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The map label for this gene is dppA [H]

Identifier: 53718016

GI number: 53718016

Start: 407612

End: 409198

Strand: Reverse

Name: dppA [H]

Synonym: BPSL0376

Alternate gene names: 53718016

Gene position: 409198-407612 (Counterclockwise)

Preceding gene: 53718017

Following gene: 53718015

Centisome position: 10.04

GC content: 65.66

Gene sequence:

>1587_bases
ATGCGCTTCACGCACATCGCCGCCGCCGCGCTCGTCGCCGCCGCTTCCGTCGCCGCCGCGAAACCGCTGACGGTCTGCAC
CGAGTCGAGCCCGGACGGCTTCGACGTCGTTCAGTACAACTCGCTCGTCACGACGAACGCATCCGCCGACGTCGTCTTCA
ACACGCTCGTGTCGTACGACGAAGCGACGAAAAAGGTGGTGCCCGCGCTCGCGGACAAATGGGATGTGAGCGCCGACGGC
CTCGCGTACACGTTCCATCTGCGCCCGAACGTCGCGTTCCAGACCACCGACTCCTTCAAGCCGACGCGCGCGCTCGACGC
CGACGACGTCGTCTTCACGTTCGGCCGGATGCTCGACGACGCGAATCCGTGGCACAAGGTCGCGGGCGCGAGCGGCTTTC
CGCATGCGCAGTCGATGGGCCTCGCGAAGCTCGTGAAGGCGGTCACGAAGATCGACGAGTCGACCGTGAAGTTCGAACTG
AACGAACCGAACGCGACGTTCGTGCCGATCCTCACGATGGGCTTCGCGTCGATCTACTCGGCCGAGTACGCGGATCAGCT
GCTGAAGGCGGGCAAGCAGGCGGACTTGAACGCGAAGCCGGTCGGCACCGGGCCGTTCGTGCTGAAGAGTTACACGAAGG
ACGCGGTGATCCGCTACGAGGCGAACCCGACGTACTGGGGGCCGAAGCCGAAGGTCGAGCGCCTCATCTACGCGATCACG
CCCGATCCGTCGGTGCGCGCGCAGAAGGTGAAGGCGGGCGAATGCCAGATCGCGCTGTCGCCGAAGCCGCAGGACGTCGC
GGCCGCGAAGGCCGACCGCGCGCTGAGGGTCGTCGAGACGCCCGCGTTCATGACCGCGTTCGTCGCGCTCAACACGCAGA
AGAAACCGCTCGACAACGACAAGGTTCGCGAGGCGCTCAATCTCGCGTTCGATCGCGCGACGTACCTGAAGGTCGTGTTC
GACAACACGGCGACGCCCGCGACGAATCCGTATCCGCCGAACACGTGGAGCTACGCGAAATCGATCGCGCCGTATCCGCA
CGATCCGGCGAAGGCGAAGCAGTTGCTCGCGCAGGCGGGCTTCCCGAACGGCTTCTCGACGACGATCTGGGTGCGCCCGA
CGGGCAGCGTGCTGAACCCGAACCCGAAGGCGGGCGCGGAGCTGCTGCAGGCCGACCTCGCGAAGATCGGCGTGAAGGCG
GACGTGCGGGTGATCGAATGGGGCGAGCTGATCAAGCAGGCGAAGCTCGGCCAGCACGATCTGCTGTTCATGGGCTTCGC
CGGCGACAACGGCGATCCGGACAACATCCTCACGCCGCAATTCAGTTGCAACTCGGTGAAGTCGGGCCTGAACTTCGCGC
GCTATTGCGATCCGAAGCTCGACAAGCTGATCGCCGACGCAAAGGAAACCGCCGATCAAGCCAAGCGCGCGAAGCTGTAC
GAGGCCGCGCAGAAGATCATCCACGACGAGGCGCTGTGGATTCCCCTCGGCTATCCGACCGCCGCGGCGATTACCCGGCC
GAACGTCGCCGGCTATCGCGTGAGCCCGTTCGGGCGGCAGCACTTCGACGCGGTCTCGGTGCAGTAA

Upstream 100 bases:

>100_bases
TAACGGCGAGCGCCCATTGACGGTATGCTATCCCGCTGGCGTGCGGCCGCTGCGGCGTTGCCGCCCGCCGCCGACAGAAT
TACGCCACGTGGGAGATCTC

Downstream 100 bases:

>100_bases
CGCCGCGGCACACGCCGCCGCGGCCCCGGCGCAACACCGCGCCGGGGCCGCGCTTGTCCGCATCGCGCGACGCTTGCTAT
ACTCCGCGCACCTTTCCTCC

Product: extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MRFTHIAAAALVAAASVAAAKPLTVCTESSPDGFDVVQYNSLVTTNASADVVFNTLVSYDEATKKVVPALADKWDVSADG
LAYTFHLRPNVAFQTTDSFKPTRALDADDVVFTFGRMLDDANPWHKVAGASGFPHAQSMGLAKLVKAVTKIDESTVKFEL
NEPNATFVPILTMGFASIYSAEYADQLLKAGKQADLNAKPVGTGPFVLKSYTKDAVIRYEANPTYWGPKPKVERLIYAIT
PDPSVRAQKVKAGECQIALSPKPQDVAAAKADRALRVVETPAFMTAFVALNTQKKPLDNDKVREALNLAFDRATYLKVVF
DNTATPATNPYPPNTWSYAKSIAPYPHDPAKAKQLLAQAGFPNGFSTTIWVRPTGSVLNPNPKAGAELLQADLAKIGVKA
DVRVIEWGELIKQAKLGQHDLLFMGFAGDNGDPDNILTPQFSCNSVKSGLNFARYCDPKLDKLIADAKETADQAKRAKLY
EAAQKIIHDEALWIPLGYPTAAAITRPNVAGYRVSPFGRQHFDAVSVQ

Sequences:

>Translated_528_residues
MRFTHIAAAALVAAASVAAAKPLTVCTESSPDGFDVVQYNSLVTTNASADVVFNTLVSYDEATKKVVPALADKWDVSADG
LAYTFHLRPNVAFQTTDSFKPTRALDADDVVFTFGRMLDDANPWHKVAGASGFPHAQSMGLAKLVKAVTKIDESTVKFEL
NEPNATFVPILTMGFASIYSAEYADQLLKAGKQADLNAKPVGTGPFVLKSYTKDAVIRYEANPTYWGPKPKVERLIYAIT
PDPSVRAQKVKAGECQIALSPKPQDVAAAKADRALRVVETPAFMTAFVALNTQKKPLDNDKVREALNLAFDRATYLKVVF
DNTATPATNPYPPNTWSYAKSIAPYPHDPAKAKQLLAQAGFPNGFSTTIWVRPTGSVLNPNPKAGAELLQADLAKIGVKA
DVRVIEWGELIKQAKLGQHDLLFMGFAGDNGDPDNILTPQFSCNSVKSGLNFARYCDPKLDKLIADAKETADQAKRAKLY
EAAQKIIHDEALWIPLGYPTAAAITRPNVAGYRVSPFGRQHFDAVSVQ
>Mature_528_residues
MRFTHIAAAALVAAASVAAAKPLTVCTESSPDGFDVVQYNSLVTTNASADVVFNTLVSYDEATKKVVPALADKWDVSADG
LAYTFHLRPNVAFQTTDSFKPTRALDADDVVFTFGRMLDDANPWHKVAGASGFPHAQSMGLAKLVKAVTKIDESTVKFEL
NEPNATFVPILTMGFASIYSAEYADQLLKAGKQADLNAKPVGTGPFVLKSYTKDAVIRYEANPTYWGPKPKVERLIYAIT
PDPSVRAQKVKAGECQIALSPKPQDVAAAKADRALRVVETPAFMTAFVALNTQKKPLDNDKVREALNLAFDRATYLKVVF
DNTATPATNPYPPNTWSYAKSIAPYPHDPAKAKQLLAQAGFPNGFSTTIWVRPTGSVLNPNPKAGAELLQADLAKIGVKA
DVRVIEWGELIKQAKLGQHDLLFMGFAGDNGDPDNILTPQFSCNSVKSGLNFARYCDPKLDKLIADAKETADQAKRAKLY
EAAQKIIHDEALWIPLGYPTAAAITRPNVAGYRVSPFGRQHFDAVSVQ

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=523, Percent_Identity=43.5946462715105, Blast_Score=474, Evalue=1e-135,
Organism=Escherichia coli, GI1787551, Length=532, Percent_Identity=34.3984962406015, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI1787052, Length=516, Percent_Identity=31.3953488372093, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1789887, Length=496, Percent_Identity=26.008064516129, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1787762, Length=482, Percent_Identity=25.7261410788382, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1787495, Length=529, Percent_Identity=24.7637051039698, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI87081878, Length=481, Percent_Identity=25.7796257796258, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1789397, Length=539, Percent_Identity=25.417439703154, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57192; Mature: 57192

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFTHIAAAALVAAASVAAAKPLTVCTESSPDGFDVVQYNSLVTTNASADVVFNTLVSYD
CCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEECCEEECCCCHHHHHHHHHHHH
EATKKVVPALADKWDVSADGLAYTFHLRPNVAFQTTDSFKPTRALDADDVVFTFGRMLDD
HHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCC
ANPWHKVAGASGFPHAQSMGLAKLVKAVTKIDESTVKFELNEPNATFVPILTMGFASIYS
CCCHHHHCCCCCCCCHHHCCHHHHHHHHHHCCCCEEEEEECCCCCEEHHHHHHHHHHHHH
AEYADQLLKAGKQADLNAKPVGTGPFVLKSYTKDAVIRYEANPTYWGPKPKVERLIYAIT
HHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEEEEC
PDPSVRAQKVKAGECQIALSPKPQDVAAAKADRALRVVETPAFMTAFVALNTQKKPLDND
CCCCCCHHEECCCCEEEEECCCCCHHHHHHHCCCEEEEECCHHHHHHHEECCCCCCCCHH
KVREALNLAFDRATYLKVVFDNTATPATNPYPPNTWSYAKSIAPYPHDPAKAKQLLAQAG
HHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHCC
FPNGFSTTIWVRPTGSVLNPNPKAGAELLQADLAKIGVKADVRVIEWGELIKQAKLGQHD
CCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHCCCCC
LLFMGFAGDNGDPDNILTPQFSCNSVKSGLNFARYCDPKLDKLIADAKETADQAKRAKLY
EEEEEECCCCCCCCCEECCCCCHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
EAAQKIIHDEALWIPLGYPTAAAITRPNVAGYRVSPFGRQHFDAVSVQ
HHHHHHHCCCEEEEECCCCCHHHEECCCCCCEEECCCCCCCCCEECCC
>Mature Secondary Structure
MRFTHIAAAALVAAASVAAAKPLTVCTESSPDGFDVVQYNSLVTTNASADVVFNTLVSYD
CCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEECCEEECCCCHHHHHHHHHHHH
EATKKVVPALADKWDVSADGLAYTFHLRPNVAFQTTDSFKPTRALDADDVVFTFGRMLDD
HHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCC
ANPWHKVAGASGFPHAQSMGLAKLVKAVTKIDESTVKFELNEPNATFVPILTMGFASIYS
CCCHHHHCCCCCCCCHHHCCHHHHHHHHHHCCCCEEEEEECCCCCEEHHHHHHHHHHHHH
AEYADQLLKAGKQADLNAKPVGTGPFVLKSYTKDAVIRYEANPTYWGPKPKVERLIYAIT
HHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEEEEC
PDPSVRAQKVKAGECQIALSPKPQDVAAAKADRALRVVETPAFMTAFVALNTQKKPLDND
CCCCCCHHEECCCCEEEEECCCCCHHHHHHHCCCEEEEECCHHHHHHHEECCCCCCCCHH
KVREALNLAFDRATYLKVVFDNTATPATNPYPPNTWSYAKSIAPYPHDPAKAKQLLAQAG
HHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHCC
FPNGFSTTIWVRPTGSVLNPNPKAGAELLQADLAKIGVKADVRVIEWGELIKQAKLGQHD
CCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHCCCCC
LLFMGFAGDNGDPDNILTPQFSCNSVKSGLNFARYCDPKLDKLIADAKETADQAKRAKLY
EEEEEECCCCCCCCCEECCCCCHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
EAAQKIIHDEALWIPLGYPTAAAITRPNVAGYRVSPFGRQHFDAVSVQ
HHHHHHHCCCEEEEECCCCCHHHEECCCCCCEEECCCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]