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The map label for this gene is puuA [H]

Identifier: 53721506

GI number: 53721506

Start: 639178

End: 640512

Strand: Reverse

Name: puuA [H]

Synonym: BPSS0469

Alternate gene names: 53721506

Gene position: 640512-639178 (Counterclockwise)

Preceding gene: 53721507

Following gene: 53721505

Centisome position: 20.19

GC content: 64.27

Gene sequence:

>1335_bases
ATGCAAGACATCGACGAATTCCTGAAGAAACACCGGATCACCGAGATCGAGGCGATCATCCCCGACATGGCCGGCATCGC
GCGCGGCAAGATCATTCCGCGCAGCAAGTTCGAAACCGGCGAATCGATGCGCCTGCCGCAGGCGGTGATGATTCAGACCG
TCACCGGCGATTATCCGGAAGACGGCAGCCTGACGGGCGTCACCGACCCCGACATGGTGTGCCTGCCCGATGCGTCGACG
ATCCGCCTGATTCCATGGGCGATCGATCCGACCGCGCAGGTGATCCACGATTGCGTGCATTTCGACGGCACGCCCGTCGA
GATCTCGCCGCGCCGGGTGCTGCGCCGCGTGCTCGAGCTCTACAAGGCGAAAGGCTGGAAGCCGGTGATCGCGCCGGAGC
TCGAGTTCTACCTGGTCGACATGAACAAGGACCCGGACCTGCCGCTCGCGCCGCCCATCGGCCGCACCGGCCGGCCCGAG
ACGGGACGCCAGTCGTATTCGATCGAAGCGGTCAACGAGTTCGATCCGCTTTTCGAAGACATCTACGAATACTGCGAAGT
GCAGGATCTCGAAGTGGACACGCTGATTCACGAAGTCGGCGCGGCGCAAATGGAGATCAACTTCCTGCACGGCGATCCGC
TGAATCTCGCCGACCGCGTGTTCCTCTTCAAGCGCACCGTGCGCGAAGCGGCGCTGCGGCACCACATGTACGCGACGTTC
ATGTCCAAGCCGATGGAGAACGAGCCGGGCTCGGCGATGCACGTGCATCAGAGCATCGTGGACGAGGAGACCGGCCGCAA
TCTGTTCACCGGCGACGACGGCGGCCCGACCGACATGTTCTACGCGTACATCGCCGGGCTGCAGAAGTACACGCCCGCGC
TGATGCCGATCTTCGCGCCGTACATCAACTCGTACCGGCGGCTGTCGCGCTTCATGGCCGCGCCGATCAACGTGCAGTGG
GGCTACGACAACCGCACGGTCGGCTTCCGGGTTCCGCATTCGTCCCCGGTGTCGCGCCGCGTCGAGAACCGGATTCCGGG
CGTCGACTGCAACCCGTACCTCGCGATCGCGGGCACGCTCGCGGCGGGCTACCTCGGCATGACGCGGCGCCTCGAGCCGA
CCGAGCCGCTCGCGAGCGACGGCTACAGCCTGCCGTACCAGTTGCCGCGCAATCTGGAGGAAGGGCTCACGCTGATGAGC
GCGTGCGAGCCGCTGGCGGAAATCCTCGGCGAGAAGTTCGTGAAGGCTTATCTCGCGCTGAAGGAAACCGAATACGAAGC
GTTCTTCCGCGTGATCAGCTCGTGGGAACGCAAGCATCTGCTGCTGCACGTCTGA

Upstream 100 bases:

>100_bases
CGTGCCGCGAACGGATGCGCATCCGCATGCGGCACGACGCCGGCCGGCTGCCCACGCACGCGATCGGCGCGTAAACCGTC
CCACTACAACGAGAACGATC

Downstream 100 bases:

>100_bases
ACGCGCATGCAGCCGCATCCATCCAGGAGGACACGATGAGCTACCGACCGAGCAACGCATCCGCATGGATTCAACCCGCC
GCGCCCGCGCACCGCACGAG

Product: glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MQDIDEFLKKHRITEIEAIIPDMAGIARGKIIPRSKFETGESMRLPQAVMIQTVTGDYPEDGSLTGVTDPDMVCLPDAST
IRLIPWAIDPTAQVIHDCVHFDGTPVEISPRRVLRRVLELYKAKGWKPVIAPELEFYLVDMNKDPDLPLAPPIGRTGRPE
TGRQSYSIEAVNEFDPLFEDIYEYCEVQDLEVDTLIHEVGAAQMEINFLHGDPLNLADRVFLFKRTVREAALRHHMYATF
MSKPMENEPGSAMHVHQSIVDEETGRNLFTGDDGGPTDMFYAYIAGLQKYTPALMPIFAPYINSYRRLSRFMAAPINVQW
GYDNRTVGFRVPHSSPVSRRVENRIPGVDCNPYLAIAGTLAAGYLGMTRRLEPTEPLASDGYSLPYQLPRNLEEGLTLMS
ACEPLAEILGEKFVKAYLALKETEYEAFFRVISSWERKHLLLHV

Sequences:

>Translated_444_residues
MQDIDEFLKKHRITEIEAIIPDMAGIARGKIIPRSKFETGESMRLPQAVMIQTVTGDYPEDGSLTGVTDPDMVCLPDAST
IRLIPWAIDPTAQVIHDCVHFDGTPVEISPRRVLRRVLELYKAKGWKPVIAPELEFYLVDMNKDPDLPLAPPIGRTGRPE
TGRQSYSIEAVNEFDPLFEDIYEYCEVQDLEVDTLIHEVGAAQMEINFLHGDPLNLADRVFLFKRTVREAALRHHMYATF
MSKPMENEPGSAMHVHQSIVDEETGRNLFTGDDGGPTDMFYAYIAGLQKYTPALMPIFAPYINSYRRLSRFMAAPINVQW
GYDNRTVGFRVPHSSPVSRRVENRIPGVDCNPYLAIAGTLAAGYLGMTRRLEPTEPLASDGYSLPYQLPRNLEEGLTLMS
ACEPLAEILGEKFVKAYLALKETEYEAFFRVISSWERKHLLLHV
>Mature_444_residues
MQDIDEFLKKHRITEIEAIIPDMAGIARGKIIPRSKFETGESMRLPQAVMIQTVTGDYPEDGSLTGVTDPDMVCLPDAST
IRLIPWAIDPTAQVIHDCVHFDGTPVEISPRRVLRRVLELYKAKGWKPVIAPELEFYLVDMNKDPDLPLAPPIGRTGRPE
TGRQSYSIEAVNEFDPLFEDIYEYCEVQDLEVDTLIHEVGAAQMEINFLHGDPLNLADRVFLFKRTVREAALRHHMYATF
MSKPMENEPGSAMHVHQSIVDEETGRNLFTGDDGGPTDMFYAYIAGLQKYTPALMPIFAPYINSYRRLSRFMAAPINVQW
GYDNRTVGFRVPHSSPVSRRVENRIPGVDCNPYLAIAGTLAAGYLGMTRRLEPTEPLASDGYSLPYQLPRNLEEGLTLMS
ACEPLAEILGEKFVKAYLALKETEYEAFFRVISSWERKHLLLHV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=444, Percent_Identity=25.9009009009009, Blast_Score=143, Evalue=4e-34,
Organism=Escherichia coli, GI87081870, Length=450, Percent_Identity=33.3333333333333, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1790301, Length=403, Percent_Identity=29.2803970223325, Blast_Score=138, Evalue=8e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 50194; Mature: 50194

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDIDEFLKKHRITEIEAIIPDMAGIARGKIIPRSKFETGESMRLPQAVMIQTVTGDYPE
CCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHEEEEECCCCCC
DGSLTGVTDPDMVCLPDASTIRLIPWAIDPTAQVIHDCVHFDGTPVEISPRRVLRRVLEL
CCCCCCCCCCCEEEECCCCEEEEEEEECCHHHHHHHHHHHCCCCCEEECHHHHHHHHHHH
YKAKGWKPVIAPELEFYLVDMNKDPDLPLAPPIGRTGRPETGRQSYSIEAVNEFDPLFED
HHCCCCCCEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHH
IYEYCEVQDLEVDTLIHEVGAAQMEINFLHGDPLNLADRVFLFKRTVREAALRHHMYATF
HHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MSKPMENEPGSAMHVHQSIVDEETGRNLFTGDDGGPTDMFYAYIAGLQKYTPALMPIFAP
HHCCCCCCCCCHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHCHHHHHHHHH
YINSYRRLSRFMAAPINVQWGYDNRTVGFRVPHSSPVSRRVENRIPGVDCNPYLAIAGTL
HHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCHHHHHHHH
AAGYLGMTRRLEPTEPLASDGYSLPYQLPRNLEEGLTLMSACEPLAEILGEKFVKAYLAL
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KETEYEAFFRVISSWERKHLLLHV
HHHHHHHHHHHHHHHHHCEEEEEC
>Mature Secondary Structure
MQDIDEFLKKHRITEIEAIIPDMAGIARGKIIPRSKFETGESMRLPQAVMIQTVTGDYPE
CCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHEEEEECCCCCC
DGSLTGVTDPDMVCLPDASTIRLIPWAIDPTAQVIHDCVHFDGTPVEISPRRVLRRVLEL
CCCCCCCCCCCEEEECCCCEEEEEEEECCHHHHHHHHHHHCCCCCEEECHHHHHHHHHHH
YKAKGWKPVIAPELEFYLVDMNKDPDLPLAPPIGRTGRPETGRQSYSIEAVNEFDPLFED
HHCCCCCCEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHH
IYEYCEVQDLEVDTLIHEVGAAQMEINFLHGDPLNLADRVFLFKRTVREAALRHHMYATF
HHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MSKPMENEPGSAMHVHQSIVDEETGRNLFTGDDGGPTDMFYAYIAGLQKYTPALMPIFAP
HHCCCCCCCCCHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHCHHHHHHHHH
YINSYRRLSRFMAAPINVQWGYDNRTVGFRVPHSSPVSRRVENRIPGVDCNPYLAIAGTL
HHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCHHHHHHHH
AAGYLGMTRRLEPTEPLASDGYSLPYQLPRNLEEGLTLMSACEPLAEILGEKFVKAYLAL
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KETEYEAFFRVISSWERKHLLLHV
HHHHHHHHHHHHHHHHHCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]