Click here to switch to the map view.

The map label for this gene is hemDX [H]

Identifier: 54027142

GI number: 54027142

Start: 5465975

End: 5467537

Strand: Reverse

Name: hemDX [H]

Synonym: nfa51680

Alternate gene names: 54027142

Gene position: 5467537-5465975 (Counterclockwise)

Preceding gene: 54027143

Following gene: 54027140

Centisome position: 90.8

GC content: 72.74

Gene sequence:

>1563_bases
ATGAGCCGAGCCACCAAGAAGCACCCCGGTCGGATCCTGTTCGTCGGGTCGGGTCCCGGGGACCCGGCGCTGCTCACCGT
GCGCGCGCGGGAGGTGCTCGGCCGAGCGACACTGGCCTTCACCGATCCCGACGTGGACAAGGGTGTGCTCGCGCTGATCG
GCACGGCGGTGGAGCCGAACGAGGACGGCACCAGCCCGGTCGATGTGCGCCCCGCCCTGGGCGAGCCCGCCGAGGTCGCC
AAGACCCTGATCGCCGAGGCGCGGGGCGGTCACGACGTGGTGCGCGTCGTCGCCGGTGATCCGCTCACCACCGATTCGGT
GATCACCGAGGTCACCGCGGTCACCCGTTCGCACATGGTCTTCGAGGTGCTGCCCGGCCTGCCGAACGGTTCGGCCGTGC
CCAGCTACGCGGGCATCGCGCTCGGCTCCGGCCACACCGAGGCCGATGTGCGCGGCGAGGTCGACTGGGCCGCGCTGGCC
GCCGCGCCCGGCCCGCTGGTCCTGCACGCCACCTCCGGCCACCTCGCCGAGACCGCGAGCGCGCTGGTCGAGCACGGCAT
GGCGCCGCAGACCCCGGTCGCGGTGACCGTGCGCGGCACCACCCGTCAGCAGCGCACCATCGAGGCGACGCTGGCGACCC
TCAACAGCGCGGCCTCCGAGCTGGTCGGCCCGCTGGTGGTGACCATCGGCAAGGTCGTCGCCTCGCGGTCGAAGATGTCG
TGGTGGGAGTCGCGGGCGCTCTACGGCTGGACGGTGCTGGTGCCGCGCACCAAGGATCAGGCCGCCGAGATGAGCGAGCG
GCTGGTCACCCACGGTGCCATCCCGATGGAGGTGCCCACCATCGCCGTCGAGCCGCCGCGCAGCCCCGCTCAGATGGAGC
GCGCGGTGAAGGGCCTGGTCGACGGGCGCTACCAGTGGGTGGTCTTCACCTCCACCAACGCCGTGCGCGCGGTGTGGGAG
AAGTTCGCCGAGTTCGGCCTGGACGCGCGCGCCTTCTCCGGTGTGAAGATCGCGTGCGTCGGCGAGGCCACCGCGGACAA
GGTGCGCTCGTTCGGCATCAACCCCGAACTGGTGCCCTCCGGTGAGCAGTCCTCCGAGGGCCTGCTGGCCGACTTCCCGC
CCTACGACGACGTGTTCGACCCGGTCAACCGGGTGCTGCTGCCGCGCGCGGACATCGCCACCGAGACGCTGGCCGAAGGG
CTGCGCGACCGCGGCTGGGAGATCGACGACGTGACCGCGTACCGGACGGTGCGCGCCTCGCCGCCGCCCGCCGAGACCCG
GGAGATGATCAAGACCGGCGGTTTCGACGCGGTGCTGTTCACCTCCTCGTCCACCGTGCGGAACCTCGTCGGTATCGCGG
GCAAGCCGCACGCGCGCACGATCGTGGCGTGCATCGGGCCCAAGACCGCCGAGACCGCCGTGGAATTCGGCCTGCGGGTC
GACGTGCAGCCCGAGGTCGCCCAGGTGGGCCCGCTGGTCGAGGCACTGGCCGAGCACGCCGCCCGGCTGCGCGCCGAGGG
CCTGCTGCCCCCGCCGCGCAAGAAGAGCCGTCGCAGCCGGTAG

Upstream 100 bases:

>100_bases
TCGAGGTGGCGGCCACGGAGCCGGGCGCGCGCTCCGGGACCGGCGCGGTGCGCCCGCCAGAGACAGACCTCAGCAATCCC
AGTCCGATGGAGAACCCCCA

Downstream 100 bases:

>100_bases
CGAATCAGCGGCTGTAGCGCAGCAGGGTGCGGACCATGCGGCAGGTGGCGTCCGAGGGCGGGCGGATGCCGACGAGTCGT
GCTGTCTCCCTGATGCGCTC

Product: putative uroporphyrin-III C-methyltransferase/uroporphyrinogen-III synthase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Uroporphyrinogen-III synthase; UROS; Hydroxymethylbilane hydrolyase [cyclizing]; Uroporphyrinogen-III cosynthase [H]

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MSRATKKHPGRILFVGSGPGDPALLTVRAREVLGRATLAFTDPDVDKGVLALIGTAVEPNEDGTSPVDVRPALGEPAEVA
KTLIAEARGGHDVVRVVAGDPLTTDSVITEVTAVTRSHMVFEVLPGLPNGSAVPSYAGIALGSGHTEADVRGEVDWAALA
AAPGPLVLHATSGHLAETASALVEHGMAPQTPVAVTVRGTTRQQRTIEATLATLNSAASELVGPLVVTIGKVVASRSKMS
WWESRALYGWTVLVPRTKDQAAEMSERLVTHGAIPMEVPTIAVEPPRSPAQMERAVKGLVDGRYQWVVFTSTNAVRAVWE
KFAEFGLDARAFSGVKIACVGEATADKVRSFGINPELVPSGEQSSEGLLADFPPYDDVFDPVNRVLLPRADIATETLAEG
LRDRGWEIDDVTAYRTVRASPPPAETREMIKTGGFDAVLFTSSSTVRNLVGIAGKPHARTIVACIGPKTAETAVEFGLRV
DVQPEVAQVGPLVEALAEHAARLRAEGLLPPPRKKSRRSR

Sequences:

>Translated_520_residues
MSRATKKHPGRILFVGSGPGDPALLTVRAREVLGRATLAFTDPDVDKGVLALIGTAVEPNEDGTSPVDVRPALGEPAEVA
KTLIAEARGGHDVVRVVAGDPLTTDSVITEVTAVTRSHMVFEVLPGLPNGSAVPSYAGIALGSGHTEADVRGEVDWAALA
AAPGPLVLHATSGHLAETASALVEHGMAPQTPVAVTVRGTTRQQRTIEATLATLNSAASELVGPLVVTIGKVVASRSKMS
WWESRALYGWTVLVPRTKDQAAEMSERLVTHGAIPMEVPTIAVEPPRSPAQMERAVKGLVDGRYQWVVFTSTNAVRAVWE
KFAEFGLDARAFSGVKIACVGEATADKVRSFGINPELVPSGEQSSEGLLADFPPYDDVFDPVNRVLLPRADIATETLAEG
LRDRGWEIDDVTAYRTVRASPPPAETREMIKTGGFDAVLFTSSSTVRNLVGIAGKPHARTIVACIGPKTAETAVEFGLRV
DVQPEVAQVGPLVEALAEHAARLRAEGLLPPPRKKSRRSR
>Mature_519_residues
SRATKKHPGRILFVGSGPGDPALLTVRAREVLGRATLAFTDPDVDKGVLALIGTAVEPNEDGTSPVDVRPALGEPAEVAK
TLIAEARGGHDVVRVVAGDPLTTDSVITEVTAVTRSHMVFEVLPGLPNGSAVPSYAGIALGSGHTEADVRGEVDWAALAA
APGPLVLHATSGHLAETASALVEHGMAPQTPVAVTVRGTTRQQRTIEATLATLNSAASELVGPLVVTIGKVVASRSKMSW
WESRALYGWTVLVPRTKDQAAEMSERLVTHGAIPMEVPTIAVEPPRSPAQMERAVKGLVDGRYQWVVFTSTNAVRAVWEK
FAEFGLDARAFSGVKIACVGEATADKVRSFGINPELVPSGEQSSEGLLADFPPYDDVFDPVNRVLLPRADIATETLAEGL
RDRGWEIDDVTAYRTVRASPPPAETREMIKTGGFDAVLFTSSSTVRNLVGIAGKPHARTIVACIGPKTAETAVEFGLRVD
VQPEVAQVGPLVEALAEHAARLRAEGLLPPPRKKSRRSR

Specific function: May catalyze sequential reactions to synthesize uroporphyrinogen III from hydroxymethylbilane (HMB) and then precorrin-2, which are intermediate compounds in both vitamin B12 and siroheme biosyntheses [H]

COG id: COG1587

COG function: function code H; Uroporphyrinogen-III synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=254, Percent_Identity=29.9212598425197, Blast_Score=118, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR003754
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF02602 HEM4; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =4.2.1.75 [H]

Molecular weight: Translated: 55039; Mature: 54908

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00839 SUMT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRATKKHPGRILFVGSGPGDPALLTVRAREVLGRATLAFTDPDVDKGVLALIGTAVEPN
CCCCCCCCCCEEEEEECCCCCCEEEEEEHHHHHCCEEEEECCCCCCCCHHHHHCCCCCCC
EDGTSPVDVRPALGEPAEVAKTLIAEARGGHDVVRVVAGDPLTTDSVITEVTAVTRSHMV
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHH
FEVLPGLPNGSAVPSYAGIALGSGHTEADVRGEVDWAALAAAPGPLVLHATSGHLAETAS
HHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHCCCCCEEEEECCCCHHHHHH
ALVEHGMAPQTPVAVTVRGTTRQQRTIEATLATLNSAASELVGPLVVTIGKVVASRSKMS
HHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WWESRALYGWTVLVPRTKDQAAEMSERLVTHGAIPMEVPTIAVEPPRSPAQMERAVKGLV
HHHCCCEEEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHH
DGRYQWVVFTSTNAVRAVWEKFAEFGLDARAFSGVKIACVGEATADKVRSFGINPELVPS
CCCEEEEEEECCHHHHHHHHHHHHHCCCCHHCCCEEEEEECCHHHHHHHHCCCCCCCCCC
GEQSSEGLLADFPPYDDVFDPVNRVLLPRADIATETLAEGLRDRGWEIDDVTAYRTVRAS
CCCCCCCCEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECC
PPPAETREMIKTGGFDAVLFTSSSTVRNLVGIAGKPHARTIVACIGPKTAETAVEFGLRV
CCCHHHHHHHHHCCCCEEEEECCHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHCCEE
DVQPEVAQVGPLVEALAEHAARLRAEGLLPPPRKKSRRSR
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCC
>Mature Secondary Structure 
SRATKKHPGRILFVGSGPGDPALLTVRAREVLGRATLAFTDPDVDKGVLALIGTAVEPN
CCCCCCCCCEEEEEECCCCCCEEEEEEHHHHHCCEEEEECCCCCCCCHHHHHCCCCCCC
EDGTSPVDVRPALGEPAEVAKTLIAEARGGHDVVRVVAGDPLTTDSVITEVTAVTRSHMV
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHH
FEVLPGLPNGSAVPSYAGIALGSGHTEADVRGEVDWAALAAAPGPLVLHATSGHLAETAS
HHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHCCCCCEEEEECCCCHHHHHH
ALVEHGMAPQTPVAVTVRGTTRQQRTIEATLATLNSAASELVGPLVVTIGKVVASRSKMS
HHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WWESRALYGWTVLVPRTKDQAAEMSERLVTHGAIPMEVPTIAVEPPRSPAQMERAVKGLV
HHHCCCEEEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHH
DGRYQWVVFTSTNAVRAVWEKFAEFGLDARAFSGVKIACVGEATADKVRSFGINPELVPS
CCCEEEEEEECCHHHHHHHHHHHHHCCCCHHCCCEEEEEECCHHHHHHHHCCCCCCCCCC
GEQSSEGLLADFPPYDDVFDPVNRVLLPRADIATETLAEGLRDRGWEIDDVTAYRTVRAS
CCCCCCCCEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECC
PPPAETREMIKTGGFDAVLFTSSSTVRNLVGIAGKPHARTIVACIGPKTAETAVEFGLRV
CCCHHHHHHHHHCCCCEEEEECCHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHCCEE
DVQPEVAQVGPLVEALAEHAARLRAEGLLPPPRKKSRRSR
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7665501 [H]