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The map label for this gene is pyrE

Identifier: 52841436

GI number: 52841436

Start: 1332772

End: 1333404

Strand: Reverse

Name: pyrE

Synonym: lpg1204

Alternate gene names: 52841436

Gene position: 1333404-1332772 (Counterclockwise)

Preceding gene: 52841440

Following gene: 52841433

Centisome position: 39.24

GC content: 39.02

Gene sequence:

>633_bases
ATGAATCATACCAAATCATCTTTTATAAAATTAGCTCTGGAGTGTCAAGTACTTAAATTTGGTGAATTTACTTTAAAATC
AGGACGAATAAGTCCCTATTTCTTTAACGCTGGTTTATTTTATCACGGTGATTCCTTACGTAAGTTAGGTCAATTTTATG
CAAAAACCCTTTTAGAACAAGAAGTTTCTTTTGAGCACTTGTTTGGACCAGCGTACAAAGGTATCCCACTCGCTACCGCA
ACTGCAGTGGCCTTGGCTGAGTTAGGCAGGGACATTACAGTGACTTTTAATCGTAAAGAAGTAAAAACTCATGGAGAAGG
CGGACAATTAATTGGTTCGCCGCTAACAGGAAGGACAGTGATCATTGACGATGTGATTACAGCGGGCACAGCATTCAGAG
AATCACAAACCCTGATTAAAGAGAATGGAGGGATTCTTCGTGGTGTCATTATCGCTCTGGACAGATGCGAACGAGGATTA
ACTGAGAAATCAACCCTGTCAGAAATCAGGGAGCAAGGAATTGAAGTCTATTCAATTATCAATTTGTTTGATCTAATAGA
ATTTCTGAAAAATGACAATCAATACGAACAGGTACAAAAACTGGAGTCCTATCAAGAGCGTTATGGCGCATAA

Upstream 100 bases:

>100_bases
ATCCTGCACTATGATTAGCATATCAGTTTTTTGTTGATTGTTAAGGATTTTTTTAAAATATGTGATTGTTTTCAATAAAA
TTTTGTATTAGGAAACCAGA

Downstream 100 bases:

>100_bases
TATTAGGATTACAGATTTTCCTTAAAAAAGAGTAAGACAATCATCACTCATTGTGTTTTCAGTTGCTGAGCTATCCATGA
AGATCAGCAACTTACACTGA

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MNHTKSSFIKLALECQVLKFGEFTLKSGRISPYFFNAGLFYHGDSLRKLGQFYAKTLLEQEVSFEHLFGPAYKGIPLATA
TAVALAELGRDITVTFNRKEVKTHGEGGQLIGSPLTGRTVIIDDVITAGTAFRESQTLIKENGGILRGVIIALDRCERGL
TEKSTLSEIREQGIEVYSIINLFDLIEFLKNDNQYEQVQKLESYQERYGA

Sequences:

>Translated_210_residues
MNHTKSSFIKLALECQVLKFGEFTLKSGRISPYFFNAGLFYHGDSLRKLGQFYAKTLLEQEVSFEHLFGPAYKGIPLATA
TAVALAELGRDITVTFNRKEVKTHGEGGQLIGSPLTGRTVIIDDVITAGTAFRESQTLIKENGGILRGVIIALDRCERGL
TEKSTLSEIREQGIEVYSIINLFDLIEFLKNDNQYEQVQKLESYQERYGA
>Mature_210_residues
MNHTKSSFIKLALECQVLKFGEFTLKSGRISPYFFNAGLFYHGDSLRKLGQFYAKTLLEQEVSFEHLFGPAYKGIPLATA
TAVALAELGRDITVTFNRKEVKTHGEGGQLIGSPLTGRTVIIDDVITAGTAFRESQTLIKENGGILRGVIIALDRCERGL
TEKSTLSEIREQGIEVYSIINLFDLIEFLKNDNQYEQVQKLESYQERYGA

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=185, Percent_Identity=28.1081081081081, Blast_Score=72, Evalue=4e-13,
Organism=Escherichia coli, GI1790073, Length=212, Percent_Identity=55.188679245283, Blast_Score=219, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI17508631, Length=192, Percent_Identity=25.5208333333333, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323530, Length=218, Percent_Identity=46.7889908256881, Blast_Score=181, Evalue=7e-47,
Organism=Saccharomyces cerevisiae, GI6323927, Length=215, Percent_Identity=43.7209302325581, Blast_Score=167, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 23492; Mature: 23492

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHTKSSFIKLALECQVLKFGEFTLKSGRISPYFFNAGLFYHGDSLRKLGQFYAKTLLEQ
CCCCHHHHHHHHHHHEEEECCCEEECCCCCCCEEEECCEEEECHHHHHHHHHHHHHHHHH
EVSFEHLFGPAYKGIPLATATAVALAELGRDITVTFNRKEVKTHGEGGQLIGSPLTGRTV
HHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEECHHHHEECCCCCCEECCCCCCCEE
IIDDVITAGTAFRESQTLIKENGGILRGVIIALDRCERGLTEKSTLSEIREQGIEVYSII
EEEHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHH
NLFDLIEFLKNDNQYEQVQKLESYQERYGA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNHTKSSFIKLALECQVLKFGEFTLKSGRISPYFFNAGLFYHGDSLRKLGQFYAKTLLEQ
CCCCHHHHHHHHHHHEEEECCCEEECCCCCCCEEEECCEEEECHHHHHHHHHHHHHHHHH
EVSFEHLFGPAYKGIPLATATAVALAELGRDITVTFNRKEVKTHGEGGQLIGSPLTGRTV
HHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEECHHHHEECCCCCCEECCCCCCCEE
IIDDVITAGTAFRESQTLIKENGGILRGVIIALDRCERGLTEKSTLSEIREQGIEVYSII
EEEHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHH
NLFDLIEFLKNDNQYEQVQKLESYQERYGA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA