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The map label for this gene is ligB

Identifier: 54027226

GI number: 54027226

Start: 5549367

End: 5550938

Strand: Reverse

Name: ligB

Synonym: nfa52520

Alternate gene names: 54027226

Gene position: 5550938-5549367 (Counterclockwise)

Preceding gene: 54027234

Following gene: 54027225

Centisome position: 92.19

GC content: 74.43

Gene sequence:

>1572_bases
GTGCTGTTCGCTGACGTGGTGCGGACCTCGGAGGCGGTGCGGGCCACCAGGTCGAGGAAGACGAAGGTCGCGGCGCTGGC
GGAGCTGCTGCGCGCGGCGGAGTCCGCGGAGCTGGCCCCGGTGGTCGCGTGGCTGTCCGGTGAGTTGCGGCAGGGCCGCA
TCGGCACCGGCTGGCGCACGCTCACCGGGGTGCGCGTGCCACCGGCGCTGGACGCCGCGCTGGAGGTCGCGACGGTCGAC
GCGATCTTCGACGAACTGGCCGCCGTGTCGGGCGCCGGCTCGGGGAATCGTCGCAAGGAACTGCTGACCCGGCTGTGGTC
GGCGGCGACCGAGCCCGAACAGGAGTTCCTGCTGCGGCTGCTCACCGGGGAACTGCGGCAGGGCGCGCTCACCGCGTTGG
TCGCCGAGGCGGTCGCCGCGGCCGCGGACGTGCCGGTCGAGCAGGTGCGCCGGGCCTACATGCTCTCCGGGCAGCTGCCC
GTGACGGCGGAGGCCGCCCTGCGCGGTGGCGCGGCGGCGCTGGCGGAGTTCCGGCTCGAGGTGGGCAGGCCGATCCAGCC
GATGCTCGCCGCGCCGGGCGCCTCGCTCGAGGAGGCGATGACGGAGTTCGGCGGGGAGGTCAGCGTCGAGCACAAGCTCG
ACGGCGCGCGCATCCAGGTCCACCGCGACGGCGACCGGATCGCCGTGTTCACCCGCACGCTGCGCGACATCACCGCGGGC
GTCCCGGAACTGGTGGAGCTGGTGGCCCGGCTCGACTGCACCAGCGTGGTGCTCGACGGGGAGACGCTCGCGCTGACCGA
CGCGGGCCGCCCGCGCCCGTTCCAGGAGACGATGAGCCGCTTCGCCACCGCCGACCCCGCCCGCGCGGCCGAGGTCGACC
TGCCGCCGGGCACGCCGGTGGTGCCGCCGGTGTCCTCGACGCGCGAACTGCTGCTGCACCCGTACTTCTTCGACTGCCTG
CACCTGGACGGCCGCGACCTGCTCGACGCCCCGTTGTCCGAGCGCCGGGCCGCGCTGCTGGCGGTCGTGGGCGAACACGC
CATCCCGGCGCTCATCCGGCCCGAGCCCGAGGCCGCCGCCGAGTACTTCGACGGCGCGCTGGCGGCCGGACACGAGGGGC
TGATGGTCAAGTCGCTGAGCGCGCCGTACGCGGCGGGCAGACGCGGGCGGTCCTGGCAGAAGATCAAGCCGACGCACACC
CTCGACCTGCTCGTGCTCGGTGCGGAGTGGGGGTACGGGCGGCGCACCGGGTACCTGTCCAACCTGCACCTGGGCGCGCG
CGATCCGCGCACCGGCGAGCCGGTGATGGTGGGCAAGACCTTCAAGGGCCTCACCGACGCCCTGCTGGCCTGGCAGACCG
CGGAGTTCCCGCGCCACGAGCGGGCCCGTGACGAGCACACCGTGTACCTGTGGCCGGAGCTGGTGGTGGAGATCGCGCTG
GACGGTGTGCAGGTGAGCCCGCGGTACCCCGGCGGCGTCGCGCTGCGGTTCGCGCGGGTGGTGCGCTATCGGCCCGACAA
GACGCCCGACCAGGCCGACACCATCGATACCGTGCGCGGCCTGCTGCCCTGA

Upstream 100 bases:

>100_bases
GAAATTAACAGGATGTGCGAGAGCGCGGGGAGCGTCCGCCGTCGCGCGGTCCTGGCGTGGATCCGTGCGGATGTCGGTAC
CGGCGGTTACGGTCTGATTC

Downstream 100 bases:

>100_bases
TCCGGTGAGGCGCGCACTTTAGCGCATCGGGGTGAACGCCCGACACGCGGCGACCGCCTGGCAGGCGAACAGACGGTTAC
CGCACACTGGAGACGTGACC

Product: ATP-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP]

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MLFADVVRTSEAVRATRSRKTKVAALAELLRAAESAELAPVVAWLSGELRQGRIGTGWRTLTGVRVPPALDAALEVATVD
AIFDELAAVSGAGSGNRRKELLTRLWSAATEPEQEFLLRLLTGELRQGALTALVAEAVAAAADVPVEQVRRAYMLSGQLP
VTAEAALRGGAAALAEFRLEVGRPIQPMLAAPGASLEEAMTEFGGEVSVEHKLDGARIQVHRDGDRIAVFTRTLRDITAG
VPELVELVARLDCTSVVLDGETLALTDAGRPRPFQETMSRFATADPARAAEVDLPPGTPVVPPVSSTRELLLHPYFFDCL
HLDGRDLLDAPLSERRAALLAVVGEHAIPALIRPEPEAAAEYFDGALAAGHEGLMVKSLSAPYAAGRRGRSWQKIKPTHT
LDLLVLGAEWGYGRRTGYLSNLHLGARDPRTGEPVMVGKTFKGLTDALLAWQTAEFPRHERARDEHTVYLWPELVVEIAL
DGVQVSPRYPGGVALRFARVVRYRPDKTPDQADTIDTVRGLLP

Sequences:

>Translated_523_residues
MLFADVVRTSEAVRATRSRKTKVAALAELLRAAESAELAPVVAWLSGELRQGRIGTGWRTLTGVRVPPALDAALEVATVD
AIFDELAAVSGAGSGNRRKELLTRLWSAATEPEQEFLLRLLTGELRQGALTALVAEAVAAAADVPVEQVRRAYMLSGQLP
VTAEAALRGGAAALAEFRLEVGRPIQPMLAAPGASLEEAMTEFGGEVSVEHKLDGARIQVHRDGDRIAVFTRTLRDITAG
VPELVELVARLDCTSVVLDGETLALTDAGRPRPFQETMSRFATADPARAAEVDLPPGTPVVPPVSSTRELLLHPYFFDCL
HLDGRDLLDAPLSERRAALLAVVGEHAIPALIRPEPEAAAEYFDGALAAGHEGLMVKSLSAPYAAGRRGRSWQKIKPTHT
LDLLVLGAEWGYGRRTGYLSNLHLGARDPRTGEPVMVGKTFKGLTDALLAWQTAEFPRHERARDEHTVYLWPELVVEIAL
DGVQVSPRYPGGVALRFARVVRYRPDKTPDQADTIDTVRGLLP
>Mature_523_residues
MLFADVVRTSEAVRATRSRKTKVAALAELLRAAESAELAPVVAWLSGELRQGRIGTGWRTLTGVRVPPALDAALEVATVD
AIFDELAAVSGAGSGNRRKELLTRLWSAATEPEQEFLLRLLTGELRQGALTALVAEAVAAAADVPVEQVRRAYMLSGQLP
VTAEAALRGGAAALAEFRLEVGRPIQPMLAAPGASLEEAMTEFGGEVSVEHKLDGARIQVHRDGDRIAVFTRTLRDITAG
VPELVELVARLDCTSVVLDGETLALTDAGRPRPFQETMSRFATADPARAAEVDLPPGTPVVPPVSSTRELLLHPYFFDCL
HLDGRDLLDAPLSERRAALLAVVGEHAIPALIRPEPEAAAEYFDGALAAGHEGLMVKSLSAPYAAGRRGRSWQKIKPTHT
LDLLVLGAEWGYGRRTGYLSNLHLGARDPRTGEPVMVGKTFKGLTDALLAWQTAEFPRHERARDEHTVYLWPELVVEIAL
DGVQVSPRYPGGVALRFARVVRYRPDKTPDQADTIDTVRGLLP

Specific function: DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family

Homologues:

Organism=Homo sapiens, GI4557719, Length=531, Percent_Identity=27.1186440677966, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI73747829, Length=532, Percent_Identity=25, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI73747844, Length=532, Percent_Identity=25, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI46255052, Length=499, Percent_Identity=24.8496993987976, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI23199993, Length=499, Percent_Identity=24.8496993987976, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI148539894, Length=499, Percent_Identity=24.8496993987976, Blast_Score=110, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71985269, Length=525, Percent_Identity=28.952380952381, Blast_Score=176, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6320038, Length=529, Percent_Identity=27.2211720226843, Blast_Score=149, Evalue=9e-37,
Organism=Drosophila melanogaster, GI24762424, Length=487, Percent_Identity=27.5154004106776, Blast_Score=139, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLI_NOCFA (Q5YNY7)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_121468.1
- HSSP:   P18858
- ProteinModelPortal:   Q5YNY7
- SMR:   Q5YNY7
- GeneID:   3110671
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa52520
- NMPDR:   fig|247156.1.peg.5047
- HOGENOM:   HBG570821
- OMA:   GRPRPFQ
- ProtClustDB:   PRK03180
- BioCyc:   NFAR247156:NFA52520-MONOMER
- HAMAP:   MF_00407
- InterPro:   IPR022865
- InterPro:   IPR000977
- InterPro:   IPR012309
- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012308
- InterPro:   IPR012340
- InterPro:   IPR016027
- Gene3D:   G3DSA:2.40.50.140
- TIGRFAMs:   TIGR00574

Pfam domain/function: PF04679 DNA_ligase_A_C; PF01068 DNA_ligase_A_M; PF04675 DNA_ligase_A_N; SSF50249 Nucleic_acid_OB

EC number: =6.5.1.1

Molecular weight: Translated: 56457; Mature: 56457

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00697 DNA_LIGASE_A1; PS00333 DNA_LIGASE_A2; PS50160 DNA_LIGASE_A3

Important sites: ACT_SITE 212-212 BINDING 210-210 BINDING 217-217 BINDING 232-232 BINDING 261-261 BINDING 317-317 BINDING 388-388 BINDING 394-394

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFADVVRTSEAVRATRSRKTKVAALAELLRAAESAELAPVVAWLSGELRQGRIGTGWRT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCEE
LTGVRVPPALDAALEVATVDAIFDELAAVSGAGSGNRRKELLTRLWSAATEPEQEFLLRL
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
LTGELRQGALTALVAEAVAAAADVPVEQVRRAYMLSGQLPVTAEAALRGGAAALAEFRLE
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHH
VGRPIQPMLAAPGASLEEAMTEFGGEVSVEHKLDGARIQVHRDGDRIAVFTRTLRDITAG
CCCCCCHHHCCCCCCHHHHHHHCCCCEEEEECCCCCEEEEEECCCEEEHHHHHHHHHHCC
VPELVELVARLDCTSVVLDGETLALTDAGRPRPFQETMSRFATADPARAAEVDLPPGTPV
HHHHHHHHHHCCCCEEEECCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCC
VPPVSSTRELLLHPYFFDCLHLDGRDLLDAPLSERRAALLAVVGEHAIPALIRPEPEAAA
CCCCCCCHHHHHCHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
EYFDGALAAGHEGLMVKSLSAPYAAGRRGRSWQKIKPTHTLDLLVLGAEWGYGRRTGYLS
HHHCCHHHCCCCCEEEEECCCCHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCC
NLHLGARDPRTGEPVMVGKTFKGLTDALLAWQTAEFPRHERARDEHTVYLWPELVVEIAL
CCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCCHHCCCCCCEEEECHHHHHHHHH
DGVQVSPRYPGGVALRFARVVRYRPDKTPDQADTIDTVRGLLP
CCEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MLFADVVRTSEAVRATRSRKTKVAALAELLRAAESAELAPVVAWLSGELRQGRIGTGWRT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCEE
LTGVRVPPALDAALEVATVDAIFDELAAVSGAGSGNRRKELLTRLWSAATEPEQEFLLRL
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
LTGELRQGALTALVAEAVAAAADVPVEQVRRAYMLSGQLPVTAEAALRGGAAALAEFRLE
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHH
VGRPIQPMLAAPGASLEEAMTEFGGEVSVEHKLDGARIQVHRDGDRIAVFTRTLRDITAG
CCCCCCHHHCCCCCCHHHHHHHCCCCEEEEECCCCCEEEEEECCCEEEHHHHHHHHHHCC
VPELVELVARLDCTSVVLDGETLALTDAGRPRPFQETMSRFATADPARAAEVDLPPGTPV
HHHHHHHHHHCCCCEEEECCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCC
VPPVSSTRELLLHPYFFDCLHLDGRDLLDAPLSERRAALLAVVGEHAIPALIRPEPEAAA
CCCCCCCHHHHHCHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
EYFDGALAAGHEGLMVKSLSAPYAAGRRGRSWQKIKPTHTLDLLVLGAEWGYGRRTGYLS
HHHCCHHHCCCCCEEEEECCCCHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCC
NLHLGARDPRTGEPVMVGKTFKGLTDALLAWQTAEFPRHERARDEHTVYLWPELVVEIAL
CCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCCHHCCCCCCEEEECHHHHHHHHH
DGVQVSPRYPGGVALRFARVVRYRPDKTPDQADTIDTVRGLLP
CCEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA