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The map label for this gene is proP1

Identifier: 52842599

GI number: 52842599

Start: 2700982

End: 2702220

Strand: Reverse

Name: proP1

Synonym: lpg2390

Alternate gene names: 52842599

Gene position: 2702220-2700982 (Counterclockwise)

Preceding gene: 52842600

Following gene: 52842597

Centisome position: 79.53

GC content: 38.9

Gene sequence:

>1239_bases
TTGGATATGGATAAAAGGATTATTGCGGCAGCCTCTTTTGGTACCTTGTTTGAAATGTACGACTATGCCATTTATGGGTT
TATGGCGCCTATTTTGGCGCCGCTTTTTTTCCCCACTACCGATAAACACACTGCTCTCATCGCAACATTTGGTATTTTCG
CGGCAGGTTTCCTTGTTCGGCCATTCTCTGCTTATTGGTTAGGGAATCTGGGTGACCAAATTGGTCGCAAAAAAACATTG
ACCATAACTCTTATGGTCATGACTTTTTCATCAGGTTTTATTGGTTTATTACCTACTTACCAAGAAGCCGGTATTTTTGC
TCCAATTTTGCTCACGTTCTGTCGATTAACTCAAGGGGTTTCTGTGGCAGGGGAAATGACCTTAGCCAGTATTTACATTT
ATGAAAATGTGAGCATGCGCCATAAAGGTTTAGCAGTCAGCTTTGTCACCTTGGGTTCGATTTGTGGGCTGTTGCTTGCA
ATAATAGTCAGCAAAATAACCATGCTGTTTTTTTCCAAACAGCATCTCGATTCCTTCTGGCGCTTCCCATTTTTAATAGG
AATATTCGTAGGTTTTGCCGGACTTTATTTGCGAAGTCAAACCAGAGAATTAACCGATTTTGCAAAAGTAGTTGCTCAAA
GACAATCTCATCCCATATTAAATGCCTGGCATTATCGGAAAAAAGCAATTGCTGCTATTTTTCTTTTGGCAATTTATTGC
AACAGTAGCTTTTATACATTTTTTGTTTATATGCCTTCATACCTGCAACAGCAGGAGTTGCCATTAACTACAGCGATGGA
TGTTATTATCATAGGGATGATTTGTTATAGCATGGGTCTCTTATTATCAGGTTTCACCTCTGATATTTTAGGCAGACGCT
TTCCCTTGGCGGTGAGTTTAATTGCAACTTTATGTACGGTTTTGGTAATAAATTATTTGATCAATACAGGTACTTTATTA
CAAATTACTGTCGCCTGCAGTGTTTTGGCTGTCATTATCTCTTTTTTTATTGGAAGTGCATTTACCGCCAGCCTTGAACA
ATTACCACAGGAAGAGCGGGCCAGTGGCTTTACTTTATTTTTTAACTTGTCTAATTCCCTATTTGGAGGCACAGCACCAC
TCATTATTTTGCTATTAAGTTATGCTTTCATCCATTTTGCATTTTCTATTTATTTGATGGTGATGGCTTTGTTGACTCTT
CCTGCCCTGTGGTTGATTCAAGAAAAAAGCACGAATTAA

Upstream 100 bases:

>100_bases
GTACACCTGTCATAGAGGAGGTGGAGACAGAGAAGCAAGAGCAGAAATCAATTGAGTTAAAGTAGGCTGTACCACTTACC
CGTAAAACAAAAACAACAGA

Downstream 100 bases:

>100_bases
TTTATTAGTTTATGGTACGAAAAACAGTGATCTAACACAAAAATCCAGCCAATATATTTATGTGGAAAAAAAGAGATAGA
CGGTATTGATACAGAAATTA

Product: proline/betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MDMDKRIIAAASFGTLFEMYDYAIYGFMAPILAPLFFPTTDKHTALIATFGIFAAGFLVRPFSAYWLGNLGDQIGRKKTL
TITLMVMTFSSGFIGLLPTYQEAGIFAPILLTFCRLTQGVSVAGEMTLASIYIYENVSMRHKGLAVSFVTLGSICGLLLA
IIVSKITMLFFSKQHLDSFWRFPFLIGIFVGFAGLYLRSQTRELTDFAKVVAQRQSHPILNAWHYRKKAIAAIFLLAIYC
NSSFYTFFVYMPSYLQQQELPLTTAMDVIIIGMICYSMGLLLSGFTSDILGRRFPLAVSLIATLCTVLVINYLINTGTLL
QITVACSVLAVIISFFIGSAFTASLEQLPQEERASGFTLFFNLSNSLFGGTAPLIILLLSYAFIHFAFSIYLMVMALLTL
PALWLIQEKSTN

Sequences:

>Translated_412_residues
MDMDKRIIAAASFGTLFEMYDYAIYGFMAPILAPLFFPTTDKHTALIATFGIFAAGFLVRPFSAYWLGNLGDQIGRKKTL
TITLMVMTFSSGFIGLLPTYQEAGIFAPILLTFCRLTQGVSVAGEMTLASIYIYENVSMRHKGLAVSFVTLGSICGLLLA
IIVSKITMLFFSKQHLDSFWRFPFLIGIFVGFAGLYLRSQTRELTDFAKVVAQRQSHPILNAWHYRKKAIAAIFLLAIYC
NSSFYTFFVYMPSYLQQQELPLTTAMDVIIIGMICYSMGLLLSGFTSDILGRRFPLAVSLIATLCTVLVINYLINTGTLL
QITVACSVLAVIISFFIGSAFTASLEQLPQEERASGFTLFFNLSNSLFGGTAPLIILLLSYAFIHFAFSIYLMVMALLTL
PALWLIQEKSTN
>Mature_412_residues
MDMDKRIIAAASFGTLFEMYDYAIYGFMAPILAPLFFPTTDKHTALIATFGIFAAGFLVRPFSAYWLGNLGDQIGRKKTL
TITLMVMTFSSGFIGLLPTYQEAGIFAPILLTFCRLTQGVSVAGEMTLASIYIYENVSMRHKGLAVSFVTLGSICGLLLA
IIVSKITMLFFSKQHLDSFWRFPFLIGIFVGFAGLYLRSQTRELTDFAKVVAQRQSHPILNAWHYRKKAIAAIFLLAIYC
NSSFYTFFVYMPSYLQQQELPLTTAMDVIIIGMICYSMGLLLSGFTSDILGRRFPLAVSLIATLCTVLVINYLINTGTLL
QITVACSVLAVIISFFIGSAFTASLEQLPQEERASGFTLFFNLSNSLFGGTAPLIILLLSYAFIHFAFSIYLMVMALLTL
PALWLIQEKSTN

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=412, Percent_Identity=33.252427184466, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1788942, Length=406, Percent_Identity=32.2660098522167, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1789941, Length=420, Percent_Identity=28.8095238095238, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1788292, Length=298, Percent_Identity=29.8657718120805, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45748; Mature: 45748

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDMDKRIIAAASFGTLFEMYDYAIYGFMAPILAPLFFPTTDKHTALIATFGIFAAGFLVR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
PFSAYWLGNLGDQIGRKKTLTITLMVMTFSSGFIGLLPTYQEAGIFAPILLTFCRLTQGV
HHHHHHHHHHHHHHCCCEEEEEEEEEEHHCCCCEECCCCHHHCCHHHHHHHHHHHHHCCC
SVAGEMTLASIYIYENVSMRHKGLAVSFVTLGSICGLLLAIIVSKITMLFFSKQHLDSFW
CHHCCHHEEEEEEEECCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFPFLIGIFVGFAGLYLRSQTRELTDFAKVVAQRQSHPILNAWHYRKKAIAAIFLLAIYC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
NSSFYTFFVYMPSYLQQQELPLTTAMDVIIIGMICYSMGLLLSGFTSDILGRRFPLAVSL
CCCCEEHEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IATLCTVLVINYLINTGTLLQITVACSVLAVIISFFIGSAFTASLEQLPQEERASGFTLF
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCEEEE
FNLSNSLFGGTAPLIILLLSYAFIHFAFSIYLMVMALLTLPALWLIQEKSTN
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MDMDKRIIAAASFGTLFEMYDYAIYGFMAPILAPLFFPTTDKHTALIATFGIFAAGFLVR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
PFSAYWLGNLGDQIGRKKTLTITLMVMTFSSGFIGLLPTYQEAGIFAPILLTFCRLTQGV
HHHHHHHHHHHHHHCCCEEEEEEEEEEHHCCCCEECCCCHHHCCHHHHHHHHHHHHHCCC
SVAGEMTLASIYIYENVSMRHKGLAVSFVTLGSICGLLLAIIVSKITMLFFSKQHLDSFW
CHHCCHHEEEEEEEECCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFPFLIGIFVGFAGLYLRSQTRELTDFAKVVAQRQSHPILNAWHYRKKAIAAIFLLAIYC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
NSSFYTFFVYMPSYLQQQELPLTTAMDVIIIGMICYSMGLLLSGFTSDILGRRFPLAVSL
CCCCEEHEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IATLCTVLVINYLINTGTLLQITVACSVLAVIISFFIGSAFTASLEQLPQEERASGFTLF
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCEEEE
FNLSNSLFGGTAPLIILLLSYAFIHFAFSIYLMVMALLTLPALWLIQEKSTN
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]