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The map label for this gene is hycG [H]

Identifier: 53722170

GI number: 53722170

Start: 1528514

End: 1529032

Strand: Reverse

Name: hycG [H]

Synonym: BPSS1142

Alternate gene names: 53722170

Gene position: 1529032-1528514 (Counterclockwise)

Preceding gene: 53722171

Following gene: 53722169

Centisome position: 48.19

GC content: 71.1

Gene sequence:

>519_bases
ATGTGGCAACTCATCAAACAGATCGCGCGCACCGGCCGGCCTAACGAACCCGCGCCGGCGGGCGAAGACGCCTGGCGGCG
GGATGCGCAGTGCATTCGGCAGGAGATACTCGACATCCTCGGCCGCGCGCTGTGCATCCGCGCGATCGACGCCGGCTCGT
GCAACGGCTGCGAGCTCGAACTGCACGCGCTCGGCAATCCGTACTACAACATCGAGGGCCTCGGCATCAAGTTCGTCGCG
AGCCCGCGGCACGCGGACATGCTGCTGATCACCGGGCCGCTCACGCTGAACATGCGCGACGCCGTGCGGCTCGCGTACGA
CGCGACGCCCGCGCCGAAGCTCGTCGTCGCGGCGGGCGATTGCGCATGCACGGGCGGCGTGTTCGCGGGCAGCTACGCGG
TGTGCGGGCCCATTGAGACGCTCGTGCCGGTCGACGTCGCCATTCCGGGCTGCCCGCCGCGCCCGATCGAGCTGCTGCGC
GGCATTCGCGGCGCGCTCGAGCGCACGGCCGCGCGCTGA

Upstream 100 bases:

>100_bases
ACTGGCCCGCGCTCGAGCATGCGATCATCGGCAACATCGTCGCGGACTTCCCGTTGATCAACAAGTCGTTCAACCTGAAC
TACGCGGGGCACGACCTGTA

Downstream 100 bases:

>100_bases
GGCGCGGGCGCGACCGGATGCGCCACGCGGCGCTGCGCGGCGCGCGACGATTTCTTCCGGAATGGCGCGGTTGGCTGCGT
GACGGTTGCGCGCCGGATTT

Product: hydrogenase/oxidoreductase subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 7; Hydrogenase-3 component G [H]

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MWQLIKQIARTGRPNEPAPAGEDAWRRDAQCIRQEILDILGRALCIRAIDAGSCNGCELELHALGNPYYNIEGLGIKFVA
SPRHADMLLITGPLTLNMRDAVRLAYDATPAPKLVVAAGDCACTGGVFAGSYAVCGPIETLVPVDVAIPGCPPRPIELLR
GIRGALERTAAR

Sequences:

>Translated_172_residues
MWQLIKQIARTGRPNEPAPAGEDAWRRDAQCIRQEILDILGRALCIRAIDAGSCNGCELELHALGNPYYNIEGLGIKFVA
SPRHADMLLITGPLTLNMRDAVRLAYDATPAPKLVVAAGDCACTGGVFAGSYAVCGPIETLVPVDVAIPGCPPRPIELLR
GIRGALERTAAR
>Mature_172_residues
MWQLIKQIARTGRPNEPAPAGEDAWRRDAQCIRQEILDILGRALCIRAIDAGSCNGCELELHALGNPYYNIEGLGIKFVA
SPRHADMLLITGPLTLNMRDAVRLAYDATPAPKLVVAAGDCACTGGVFAGSYAVCGPIETLVPVDVAIPGCPPRPIELLR
GIRGALERTAAR

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=106, Percent_Identity=42.4528301886792, Blast_Score=96, Evalue=2e-20,
Organism=Escherichia coli, GI1789074, Length=135, Percent_Identity=42.962962962963, Blast_Score=118, Evalue=2e-28,
Organism=Escherichia coli, GI1788834, Length=138, Percent_Identity=42.0289855072464, Blast_Score=117, Evalue=3e-28,
Organism=Escherichia coli, GI1788624, Length=124, Percent_Identity=33.0645161290323, Blast_Score=72, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17509685, Length=158, Percent_Identity=33.5443037974684, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24651058, Length=157, Percent_Identity=35.6687898089172, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI18859983, Length=106, Percent_Identity=38.6792452830189, Blast_Score=93, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24642371, Length=106, Percent_Identity=38.6792452830189, Blast_Score=93, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: NA

Molecular weight: Translated: 18357; Mature: 18357

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
4.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWQLIKQIARTGRPNEPAPAGEDAWRRDAQCIRQEILDILGRALCIRAIDAGSCNGCELE
CHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
LHALGNPYYNIEGLGIKFVASPRHADMLLITGPLTLNMRDAVRLAYDATPAPKLVVAAGD
EEECCCCEEEEECCCEEEEECCCCCCEEEEECCEEECHHHHEEEEECCCCCCEEEEEECC
CACTGGVFAGSYAVCGPIETLVPVDVAIPGCPPRPIELLRGIRGALERTAAR
CCCCCCCCCCCEEECCCHHHHCEEEEECCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MWQLIKQIARTGRPNEPAPAGEDAWRRDAQCIRQEILDILGRALCIRAIDAGSCNGCELE
CHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
LHALGNPYYNIEGLGIKFVASPRHADMLLITGPLTLNMRDAVRLAYDATPAPKLVVAAGD
EEECCCCEEEEECCCEEEEECCCCCCEEEEECCEEECHHHHEEEEECCCCCCEEEEEECC
CACTGGVFAGSYAVCGPIETLVPVDVAIPGCPPRPIELLRGIRGALERTAAR
CCCCCCCCCCCEEECCCHHHHCEEEEECCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]