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The map label for this gene is grpE

Identifier: 54019980

GI number: 54019980

Start: 12101

End: 12847

Strand: Direct

Name: grpE

Synonym: mhp011

Alternate gene names: 54019980

Gene position: 12101-12847 (Clockwise)

Preceding gene: 54019979

Following gene: 54019981

Centisome position: 1.36

GC content: 27.18

Gene sequence:

>747_bases
ATGGTATTTGGTGATAAAAAATTACAAGAATTTGAAAATAATTTGCAAAATTTGTCTTCTGATAATGATTTAGAAGACAA
AAAAGTTATAAATCCTGATGAAAAACAAGAGCTCGAAATAGATAAAGAAGTAGAAAAAACTGAGAAAACTGAGAAAAAAA
CTCGTAGATTTTTGAAAAAAGAAGCAAAATTAAAAGACCTTGAAAATCAAATTCAAAATTTAACCACTAGAAATATAACT
CTCGAAATCGAAGCAATTAAGTTAAAAGATAAGATCAAAAAGTTAGAGGAAGATTTTAAATCTCAGGTTAAAACTTTTGA
AGAAAAAGCAACACAAAAAGTCAAAGAATTAAAACTTGATTTACAAAAAAAGCTCGAAAATGAGCAGGATTTACTTAAAA
AATACAGTTTACAACCATTTTTTGAGGATTTTAGCTCGCCTTTTTTAAACCTAAAAAAAGCTATTTCTTATGGTTTAATC
TCTCAAAATCCCGAAATTTCGGCGTATGTAAAAGGTTTTGAAATGCTTGTAAATCAAATTGAAAATGTAATGGAAAATTT
CGGTTTAGTCAAAATATATCCTAAAATTGGTGATTTTTTTGACTCATCAGTCCACGAAATTTATGAAATAAAAACAGGGG
AAAATGATAAAATCATTGAAGTTGTTTCAGAAGGTTATAAATTACACGACCGAATTGTGAAAACAGCGCTTGTTGTAGTT
GGAAAACCTAATGAAGAAAAAAATTAG

Upstream 100 bases:

>100_bases
TTGGGGCAGCTAAAAAAATTAATTATTCGGCCGCTCGAACAGGTATAAAACTTTTGGAAGATTTTTTATCAAACAAAAGC
AAGATTAGAAAGGGATAAAA

Downstream 100 bases:

>100_bases
CCAGGCAATAATTAAATTTTTTAAAAATGAAAATTTAATTATTGATGAAAGTAAACTAATTATTGAAAAGTCAAAAAATT
TTGGTGATTATAGCTCTAAT

Product: heat shock protein

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MVFGDKKLQEFENNLQNLSSDNDLEDKKVINPDEKQELEIDKEVEKTEKTEKKTRRFLKKEAKLKDLENQIQNLTTRNIT
LEIEAIKLKDKIKKLEEDFKSQVKTFEEKATQKVKELKLDLQKKLENEQDLLKKYSLQPFFEDFSSPFLNLKKAISYGLI
SQNPEISAYVKGFEMLVNQIENVMENFGLVKIYPKIGDFFDSSVHEIYEIKTGENDKIIEVVSEGYKLHDRIVKTALVVV
GKPNEEKN

Sequences:

>Translated_248_residues
MVFGDKKLQEFENNLQNLSSDNDLEDKKVINPDEKQELEIDKEVEKTEKTEKKTRRFLKKEAKLKDLENQIQNLTTRNIT
LEIEAIKLKDKIKKLEEDFKSQVKTFEEKATQKVKELKLDLQKKLENEQDLLKKYSLQPFFEDFSSPFLNLKKAISYGLI
SQNPEISAYVKGFEMLVNQIENVMENFGLVKIYPKIGDFFDSSVHEIYEIKTGENDKIIEVVSEGYKLHDRIVKTALVVV
GKPNEEKN
>Mature_248_residues
MVFGDKKLQEFENNLQNLSSDNDLEDKKVINPDEKQELEIDKEVEKTEKTEKKTRRFLKKEAKLKDLENQIQNLTTRNIT
LEIEAIKLKDKIKKLEEDFKSQVKTFEEKATQKVKELKLDLQKKLENEQDLLKKYSLQPFFEDFSSPFLNLKKAISYGLI
SQNPEISAYVKGFEMLVNQIENVMENFGLVKIYPKIGDFFDSSVHEIYEIKTGENDKIIEVVSEGYKLHDRIVKTALVVV
GKPNEEKN

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

None

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 28997; Mature: 28997

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFGDKKLQEFENNLQNLSSDNDLEDKKVINPDEKQELEIDKEVEKTEKTEKKTRRFLKK
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EAKLKDLENQIQNLTTRNITLEIEAIKLKDKIKKLEEDFKSQVKTFEEKATQKVKELKLD
HHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQKKLENEQDLLKKYSLQPFFEDFSSPFLNLKKAISYGLISQNPEISAYVKGFEMLVNQI
HHHHHCCHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
ENVMENFGLVKIYPKIGDFFDSSVHEIYEIKTGENDKIIEVVSEGYKLHDRIVKTALVVV
HHHHHHCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHE
GKPNEEKN
ECCCCCCC
>Mature Secondary Structure
MVFGDKKLQEFENNLQNLSSDNDLEDKKVINPDEKQELEIDKEVEKTEKTEKKTRRFLKK
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EAKLKDLENQIQNLTTRNITLEIEAIKLKDKIKKLEEDFKSQVKTFEEKATQKVKELKLD
HHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQKKLENEQDLLKKYSLQPFFEDFSSPFLNLKKAISYGLISQNPEISAYVKGFEMLVNQI
HHHHHCCHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
ENVMENFGLVKIYPKIGDFFDSSVHEIYEIKTGENDKIIEVVSEGYKLHDRIVKTALVVV
HHHHHHCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHE
GKPNEEKN
ECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA