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The map label for this gene is hyfF [H]

Identifier: 53722172

GI number: 53722172

Start: 1530760

End: 1532220

Strand: Reverse

Name: hyfF [H]

Synonym: BPSS1144

Alternate gene names: 53722172

Gene position: 1532220-1530760 (Counterclockwise)

Preceding gene: 53722173

Following gene: 53722171

Centisome position: 48.29

GC content: 67.97

Gene sequence:

>1461_bases
ATGATTGACGGCTACGTGCTGCCCGCGTTGCTCGGCCTGCCGCTCGCGGCGGGCGGGTGCCTCGCGCTCGTGCGGCGCCA
GCACGTCGCGCGCGTGCTGAACGTCGGCTTCAGCTTTCTCACGTGCGCGGCGGCGCTCGCGCTCGCCGCGCGCACCGTCG
CGCACGGGCCGATGTACGCGGTGGGCGCGCTCTTCTTCGTCGATCCGTTCAACGTGTTCCTCGTCGCGCTGACGGCGTTC
GTCGGCTGGACGACATCGCTGTTCTCGAATCCGTACATGCGCATCGAGCACGATCGCGGCAAGATGAGCGACGGCCGCAT
GCGGCTCTATCACTGCATGTACCAGTTGTTCATTTTCGCGATGCTGCTCGCGCTGCTCACGAACAACGTCGGCGTGCTGT
GGGTCGCGATGGAGGCGGCGACGCTCGCGACGGTGCTGCTCGTGAGCGTGTACCGCACCGCGGCGAGCCTCGAGGCCGCA
TGGAAATACTTCATCCTGTGCGGCGTCGGCATCGCGCAGGCGCTGTTCGGCACGATCCTGCTGTATCTCGCGGCCGGGCG
GCGGCTCGCGGACGGCGACGCGCTGCTCTGGACGAGCCTGAACGCCGTGAAGACGCAGCTCGATCCGACGATCGTGTCGA
TCGCGTTCGTGTTCCTGCTCGTCGGCTACGGCACCAAGGTCGGGCTCGTGCCGATGCACAGCTGGCTGCCCGATGCGCAC
GCCGAAGGCCCGACGCCGATCTCGGCGGTGCTCTCGGGGCTGCTGCTGAACGTCGCGCTGTACGCGGTGCTGCGCTGCAA
GGTGCTCGCCGACGGCGCGCTCGGCAACGGGCTGCCCGGACATCTGCTGGTGGGCTTCGGGCTCGTGTCGGTGCTCGTCG
CGTCGTTCTCGCTGTTCCGGCAAAAGGACGTGAAGCGGTTGTTCTCGTACTCGTCGATCGAGCACATGGGCCTGATGACG
TTCGCATTCGGCCTGGGCGGCCCGATCGCGACGTTCGCGGGGCTCTTGCACATGACGGTTCACTCGCTCGTCAAATCGGC
GATCTTCTTCGCGGTCGGGCATGCGGCGCAGAAGGCGCGCACGCAGGCGATCGACGGCATCCGCGGGCTGCTGCAGGTGA
GCCCGACGGTCGGCTGGGGGATGATGCTCGGCACGCTCGCGATCCTCGGCATGCCGCCGTTCGGCGTGTTCGCGAGCGAA
TTCCTGATCCTGACGACGGCGATCGCGAAGCTGCCATGGAGCGCGCCGTTCCTGCTCGTCGCGCTCGCGGCGGCGTTCGC
GGCGATCTTCATGCGCGTGCAGCCGATGGTGTTCGGCGAATCGAGCGTCGCCTCGCTCGAGCATCCGCCCGCGCTGCTGC
CGGTGTTCGTTCACCTCGCACTCGGCTTGATGCTCGGGCTTTACGTGCCGCCGTATCTGGCCGCCTGGTATCGGCAGGCG
GCGGCGATGATCGCGGGGTAG

Upstream 100 bases:

>100_bases
CTCGACGTGCTGGTCGGCATCCTGATCCTCGGCGTGTTCATGTTCCAGATCCGCGAGCAGTTCGACAGCCTCGACATCCA
CCACCTCGAGAAACTGAAAG

Downstream 100 bases:

>100_bases
CCGATGCGAATCGACGCATTGCGAATGTCCGGTCAGACGCGCATCGCGAGCGCGGCGGGGCAGCCGCGCGCCGTGTTCGC
GTCGGTCGACGCCGACGCAT

Product: hydrogenase 4 subunit F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MIDGYVLPALLGLPLAAGGCLALVRRQHVARVLNVGFSFLTCAAALALAARTVAHGPMYAVGALFFVDPFNVFLVALTAF
VGWTTSLFSNPYMRIEHDRGKMSDGRMRLYHCMYQLFIFAMLLALLTNNVGVLWVAMEAATLATVLLVSVYRTAASLEAA
WKYFILCGVGIAQALFGTILLYLAAGRRLADGDALLWTSLNAVKTQLDPTIVSIAFVFLLVGYGTKVGLVPMHSWLPDAH
AEGPTPISAVLSGLLLNVALYAVLRCKVLADGALGNGLPGHLLVGFGLVSVLVASFSLFRQKDVKRLFSYSSIEHMGLMT
FAFGLGGPIATFAGLLHMTVHSLVKSAIFFAVGHAAQKARTQAIDGIRGLLQVSPTVGWGMMLGTLAILGMPPFGVFASE
FLILTTAIAKLPWSAPFLLVALAAAFAAIFMRVQPMVFGESSVASLEHPPALLPVFVHLALGLMLGLYVPPYLAAWYRQA
AAMIAG

Sequences:

>Translated_486_residues
MIDGYVLPALLGLPLAAGGCLALVRRQHVARVLNVGFSFLTCAAALALAARTVAHGPMYAVGALFFVDPFNVFLVALTAF
VGWTTSLFSNPYMRIEHDRGKMSDGRMRLYHCMYQLFIFAMLLALLTNNVGVLWVAMEAATLATVLLVSVYRTAASLEAA
WKYFILCGVGIAQALFGTILLYLAAGRRLADGDALLWTSLNAVKTQLDPTIVSIAFVFLLVGYGTKVGLVPMHSWLPDAH
AEGPTPISAVLSGLLLNVALYAVLRCKVLADGALGNGLPGHLLVGFGLVSVLVASFSLFRQKDVKRLFSYSSIEHMGLMT
FAFGLGGPIATFAGLLHMTVHSLVKSAIFFAVGHAAQKARTQAIDGIRGLLQVSPTVGWGMMLGTLAILGMPPFGVFASE
FLILTTAIAKLPWSAPFLLVALAAAFAAIFMRVQPMVFGESSVASLEHPPALLPVFVHLALGLMLGLYVPPYLAAWYRQA
AAMIAG
>Mature_486_residues
MIDGYVLPALLGLPLAAGGCLALVRRQHVARVLNVGFSFLTCAAALALAARTVAHGPMYAVGALFFVDPFNVFLVALTAF
VGWTTSLFSNPYMRIEHDRGKMSDGRMRLYHCMYQLFIFAMLLALLTNNVGVLWVAMEAATLATVLLVSVYRTAASLEAA
WKYFILCGVGIAQALFGTILLYLAAGRRLADGDALLWTSLNAVKTQLDPTIVSIAFVFLLVGYGTKVGLVPMHSWLPDAH
AEGPTPISAVLSGLLLNVALYAVLRCKVLADGALGNGLPGHLLVGFGLVSVLVASFSLFRQKDVKRLFSYSSIEHMGLMT
FAFGLGGPIATFAGLLHMTVHSLVKSAIFFAVGHAAQKARTQAIDGIRGLLQVSPTVGWGMMLGTLAILGMPPFGVFASE
FLILTTAIAKLPWSAPFLLVALAAAFAAIFMRVQPMVFGESSVASLEHPPALLPVFVHLALGLMLGLYVPPYLAAWYRQA
AAMIAG

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=345, Percent_Identity=26.0869565217391, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI251831116, Length=235, Percent_Identity=27.6595744680851, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1788831, Length=390, Percent_Identity=41.5384615384615, Blast_Score=297, Evalue=1e-81,
Organism=Escherichia coli, GI1788827, Length=349, Percent_Identity=27.7936962750716, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1788613, Length=238, Percent_Identity=28.9915966386555, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788829, Length=343, Percent_Identity=27.6967930029155, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788614, Length=350, Percent_Identity=26.2857142857143, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI145693160, Length=412, Percent_Identity=24.7572815533981, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI2367154, Length=388, Percent_Identity=22.9381443298969, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 51789; Mature: 51789

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDGYVLPALLGLPLAAGGCLALVRRQHVARVLNVGFSFLTCAAALALAARTVAHGPMYA
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
VGALFFVDPFNVFLVALTAFVGWTTSLFSNPYMRIEHDRGKMSDGRMRLYHCMYQLFIFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHH
MLLALLTNNVGVLWVAMEAATLATVLLVSVYRTAASLEAAWKYFILCGVGIAQALFGTIL
HHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYLAAGRRLADGDALLWTSLNAVKTQLDPTIVSIAFVFLLVGYGTKVGLVPMHSWLPDAH
HHHHCCCCCCCCCEEHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEECHHCCCCCC
AEGPTPISAVLSGLLLNVALYAVLRCKVLADGALGNGLPGHLLVGFGLVSVLVASFSLFR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QKDVKRLFSYSSIEHMGLMTFAFGLGGPIATFAGLLHMTVHSLVKSAIFFAVGHAAQKAR
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TQAIDGIRGLLQVSPTVGWGMMLGTLAILGMPPFGVFASEFLILTTAIAKLPWSAPFLLV
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHH
ALAAAFAAIFMRVQPMVFGESSVASLEHPPALLPVFVHLALGLMLGLYVPPYLAAWYRQA
HHHHHHHHHHHHHHHHHCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
AAMIAG
HHHHCC
>Mature Secondary Structure
MIDGYVLPALLGLPLAAGGCLALVRRQHVARVLNVGFSFLTCAAALALAARTVAHGPMYA
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
VGALFFVDPFNVFLVALTAFVGWTTSLFSNPYMRIEHDRGKMSDGRMRLYHCMYQLFIFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHH
MLLALLTNNVGVLWVAMEAATLATVLLVSVYRTAASLEAAWKYFILCGVGIAQALFGTIL
HHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYLAAGRRLADGDALLWTSLNAVKTQLDPTIVSIAFVFLLVGYGTKVGLVPMHSWLPDAH
HHHHCCCCCCCCCEEHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEECHHCCCCCC
AEGPTPISAVLSGLLLNVALYAVLRCKVLADGALGNGLPGHLLVGFGLVSVLVASFSLFR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QKDVKRLFSYSSIEHMGLMTFAFGLGGPIATFAGLLHMTVHSLVKSAIFFAVGHAAQKAR
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TQAIDGIRGLLQVSPTVGWGMMLGTLAILGMPPFGVFASEFLILTTAIAKLPWSAPFLLV
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHH
ALAAAFAAIFMRVQPMVFGESSVASLEHPPALLPVFVHLALGLMLGLYVPPYLAAWYRQA
HHHHHHHHHHHHHHHHHCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
AAMIAG
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA