAMATMHTANHFPIQAIITLTESGDTALWISRQHSTVPIFAI
SANKRTIGRLSLVNNVFPIYIDFHQFNPEGLNQQILHELVKSGHLEKKGYVLLTRGTQIGTPGGTNCMEIIPVI
>Mature_474_residues
MLRRTKIVATLGPASKEPEILRSMLAAGVNVVRINFSHADSSALQLIALVRKIADELNHPVAVMADLQGPKIRVGRFQNK
SITLIDGQNFTLDCMAPDTLGDISGVSVAYPNLANELSIGDHLLINDGLIELEVIETSGSKIHCKVVEGGVLTDLKGLNR
KGGGLAARTLTEKDRNDLRTAIEAEVDYISLSFVKDAEDIRQARALMKDYGAQITPIIAKIERMEALDHLTDIIREADAI
MVARGDLGVEVGAAEVPAIQKHIIEQTRLLDKVVITATQMMESMISNPQPTRAEVSDVANAILDGTDAVMLSAETASGLF
PVKVITMVNKICLSAEKHASFFYHSDPETCHYQRADQAIAMATMHTANHFPIQAIITLTESGDTALWISRQHSTVPIFAI
SANKRTIGRLSLVNNVFPIYIDFHQFNPEGLNQQILHELVKSGHLEKKGYVLLTRGTQIGTPGGTNCMEIIPVI

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=491, Percent_Identity=33.8085539714868, Blast_Score=256, Evalue=4e-68,
Organism=Homo sapiens, GI33286420, Length=491, Percent_Identity=33.8085539714868, Blast_Score=256, Evalue=4e-68,
Organism=Homo sapiens, GI32967597, Length=494, Percent_Identity=35.4251012145749, Blast_Score=255, Evalue=6e-68,
Organism=Homo sapiens, GI10835121, Length=494, Percent_Identity=35.4251012145749, Blast_Score=255, Evalue=6e-68,
Organism=Homo sapiens, GI33286418, Length=499, Percent_Identity=33.4669338677355, Blast_Score=248, Evalue=1e-65,
Organism=Homo sapiens, GI310128732, Length=289, Percent_Identity=34.6020761245675, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI310128730, Length=289, Percent_Identity=34.6020761245675, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=31.6455696202532, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=31.6455696202532, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI310128738, Length=216, Percent_Identity=30.0925925925926, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1788160, Length=477, Percent_Identity=45.4926624737945, Blast_Score=384, Evalue=1e-108,
Organism=Escherichia coli, GI1787965, Length=439, Percent_Identity=37.129840546697, Blast_Score=288, Evalue=5e-79,
Organism=Caenorhabditis elegans, GI17544584, Length=427, Percent_Identity=37.7049180327869, Blast_Score=262, Evalue=3e-70,
Organism=Caenorhabditis elegans, GI71984413, Length=487, Percent_Identity=36.1396303901437, Blast_Score=238, Evalue=6e-63,
Organism=Caenorhabditis elegans, GI17506829, Length=487, Percent_Identity=36.1396303901437, Blast_Score=238, Evalue=7e-63,
Organism=Caenorhabditis elegans, GI17506831, Length=487, Percent_Identity=36.5503080082135, Blast_Score=238, Evalue=7e-63,
Organism=Caenorhabditis elegans, GI71984406, Length=487, Percent_Identity=36.1396303901437, Blast_Score=237, Evalue=8e-63,
Organism=Saccharomyces cerevisiae, GI6319279, Length=430, Percent_Identity=34.8837209302326, Blast_Score=240, Evalue=4e-64,
Organism=Saccharomyces cerevisiae, GI6324923, Length=485, Percent_Identity=31.9587628865979, Blast_Score=223, Evalue=5e-59,
Organism=Drosophila melanogaster, GI24648964, Length=498, Percent_Identity=33.9357429718875, Blast_Score=241, Evalue=8e-64,
Organism=Drosophila melanogaster, GI28571814, Length=498, Percent_Identity=33.9357429718875, Blast_Score=241, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24648966, Length=450, Percent_Identity=35.5555555555556, Blast_Score=228, Evalue=9e-60,
Organism=Drosophila melanogaster, GI24581235, Length=429, Percent_Identity=33.3333333333333, Blast_Score=212, Evalue=5e-55,
Organism=Drosophila melanogaster, GI24646914, Length=281, Percent_Identity=35.2313167259786, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51616; Mature: 51616

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRRTKIVATLGPASKEPEILRSMLAAGVNVVRINFSHADSSALQLIALVRKIADELNHP
CCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCC
VAVMADLQGPKIRVGRFQNKSITLIDGQNFTLDCMAPDTLGDISGVSVAYPNLANELSIG
EEEEEECCCCEEEEEEECCCEEEEECCCCEEEEEECCCCCCCCCCCEEECCCCCCCCCCC
DHLLINDGLIELEVIETSGSKIHCKVVEGGVLTDLKGLNRKGGGLAARTLTEKDRNDLRT
CEEEEECCEEEEEEEECCCCEEEEEEECCCCEECHHCCCCCCCCEEEEHHHHHHHHHHHH
AIEAEVDYISLSFVKDAEDIRQARALMKDYGAQITPIIAKIERMEALDHLTDIIREADAI
HHHHHCCEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEE
MVARGDLGVEVGAAEVPAIQKHIIEQTRLLDKVVITATQMMESMISNPQPTRAEVSDVAN
EEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
AILDGTDAVMLSAETASGLFPVKVITMVNKICLSAEKHASFFYHSDPETCHYQRADQAIA
HHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHCCEEECCCCCCCHHHHHHHHHH
MATMHTANHFPIQAIITLTESGDTALWISRQHSTVPIFAISANKRTIGRLSLVNNVFPIY
HHHHHCCCCCCEEEEEEEECCCCEEEEEECCCCEEEEEEEECCCCCHHHHHHHCCCEEEE
IDFHQFNPEGLNQQILHELVKSGHLEKKGYVLLTRGTQIGTPGGTNCMEIIPVI
EEEEECCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCEECCCCCCCHHEEEECC
>Mature Secondary Structure
MLRRTKIVATLGPASKEPEILRSMLAAGVNVVRINFSHADSSALQLIALVRKIADELNHP
CCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCC
VAVMADLQGPKIRVGRFQNKSITLIDGQNFTLDCMAPDTLGDISGVSVAYPNLANELSIG
EEEEEECCCCEEEEEEECCCEEEEECCCCEEEEEECCCCCCCCCCCEEECCCCCCCCCCC
DHLLINDGLIELEVIETSGSKIHCKVVEGGVLTDLKGLNRKGGGLAARTLTEKDRNDLRT
CEEEEECCEEEEEEEECCCCEEEEEEECCCCEECHHCCCCCCCCEEEEHHHHHHHHHHHH
AIEAEVDYISLSFVKDAEDIRQARALMKDYGAQITPIIAKIERMEALDHLTDIIREADAI
HHHHHCCEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEE
MVARGDLGVEVGAAEVPAIQKHIIEQTRLLDKVVITATQMMESMISNPQPTRAEVSDVAN
EEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
AILDGTDAVMLSAETASGLFPVKVITMVNKICLSAEKHASFFYHSDPETCHYQRADQAIA
HHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHCCEEECCCCCCCHHHHHHHHHH
MATMHTANHFPIQAIITLTESGDTALWISRQHSTVPIFAISANKRTIGRLSLVNNVFPIY
HHHHHCCCCCCEEEEEEEECCCCEEEEEECCCCEEEEEEEECCCCCHHHHHHHCCCEEEE
IDFHQFNPEGLNQQILHELVKSGHLEKKGYVLLTRGTQIGTPGGTNCMEIIPVI
EEEEECCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCEECCCCCCCHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]