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The map label for this gene is tpiA

Identifier: 54025557

GI number: 54025557

Start: 3797286

End: 3798071

Strand: Reverse

Name: tpiA

Synonym: nfa35870

Alternate gene names: 54025557

Gene position: 3798071-3797286 (Counterclockwise)

Preceding gene: 54025558

Following gene: 54025555

Centisome position: 63.08

GC content: 69.21

Gene sequence:

>786_bases
ATGGCACGTAAACCGCTCATCGCCGGCAACTGGAAGATGAACCTCAACCATCTCGAGGCCATCGCCCTGGTGCAGAAGAT
CGCCTTCGCGCTGCCGGAGAAGTACTTCGAGAAGGTCGACGTCGCGGTGATCCCGCCGTTCGTGGACATCCGCAGCGTGC
AGACGCTGGTGGAGGGCGACAAGCTGCTGCTCACCTACGGCGCGCAGGACGTCTCGGTGCACGAGTCCGGCGCCTACACC
GGCGAGATCAGCGCGAGCATGCTCGCCAAGCTGGGCTGCACCTTCGTGGTGGTCGGGCACTCCGAGCGGCGCCAGTACCA
CCACGAGGACGACGCCACCGTGCTCGGCAAGGCCAAGAAGGCCCTCGAGCACGGCCTCACCCCGATCGTGTGCATCGGTG
AGGGGCTCAACGTCCGCGAGGCGGGCACGCACGTGGAATACAACCTCGAGCAGCTGCGCGGGTCGCTGAAGGGCCTGTCC
GCCGAGCAGATCGCGAAGGTCGTCATCGCCTACGAGCCGGTCTGGGCCATCGGCACCGGCAAGGTGGCGAGCGCGGCCGA
CGCGCAGGAGGTGTGCGGCGCCATCCGCGCCGAGCTGGCCGAGCTGGCCGGACCCGAGGTGGCCGCGCAGGTGCGGGTGC
TCTACGGCGGCTCGGTGAACGCCAAGAACGTCGGGGAACTGGTCGCCCAGCCCGATGTCGACGGTGCGCTGGTCGGCGGG
GCCTCGCTCAAGGGTGACGAGTTCGCCACCCTGTCCGCGATCGCCGCGGGCGGCCCGCTGCCCTGA

Upstream 100 bases:

>100_bases
GCACATCTCCACCGGCGGCGGCGCGTCGCTGGAATACCTGGAGGGCAAGGAACTTCCCGGCATCAGTGTCCTCGAGGACA
CCGCTCCGGAGGGCAGCTGA

Downstream 100 bases:

>100_bases
TTGCGGTCCCGGCATCGAACTCCGGCCCGGCGTCGCCTCCGCGATGCCGGGCCGGTTCGTTTCCGCGCCGGTTCGTTTCC
GCGCCGGTTCGTTCGGGAGC

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MARKPLIAGNWKMNLNHLEAIALVQKIAFALPEKYFEKVDVAVIPPFVDIRSVQTLVEGDKLLLTYGAQDVSVHESGAYT
GEISASMLAKLGCTFVVVGHSERRQYHHEDDATVLGKAKKALEHGLTPIVCIGEGLNVREAGTHVEYNLEQLRGSLKGLS
AEQIAKVVIAYEPVWAIGTGKVASAADAQEVCGAIRAELAELAGPEVAAQVRVLYGGSVNAKNVGELVAQPDVDGALVGG
ASLKGDEFATLSAIAAGGPLP

Sequences:

>Translated_261_residues
MARKPLIAGNWKMNLNHLEAIALVQKIAFALPEKYFEKVDVAVIPPFVDIRSVQTLVEGDKLLLTYGAQDVSVHESGAYT
GEISASMLAKLGCTFVVVGHSERRQYHHEDDATVLGKAKKALEHGLTPIVCIGEGLNVREAGTHVEYNLEQLRGSLKGLS
AEQIAKVVIAYEPVWAIGTGKVASAADAQEVCGAIRAELAELAGPEVAAQVRVLYGGSVNAKNVGELVAQPDVDGALVGG
ASLKGDEFATLSAIAAGGPLP
>Mature_260_residues
ARKPLIAGNWKMNLNHLEAIALVQKIAFALPEKYFEKVDVAVIPPFVDIRSVQTLVEGDKLLLTYGAQDVSVHESGAYTG
EISASMLAKLGCTFVVVGHSERRQYHHEDDATVLGKAKKALEHGLTPIVCIGEGLNVREAGTHVEYNLEQLRGSLKGLSA
EQIAKVVIAYEPVWAIGTGKVASAADAQEVCGAIRAELAELAGPEVAAQVRVLYGGSVNAKNVGELVAQPDVDGALVGGA
SLKGDEFATLSAIAAGGPLP

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=243, Percent_Identity=41.1522633744856, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI226529917, Length=243, Percent_Identity=41.1522633744856, Blast_Score=157, Evalue=1e-38,
Organism=Escherichia coli, GI1790353, Length=249, Percent_Identity=46.1847389558233, Blast_Score=196, Evalue=9e-52,
Organism=Caenorhabditis elegans, GI17536593, Length=254, Percent_Identity=44.8818897637795, Blast_Score=183, Evalue=7e-47,
Organism=Saccharomyces cerevisiae, GI6320255, Length=245, Percent_Identity=41.2244897959184, Blast_Score=171, Evalue=9e-44,
Organism=Drosophila melanogaster, GI28572008, Length=244, Percent_Identity=40.1639344262295, Blast_Score=160, Evalue=5e-40,
Organism=Drosophila melanogaster, GI28572006, Length=244, Percent_Identity=40.1639344262295, Blast_Score=160, Evalue=5e-40,
Organism=Drosophila melanogaster, GI28572004, Length=244, Percent_Identity=40.1639344262295, Blast_Score=160, Evalue=6e-40,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_NOCFA (Q5YTQ6)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_119799.1
- HSSP:   P0A858
- ProteinModelPortal:   Q5YTQ6
- GeneID:   3111604
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa35870
- NMPDR:   fig|247156.1.peg.3501
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK00042
- BioCyc:   NFAR247156:NFA35870-MONOMER
- BRENDA:   5.3.1.1
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 27416; Mature: 27285

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 100-100 ACT_SITE 172-172 BINDING 10-10 BINDING 12-12

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKPLIAGNWKMNLNHLEAIALVQKIAFALPEKYFEKVDVAVIPPFVDIRSVQTLVEGD
CCCCCEEECCEEECHHHHHHHHHHHHHHHHCCHHHHHHCCEEEECCCHHHHHHHHHHCCC
KLLLTYGAQDVSVHESGAYTGEISASMLAKLGCTFVVVGHSERRQYHHEDDATVLGKAKK
EEEEEECCCEEEEECCCCCCCHHHHHHHHHCCCEEEEEECHHHHHHCCCCCHHHHHHHHH
ALEHGLTPIVCIGEGLNVREAGTHVEYNLEQLRGSLKGLSAEQIAKVVIAYEPVWAIGTG
HHHHCCCEEEEECCCCCCCCCCCCEEEEHHHHHHHHCCCCHHHHHHHHHEECCEEEECCC
KVASAADAQEVCGAIRAELAELAGPEVAAQVRVLYGGSVNAKNVGELVAQPDVDGALVGG
CCCCCCCHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCCCCCHHHHHHCCCCCCCEEECC
ASLKGDEFATLSAIAAGGPLP
CCCCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
ARKPLIAGNWKMNLNHLEAIALVQKIAFALPEKYFEKVDVAVIPPFVDIRSVQTLVEGD
CCCCEEECCEEECHHHHHHHHHHHHHHHHCCHHHHHHCCEEEECCCHHHHHHHHHHCCC
KLLLTYGAQDVSVHESGAYTGEISASMLAKLGCTFVVVGHSERRQYHHEDDATVLGKAKK
EEEEEECCCEEEEECCCCCCCHHHHHHHHHCCCEEEEEECHHHHHHCCCCCHHHHHHHHH
ALEHGLTPIVCIGEGLNVREAGTHVEYNLEQLRGSLKGLSAEQIAKVVIAYEPVWAIGTG
HHHHCCCEEEEECCCCCCCCCCCCEEEEHHHHHHHHCCCCHHHHHHHHHEECCEEEECCC
KVASAADAQEVCGAIRAELAELAGPEVAAQVRVLYGGSVNAKNVGELVAQPDVDGALVGG
CCCCCCCHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCCCCCHHHHHHCCCCCCCEEECC
ASLKGDEFATLSAIAAGGPLP
CCCCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA