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The map label for this gene is puuD [H]

Identifier: 53721507

GI number: 53721507

Start: 640539

End: 641339

Strand: Reverse

Name: puuD [H]

Synonym: BPSS0470

Alternate gene names: 53721507

Gene position: 641339-640539 (Counterclockwise)

Preceding gene: 53721515

Following gene: 53721506

Centisome position: 20.21

GC content: 71.16

Gene sequence:

>801_bases
ATGTCAAGCAAACCATTGGTCGGTATCACCGCGGATAAAAAGATGATGGGCGCGCATCCATCGCATGTGGTCGTCGAGAA
GTACGTCACGGCCGTCGTCGACGGCGCCGATGCGCTCGCGATGCTCGTGCCCGCGCTCGGCGCGCGCCAGGCGCCGGGCG
ACGTGCTCGCCGCGATCGACGGGCTGCTGTTGACGGGCAGCTATTCGAACGTCGAACCGCATCGCTACGGCGGCGAGCCG
AGCGCGCCCGGCACGCTGCACGATCCCGCGCGCGACGAGACGGCGCTGGCGCTCGCGCGCGCGGCGATCGACGCCGGCGT
GCCGGTGCTCGCGATCTGCCGCGGCCTCCAGGAAATGAACGTCGTGTTCGGCGGCACGCTGCACCAGCAGGTGCATGCGG
CGGGCGGATTCGACGATCACCGCGAGGACAAGCGCGACACGCTCGATGCGCAGTACGCGCCGGCACACGAGATCCGCCTC
GCCGAGGGCGGGCTGTTGCGGCGCCTGCTCGGCGGCGCGGCGCATGCGCGCGTGAACTCGCTGCACGGCCAGGGCATCGC
GCAATTGGGCGCGGGACTCGCGATCGAGGCGCTCGCGCCCGACGGCCTGATCGAAGCGGTTCGCGTGCCGCACGCGCGCG
CGTTCGCGCTCGGCGTGCAATGGCATCCGGAGTGGCGGCACGCGAACGATGCGCTTTCCACTGCGATCTTCCGCGCATTC
GGCGACGCGTGCCGCGAACGGATGCGCATCCGCATGCGGCACGACGCCGGCCGGCTGCCCACGCACGCGATCGGCGCGTA
A

Upstream 100 bases:

>100_bases
TGACGCTCCGCGTGAAGCCGACGGGCCCGCTCATGCCTGCCGTATTGCGATTTTCGATGCGCGATTCGCAATCCGGACAT
TCATCGAAACGTGAAATTTC

Downstream 100 bases:

>100_bases
ACCGTCCCACTACAACGAGAACGATCATGCAAGACATCGACGAATTCCTGAAGAAACACCGGATCACCGAGATCGAGGCG
ATCATCCCCGACATGGCCGG

Product: glutamine amidotransferase

Products: NA

Alternate protein names: Gamma-Glu-GABA hydrolase [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MSSKPLVGITADKKMMGAHPSHVVVEKYVTAVVDGADALAMLVPALGARQAPGDVLAAIDGLLLTGSYSNVEPHRYGGEP
SAPGTLHDPARDETALALARAAIDAGVPVLAICRGLQEMNVVFGGTLHQQVHAAGGFDDHREDKRDTLDAQYAPAHEIRL
AEGGLLRRLLGGAAHARVNSLHGQGIAQLGAGLAIEALAPDGLIEAVRVPHARAFALGVQWHPEWRHANDALSTAIFRAF
GDACRERMRIRMRHDAGRLPTHAIGA

Sequences:

>Translated_266_residues
MSSKPLVGITADKKMMGAHPSHVVVEKYVTAVVDGADALAMLVPALGARQAPGDVLAAIDGLLLTGSYSNVEPHRYGGEP
SAPGTLHDPARDETALALARAAIDAGVPVLAICRGLQEMNVVFGGTLHQQVHAAGGFDDHREDKRDTLDAQYAPAHEIRL
AEGGLLRRLLGGAAHARVNSLHGQGIAQLGAGLAIEALAPDGLIEAVRVPHARAFALGVQWHPEWRHANDALSTAIFRAF
GDACRERMRIRMRHDAGRLPTHAIGA
>Mature_265_residues
SSKPLVGITADKKMMGAHPSHVVVEKYVTAVVDGADALAMLVPALGARQAPGDVLAAIDGLLLTGSYSNVEPHRYGGEPS
APGTLHDPARDETALALARAAIDAGVPVLAICRGLQEMNVVFGGTLHQQVHAAGGFDDHREDKRDTLDAQYAPAHEIRLA
EGGLLRRLLGGAAHARVNSLHGQGIAQLGAGLAIEALAPDGLIEAVRVPHARAFALGVQWHPEWRHANDALSTAIFRAFG
DACRERMRIRMRHDAGRLPTHAIGA

Specific function: Involved in the breakdown of putrescine via hydrolysis of the gamma-glutamyl linkage of gamma-glutamyl-gamma- aminobutyrate [H]

COG id: COG2071

COG function: function code R; Predicted glutamine amidotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87081871, Length=253, Percent_Identity=39.5256916996047, Blast_Score=189, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR011697 [H]

Pfam domain/function: PF07722 Peptidase_C26 [H]

EC number: =3.5.1.94 [H]

Molecular weight: Translated: 28056; Mature: 27925

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKPLVGITADKKMMGAHPSHVVVEKYVTAVVDGADALAMLVPALGARQAPGDVLAAID
CCCCCEEEEECCCHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHC
GLLLTGSYSNVEPHRYGGEPSAPGTLHDPARDETALALARAAIDAGVPVLAICRGLQEMN
CEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VVFGGTLHQQVHAAGGFDDHREDKRDTLDAQYAPAHEIRLAEGGLLRRLLGGAAHARVNS
HHHCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHEEECCCHHHHHHHHHHHHHHHHH
LHGQGIAQLGAGLAIEALAPDGLIEAVRVPHARAFALGVQWHPEWRHANDALSTAIFRAF
HCCCCHHHHCCCEEEEECCCCHHHHHHHCCCCEEEEEECEECCCHHCHHHHHHHHHHHHH
GDACRERMRIRMRHDAGRLPTHAIGA
HHHHHHHHHHHHHHCCCCCCCHHCCC
>Mature Secondary Structure 
SSKPLVGITADKKMMGAHPSHVVVEKYVTAVVDGADALAMLVPALGARQAPGDVLAAID
CCCCEEEEECCCHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHC
GLLLTGSYSNVEPHRYGGEPSAPGTLHDPARDETALALARAAIDAGVPVLAICRGLQEMN
CEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VVFGGTLHQQVHAAGGFDDHREDKRDTLDAQYAPAHEIRLAEGGLLRRLLGGAAHARVNS
HHHCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHEEECCCHHHHHHHHHHHHHHHHH
LHGQGIAQLGAGLAIEALAPDGLIEAVRVPHARAFALGVQWHPEWRHANDALSTAIFRAF
HCCCCHHHHCCCEEEEECCCCHHHHHHHCCCCEEEEEECEECCCHHCHHHHHHHHHHHHH
GDACRERMRIRMRHDAGRLPTHAIGA
HHHHHHHHHHHHHHCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]