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The map label for this gene is dnaG

Identifier: 53722777

GI number: 53722777

Start: 2409370

End: 2411244

Strand: Reverse

Name: dnaG

Synonym: BPSS1756

Alternate gene names: 53722777

Gene position: 2411244-2409370 (Counterclockwise)

Preceding gene: 53722778

Following gene: 53722776

Centisome position: 75.99

GC content: 66.72

Gene sequence:

>1875_bases
GTGATTCCGCATTCGTTCCTGCAGGATTTGCTGAACCGCGTCGACATCGTCGACGTGGTCGGCCGGTACGTGCAGCTCAA
GAAGGGCGGCGCCAACTTCATGGGGCTGTGCCCGTTTCACAACGAGAAGAGCCCGTCGTTTACGGTCAGCCCGACGAAGC
AGTTCTATCACTGCTTCGGTTGCGGCGCGCATGGCACCGCGATCGGCTTCCTGATGGAGCACGCGGGGCTCACGTTTCCC
GAGGCGGTGCAGGAGCTCGCGCAATCGGTCGGGCTCACGGTGCCGCACGAGCCGTTGCCGATGCGCGGAGGCGGCGGCTC
GAGCGACGGCCATGCGCCGCCCGCGGCGTCGAAATCCGTCACGACCGCGCTGTCCGACGTGATGCAGACCGCCTGTGACT
ATTACCGCAAGCAGTTGCGCGGCGCGCCGAACGCGATCCAGTACCTGAAGCGGCGCGGCCTGACGGGCGAGATCGCGCTG
CGCTTCGGGCTCGGCTACGCGCCGGACGGCTGGCAGAACCTCGAGGCGGCGTTCGACGACTACCGCCACGATTCGCTCGT
CGACGCGGGCCTCGTGATCGTCAGCGAGAAGACCGACGCGAACGGCGTCGCGCGCCGCTACGACCGGTTCCGCGAGCGGA
TCATGTTCCCGATCCGCAACGTGAAGGGGCAGGTCATCGGCTTCGGCGGCCGGGTGCTGGACGGCGGCGAGCCCAAATAC
CTGAATTCGCCTGAGACGCCGCTGTTCAACAAGGGCAGCGAGCTGTACGGCCTGTTCGAGGCGCGGCTCGCGATTCGCGA
GCACAAGTACGCGCTCGTCGTCGAGGGGTACATGGACGTCGTCGCGCTCGCGCAACTCGGCTTTCCGAACGCGGTGGCGA
CGCTCGGCACCGCGTGCACGCCGATCCACGTGCAGAAGCTGTTGCGGCAGACGGACACGGTCGTCTTCAGTTTCGACGGC
GACGCGGCCGGCCGGCGCGCCGCGCGGCGCGCGCTGGAGGCGTGCCTGCCGCACGCGGCGGACAACCGGACGATCCGTTT
CCTGTTCCTGCCCCCGGAGCACGATCCGGACAGCTATGTGCGGGAATTCGGGACGGATGCGTTTTCGGAACAGGTGAAGC
GGGCGATGCCGCTGTCCCAGTTCCTGCTGAACGAGGCCACGGCAGGTAAGGAGCTTGACCAGCCGGAAGGGCGCGCGAAG
GCGCTGTTCGATGCGAAGCCGCTGCTGCAGGCGCTGCCCGCGAACGCGTTGCGCGCTCAGATCATGCACATGTTCGCCGA
TCGGCTGCAGATTCCGTTCGACGAAGTCGCCGCGCTTGCCGAAGTGGACGCGCGGATCGCCGCGCCGGCCCGCCAGGCGC
CCGCCCGCAACGAGCGGCGGCGCGTGACGGAGAGCGAAAAGCGCGCATTGCGCAATCTGGTGATGCACCCGCGCATCGCG
GCGGCGATCGACGACGAAAGCGTCGCGACGCTGCGGGCGCTGCCCAGGATCGGGGAACTGTTCGACGAGGTGATCGGCCA
CGCGCGCGCGCTTGGGGACGGGGCGGAATTCCGGCTTTTGTCCGACGTGCTACGCAATTCGGCCAACGCGCCGACCTACG
ACGAAATTTTCCGGGAAATTCTGGTCTATGATGAAAATGTCCGCGACTTGATGCAGCAGAATCCGGAAAGCGACGCGGCG
CAGGAAGGGCTGCGCGAGCGCGAACGGATCGCAGGCGAGGAGCTTCAGGCAGCGGTGCTCAAGATGCGCTATGACGCGTG
CTGCGACCGGCTCAACAGCCTGTCGAAGCGCTCGGCGCCCTCTTCCGAGGAATTGGCGGAAATCAAGGACTTGCATCAGA
ACCTGGCAGACATGAAACGGAGGCTCGGGCTGTGA

Upstream 100 bases:

>100_bases
GAAGTAACTTCCCGCCGGCACGGCGCGCGAGCGTCGTTCAGGCGGCCTGCGCCGGCCGCTGCGGCGCGCGTCGCCGGCAC
GTCTTTCGATCGATCCAACG

Downstream 100 bases:

>100_bases
ACCGGCCGGGGAGGGGTCTGCCTCAACGTGCTATAATATAAGGTTTTCAGCGGTTTGTTTTCCTAGCAGAGGCGAGAATC
GCGATGGCAAAGACTACCGG

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 624; Mature: 624

Protein sequence:

>624_residues
MIPHSFLQDLLNRVDIVDVVGRYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFGCGAHGTAIGFLMEHAGLTFP
EAVQELAQSVGLTVPHEPLPMRGGGGSSDGHAPPAASKSVTTALSDVMQTACDYYRKQLRGAPNAIQYLKRRGLTGEIAL
RFGLGYAPDGWQNLEAAFDDYRHDSLVDAGLVIVSEKTDANGVARRYDRFRERIMFPIRNVKGQVIGFGGRVLDGGEPKY
LNSPETPLFNKGSELYGLFEARLAIREHKYALVVEGYMDVVALAQLGFPNAVATLGTACTPIHVQKLLRQTDTVVFSFDG
DAAGRRAARRALEACLPHAADNRTIRFLFLPPEHDPDSYVREFGTDAFSEQVKRAMPLSQFLLNEATAGKELDQPEGRAK
ALFDAKPLLQALPANALRAQIMHMFADRLQIPFDEVAALAEVDARIAAPARQAPARNERRRVTESEKRALRNLVMHPRIA
AAIDDESVATLRALPRIGELFDEVIGHARALGDGAEFRLLSDVLRNSANAPTYDEIFREILVYDENVRDLMQQNPESDAA
QEGLRERERIAGEELQAAVLKMRYDACCDRLNSLSKRSAPSSEELAEIKDLHQNLADMKRRLGL

Sequences:

>Translated_624_residues
MIPHSFLQDLLNRVDIVDVVGRYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFGCGAHGTAIGFLMEHAGLTFP
EAVQELAQSVGLTVPHEPLPMRGGGGSSDGHAPPAASKSVTTALSDVMQTACDYYRKQLRGAPNAIQYLKRRGLTGEIAL
RFGLGYAPDGWQNLEAAFDDYRHDSLVDAGLVIVSEKTDANGVARRYDRFRERIMFPIRNVKGQVIGFGGRVLDGGEPKY
LNSPETPLFNKGSELYGLFEARLAIREHKYALVVEGYMDVVALAQLGFPNAVATLGTACTPIHVQKLLRQTDTVVFSFDG
DAAGRRAARRALEACLPHAADNRTIRFLFLPPEHDPDSYVREFGTDAFSEQVKRAMPLSQFLLNEATAGKELDQPEGRAK
ALFDAKPLLQALPANALRAQIMHMFADRLQIPFDEVAALAEVDARIAAPARQAPARNERRRVTESEKRALRNLVMHPRIA
AAIDDESVATLRALPRIGELFDEVIGHARALGDGAEFRLLSDVLRNSANAPTYDEIFREILVYDENVRDLMQQNPESDAA
QEGLRERERIAGEELQAAVLKMRYDACCDRLNSLSKRSAPSSEELAEIKDLHQNLADMKRRLGL
>Mature_624_residues
MIPHSFLQDLLNRVDIVDVVGRYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFGCGAHGTAIGFLMEHAGLTFP
EAVQELAQSVGLTVPHEPLPMRGGGGSSDGHAPPAASKSVTTALSDVMQTACDYYRKQLRGAPNAIQYLKRRGLTGEIAL
RFGLGYAPDGWQNLEAAFDDYRHDSLVDAGLVIVSEKTDANGVARRYDRFRERIMFPIRNVKGQVIGFGGRVLDGGEPKY
LNSPETPLFNKGSELYGLFEARLAIREHKYALVVEGYMDVVALAQLGFPNAVATLGTACTPIHVQKLLRQTDTVVFSFDG
DAAGRRAARRALEACLPHAADNRTIRFLFLPPEHDPDSYVREFGTDAFSEQVKRAMPLSQFLLNEATAGKELDQPEGRAK
ALFDAKPLLQALPANALRAQIMHMFADRLQIPFDEVAALAEVDARIAAPARQAPARNERRRVTESEKRALRNLVMHPRIA
AAIDDESVATLRALPRIGELFDEVIGHARALGDGAEFRLLSDVLRNSANAPTYDEIFREILVYDENVRDLMQQNPESDAA
QEGLRERERIAGEELQAAVLKMRYDACCDRLNSLSKRSAPSSEELAEIKDLHQNLADMKRRLGL

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=550, Percent_Identity=42.3636363636364, Blast_Score=399, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 68962; Mature: 68962

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPHSFLQDLLNRVDIVDVVGRYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEECCHHHHHHHHC
CGAHGTAIGFLMEHAGLTFPEAVQELAQSVGLTVPHEPLPMRGGGGSSDGHAPPAASKSV
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
TTALSDVMQTACDYYRKQLRGAPNAIQYLKRRGLTGEIALRFGLGYAPDGWQNLEAAFDD
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEHEEECCCCCCCCHHHHHHHHHH
YRHDSLVDAGLVIVSEKTDANGVARRYDRFRERIMFPIRNVKGQVIGFGGRVLDGGEPKY
HHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEECCCCCCC
LNSPETPLFNKGSELYGLFEARLAIREHKYALVVEGYMDVVALAQLGFPNAVATLGTACT
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHCCCCHHHHHHHCCCC
PIHVQKLLRQTDTVVFSFDGDAAGRRAARRALEACLPHAADNRTIRFLFLPPEHDPDSYV
HHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHH
REFGTDAFSEQVKRAMPLSQFLLNEATAGKELDQPEGRAKALFDAKPLLQALPANALRAQ
HHHCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHH
IMHMFADRLQIPFDEVAALAEVDARIAAPARQAPARNERRRVTESEKRALRNLVMHPRIA
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCHH
AAIDDESVATLRALPRIGELFDEVIGHARALGDGAEFRLLSDVLRNSANAPTYDEIFREI
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHH
LVYDENVRDLMQQNPESDAAQEGLRERERIAGEELQAAVLKMRYDACCDRLNSLSKRSAP
HHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SSEELAEIKDLHQNLADMKRRLGL
CHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIPHSFLQDLLNRVDIVDVVGRYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEECCHHHHHHHHC
CGAHGTAIGFLMEHAGLTFPEAVQELAQSVGLTVPHEPLPMRGGGGSSDGHAPPAASKSV
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
TTALSDVMQTACDYYRKQLRGAPNAIQYLKRRGLTGEIALRFGLGYAPDGWQNLEAAFDD
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEHEEECCCCCCCCHHHHHHHHHH
YRHDSLVDAGLVIVSEKTDANGVARRYDRFRERIMFPIRNVKGQVIGFGGRVLDGGEPKY
HHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEECCCCCCC
LNSPETPLFNKGSELYGLFEARLAIREHKYALVVEGYMDVVALAQLGFPNAVATLGTACT
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHCCCCHHHHHHHCCCC
PIHVQKLLRQTDTVVFSFDGDAAGRRAARRALEACLPHAADNRTIRFLFLPPEHDPDSYV
HHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHH
REFGTDAFSEQVKRAMPLSQFLLNEATAGKELDQPEGRAKALFDAKPLLQALPANALRAQ
HHHCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHH
IMHMFADRLQIPFDEVAALAEVDARIAAPARQAPARNERRRVTESEKRALRNLVMHPRIA
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCHH
AAIDDESVATLRALPRIGELFDEVIGHARALGDGAEFRLLSDVLRNSANAPTYDEIFREI
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHH
LVYDENVRDLMQQNPESDAAQEGLRERERIAGEELQAAVLKMRYDACCDRLNSLSKRSAP
HHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SSEELAEIKDLHQNLADMKRRLGL
CHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]