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The map label for this gene is pit [H]

Identifier: 54027225

GI number: 54027225

Start: 5548103

End: 5549272

Strand: Reverse

Name: pit [H]

Synonym: nfa52510

Alternate gene names: 54027225

Gene position: 5549272-5548103 (Counterclockwise)

Preceding gene: 54027226

Following gene: 54027224

Centisome position: 92.16

GC content: 71.11

Gene sequence:

>1170_bases
GTGACCGCCGAATTACTGGTGCTGCTGATCGTCATCGCGACGGCACTCACATTCGATTTCACCAACGGCTTCCACGACAC
CGCCAACGCAATGGCCACCTCCATCGCCACCGGGGCGCTGCGGCCACGCATCGCGGTGGCCCTCTCGGCGGCGCTGAACC
TCATCGGCGCCTTCCTCTCGGTCGCGGTGGCCGCGACCGTCGCCAAGGGCATCGTCCAGCTCGACGCGGTGGGCGGGCAG
GACCTGCTGGTGATCGTCTTCGCCGGGTTGGTCGGCGCGATTCTCTGGAACGTCTTCACCTGGTTGCTCGGCCTGCCGTC
GAGCTCGTCGCACGCGCTGTTCGGCGGTCTCATCGGCGCCACCGTCGCCGCGCTCGGCTGGGGCGGGGTGATCTGGGCGT
CGGGCTCCGAGGGCGTGCTGACCAAGATCGTGCTGCCCGCGGTGCTGGCGCCGGTGGTGGCCGCGCTGGTCTCGACCATC
GGCACCTGGGGCGTGTACCGGGTCACCGCGTCCTCGGATCAGGCCAAGGTGCGCTCGGGCTTCCGCTGGGGGCAGGTGGG
CTCGGCGTCGCTGGTCTCGCTGGCGCACGGCACCAACGACGCGCAGAAGACGATGGGCGTGATCTTCCTTGCGCTGGTCG
CGCACGGCACGCTCACCAAGGACGACGAGATGCCGCTGTGGGTGATGGCCGCCTGCGCGTTCGCCATCGCCGCGGGCACC
TACCTGGGCGGCTGGCGGATCATCCGCACCCTGGGCAAGGGCCTGGTGGAGATCGACTCGCCGCAGGGCCTGGCCGCGGA
GTCCTCCTCGGCCGCGATCATCCTGACCTCCGCGCATTTCGGGCTGCCGCTCTCGACGACGCAGACCGCCACCGGCTCGA
TCCTGGGCAGCGGCATCGGCAAGGGCGCCGAGGTGCGCTGGGCGGTGATGGGCCGCATGGCGGTGGCCTGGCTGCTGACG
CTGCCGATGGCCGGCCTGGCGGGCGCGATCTGCTGGGCCGTCGCCCACCTGATCGGCGGGTTCGCGGGCGTGCTCGTGGT
GTTCGCCGTGCTGGTGGTGCTGTGCACGCTGATGTGGGTGCGCTCGCGGCGCGACAAGGTCGACACCACCAACGTCAACG
AGTGGCCGGGCACCGGCGCGCACCCGACCCCCCGATCCCCCTCGCTGTAG

Upstream 100 bases:

>100_bases
CCCTGATCCGGTGAGGCGCGCACTTTAGCGCATCGGGGTGAACGCCCGACACGCGGCGACCGCCTGGCAGGCGAACAGAC
GGTTACCGCACACTGGAGAC

Downstream 100 bases:

>100_bases
AAGGAGTCCGCAGTGCACACCCTCGCCACGAACCTGTCCGCGCTGTGGCAGGTCGTCGTCGCCGCGCTGGTGCTCGGCGC
GGGCCTGCCGATCGTCTTCG

Product: putative phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MTAELLVLLIVIATALTFDFTNGFHDTANAMATSIATGALRPRIAVALSAALNLIGAFLSVAVAATVAKGIVQLDAVGGQ
DLLVIVFAGLVGAILWNVFTWLLGLPSSSSHALFGGLIGATVAALGWGGVIWASGSEGVLTKIVLPAVLAPVVAALVSTI
GTWGVYRVTASSDQAKVRSGFRWGQVGSASLVSLAHGTNDAQKTMGVIFLALVAHGTLTKDDEMPLWVMAACAFAIAAGT
YLGGWRIIRTLGKGLVEIDSPQGLAAESSSAAIILTSAHFGLPLSTTQTATGSILGSGIGKGAEVRWAVMGRMAVAWLLT
LPMAGLAGAICWAVAHLIGGFAGVLVVFAVLVVLCTLMWVRSRRDKVDTTNVNEWPGTGAHPTPRSPSL

Sequences:

>Translated_389_residues
MTAELLVLLIVIATALTFDFTNGFHDTANAMATSIATGALRPRIAVALSAALNLIGAFLSVAVAATVAKGIVQLDAVGGQ
DLLVIVFAGLVGAILWNVFTWLLGLPSSSSHALFGGLIGATVAALGWGGVIWASGSEGVLTKIVLPAVLAPVVAALVSTI
GTWGVYRVTASSDQAKVRSGFRWGQVGSASLVSLAHGTNDAQKTMGVIFLALVAHGTLTKDDEMPLWVMAACAFAIAAGT
YLGGWRIIRTLGKGLVEIDSPQGLAAESSSAAIILTSAHFGLPLSTTQTATGSILGSGIGKGAEVRWAVMGRMAVAWLLT
LPMAGLAGAICWAVAHLIGGFAGVLVVFAVLVVLCTLMWVRSRRDKVDTTNVNEWPGTGAHPTPRSPSL
>Mature_388_residues
TAELLVLLIVIATALTFDFTNGFHDTANAMATSIATGALRPRIAVALSAALNLIGAFLSVAVAATVAKGIVQLDAVGGQD
LLVIVFAGLVGAILWNVFTWLLGLPSSSSHALFGGLIGATVAALGWGGVIWASGSEGVLTKIVLPAVLAPVVAALVSTIG
TWGVYRVTASSDQAKVRSGFRWGQVGSASLVSLAHGTNDAQKTMGVIFLALVAHGTLTKDDEMPLWVMAACAFAIAAGTY
LGGWRIIRTLGKGLVEIDSPQGLAAESSSAAIILTSAHFGLPLSTTQTATGSILGSGIGKGAEVRWAVMGRMAVAWLLTL
PMAGLAGAICWAVAHLIGGFAGVLVVFAVLVVLCTLMWVRSRRDKVDTTNVNEWPGTGAHPTPRSPSL

Specific function: Low-affinity inorganic phosphate transport [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI5803173, Length=152, Percent_Identity=33.5526315789474, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI31543630, Length=171, Percent_Identity=29.2397660818713, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI1789907, Length=224, Percent_Identity=33.0357142857143, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789360, Length=217, Percent_Identity=33.1797235023041, Blast_Score=93, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71981576, Length=170, Percent_Identity=30, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17539280, Length=176, Percent_Identity=30.6818181818182, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI32566716, Length=184, Percent_Identity=29.3478260869565, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25146401, Length=161, Percent_Identity=27.3291925465839, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17536725, Length=178, Percent_Identity=27.5280898876405, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319773, Length=171, Percent_Identity=29.8245614035088, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21356511, Length=212, Percent_Identity=25.4716981132075, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 39844; Mature: 39713

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAELLVLLIVIATALTFDFTNGFHDTANAMATSIATGALRPRIAVALSAALNLIGAFLS
CCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VAVAATVAKGIVQLDAVGGQDLLVIVFAGLVGAILWNVFTWLLGLPSSSSHALFGGLIGA
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
TVAALGWGGVIWASGSEGVLTKIVLPAVLAPVVAALVSTIGTWGVYRVTASSDQAKVRSG
HHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCC
FRWGQVGSASLVSLAHGTNDAQKTMGVIFLALVAHGTLTKDDEMPLWVMAACAFAIAAGT
CCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
YLGGWRIIRTLGKGLVEIDSPQGLAAESSSAAIILTSAHFGLPLSTTQTATGSILGSGIG
HHHHHHHHHHHCCCCEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHCCC
KGAEVRWAVMGRMAVAWLLTLPMAGLAGAICWAVAHLIGGFAGVLVVFAVLVVLCTLMWV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSRRDKVDTTNVNEWPGTGAHPTPRSPSL
HHHCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TAELLVLLIVIATALTFDFTNGFHDTANAMATSIATGALRPRIAVALSAALNLIGAFLS
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VAVAATVAKGIVQLDAVGGQDLLVIVFAGLVGAILWNVFTWLLGLPSSSSHALFGGLIGA
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
TVAALGWGGVIWASGSEGVLTKIVLPAVLAPVVAALVSTIGTWGVYRVTASSDQAKVRSG
HHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCC
FRWGQVGSASLVSLAHGTNDAQKTMGVIFLALVAHGTLTKDDEMPLWVMAACAFAIAAGT
CCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
YLGGWRIIRTLGKGLVEIDSPQGLAAESSSAAIILTSAHFGLPLSTTQTATGSILGSGIG
HHHHHHHHHHHCCCCEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHCCC
KGAEVRWAVMGRMAVAWLLTLPMAGLAGAICWAVAHLIGGFAGVLVVFAVLVVLCTLMWV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSRRDKVDTTNVNEWPGTGAHPTPRSPSL
HHHCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]