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The map label for this gene is mycC [H]

Identifier: 52842157

GI number: 52842157

Start: 2167602

End: 2169122

Strand: Reverse

Name: mycC [H]

Synonym: lpg1940

Alternate gene names: 52842157

Gene position: 2169122-2167602 (Counterclockwise)

Preceding gene: 52842158

Following gene: 52842156

Centisome position: 63.84

GC content: 37.41

Gene sequence:

>1521_bases
ATGAGATCTCCTGATCAGGACACGACAATTTGCTCCTTATTTGAGGAGAAGGTAAAGCAATATCCAGAAAATACAGCGGT
TTATCTGGAAGATCGTCAAATTTCGTATGATGCCTTTAATAAACAAGTCAACCAATACGCACGGTATCTGCAAAGAAAAG
GACTGCCATTAAATGGAATTGTGGGCATTCAGCTCACGCGTTCTTTTGAAATGCTGGTGGCAATTTTTGCGGTATTAAAG
GCAGGAGGTGCTTATTTACCGATAGATCCCATGGCTCCGGTCATAAGAAATCAAACCATCCTTGAAGACAGCCAAATCAA
ATTTCTAATTGTTAGTGATAATTCATTATTGACCCAAAAAGCTGGGTTGCAAGTATTAAGCATTCATGCCAATGTGAGCG
ATGAAGATTGCCATAATTTAAACCTGCCTCGAGAAAAACAGGATTTAGCTTATGTGATGTATACTTCAGGCACTACTGGC
AAACCGAAAGGGGTCATGATTTCACATCAGGCTCTGGTAAATCGAATTCTATGGATGCAAAACACATTTCCATTGCAAAC
TCATGATGTAGTGTTTCATAAAACACACATTTGCTTTGACATCTCAGTTTGGGAAACCTGTTGGTGGTCTATTGCGGGGG
CCGGAGTAGTACTATTACCGCCAGGAAAAGAACATGATATCAAGCTATTTATTCAAATGATTGAGCAATATCAAATAAAC
GTTATTCATTTTGTACCCTCCGTATTACGAATATTTTTAAGTTACATCGAACAGGATTTTTCGATAAATCGCTTAAGCTC
TTTAAAGTATGTCTTTTCAAGTGGAGAGGCACTGGATGCCAAAAGTGTTAATCTATTTAATAAGTTACTTCAAGATCAAA
AAACAATATTAGTCAATTTATACGGACCCACAGAGGCTACAATAGATGTTTCCTGTTTTATTTGTGAAAAACATAAAGAT
TATCGCACTGTGCCAATTGGTAAGCCTATCCAAAATACTCAATTTTTTGTGTTAGATGAGCAGTTAAACTCAACCGGTTT
CGAACCTGGAGAGCTATATATTTCCGGAGTTGGATTGGCTCAGGGTTATTTAAATAATCCAGCGCTTACTCGGACATCGT
TTATAGACAATCCCTACTTGCCTGGAGAAAAAATGTATCGTACTGGAGATATAGTCCGTTGGAATGAAAATCATGAATTA
TTGTTTTTAGGTCGCAGGGATGATCAGGTCAAATTACATGGTATACGTATTGAATTAGGAGAAATACAACATCATTTATT
AGATCATCCCAACATCCAGGATGCCGTCGTTGTCTGTGAGAAAGTAGAGGGTCTTGATTATAGAATGGTCGTGTTCCTAA
TTGCTAATAATCAAACAAAGATTGGCAGCGCTGAACTTAAATCGTTTTTAAAAACCAGGCTTCCAGACTATATGGTACCA
GAGCATTATCTATGGCTAAGCGCATTCCCAATAAAAGAAAATGGAAAAATAGACAAAGAGAAATTAATGACTTTCATTTA
G

Upstream 100 bases:

>100_bases
AGAGATGCTAAGGTAATGGAGATCATTGAAGGAACTTCGCAAATGCACGAGATACTCATTTCGAAACTTTGCATCAATAA
ATAGAGGATATTATTAAACA

Downstream 100 bases:

>100_bases
CTTGGGCAACATCTGAGTAATAAAAATAATTGAGGAAAATGACTTATACTTAATTAAAAGCTGGTTGCAGGAATAACGTA
GACTATGTTTCATGCTATGA

Product: peptide synthetase

Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]

Alternate protein names: ATP-dependent serine adenylase; SerA; Serine activase; ATP-dependent asparagine adenylase 3; AsnA 3; Asparagine activase 3 [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MRSPDQDTTICSLFEEKVKQYPENTAVYLEDRQISYDAFNKQVNQYARYLQRKGLPLNGIVGIQLTRSFEMLVAIFAVLK
AGGAYLPIDPMAPVIRNQTILEDSQIKFLIVSDNSLLTQKAGLQVLSIHANVSDEDCHNLNLPREKQDLAYVMYTSGTTG
KPKGVMISHQALVNRILWMQNTFPLQTHDVVFHKTHICFDISVWETCWWSIAGAGVVLLPPGKEHDIKLFIQMIEQYQIN
VIHFVPSVLRIFLSYIEQDFSINRLSSLKYVFSSGEALDAKSVNLFNKLLQDQKTILVNLYGPTEATIDVSCFICEKHKD
YRTVPIGKPIQNTQFFVLDEQLNSTGFEPGELYISGVGLAQGYLNNPALTRTSFIDNPYLPGEKMYRTGDIVRWNENHEL
LFLGRRDDQVKLHGIRIELGEIQHHLLDHPNIQDAVVVCEKVEGLDYRMVVFLIANNQTKIGSAELKSFLKTRLPDYMVP
EHYLWLSAFPIKENGKIDKEKLMTFI

Sequences:

>Translated_506_residues
MRSPDQDTTICSLFEEKVKQYPENTAVYLEDRQISYDAFNKQVNQYARYLQRKGLPLNGIVGIQLTRSFEMLVAIFAVLK
AGGAYLPIDPMAPVIRNQTILEDSQIKFLIVSDNSLLTQKAGLQVLSIHANVSDEDCHNLNLPREKQDLAYVMYTSGTTG
KPKGVMISHQALVNRILWMQNTFPLQTHDVVFHKTHICFDISVWETCWWSIAGAGVVLLPPGKEHDIKLFIQMIEQYQIN
VIHFVPSVLRIFLSYIEQDFSINRLSSLKYVFSSGEALDAKSVNLFNKLLQDQKTILVNLYGPTEATIDVSCFICEKHKD
YRTVPIGKPIQNTQFFVLDEQLNSTGFEPGELYISGVGLAQGYLNNPALTRTSFIDNPYLPGEKMYRTGDIVRWNENHEL
LFLGRRDDQVKLHGIRIELGEIQHHLLDHPNIQDAVVVCEKVEGLDYRMVVFLIANNQTKIGSAELKSFLKTRLPDYMVP
EHYLWLSAFPIKENGKIDKEKLMTFI
>Mature_506_residues
MRSPDQDTTICSLFEEKVKQYPENTAVYLEDRQISYDAFNKQVNQYARYLQRKGLPLNGIVGIQLTRSFEMLVAIFAVLK
AGGAYLPIDPMAPVIRNQTILEDSQIKFLIVSDNSLLTQKAGLQVLSIHANVSDEDCHNLNLPREKQDLAYVMYTSGTTG
KPKGVMISHQALVNRILWMQNTFPLQTHDVVFHKTHICFDISVWETCWWSIAGAGVVLLPPGKEHDIKLFIQMIEQYQIN
VIHFVPSVLRIFLSYIEQDFSINRLSSLKYVFSSGEALDAKSVNLFNKLLQDQKTILVNLYGPTEATIDVSCFICEKHKD
YRTVPIGKPIQNTQFFVLDEQLNSTGFEPGELYISGVGLAQGYLNNPALTRTSFIDNPYLPGEKMYRTGDIVRWNENHEL
LFLGRRDDQVKLHGIRIELGEIQHHLLDHPNIQDAVVVCEKVEGLDYRMVVFLIANNQTKIGSAELKSFLKTRLPDYMVP
EHYLWLSAFPIKENGKIDKEKLMTFI

Specific function: This protein is a multifunctional enzyme, able to activate and polymerize the amino acids Ser and Asn as part of the synthesis of mycosubtilin. The Ser residue is further epimerized to the D-isomer form. The activation sites for these amino acids consist

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 acyl carrier domains [H]

Homologues:

Organism=Homo sapiens, GI45580730, Length=547, Percent_Identity=23.5831809872029, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI187761345, Length=479, Percent_Identity=23.3820459290188, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI187761343, Length=479, Percent_Identity=23.3820459290188, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI156151445, Length=549, Percent_Identity=21.4936247723133, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI42544132, Length=542, Percent_Identity=23.2472324723247, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI28416953, Length=557, Percent_Identity=20.6463195691203, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI115511026, Length=428, Percent_Identity=25.7009345794392, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI38505220, Length=434, Percent_Identity=23.2718894009217, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI157311624, Length=481, Percent_Identity=22.4532224532225, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI157311622, Length=481, Percent_Identity=22.4532224532225, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI58082049, Length=481, Percent_Identity=22.2453222453222, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI1786801, Length=510, Percent_Identity=37.843137254902, Blast_Score=321, Evalue=7e-89,
Organism=Escherichia coli, GI1786810, Length=515, Percent_Identity=23.3009708737864, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1788107, Length=550, Percent_Identity=21.2727272727273, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI145693145, Length=548, Percent_Identity=24.0875912408759, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790505, Length=522, Percent_Identity=21.6475095785441, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI221142682, Length=529, Percent_Identity=22.8733459357278, Blast_Score=83, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17556356, Length=515, Percent_Identity=26.9902912621359, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17550940, Length=491, Percent_Identity=23.4215885947047, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17560140, Length=352, Percent_Identity=24.7159090909091, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI32564420, Length=360, Percent_Identity=27.5, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI32564422, Length=360, Percent_Identity=27.5, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI32563687, Length=488, Percent_Identity=21.5163934426229, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17557194, Length=499, Percent_Identity=21.4428857715431, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI71985884, Length=368, Percent_Identity=22.8260869565217, Blast_Score=78, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17538037, Length=501, Percent_Identity=21.1576846307385, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71994690, Length=383, Percent_Identity=21.9321148825065, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71994703, Length=383, Percent_Identity=21.6710182767624, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71994694, Length=383, Percent_Identity=21.6710182767624, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17560308, Length=395, Percent_Identity=23.7974683544304, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319591, Length=594, Percent_Identity=25.0841750841751, Blast_Score=163, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6319699, Length=360, Percent_Identity=25.8333333333333, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6319264, Length=381, Percent_Identity=23.0971128608924, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648676, Length=509, Percent_Identity=31.041257367387, Blast_Score=220, Evalue=2e-57,
Organism=Drosophila melanogaster, GI18859661, Length=527, Percent_Identity=24.0986717267552, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24582852, Length=505, Percent_Identity=22.1782178217822, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24581924, Length=361, Percent_Identity=23.8227146814404, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21355181, Length=521, Percent_Identity=22.2648752399232, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24656500, Length=530, Percent_Identity=22.0754716981132, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21356947, Length=502, Percent_Identity=19.7211155378486, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR010060
- InterPro:   IPR006163
- InterPro:   IPR006162
- InterPro:   IPR001031 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00550 PP-binding; PF00975 Thioesterase [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 57806; Mature: 57806

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSPDQDTTICSLFEEKVKQYPENTAVYLEDRQISYDAFNKQVNQYARYLQRKGLPLNGI
CCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCE
VGIQLTRSFEMLVAIFAVLKAGGAYLPIDPMAPVIRNQTILEDSQIKFLIVSDNSLLTQK
EEEEEEHHHHHHHHHHHHHHCCCCEECCCCCCHHHCCCEEEECCCEEEEEEECCCEEEHH
AGLQVLSIHANVSDEDCHNLNLPREKQDLAYVMYTSGTTGKPKGVMISHQALVNRILWMQ
CCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHC
NTFPLQTHDVVFHKTHICFDISVWETCWWSIAGAGVVLLPPGKEHDIKLFIQMIEQYQIN
CCCCCCCCEEEEEEEEEEEEEEHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCC
VIHFVPSVLRIFLSYIEQDFSINRLSSLKYVFSSGEALDAKSVNLFNKLLQDQKTILVNL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEE
YGPTEATIDVSCFICEKHKDYRTVPIGKPIQNTQFFVLDEQLNSTGFEPGELYISGVGLA
ECCCCCEEEEEEEEEECCCCCEECCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEEEHH
QGYLNNPALTRTSFIDNPYLPGEKMYRTGDIVRWNENHELLFLGRRDDQVKLHGIRIELG
CCCCCCCCEEEEECCCCCCCCCCCEEECCCEEEECCCCCEEEEECCCCEEEEEEEEEEHH
EIQHHLLDHPNIQDAVVVCEKVEGLDYRMVVFLIANNQTKIGSAELKSFLKTRLPDYMVP
HHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCC
EHYLWLSAFPIKENGKIDKEKLMTFI
CCEEEEEEECCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MRSPDQDTTICSLFEEKVKQYPENTAVYLEDRQISYDAFNKQVNQYARYLQRKGLPLNGI
CCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCE
VGIQLTRSFEMLVAIFAVLKAGGAYLPIDPMAPVIRNQTILEDSQIKFLIVSDNSLLTQK
EEEEEEHHHHHHHHHHHHHHCCCCEECCCCCCHHHCCCEEEECCCEEEEEEECCCEEEHH
AGLQVLSIHANVSDEDCHNLNLPREKQDLAYVMYTSGTTGKPKGVMISHQALVNRILWMQ
CCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHC
NTFPLQTHDVVFHKTHICFDISVWETCWWSIAGAGVVLLPPGKEHDIKLFIQMIEQYQIN
CCCCCCCCEEEEEEEEEEEEEEHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCC
VIHFVPSVLRIFLSYIEQDFSINRLSSLKYVFSSGEALDAKSVNLFNKLLQDQKTILVNL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEE
YGPTEATIDVSCFICEKHKDYRTVPIGKPIQNTQFFVLDEQLNSTGFEPGELYISGVGLA
ECCCCCEEEEEEEEEECCCCCEECCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEEEHH
QGYLNNPALTRTSFIDNPYLPGEKMYRTGDIVRWNENHELLFLGRRDDQVKLHGIRIELG
CCCCCCCCEEEEECCCCCCCCCCCEEECCCEEEECCCCCEEEEECCCCEEEEEEEEEEHH
EIQHHLLDHPNIQDAVVVCEKVEGLDYRMVVFLIANNQTKIGSAELKSFLKTRLPDYMVP
HHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCC
EHYLWLSAFPIKENGKIDKEKLMTFI
CCEEEEEEECCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Phosphopantetheine. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10557314 [H]