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The map label for this gene is rpoD [H]

Identifier: 53722776

GI number: 53722776

Start: 2406884

End: 2408926

Strand: Reverse

Name: rpoD [H]

Synonym: BPSS1755

Alternate gene names: 53722776

Gene position: 2408926-2406884 (Counterclockwise)

Preceding gene: 53722777

Following gene: 53722773

Centisome position: 75.92

GC content: 63.93

Gene sequence:

>2043_bases
ATGACGAAAAAGCTGAACGAAGTATCCGTCGATGACGACGCAAACCAGAGCGACGACCAGCCTACCGCTGCGTCGGGCAA
AGGCGAAAAGGCCAAGGCGCGCGACCGTCGCGCCAAGGAAAAGGCGCTGCTGAAGGAAGCGTTCGCGACGAGCACGCCCG
GCACGGCCGAGGAGCTCGAAGAGCGCCGTACGAAGCTGCGCGCGCTGATCAAGCTCGGCAAGGAGCGCGGCTTCCTCACG
TACGCCGAAATCAACGACCATCTGCCGGACAACTTCACGGAAACGGAAGCGCTCGAAGGCATCATCGGCACGTTCAACGA
CATGGGCGTCGCGGTGTACGAGCAGGCGCCCGACGCCGAGACGCTGCTGTTGAACGACAATGCGCCCGCCGCGTCGTCCG
ACGATGAAGTCGAGGAGGAAGCGGAAGTCGCGCTGTCGACCGTCGATTCCGAATTCGGCCGCACGACCGATCCGGTGCGG
ATGTACATGCGCGAGATGGGCACGGTCGAGCTGCTCACGCGCGAAGGCGAAATCGAGATCGCGAAGCGGATCGAGGACGG
CCTGCGCCACATGGTGATGGCGATTTCCGCGTGCCCGACGACGATCGCCGACATCCTCGCGATGGCCGAGCGCGTGGCGA
ACGAGGAGAGCCGCGTCGACGAGCTCGTCGACGGCCTGATCGATCCGAACGCCGCCGATACCGACGGCTTCTCGGAGAAG
GACGCCGAGGAAATCGAGAACGAGGACGCGGAAGCCGAAGAGGACGACGAAGAGGAAGAAGAGGACGACGACGGCGCCGC
GCAGGCGACCGCCAACGCGGCCCAGCTCGAAGCGCTCAAGCGCGCGTCGCTCGAGAAGTTCGCGCTCATCAGCGAATGGT
TCGACAAGATGCGCCGCGCGTTCGAGAAGGAAGGCTACAAGTCGAAGTCGTACCTGAAGGCACAGGAAACGATCCAGAAC
GAGCTGATGTCGATCCGCTTCACCGCGCGCACCGTCGAGCGCCTGTGCGATACGCTGCGCGCGCAGGTCGACGAAGTGCG
CCAGGTCGAGCGTCAGATCCTGCACATCGTCGTCGACAAGTGCGGGATGCCGCGCTCGGAGTTCATCGCGCGCTTCCCGG
GCAGCGAGACCGATCTCGACTGGGCCGAGAAGGTCGCGGCCGAAGGCCATTCGTATGGGGCGATCCTGTCGCGCAACATT
CCGGCGATCCGCGAACAGCAGCAGCGCCTGCTCGACCTGCAGGCGCGCGTCGTGCTGCCGCTCAAGGACCTGAAGGAAAC
CAACCGCCAGATGGCGGCGGGCGAACTGAAGGCGCGCCAGGCGAAGCGCGAGATGACCGAGGCGAACCTGCGCCTCGTGA
TCTCGATCGCGAAGAAGTACACGAACCGCGGCCTGCAGTTCCTGGACCTGATTCAGGAGGGCAACATCGGCCTGATGAAG
GCGGTGGACAAGTTCGAATACCGTCGCGGCTACAAGTTCTCGACGTATGCGACGTGGTGGATCCGCCAGGCCATCACGCG
CTCGATCGCGGACCAGGCGCGCACGATCCGGATTCCGGTTCACATGATCGAGACGATCAACAAGATGAACCGCATCTCGC
GGCAGATCCTGCAGGAAACCGGCCTCGAGCCGGATCCGGCGACGCTCGCCGAGAAGATGGAGATGCCGGAGGACAAGATC
CGCAAGATCATGAAGATCGCGAAGGAGCCGATCTCGATGGAAACGCCGATCGGCGACGACGACGATTCCCATCTCGGCGA
CTTCATCGAGGACACCAACACGGTCGCGCCGGCGGATGCCGCGCTGCATGCGAGCATGCGCGACGTCGTGAAGGACGTGC
TCGATTCGCTGACGCCGCGCGAGGCGAAGGTGCTGCGGATGCGCTTCGGCATCGAGATGAGCACCGATCACACGCTCGAG
GAAGTCGGCAAGCAGTTCGACGTCACGCGCGAGCGGATTCGTCAGATCGAGGCGAAGGCGCTGCGCAAGCTGCGTCATCC
GAGCCGTTCGGACAAGCTGAAGTCGTTCCTCGAGGGGAACTGA

Upstream 100 bases:

>100_bases
GGCTGCCGCGCCGGACGAAACGGCAGCTACTCGAGCATCCACGGTTCAACCGGCTGTAGTCCAGCAGCCGCGAGTCGATT
TAGCCGGTACGGCGAACTCC

Downstream 100 bases:

>100_bases
TCGTCCGATTCCGGTCGATCGCCGCCGGGGCGCAAAAATCCGGCGGCGATTCCCTTACTTAGTTGTTACAATGCCGACCC
GGCCAGCATTACCGGGTCTT

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-70 [H]

Number of amino acids: Translated: 680; Mature: 679

Protein sequence:

>680_residues
MTKKLNEVSVDDDANQSDDQPTAASGKGEKAKARDRRAKEKALLKEAFATSTPGTAEELEERRTKLRALIKLGKERGFLT
YAEINDHLPDNFTETEALEGIIGTFNDMGVAVYEQAPDAETLLLNDNAPAASSDDEVEEEAEVALSTVDSEFGRTTDPVR
MYMREMGTVELLTREGEIEIAKRIEDGLRHMVMAISACPTTIADILAMAERVANEESRVDELVDGLIDPNAADTDGFSEK
DAEEIENEDAEAEEDDEEEEEDDDGAAQATANAAQLEALKRASLEKFALISEWFDKMRRAFEKEGYKSKSYLKAQETIQN
ELMSIRFTARTVERLCDTLRAQVDEVRQVERQILHIVVDKCGMPRSEFIARFPGSETDLDWAEKVAAEGHSYGAILSRNI
PAIREQQQRLLDLQARVVLPLKDLKETNRQMAAGELKARQAKREMTEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMK
AVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQILQETGLEPDPATLAEKMEMPEDKI
RKIMKIAKEPISMETPIGDDDDSHLGDFIEDTNTVAPADAALHASMRDVVKDVLDSLTPREAKVLRMRFGIEMSTDHTLE
EVGKQFDVTRERIRQIEAKALRKLRHPSRSDKLKSFLEGN

Sequences:

>Translated_680_residues
MTKKLNEVSVDDDANQSDDQPTAASGKGEKAKARDRRAKEKALLKEAFATSTPGTAEELEERRTKLRALIKLGKERGFLT
YAEINDHLPDNFTETEALEGIIGTFNDMGVAVYEQAPDAETLLLNDNAPAASSDDEVEEEAEVALSTVDSEFGRTTDPVR
MYMREMGTVELLTREGEIEIAKRIEDGLRHMVMAISACPTTIADILAMAERVANEESRVDELVDGLIDPNAADTDGFSEK
DAEEIENEDAEAEEDDEEEEEDDDGAAQATANAAQLEALKRASLEKFALISEWFDKMRRAFEKEGYKSKSYLKAQETIQN
ELMSIRFTARTVERLCDTLRAQVDEVRQVERQILHIVVDKCGMPRSEFIARFPGSETDLDWAEKVAAEGHSYGAILSRNI
PAIREQQQRLLDLQARVVLPLKDLKETNRQMAAGELKARQAKREMTEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMK
AVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQILQETGLEPDPATLAEKMEMPEDKI
RKIMKIAKEPISMETPIGDDDDSHLGDFIEDTNTVAPADAALHASMRDVVKDVLDSLTPREAKVLRMRFGIEMSTDHTLE
EVGKQFDVTRERIRQIEAKALRKLRHPSRSDKLKSFLEGN
>Mature_679_residues
TKKLNEVSVDDDANQSDDQPTAASGKGEKAKARDRRAKEKALLKEAFATSTPGTAEELEERRTKLRALIKLGKERGFLTY
AEINDHLPDNFTETEALEGIIGTFNDMGVAVYEQAPDAETLLLNDNAPAASSDDEVEEEAEVALSTVDSEFGRTTDPVRM
YMREMGTVELLTREGEIEIAKRIEDGLRHMVMAISACPTTIADILAMAERVANEESRVDELVDGLIDPNAADTDGFSEKD
AEEIENEDAEAEEDDEEEEEDDDGAAQATANAAQLEALKRASLEKFALISEWFDKMRRAFEKEGYKSKSYLKAQETIQNE
LMSIRFTARTVERLCDTLRAQVDEVRQVERQILHIVVDKCGMPRSEFIARFPGSETDLDWAEKVAAEGHSYGAILSRNIP
AIREQQQRLLDLQARVVLPLKDLKETNRQMAAGELKARQAKREMTEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKA
VDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQILQETGLEPDPATLAEKMEMPEDKIR
KIMKIAKEPISMETPIGDDDDSHLGDFIEDTNTVAPADAALHASMRDVVKDVLDSLTPREAKVLRMRFGIEMSTDHTLEE
VGKQFDVTRERIRQIEAKALRKLRHPSRSDKLKSFLEGN

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=619, Percent_Identity=54.6042003231018, Blast_Score=634, Evalue=0.0,
Organism=Escherichia coli, GI1789098, Length=225, Percent_Identity=41.7777777777778, Blast_Score=203, Evalue=4e-53,
Organism=Escherichia coli, GI1789871, Length=243, Percent_Identity=26.7489711934156, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007631
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04546 Sigma70_ner; PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 76746; Mature: 76615

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKLNEVSVDDDANQSDDQPTAASGKGEKAKARDRRAKEKALLKEAFATSTPGTAEELE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
ERRTKLRALIKLGKERGFLTYAEINDHLPDNFTETEALEGIIGTFNDMGVAVYEQAPDAE
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCC
TLLLNDNAPAASSDDEVEEEAEVALSTVDSEFGRTTDPVRMYMREMGTVELLTREGEIEI
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCCCHHH
AKRIEDGLRHMVMAISACPTTIADILAMAERVANEESRVDELVDGLIDPNAADTDGFSEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCC
DAEEIENEDAEAEEDDEEEEEDDDGAAQATANAAQLEALKRASLEKFALISEWFDKMRRA
CHHHHCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FEKEGYKSKSYLKAQETIQNELMSIRFTARTVERLCDTLRAQVDEVRQVERQILHIVVDK
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CGMPRSEFIARFPGSETDLDWAEKVAAEGHSYGAILSRNIPAIREQQQRLLDLQARVVLP
CCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCC
LKDLKETNRQMAAGELKARQAKREMTEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHH
AVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQILQET
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHC
GLEPDPATLAEKMEMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDTNTVAPADA
CCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHH
ALHASMRDVVKDVLDSLTPREAKVLRMRFGIEMSTDHTLEEVGKQFDVTRERIRQIEAKA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LRKLRHPSRSDKLKSFLEGN
HHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure 
TKKLNEVSVDDDANQSDDQPTAASGKGEKAKARDRRAKEKALLKEAFATSTPGTAEELE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
ERRTKLRALIKLGKERGFLTYAEINDHLPDNFTETEALEGIIGTFNDMGVAVYEQAPDAE
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCC
TLLLNDNAPAASSDDEVEEEAEVALSTVDSEFGRTTDPVRMYMREMGTVELLTREGEIEI
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCCCHHH
AKRIEDGLRHMVMAISACPTTIADILAMAERVANEESRVDELVDGLIDPNAADTDGFSEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCC
DAEEIENEDAEAEEDDEEEEEDDDGAAQATANAAQLEALKRASLEKFALISEWFDKMRRA
CHHHHCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FEKEGYKSKSYLKAQETIQNELMSIRFTARTVERLCDTLRAQVDEVRQVERQILHIVVDK
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CGMPRSEFIARFPGSETDLDWAEKVAAEGHSYGAILSRNIPAIREQQQRLLDLQARVVLP
CCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCC
LKDLKETNRQMAAGELKARQAKREMTEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHH
AVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQILQET
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHC
GLEPDPATLAEKMEMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDTNTVAPADA
CCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHH
ALHASMRDVVKDVLDSLTPREAKVLRMRFGIEMSTDHTLEEVGKQFDVTRERIRQIEAKA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LRKLRHPSRSDKLKSFLEGN
HHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA