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The map label for this gene is pgk

Identifier: 54025558

GI number: 54025558

Start: 3798072

End: 3799304

Strand: Reverse

Name: pgk

Synonym: nfa35880

Alternate gene names: 54025558

Gene position: 3799304-3798072 (Counterclockwise)

Preceding gene: 54025559

Following gene: 54025557

Centisome position: 63.1

GC content: 69.83

Gene sequence:

>1233_bases
ATGGCTGTCAAGACACTCCAGGATCTCCTGAACGAGGGTGTCGAAGGTCGGGGCGTGCTGGTGCGCTCCGACCTGAACGT
CCCCCTCGACGACAACGGGCAGATCACCGATCCCGGCCGCATCATCGCCTCGATCCCGACGCTGAAGGCCCTGGTCGAGG
CGGGCGCCAAGGTGGTCGTCACCGCGCACCTCGGCCGTCCCAAGGGCGAGCCGGACCCGAAGTTCTCGCTCGCGCCGGTG
GCGGCGAAGCTGGCCGAACTGCTCGGCCGCAACGTGCAGCTGGCCGGTGACGTGGTGGGCTACGACGCGCTCTCGCGCTC
GGAGGGCCTCACCGACGGTGACGTGCTGCTGCTGGAGAACGTGCGTTTCGATCCGCGCGAGACCAGCAAGGACGAGGCCG
AGCGCGGCAAGCTCGCGGCGGCCCTGGTCGAGCTGGTCGGCGAGGACGGCGCGTTCGTCTCCGACGGTTTCGGCGTGGTG
CATCGCAAGCAGGCCTCGGTCTACGACGTGGCCAAGTTGCTGCCGCACTACGCGGGCACGCTGGTCGCCGCCGAGGTGGA
CGTGCTGGCCAAGCTCACCGACAACACCGAGCGGCCCTACGCCGTGGTGCTGGGCGGCTCGAAGGTCTCCGACAAGCTCG
CCGTCATCGAGGCGCTGGCGCCCAAGGTCGACACCCTGGTGATCGGCGGCGGCATGTGCTTCACCTTCCTTGCCGCACAG
GGCCTTTCGGTCGGCTCCTCGCTGCTGCAGGAGGAGATGATCGACACCTGCAAGGGGCTGCTCGAGCGGTACGCCGACGT
CATCCACCTGCCGCGCGACGTGGTGGTCGCCGACTCGTTCTCCGCCGACGCGGAGTCGAAGTGCGTGCCGGCCAACGAGA
TCCCGGACGGCTGGATGGGTCTGGACATCGGCGCGGAGTCGGTGGACCGGTTCGCGGCGCTGCTGACCGAGGCCAAGACG
GTGTTCTGGAACGGCCCGATGGGCGTGTTCGAGTTCGAGAAGTTCGCCGCGGGCACCCGCGGGGTCGCCGAGGCGATCGT
GGCGGCCACCGGCAAGGGGGCGTTCACCGTCGTCGGCGGCGGCGACTCGGCCGCGGCGGTCCGCGCGCTCGGCCTGCCCG
AGGACGGTTTCTCGCACATCTCCACCGGCGGCGGCGCGTCGCTGGAATACCTGGAGGGCAAGGAACTTCCCGGCATCAGT
GTCCTCGAGGACACCGCTCCGGAGGGCAGCTGA

Upstream 100 bases:

>100_bases
GACGATCAGGTGAAGGTCTACTCCTGGTACGACAACGAGTGGGGCTACTCCAACCGCCTCGCCGACCTCATCGGCCTCGT
CGGCAAGTCCCTCTGATCAC

Downstream 100 bases:

>100_bases
ATGGCACGTAAACCGCTCATCGCCGGCAACTGGAAGATGAACCTCAACCATCTCGAGGCCATCGCCCTGGTGCAGAAGAT
CGCCTTCGCGCTGCCGGAGA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MAVKTLQDLLNEGVEGRGVLVRSDLNVPLDDNGQITDPGRIIASIPTLKALVEAGAKVVVTAHLGRPKGEPDPKFSLAPV
AAKLAELLGRNVQLAGDVVGYDALSRSEGLTDGDVLLLENVRFDPRETSKDEAERGKLAAALVELVGEDGAFVSDGFGVV
HRKQASVYDVAKLLPHYAGTLVAAEVDVLAKLTDNTERPYAVVLGGSKVSDKLAVIEALAPKVDTLVIGGGMCFTFLAAQ
GLSVGSSLLQEEMIDTCKGLLERYADVIHLPRDVVVADSFSADAESKCVPANEIPDGWMGLDIGAESVDRFAALLTEAKT
VFWNGPMGVFEFEKFAAGTRGVAEAIVAATGKGAFTVVGGGDSAAAVRALGLPEDGFSHISTGGGASLEYLEGKELPGIS
VLEDTAPEGS

Sequences:

>Translated_410_residues
MAVKTLQDLLNEGVEGRGVLVRSDLNVPLDDNGQITDPGRIIASIPTLKALVEAGAKVVVTAHLGRPKGEPDPKFSLAPV
AAKLAELLGRNVQLAGDVVGYDALSRSEGLTDGDVLLLENVRFDPRETSKDEAERGKLAAALVELVGEDGAFVSDGFGVV
HRKQASVYDVAKLLPHYAGTLVAAEVDVLAKLTDNTERPYAVVLGGSKVSDKLAVIEALAPKVDTLVIGGGMCFTFLAAQ
GLSVGSSLLQEEMIDTCKGLLERYADVIHLPRDVVVADSFSADAESKCVPANEIPDGWMGLDIGAESVDRFAALLTEAKT
VFWNGPMGVFEFEKFAAGTRGVAEAIVAATGKGAFTVVGGGDSAAAVRALGLPEDGFSHISTGGGASLEYLEGKELPGIS
VLEDTAPEGS
>Mature_409_residues
AVKTLQDLLNEGVEGRGVLVRSDLNVPLDDNGQITDPGRIIASIPTLKALVEAGAKVVVTAHLGRPKGEPDPKFSLAPVA
AKLAELLGRNVQLAGDVVGYDALSRSEGLTDGDVLLLENVRFDPRETSKDEAERGKLAAALVELVGEDGAFVSDGFGVVH
RKQASVYDVAKLLPHYAGTLVAAEVDVLAKLTDNTERPYAVVLGGSKVSDKLAVIEALAPKVDTLVIGGGMCFTFLAAQG
LSVGSSLLQEEMIDTCKGLLERYADVIHLPRDVVVADSFSADAESKCVPANEIPDGWMGLDIGAESVDRFAALLTEAKTV
FWNGPMGVFEFEKFAAGTRGVAEAIVAATGKGAFTVVGGGDSAAAVRALGLPEDGFSHISTGGGASLEYLEGKELPGISV
LEDTAPEGS

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=407, Percent_Identity=42.7518427518428, Blast_Score=297, Evalue=1e-80,
Organism=Homo sapiens, GI31543397, Length=407, Percent_Identity=42.7518427518428, Blast_Score=293, Evalue=2e-79,
Organism=Escherichia coli, GI1789294, Length=411, Percent_Identity=42.3357664233577, Blast_Score=275, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI17508823, Length=412, Percent_Identity=44.4174757281553, Blast_Score=305, Evalue=2e-83,
Organism=Saccharomyces cerevisiae, GI6319857, Length=415, Percent_Identity=41.4457831325301, Blast_Score=289, Evalue=5e-79,
Organism=Drosophila melanogaster, GI17136394, Length=409, Percent_Identity=40.0977995110024, Blast_Score=268, Evalue=4e-72,
Organism=Drosophila melanogaster, GI24581203, Length=427, Percent_Identity=40.5152224824356, Blast_Score=264, Evalue=8e-71,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_NOCFA (Q5YTQ5)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_119800.1
- HSSP:   P36204
- ProteinModelPortal:   Q5YTQ5
- SMR:   Q5YTQ5
- GeneID:   3111366
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa35880
- NMPDR:   fig|247156.1.peg.3502
- HOGENOM:   HBG453500
- OMA:   LSYFSKA
- ProtClustDB:   PRK00073
- BioCyc:   NFAR247156:NFA35880-MONOMER
- BRENDA:   2.7.2.3
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 42609; Mature: 42478

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 40-40 BINDING 122-122 BINDING 162-162 BINDING 212-212 BINDING 300-300 BINDING 331-331

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVKTLQDLLNEGVEGRGVLVRSDLNVPLDDNGQITDPGRIIASIPTLKALVEAGAKVVV
CCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCEEEE
TAHLGRPKGEPDPKFSLAPVAAKLAELLGRNVQLAGDVVGYDALSRSEGLTDGDVLLLEN
EEECCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCCCCEEEEEC
VRFDPRETSKDEAERGKLAAALVELVGEDGAFVSDGFGVVHRKQASVYDVAKLLPHYAGT
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEECCCHHHHHHHHHHHHHHH
LVAAEVDVLAKLTDNTERPYAVVLGGSKVSDKLAVIEALAPKVDTLVIGGGMCFTFLAAQ
HHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHC
GLSVGSSLLQEEMIDTCKGLLERYADVIHLPRDVVVADSFSADAESKCVPANEIPDGWMG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCEE
LDIGAESVDRFAALLTEAKTVFWNGPMGVFEFEKFAAGTRGVAEAIVAATGKGAFTVVGG
CCCCHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEC
GDSAAAVRALGLPEDGFSHISTGGGASLEYLEGKELPGISVLEDTAPEGS
CCCHHHHEECCCCCCCHHHCCCCCCCEEEECCCCCCCCCEEECCCCCCCC
>Mature Secondary Structure 
AVKTLQDLLNEGVEGRGVLVRSDLNVPLDDNGQITDPGRIIASIPTLKALVEAGAKVVV
CHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCEEEE
TAHLGRPKGEPDPKFSLAPVAAKLAELLGRNVQLAGDVVGYDALSRSEGLTDGDVLLLEN
EEECCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCCCCEEEEEC
VRFDPRETSKDEAERGKLAAALVELVGEDGAFVSDGFGVVHRKQASVYDVAKLLPHYAGT
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEECCCHHHHHHHHHHHHHHH
LVAAEVDVLAKLTDNTERPYAVVLGGSKVSDKLAVIEALAPKVDTLVIGGGMCFTFLAAQ
HHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHC
GLSVGSSLLQEEMIDTCKGLLERYADVIHLPRDVVVADSFSADAESKCVPANEIPDGWMG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCEE
LDIGAESVDRFAALLTEAKTVFWNGPMGVFEFEKFAAGTRGVAEAIVAATGKGAFTVVGG
CCCCHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEC
GDSAAAVRALGLPEDGFSHISTGGGASLEYLEGKELPGISVLEDTAPEGS
CCCHHHHEECCCCCCCHHHCCCCCCCEEEECCCCCCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA