Click here to switch to the map view.

The map label for this gene is hycE [H]

Identifier: 53722171

GI number: 53722171

Start: 1529032

End: 1530687

Strand: Reverse

Name: hycE [H]

Synonym: BPSS1143

Alternate gene names: 53722171

Gene position: 1530687-1529032 (Counterclockwise)

Preceding gene: 53722172

Following gene: 53722170

Centisome position: 48.24

GC content: 72.83

Gene sequence:

>1656_bases
GTGTTCGCGTCGGTCGACGCCGACGCATGGACGCGCGCCGCCGAGACGATCCGGACGGCCGGCGGGCGGCTCGTCACGCT
GTGGGGCGGCGAGCCCGCGGCCGGGCGCTACGAGGTCTGCGCCGCGTACGAGCTGGACGACGGACTGTTGTGGCTGCGCC
TGCCGGTCGAGCCGGCGGCGAACGTGGGGCGGGAAGCCGGCGACACGAGCAACGCGGGCAACGCGAGCAATGCGGGCGAC
GCGGACGGCGTGCGGCAACGCGGCGAGGCCGGCAGCTATCCGGATCTGTCGGCGATCTTCGCGAGCGCGACGCGGATGCA
ACGGGCGCTTCACGATCTCGTCGGCCTGCGCGCGCGCGGCGCGCACGACACGCGGCCGTGGCTCGCCCACGGCGCATGGC
CGCGCGATTACCACCCGCTGCGCAGGCGCGCGACGGGCGGCGAGCGCTTCGAGTACGCGACGGCCGACTATCCGTTCGTG
CCGGTCGCGGGCGACGGCGTGCATGAGATCGCGGTCGGGCCGATTCATGCGGGCGTGATCGAGCCCGGGCATTTCCGCTT
TTCCGTCGTCGGCGAAAAGGTGCTGCGGCTCGAGGAGCGGCTTGGCTACGCGCATCGCGGCGTCGAACGGCTCTTCGAGC
GCGCGGATGCGCTCGCGGGCAGCCGGCTCGCCGCGCGCATCGCCGGCGATTCGACGGTCGCGTTCGCGTGGGCGTACTGC
ATGGCGCTCGAACAGGCGTTGAGAATCCGCGTGCCGGACCGCGCGCTGCGGCTGCGCGCGCTGCTGCTCGAGCGCGAGCG
CGTCGCGAACCATCTGGGCGACCTCGGCGCGCTCGGCAACGACGCGGGTTTTGCCGTGGGCCTCGCGCATTTCTCGCGGC
TCAAGGAAGACTGGCTGCGGATGCAGGACCGGATCTTCGGGCATCGCTACCTGATGGACGCGATCGTGCCGGGCGGCGTC
GCGCGCGACATCGCGGCGGGCGACGCCGCGGCGATCGTCGCGCAGTGCGACCGGATCGAGCGTGAAGTGCGCGCGATGCA
AACGATCTACGACGATCAATCCGGCCTGCAGGACCGCTTCGCCGGCACCGGGCGGCTCGACGCGCAGGCGGTCGCGCATT
TCGGCGTATGCGGGCTCGCCGCCCGGGCGAGCGGGCAGCCGCGCGACGTGCGCGCGGACCATCGGCTGGCGCCATACGAC
GCGCTGCGCATCGACGTCAGCCGCGACGCGCGCGGCGACGTCGCGGCGCGCGTCGCGGTGCGCTTCGCCGAAACGTACGA
GTCGATGCGCGCGATCCGCGCGCTGCTCGCGGATTTGCCGGACGACGGGCTCGCCGCGGAGATCGCGCCCGGCGCGACTG
TTCCGGGCGCGCGCGCCGATGCGGGCGGAGCATGCGAAGGGGAAGAGGGGGGCGACGCCGCATTCTGCCCGGGCGACGCG
CGGCGGCGCGGCATCGGCTGGATCGAAGGCTGGCGCGGGCCCGTGTTCGTCGCGCTCGAACTCGATGCGCGCGGCGCCGT
CGCGCGCTGCCATTGCCACGATCCGTCATGGCACAACTGGCCCGCGCTCGAGCATGCGATCATCGGCAACATCGTCGCGG
ACTTCCCGTTGATCAACAAGTCGTTCAACCTGAACTACGCGGGGCACGACCTGTAA

Upstream 100 bases:

>100_bases
GCAGGCGGCGGCGATGATCGCGGGGTAGCCGATGCGAATCGACGCATTGCGAATGTCCGGTCAGACGCGCATCGCGAGCG
CGGCGGGGCAGCCGCGCGCC

Downstream 100 bases:

>100_bases
TGTGGCAACTCATCAAACAGATCGCGCGCACCGGCCGGCCTAACGAACCCGCGCCGGCGGGCGAAGACGCCTGGCGGCGG
GATGCGCAGTGCATTCGGCA

Product: formate hydrogenlyase/hydrogenase/NADH dehydrogenase subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 5; Hydrogenase-3 component E [H]

Number of amino acids: Translated: 551; Mature: 551

Protein sequence:

>551_residues
MFASVDADAWTRAAETIRTAGGRLVTLWGGEPAAGRYEVCAAYELDDGLLWLRLPVEPAANVGREAGDTSNAGNASNAGD
ADGVRQRGEAGSYPDLSAIFASATRMQRALHDLVGLRARGAHDTRPWLAHGAWPRDYHPLRRRATGGERFEYATADYPFV
PVAGDGVHEIAVGPIHAGVIEPGHFRFSVVGEKVLRLEERLGYAHRGVERLFERADALAGSRLAARIAGDSTVAFAWAYC
MALEQALRIRVPDRALRLRALLLERERVANHLGDLGALGNDAGFAVGLAHFSRLKEDWLRMQDRIFGHRYLMDAIVPGGV
ARDIAAGDAAAIVAQCDRIEREVRAMQTIYDDQSGLQDRFAGTGRLDAQAVAHFGVCGLAARASGQPRDVRADHRLAPYD
ALRIDVSRDARGDVAARVAVRFAETYESMRAIRALLADLPDDGLAAEIAPGATVPGARADAGGACEGEEGGDAAFCPGDA
RRRGIGWIEGWRGPVFVALELDARGAVARCHCHDPSWHNWPALEHAIIGNIVADFPLINKSFNLNYAGHDL

Sequences:

>Translated_551_residues
MFASVDADAWTRAAETIRTAGGRLVTLWGGEPAAGRYEVCAAYELDDGLLWLRLPVEPAANVGREAGDTSNAGNASNAGD
ADGVRQRGEAGSYPDLSAIFASATRMQRALHDLVGLRARGAHDTRPWLAHGAWPRDYHPLRRRATGGERFEYATADYPFV
PVAGDGVHEIAVGPIHAGVIEPGHFRFSVVGEKVLRLEERLGYAHRGVERLFERADALAGSRLAARIAGDSTVAFAWAYC
MALEQALRIRVPDRALRLRALLLERERVANHLGDLGALGNDAGFAVGLAHFSRLKEDWLRMQDRIFGHRYLMDAIVPGGV
ARDIAAGDAAAIVAQCDRIEREVRAMQTIYDDQSGLQDRFAGTGRLDAQAVAHFGVCGLAARASGQPRDVRADHRLAPYD
ALRIDVSRDARGDVAARVAVRFAETYESMRAIRALLADLPDDGLAAEIAPGATVPGARADAGGACEGEEGGDAAFCPGDA
RRRGIGWIEGWRGPVFVALELDARGAVARCHCHDPSWHNWPALEHAIIGNIVADFPLINKSFNLNYAGHDL
>Mature_551_residues
MFASVDADAWTRAAETIRTAGGRLVTLWGGEPAAGRYEVCAAYELDDGLLWLRLPVEPAANVGREAGDTSNAGNASNAGD
ADGVRQRGEAGSYPDLSAIFASATRMQRALHDLVGLRARGAHDTRPWLAHGAWPRDYHPLRRRATGGERFEYATADYPFV
PVAGDGVHEIAVGPIHAGVIEPGHFRFSVVGEKVLRLEERLGYAHRGVERLFERADALAGSRLAARIAGDSTVAFAWAYC
MALEQALRIRVPDRALRLRALLLERERVANHLGDLGALGNDAGFAVGLAHFSRLKEDWLRMQDRIFGHRYLMDAIVPGGV
ARDIAAGDAAAIVAQCDRIEREVRAMQTIYDDQSGLQDRFAGTGRLDAQAVAHFGVCGLAARASGQPRDVRADHRLAPYD
ALRIDVSRDARGDVAARVAVRFAETYESMRAIRALLADLPDDGLAAEIAPGATVPGARADAGGACEGEEGGDAAFCPGDA
RRRGIGWIEGWRGPVFVALELDARGAVARCHCHDPSWHNWPALEHAIIGNIVADFPLINKSFNLNYAGHDL

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=367, Percent_Identity=24.7956403269755, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI260898743, Length=367, Percent_Identity=24.7956403269755, Blast_Score=102, Evalue=1e-21,
Organism=Escherichia coli, GI1789076, Length=465, Percent_Identity=31.1827956989247, Blast_Score=203, Evalue=3e-53,
Organism=Escherichia coli, GI1788832, Length=466, Percent_Identity=29.6137339055794, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI145693162, Length=466, Percent_Identity=22.3175965665236, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17555284, Length=371, Percent_Identity=23.7196765498652, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17568379, Length=373, Percent_Identity=24.1286863270777, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI221459469, Length=271, Percent_Identity=27.6752767527675, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24638644, Length=362, Percent_Identity=24.8618784530387, Blast_Score=104, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001268
- InterPro:   IPR014029
- InterPro:   IPR001501
- ProDom:   PD001581 [H]

Pfam domain/function: PF00329 Complex1_30kDa; PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 59414; Mature: 59414

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFASVDADAWTRAAETIRTAGGRLVTLWGGEPAAGRYEVCAAYELDDGLLWLRLPVEPAA
CCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEEEECCCEEEEEECCCHHH
NVGREAGDTSNAGNASNAGDADGVRQRGEAGSYPDLSAIFASATRMQRALHDLVGLRARG
HCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
AHDTRPWLAHGAWPRDYHPLRRRATGGERFEYATADYPFVPVAGDGVHEIAVGPIHAGVI
CCCCCCHHHCCCCCCCCCHHHHHCCCCCCEEEECCCCCEEEECCCCCCEEEECCEECCCC
EPGHFRFSVVGEKVLRLEERLGYAHRGVERLFERADALAGSRLAARIAGDSTVAFAWAYC
CCCCEEEHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHEEECCCCHHHHHHHHH
MALEQALRIRVPDRALRLRALLLERERVANHLGDLGALGNDAGFAVGLAHFSRLKEDWLR
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
MQDRIFGHRYLMDAIVPGGVARDIAAGDAAAIVAQCDRIEREVRAMQTIYDDQSGLQDRF
HHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
AGTGRLDAQAVAHFGVCGLAARASGQPRDVRADHRLAPYDALRIDVSRDARGDVAARVAV
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHH
RFAETYESMRAIRALLADLPDDGLAAEIAPGATVPGARADAGGACEGEEGGDAAFCPGDA
HHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCEEECCCHH
RRRGIGWIEGWRGPVFVALELDARGAVARCHCHDPSWHNWPALEHAIIGNIVADFPLINK
HHCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCC
SFNLNYAGHDL
CCCCCCCCCCC
>Mature Secondary Structure
MFASVDADAWTRAAETIRTAGGRLVTLWGGEPAAGRYEVCAAYELDDGLLWLRLPVEPAA
CCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEEEECCCEEEEEECCCHHH
NVGREAGDTSNAGNASNAGDADGVRQRGEAGSYPDLSAIFASATRMQRALHDLVGLRARG
HCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
AHDTRPWLAHGAWPRDYHPLRRRATGGERFEYATADYPFVPVAGDGVHEIAVGPIHAGVI
CCCCCCHHHCCCCCCCCCHHHHHCCCCCCEEEECCCCCEEEECCCCCCEEEECCEECCCC
EPGHFRFSVVGEKVLRLEERLGYAHRGVERLFERADALAGSRLAARIAGDSTVAFAWAYC
CCCCEEEHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHEEECCCCHHHHHHHHH
MALEQALRIRVPDRALRLRALLLERERVANHLGDLGALGNDAGFAVGLAHFSRLKEDWLR
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
MQDRIFGHRYLMDAIVPGGVARDIAAGDAAAIVAQCDRIEREVRAMQTIYDDQSGLQDRF
HHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
AGTGRLDAQAVAHFGVCGLAARASGQPRDVRADHRLAPYDALRIDVSRDARGDVAARVAV
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHH
RFAETYESMRAIRALLADLPDDGLAAEIAPGATVPGARADAGGACEGEEGGDAAFCPGDA
HHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCEEECCCHH
RRRGIGWIEGWRGPVFVALELDARGAVARCHCHDPSWHNWPALEHAIIGNIVADFPLINK
HHCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCC
SFNLNYAGHDL
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503; 8125094 [H]