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The map label for this gene is hemB [H]

Identifier: 54027140

GI number: 54027140

Start: 5464402

End: 5465379

Strand: Reverse

Name: hemB [H]

Synonym: nfa51660

Alternate gene names: 54027140

Gene position: 5465379-5464402 (Counterclockwise)

Preceding gene: 54027142

Following gene: 54027139

Centisome position: 90.77

GC content: 71.06

Gene sequence:

>978_bases
ATGACCGGAATGGACCGCCCACGGCGGTTGCGCCGTACCCCCGCGTTGCGTCGCCTGGTGGCCGAAACCACAGTGGAGCC
ACGCCATTTGGTGCTGCCCATGTTCGTCGCCGACGGGTTGGACGAACCGCGTGAGATCGGGTCGATGCCCGGCGTGCAGC
AGCACTCGATGGATTCGTTGCGCAAGGCCGCCACGGAGGCGGTCTCGGCGGGGGTCGGCGGTCTGATGCTGTTCGGCGTG
CCACGGCCGGAGGACAAGGACCCGGCGGGCAGCCAGGCCAGCGACCCGGACGGCATCCTGAACCGCGGGCTGCGCGCGCT
GGCCGAGGAGGTCGGCGACGCCACCGTGATCATGGCCGACACCTGTCTCGACGAGTTCACCGACCACGGCCACTGCGGGG
TGCTCGCCGCCGACGGCCACGTCGACAACGACGCCACCCTGCACCGCTATGTGGACATGGCGCTCGCGCAGGCCGAGGCC
GGGGCAGACCTGCTCGGCACCAGCGGCATGATGGACGGCCAGGTCGGCGCGATCCGCCGCGGTCTGGACGCGGTCGGCCG
CACCGACACCGGCATCCTCGCCTACGCCGCCAAATACTCCTCGGCCTTCTACGGGCCGTTCCGGGAAGCGGTGTCGTCCT
CGCTCCAGGGGGACCGCCGCACCTACCAGCAGGACCCGGCCAATCGCCGCGAGTCGATGCACGAGCTGGATCTGGACCTG
GCCGAGGGCGCCGACATCGTGATGGTCAAGCCCGCCATGTCGTACCTGGACATCCTGCGCGAGGTCGCCGACCGTTCCCC
GGTGCCGGTGGCGGCCTACCAGATCTCGGGGGAGTACTCGATGATCACCGCCGCCGCCGAGCGGGGCTGGATCGACCGGC
GCGCCGCCGTGCTCGAGTCGCTGGTCGGCATTCGCCGGGCGGGCGCGGACATCGTGCTGACCTACTGGGCGACCGAGGCC
GCGCACTGGCTGTCGTGA

Upstream 100 bases:

>100_bases
GCGGCTGGCGGTGGGGAACGGCCGCGCGGGCGAATGGGGCGTTCCGCGGCATCCCCGAGGCCGGCGCGACGTCCGTTTTC
CGCAGGCGTATGGTGCGAAT

Downstream 100 bases:

>100_bases
GGTATCCGCCCAGCCCTCTCGGTCCGCCCGCGGAGCCTGCCCCCGCACCGGAAGAGGTGCGCATCGCGACCCAGCTGTGG
TGGGGCGTGGTCGCGGTCGG

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MTGMDRPRRLRRTPALRRLVAETTVEPRHLVLPMFVADGLDEPREIGSMPGVQQHSMDSLRKAATEAVSAGVGGLMLFGV
PRPEDKDPAGSQASDPDGILNRGLRALAEEVGDATVIMADTCLDEFTDHGHCGVLAADGHVDNDATLHRYVDMALAQAEA
GADLLGTSGMMDGQVGAIRRGLDAVGRTDTGILAYAAKYSSAFYGPFREAVSSSLQGDRRTYQQDPANRRESMHELDLDL
AEGADIVMVKPAMSYLDILREVADRSPVPVAAYQISGEYSMITAAAERGWIDRRAAVLESLVGIRRAGADIVLTYWATEA
AHWLS

Sequences:

>Translated_325_residues
MTGMDRPRRLRRTPALRRLVAETTVEPRHLVLPMFVADGLDEPREIGSMPGVQQHSMDSLRKAATEAVSAGVGGLMLFGV
PRPEDKDPAGSQASDPDGILNRGLRALAEEVGDATVIMADTCLDEFTDHGHCGVLAADGHVDNDATLHRYVDMALAQAEA
GADLLGTSGMMDGQVGAIRRGLDAVGRTDTGILAYAAKYSSAFYGPFREAVSSSLQGDRRTYQQDPANRRESMHELDLDL
AEGADIVMVKPAMSYLDILREVADRSPVPVAAYQISGEYSMITAAAERGWIDRRAAVLESLVGIRRAGADIVLTYWATEA
AHWLS
>Mature_324_residues
TGMDRPRRLRRTPALRRLVAETTVEPRHLVLPMFVADGLDEPREIGSMPGVQQHSMDSLRKAATEAVSAGVGGLMLFGVP
RPEDKDPAGSQASDPDGILNRGLRALAEEVGDATVIMADTCLDEFTDHGHCGVLAADGHVDNDATLHRYVDMALAQAEAG
ADLLGTSGMMDGQVGAIRRGLDAVGRTDTGILAYAAKYSSAFYGPFREAVSSSLQGDRRTYQQDPANRRESMHELDLDLA
EGADIVMVKPAMSYLDILREVADRSPVPVAAYQISGEYSMITAAAERGWIDRRAAVLESLVGIRRAGADIVLTYWATEAA
HWLS

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=308, Percent_Identity=39.9350649350649, Blast_Score=231, Evalue=9e-61,
Organism=Escherichia coli, GI87081728, Length=319, Percent_Identity=47.9623824451411, Blast_Score=290, Evalue=1e-79,
Organism=Saccharomyces cerevisiae, GI6321398, Length=315, Percent_Identity=39.6825396825397, Blast_Score=234, Evalue=2e-62,
Organism=Drosophila melanogaster, GI21358291, Length=294, Percent_Identity=39.7959183673469, Blast_Score=221, Evalue=6e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 35021; Mature: 34890

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGMDRPRRLRRTPALRRLVAETTVEPRHLVLPMFVADGLDEPREIGSMPGVQQHSMDSL
CCCCCCHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHH
RKAATEAVSAGVGGLMLFGVPRPEDKDPAGSQASDPDGILNRGLRALAEEVGDATVIMAD
HHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEHH
TCLDEFTDHGHCGVLAADGHVDNDATLHRYVDMALAQAEAGADLLGTSGMMDGQVGAIRR
HHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHH
GLDAVGRTDTGILAYAAKYSSAFYGPFREAVSSSLQGDRRTYQQDPANRRESMHELDLDL
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHCCHH
AEGADIVMVKPAMSYLDILREVADRSPVPVAAYQISGEYSMITAAAERGWIDRRAAVLES
HCCCCEEEECHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCHHHHHHHHHH
LVGIRRAGADIVLTYWATEAAHWLS
HHHHHHCCCCEEEEEEHHHHHHHCC
>Mature Secondary Structure 
TGMDRPRRLRRTPALRRLVAETTVEPRHLVLPMFVADGLDEPREIGSMPGVQQHSMDSL
CCCCCHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHH
RKAATEAVSAGVGGLMLFGVPRPEDKDPAGSQASDPDGILNRGLRALAEEVGDATVIMAD
HHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEHH
TCLDEFTDHGHCGVLAADGHVDNDATLHRYVDMALAQAEAGADLLGTSGMMDGQVGAIRR
HHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHH
GLDAVGRTDTGILAYAAKYSSAFYGPFREAVSSSLQGDRRTYQQDPANRRESMHELDLDL
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHCCHH
AEGADIVMVKPAMSYLDILREVADRSPVPVAAYQISGEYSMITAAAERGWIDRRAAVLES
HCCCCEEEECHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCHHHHHHHHHH
LVGIRRAGADIVLTYWATEAAHWLS
HHHHHHCCCCEEEEEEHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]