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The map label for this gene is pksJ [H]

Identifier: 52842156

GI number: 52842156

Start: 2166115

End: 2167431

Strand: Reverse

Name: pksJ [H]

Synonym: lpg1939

Alternate gene names: 52842156

Gene position: 2167431-2166115 (Counterclockwise)

Preceding gene: 52842157

Following gene: 52842154

Centisome position: 63.79

GC content: 39.41

Gene sequence:

>1317_bases
ATGAGTGACAATAACTACTCAAACAAAATTGCTATTGTTGGTATGGCTGTAAAATTTCCCGGAGCCAATGATCTTGATGA
ATATTGGGATTTATTGAAGTTTGGTAAAGAAGCAGTAACTAAATTTTCCGAAGAACAATTACATCGGTCAGGTATTTCAG
AGCAATTAATCGCTAATCCCAATTACAAACCCTACAGAGGGATATTAGATGACCTGGAGTCATTCGATACTGCTCCGTTT
GAGAATACCACCAAAAATTTTGAATTGTTGGGTGTGCAAGGCAAAGTGATGTCGCATTTAACTCATCGTGCTTTGAGTAC
GATGAGAAGTCAGGAACATCATTCAAATAACTACATAAAAAATACAGCTGTTTATATTGGCGCAAATAATCAACCAGCCG
CGCATTTCCTGGGAGCCGCCTACTACCAGTCGATTGATACTCAAAAAGCGATAGAAAGGTATTATTCAAAAGTCATCGCG
CCTTATATTTCTTATCAATTTGGATTTCAAGGAAGCTCCATTGATTTATATACAGCTTGCTCATCTTCTTTAACTGCGGT
CATCCAAAGCTGTAGAGAGTTAAACAGTCATCAATGTGATATGGCAATTGCAGGTGCGTGTTTAATCGATTTACCTCAGG
AAGTTGGGTATCTTTATGAAGAGAATGGCTTATTTTCATCCGACGGCCATTGCCATTCATTTGATGCTTCAGCATGCGGC
ACTTTATACTCAAATGGTGCTGGGTTAGTTGTTTTAAAGCGACTTGAAGATGCCATTGCAGATCATGATCTAATCCATGC
TGTGATTATCGGATCCGCCATGAATAATGATGGTAATTCTTCAAAAGAAGGATTTATGGCACCAGGGGTTCATGGCCAAT
ATACTTGCTTGCAGTCTGCTTGGCGAAATGCAGAGGTTGAGCCAGATGATTTGGATTATATTGCAGCTCATGGATCCTCA
ACCAAACTGGGTGATGCAACAGAAATATACGCCCTGAAAAAAGCGTTCAAAGACGTTAAAAAGACATATAGATGTCCAAT
AAGTTCGGTGATAAGTAACCTGGGTCATACAGGCATCGTGTCCGGTATGGCTTCAATCATCAAAACAGTACTTATGCTCA
AAAATAAGGCTATTGTTCCTTCAATTAATTTTGAAACTCCTAATCCAGATTTTATGTTGGAAGACTCACCTATATACATC
GCCACTGAATTTCAAGATTTCCCTACAGTAAAAAAACACTATTTGGCTGGTGTTTCCAGCTATGGGGCAGGAGGCAGCAA
TACACATCTGGTTTTGCGCAGCTATGATGAAAACTAG

Upstream 100 bases:

>100_bases
GAATAACGTAGACTATGTTTCATGCTATGAACCCTTCCAATGTCGTCTGGTTAGTGTTTATTACATGTTGAGTGCGGCCA
AAGAAATGGAGCCATAAATT

Downstream 100 bases:

>100_bases
GGTTCTATTGATATTTTTTTTTTATTCCTGTTAAAAGTTTACCTGCTGCGGATTGCTCGTTAGAGAGCTATGTAGAATTT
AAGCAGCGAGGAACATGACA

Product: polyketide synthase, type I

Products: L-tryptophan [Cytoplasm]; L-tyrosine [Cytoplasm]; Proton [Cytoplasm]; L-phenylalanine [Cytoplasm] [C]

Alternate protein names: PKS [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MSDNNYSNKIAIVGMAVKFPGANDLDEYWDLLKFGKEAVTKFSEEQLHRSGISEQLIANPNYKPYRGILDDLESFDTAPF
ENTTKNFELLGVQGKVMSHLTHRALSTMRSQEHHSNNYIKNTAVYIGANNQPAAHFLGAAYYQSIDTQKAIERYYSKVIA
PYISYQFGFQGSSIDLYTACSSSLTAVIQSCRELNSHQCDMAIAGACLIDLPQEVGYLYEENGLFSSDGHCHSFDASACG
TLYSNGAGLVVLKRLEDAIADHDLIHAVIIGSAMNNDGNSSKEGFMAPGVHGQYTCLQSAWRNAEVEPDDLDYIAAHGSS
TKLGDATEIYALKKAFKDVKKTYRCPISSVISNLGHTGIVSGMASIIKTVLMLKNKAIVPSINFETPNPDFMLEDSPIYI
ATEFQDFPTVKKHYLAGVSSYGAGGSNTHLVLRSYDEN

Sequences:

>Translated_438_residues
MSDNNYSNKIAIVGMAVKFPGANDLDEYWDLLKFGKEAVTKFSEEQLHRSGISEQLIANPNYKPYRGILDDLESFDTAPF
ENTTKNFELLGVQGKVMSHLTHRALSTMRSQEHHSNNYIKNTAVYIGANNQPAAHFLGAAYYQSIDTQKAIERYYSKVIA
PYISYQFGFQGSSIDLYTACSSSLTAVIQSCRELNSHQCDMAIAGACLIDLPQEVGYLYEENGLFSSDGHCHSFDASACG
TLYSNGAGLVVLKRLEDAIADHDLIHAVIIGSAMNNDGNSSKEGFMAPGVHGQYTCLQSAWRNAEVEPDDLDYIAAHGSS
TKLGDATEIYALKKAFKDVKKTYRCPISSVISNLGHTGIVSGMASIIKTVLMLKNKAIVPSINFETPNPDFMLEDSPIYI
ATEFQDFPTVKKHYLAGVSSYGAGGSNTHLVLRSYDEN
>Mature_437_residues
SDNNYSNKIAIVGMAVKFPGANDLDEYWDLLKFGKEAVTKFSEEQLHRSGISEQLIANPNYKPYRGILDDLESFDTAPFE
NTTKNFELLGVQGKVMSHLTHRALSTMRSQEHHSNNYIKNTAVYIGANNQPAAHFLGAAYYQSIDTQKAIERYYSKVIAP
YISYQFGFQGSSIDLYTACSSSLTAVIQSCRELNSHQCDMAIAGACLIDLPQEVGYLYEENGLFSSDGHCHSFDASACGT
LYSNGAGLVVLKRLEDAIADHDLIHAVIIGSAMNNDGNSSKEGFMAPGVHGQYTCLQSAWRNAEVEPDDLDYIAAHGSST
KLGDATEIYALKKAFKDVKKTYRCPISSVISNLGHTGIVSGMASIIKTVLMLKNKAIVPSINFETPNPDFMLEDSPIYIA
TEFQDFPTVKKHYLAGVSSYGAGGSNTHLVLRSYDEN

Specific function: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism [H]

COG id: COG3321

COG function: function code Q; Polyketide synthase modules and related proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 5 acyl carrier domains [H]

Homologues:

Organism=Homo sapiens, GI41872631, Length=433, Percent_Identity=28.175519630485, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI8923559, Length=289, Percent_Identity=26.9896193771626, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI223972627, Length=145, Percent_Identity=31.7241379310345, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788663, Length=440, Percent_Identity=24.3181818181818, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1787337, Length=284, Percent_Identity=26.056338028169, Blast_Score=86, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI212642053, Length=435, Percent_Identity=28.0459770114943, Blast_Score=158, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI17550940, Length=446, Percent_Identity=27.5784753363229, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI71983541, Length=293, Percent_Identity=25.938566552901, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71983535, Length=266, Percent_Identity=27.0676691729323, Blast_Score=81, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6320904, Length=292, Percent_Identity=24.6575342465753, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24581345, Length=431, Percent_Identity=27.3781902552204, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI19920632, Length=437, Percent_Identity=28.3752860411899, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI221330659, Length=437, Percent_Identity=28.3752860411899, Blast_Score=135, Evalue=8e-32,
Organism=Drosophila melanogaster, GI24644341, Length=462, Percent_Identity=24.025974025974, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: 24024 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 30,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR000794
- InterPro:   IPR001242
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR016040
- InterPro:   IPR006163
- InterPro:   IPR020842
- InterPro:   IPR020841
- InterPro:   IPR020807
- InterPro:   IPR013968
- InterPro:   IPR020806
- InterPro:   IPR006162
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C; PF08659 KR; PF00550 PP-binding [H]

EC number: 2.3.1.41 [C]

Molecular weight: Translated: 48071; Mature: 47940

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00606 B_KETOACYL_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNNYSNKIAIVGMAVKFPGANDLDEYWDLLKFGKEAVTKFSEEQLHRSGISEQLIANP
CCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCC
NYKPYRGILDDLESFDTAPFENTTKNFELLGVQGKVMSHLTHRALSTMRSQEHHSNNYIK
CCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEE
NTAVYIGANNQPAAHFLGAAYYQSIDTQKAIERYYSKVIAPYISYQFGFQGSSIDLYTAC
EEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEHHH
SSSLTAVIQSCRELNSHQCDMAIAGACLIDLPQEVGYLYEENGLFSSDGHCHSFDASACG
HHHHHHHHHHHHHHCCCCCCHHHHHHHHEECHHHHCCEEECCCCCCCCCCCCCCCCHHHH
TLYSNGAGLVVLKRLEDAIADHDLIHAVIIGSAMNNDGNSSKEGFMAPGVHGQYTCLQSA
HHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHH
WRNAEVEPDDLDYIAAHGSSTKLGDATEIYALKKAFKDVKKTYRCPISSVISNLGHTGIV
HCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHH
SGMASIIKTVLMLKNKAIVPSINFETPNPDFMLEDSPIYIATEFQDFPTVKKHYLAGVSS
HHHHHHHHHHHHHCCCCCCCEECCCCCCCCEEECCCCEEEEECCCCCCHHHHHHHHHHHH
YGAGGSNTHLVLRSYDEN
CCCCCCCCEEEEEECCCC
>Mature Secondary Structure 
SDNNYSNKIAIVGMAVKFPGANDLDEYWDLLKFGKEAVTKFSEEQLHRSGISEQLIANP
CCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCC
NYKPYRGILDDLESFDTAPFENTTKNFELLGVQGKVMSHLTHRALSTMRSQEHHSNNYIK
CCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEE
NTAVYIGANNQPAAHFLGAAYYQSIDTQKAIERYYSKVIAPYISYQFGFQGSSIDLYTAC
EEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEHHH
SSSLTAVIQSCRELNSHQCDMAIAGACLIDLPQEVGYLYEENGLFSSDGHCHSFDASACG
HHHHHHHHHHHHHHCCCCCCHHHHHHHHEECHHHHCCEEECCCCCCCCCCCCCCCCHHHH
TLYSNGAGLVVLKRLEDAIADHDLIHAVIIGSAMNNDGNSSKEGFMAPGVHGQYTCLQSA
HHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHH
WRNAEVEPDDLDYIAAHGSSTKLGDATEIYALKKAFKDVKKTYRCPISSVISNLGHTGIV
HCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHH
SGMASIIKTVLMLKNKAIVPSINFETPNPDFMLEDSPIYIATEFQDFPTVKKHYLAGVSS
HHHHHHHHHHHHHCCCCCCCEECCCCCCCCEEECCCCEEEEECCCCCCHHHHHHHHHHHH
YGAGGSNTHLVLRSYDEN
CCCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 2-Mercaptoethanol; DTT; EDTA [C]

Metal ions: Phosphate [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.04 {acetyl-ACP}} 0.031 {acetyl-ACP}} 0.138 {cis-9-hexadecenoyl-ACP}} 0.017 {cis-9-hexadecenoyl-ACP}} 0.012 {cis-3-decenoyl-ACP}} [C]

Substrates: L-tryptophan [Periplasm]; L-tyrosine [Periplasm]; Proton [Periplasm]; L-phenylalanine [Periplasm] [C]

Specific reaction: acyl-ACP + malonyl-ACP = beta-keto-enoyl-ACP + [acyl carrier protein] + CO2 acetyl-ACP + malonyl-ACP = [acyl carrier protein] + acetoacetyl-ACP + CO2 acyl-ACP + malonyl-ACP = [acyl carrier protein] + beta-ketoacyl-ACP + CO2 malonyl-ACP = acetyl-ACP + CO2

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl-CoA; Acyl-CoA; Iodoacetamide; N-Ethyl maleimide acyl -ACP-derivatives protect, malonyl -ACP-decarboxyl ation, [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704258; 9384377; 10568751 [H]