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The map label for this gene is bioF

Identifier: 53718006

GI number: 53718006

Start: 394470

End: 395654

Strand: Reverse

Name: bioF

Synonym: BPSL0366

Alternate gene names: 53718006

Gene position: 395654-394470 (Counterclockwise)

Preceding gene: 53718007

Following gene: 53718005

Centisome position: 9.71

GC content: 73.33

Gene sequence:

>1185_bases
ATGAATCCGCTCGCCACGCTCGAACAAGGCCTCGCCGACATCGACGCGCAAGGGCTGCGCCGGTGCCGCCGCGTCGCGGA
CACCGCGTGCGGCGCGCACATGACGGTGGACGGCCGCGCAATCATCGGCTTCGCGAGCAACGACTATCTCGGGCTCGCCG
CGCATCCGCGCCTCGTCGAAGCGTTCGCCGAAGGCGCGCGCCGCTACGGCTCGGGCAGCGGCGGCTCGCATCTGCTCGGC
GGCCACTCGCGCGCGCACGCGACGCTCGAAGACGAGCTCGCCGCGTTCTCCGGCGGCTTCTCCGATGCGCCGCGCGCGCT
GTACTTCAGCACCGGCTACATGGCGAACCTCGCCGCGCTCACCGCGCTCGCGGGCCGCGGCGCGACGATCTTCTCCGACG
CGCTCAATCATGCGTCGCTGATCGACGGCGCGCGGTTGTCGCGCGCGAACGTGCAGATCTACCCGCACGGCGACGCCGAC
GCGCTCGACGCGCGCCTGCGCGCCTGCGACGCGCCGACGAAGCTGATCGTCTCCGACACCGTGTTCAGCATGGACGGCGA
CGTCGCGCCGCTCGCGCGCCTCGTCGCGCTCGCCGAGACACACGGCGCGTGGCTCGTCGTCGACGACGCGCACGGCTTCG
GCGTGCTCGGCCCGCAAGGCCGCGGCGCGCTCGCCGCGCACGGGCTGCGCTCGCCGAACCTCGTCTACGTCGGCACGCTC
GGCAAGGCGGCGGGCGTCGCGGGCGCGTTCGTCGTCGCGCATGAAACGGTGATCGAATGGCTCGTGCAGCGCGCGCGCAG
CTATATCTTCACGACGGCCGCGCCGCCGTCCGTCGCGTGCGCGGTGTCCGCGAGCCTCGCGGTCATCGCGAGCGACGAAG
GCGACGCGCGCCGCGCGCATCTCGGCGCACTGATCAAGCGCACCCGTGCGATCCTGCGCGCCACGCATTGGCAGCCCGTC
GATTCGCACACGGCCGTGCAGCCGCTCGTGATCGGCAGCAACGAAGCGACGCTCGCGGCGATGGCCGCGCTCGACGCGCA
GGGCCTGTGGGTGCCCGCGATCCGGCCGCCGACGGTGCCGGCCGGCACGTCGCGGCTGCGCATCTCGCTGTCGGCCGCGC
ATTCGTTCGACGATCTCGCACGCCTCGAGGCGGCGCTCGTCACGCCGATCGGAGCCGCCGCATGA

Upstream 100 bases:

>100_bases
TCTACCTGATGCCGCCTTACATCCTCGACGAAGGCGAGATCGAATGGCTCGCCTCGCGCACGCGCGCGACGCTCGACGCA
ACGCTGTCGGAGGCCCACGC

Downstream 100 bases:

>100_bases
GCGCGCCGCTTTCGCTGTTCGTGACGGGCACCGATACCGAGATCGGCAAGACGTTCGTATCGGCCGCGCTGCTGCACGGC
TTCGCGCGCGCGGGGCTGCG

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MNPLATLEQGLADIDAQGLRRCRRVADTACGAHMTVDGRAIIGFASNDYLGLAAHPRLVEAFAEGARRYGSGSGGSHLLG
GHSRAHATLEDELAAFSGGFSDAPRALYFSTGYMANLAALTALAGRGATIFSDALNHASLIDGARLSRANVQIYPHGDAD
ALDARLRACDAPTKLIVSDTVFSMDGDVAPLARLVALAETHGAWLVVDDAHGFGVLGPQGRGALAAHGLRSPNLVYVGTL
GKAAGVAGAFVVAHETVIEWLVQRARSYIFTTAAPPSVACAVSASLAVIASDEGDARRAHLGALIKRTRAILRATHWQPV
DSHTAVQPLVIGSNEATLAAMAALDAQGLWVPAIRPPTVPAGTSRLRISLSAAHSFDDLARLEAALVTPIGAAA

Sequences:

>Translated_394_residues
MNPLATLEQGLADIDAQGLRRCRRVADTACGAHMTVDGRAIIGFASNDYLGLAAHPRLVEAFAEGARRYGSGSGGSHLLG
GHSRAHATLEDELAAFSGGFSDAPRALYFSTGYMANLAALTALAGRGATIFSDALNHASLIDGARLSRANVQIYPHGDAD
ALDARLRACDAPTKLIVSDTVFSMDGDVAPLARLVALAETHGAWLVVDDAHGFGVLGPQGRGALAAHGLRSPNLVYVGTL
GKAAGVAGAFVVAHETVIEWLVQRARSYIFTTAAPPSVACAVSASLAVIASDEGDARRAHLGALIKRTRAILRATHWQPV
DSHTAVQPLVIGSNEATLAAMAALDAQGLWVPAIRPPTVPAGTSRLRISLSAAHSFDDLARLEAALVTPIGAAA
>Mature_394_residues
MNPLATLEQGLADIDAQGLRRCRRVADTACGAHMTVDGRAIIGFASNDYLGLAAHPRLVEAFAEGARRYGSGSGGSHLLG
GHSRAHATLEDELAAFSGGFSDAPRALYFSTGYMANLAALTALAGRGATIFSDALNHASLIDGARLSRANVQIYPHGDAD
ALDARLRACDAPTKLIVSDTVFSMDGDVAPLARLVALAETHGAWLVVDDAHGFGVLGPQGRGALAAHGLRSPNLVYVGTL
GKAAGVAGAFVVAHETVIEWLVQRARSYIFTTAAPPSVACAVSASLAVIASDEGDARRAHLGALIKRTRAILRATHWQPV
DSHTAVQPLVIGSNEATLAAMAALDAQGLWVPAIRPPTVPAGTSRLRISLSAAHSFDDLARLEAALVTPIGAAA

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily

Homologues:

Organism=Homo sapiens, GI7657118, Length=387, Percent_Identity=32.8165374677003, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI284448556, Length=363, Percent_Identity=32.2314049586777, Blast_Score=184, Evalue=9e-47,
Organism=Homo sapiens, GI83977442, Length=349, Percent_Identity=35.243553008596, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI83977444, Length=349, Percent_Identity=35.243553008596, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI83977440, Length=349, Percent_Identity=35.243553008596, Blast_Score=179, Evalue=4e-45,
Organism=Homo sapiens, GI4502025, Length=358, Percent_Identity=33.5195530726257, Blast_Score=165, Evalue=6e-41,
Organism=Homo sapiens, GI40316939, Length=358, Percent_Identity=33.5195530726257, Blast_Score=165, Evalue=6e-41,
Organism=Homo sapiens, GI4758668, Length=360, Percent_Identity=29.4444444444444, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI119220554, Length=367, Percent_Identity=27.7929155313352, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI5454084, Length=276, Percent_Identity=27.1739130434783, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1786993, Length=353, Percent_Identity=46.742209631728, Blast_Score=279, Evalue=2e-76,
Organism=Escherichia coli, GI1790046, Length=382, Percent_Identity=35.8638743455497, Blast_Score=202, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI71994529, Length=393, Percent_Identity=34.0966921119593, Blast_Score=190, Evalue=9e-49,
Organism=Caenorhabditis elegans, GI32566772, Length=363, Percent_Identity=26.4462809917355, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17560914, Length=364, Percent_Identity=26.3736263736264, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17560912, Length=364, Percent_Identity=26.3736263736264, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71982617, Length=286, Percent_Identity=25.5244755244755, Blast_Score=98, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI71982625, Length=286, Percent_Identity=25.5244755244755, Blast_Score=98, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6320438, Length=410, Percent_Identity=31.219512195122, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6320267, Length=358, Percent_Identity=27.0949720670391, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6323954, Length=271, Percent_Identity=28.4132841328413, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24662918, Length=383, Percent_Identity=32.3759791122715, Blast_Score=195, Evalue=4e-50,
Organism=Drosophila melanogaster, GI17137420, Length=354, Percent_Identity=31.9209039548023, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI17136286, Length=357, Percent_Identity=26.0504201680672, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24653280, Length=325, Percent_Identity=26.1538461538462, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24653276, Length=325, Percent_Identity=26.1538461538462, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24653278, Length=325, Percent_Identity=26.1538461538462, Blast_Score=109, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOF_BURM7 (A3MNG3)

Other databases:

- EMBL:   CP000548
- RefSeq:   YP_001081799.1
- ProteinModelPortal:   A3MNG3
- GeneID:   4892747
- GenomeReviews:   CP000548_GR
- KEGG:   bmn:BMA10247_2271
- TIGR:   BMA10247_2271
- HOGENOM:   HBG649839
- OMA:   REGSHIT
- ProtClustDB:   PRK05958
- BioCyc:   BMAL320389:BMA10247_2271-MONOMER
- HAMAP:   MF_01693
- InterPro:   IPR022834
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR00858

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.3.1.47

Molecular weight: Translated: 40700; Mature: 40700

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: BINDING 21-21 BINDING 137-137 BINDING 183-183 BINDING 358-358

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPLATLEQGLADIDAQGLRRCRRVADTACGAHMTVDGRAIIGFASNDYLGLAAHPRLVE
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEECCEEEEEECCCCEEEECCCHHHHH
AFAEGARRYGSGSGGSHLLGGHSRAHATLEDELAAFSGGFSDAPRALYFSTGYMANLAAL
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHH
TALAGRGATIFSDALNHASLIDGARLSRANVQIYPHGDADALDARLRACDAPTKLIVSDT
HHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCEEEEECE
VFSMDGDVAPLARLVALAETHGAWLVVDDAHGFGVLGPQGRGALAAHGLRSPNLVYVGTL
EEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCHHHCCCCCCCEEEEECC
GKAAGVAGAFVVAHETVIEWLVQRARSYIFTTAAPPSVACAVSASLAVIASDEGDARRAH
CCHHCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEECEEEEEECCCCCHHHHH
LGALIKRTRAILRATHWQPVDSHTAVQPLVIGSNEATLAAMAALDAQGLWVPAIRPPTVP
HHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCEECCCCCCCCC
AGTSRLRISLSAAHSFDDLARLEAALVTPIGAAA
CCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNPLATLEQGLADIDAQGLRRCRRVADTACGAHMTVDGRAIIGFASNDYLGLAAHPRLVE
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEECCEEEEEECCCCEEEECCCHHHHH
AFAEGARRYGSGSGGSHLLGGHSRAHATLEDELAAFSGGFSDAPRALYFSTGYMANLAAL
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHH
TALAGRGATIFSDALNHASLIDGARLSRANVQIYPHGDADALDARLRACDAPTKLIVSDT
HHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCEEEEECE
VFSMDGDVAPLARLVALAETHGAWLVVDDAHGFGVLGPQGRGALAAHGLRSPNLVYVGTL
EEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCHHHCCCCCCCEEEEECC
GKAAGVAGAFVVAHETVIEWLVQRARSYIFTTAAPPSVACAVSASLAVIASDEGDARRAH
CCHHCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEECEEEEEECCCCCHHHHH
LGALIKRTRAILRATHWQPVDSHTAVQPLVIGSNEATLAAMAALDAQGLWVPAIRPPTVP
HHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCEECCCCCCCCC
AGTSRLRISLSAAHSFDDLARLEAALVTPIGAAA
CCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA