Click here to switch to the map view.

The map label for this gene is pilS

Identifier: 52841407

GI number: 52841407

Start: 1296252

End: 1297838

Strand: Direct

Name: pilS

Synonym: lpg1173

Alternate gene names: 52841407

Gene position: 1296252-1297838 (Clockwise)

Preceding gene: 52841406

Following gene: 52841408

Centisome position: 38.15

GC content: 35.79

Gene sequence:

>1587_bases
ATGTCTGCTGCTCGCCAAATCAATAATCCGAAAATGAGACAATGGCGAATATTGTTGGTTTACAATATTTACAGAATAGT
AAGTATTTTTCTATTTTTAGGCATTTATTATTTCAGTTATATCAATAAATATTATCCCTTATTATTTCTCTGTATCGCTT
TTGCCTATTTAGTATTTGCTTTGATTTTTCTTTATTTTTGGCATAGAAAAATTTTAAGTTTTGATAAGCAGGTGTTTTTA
TCGGGTACGATTGATGTTATAGCCATTTCCAGCATGCTGGGTCTCATTGGTAATTTGGAATCCGGTTATGGCATATTATT
AAATGTGACTATTGCTGCTTTAAGCATTTTAGTTCCCGGGCGGTTGGCCATCTATTTTGCATCACTGGCCAGTTGTTTAT
TGCTATGTGGGAATGTTTTGCAATTTTTTATTTATAATCAAAAAGACTTAAGCACTTTTTTTTATAGTGGAATCTATGGG
GCTGGTTTTTTTGCAACGGCTATCACCGCCTGGTATTTATCCAATTGGGTCAGAATGTCTGAGAGTCTGGCCAGACACCG
TAGCTATGAGTTGGCAGGAATGCAAAGGCTCAATGAATATATTGTCGAACGATTGCATTCAGGCATTATTTATGTTGCAG
AGGGAAAGCGAATCCGGTTGATGAATACTGCTGCCCGTGAATTTTTTAATCTGAGTAAACATCATGCAATTCAAAAATTG
GACCAACTGTCTGATTCACTTGTATCCAAATTTGATGATTTTCTTTTAAAAACAACGAATAATGAACGTACAGCTCAAAC
TATTATTGAAGATCCTTATCTTAGGGTACATTTTTTTTCTACTTCTGTTGGACATAAGTCTGAAGTACTGATTATTCTGG
AGGACATGACTTATATTGCACAACAAGCACAACAACTCAAATTAGCGGCTTTAGGGCGTTTTTCGGCAAGTATTGCTCAT
GAATTACGTAATCCCTTGGGCGCGATTGCTCATGCTATTCAATTGTTTGGAGATAATGAGGATTTAAATGAAGAGAATAC
TCGTTTAAAACAATTAATCATGAACAATTGTGAGCGAATGAACAGGGTGATTAAAAATGTATTGCAACTTTCAAGGCGTC
AGAAATCTCAACCACAGGTTAATGAACTTGCTACATTTTTAGAACATTTTAAGCAGGATTTTACTCACCATAATCCCTGT
AACCTTACGATAAAATTACCTGCCAATAAGTCGTTATCGGTGGTCTTTGATAAAAGTCAATTGGAGCAGATTTTGGTTAT
ATTGTGTGAAAATTCCATTCAACATGGACGGGATCATGAAGGTAATGTGAATATAGTCATTGCTGCCAAATCTTCTTCGA
ATAAAATCACTTTGACGGTATGCGATTCTGGACCGGGTGTACCTCCAGAACACAGAGATAATATCTTTGAACCATTTTTT
ACCACCTTGCGAGGTGGTACTGGGATGGGGTTATTTATTGCCAGAGATTTGTGTGAAATTAATCAAGCCAGGCTAAATTT
GTTAAACTCAAATAAAGGAAGCTGTTTTGCGATCACTGTGAATCCATCGGATGAACTTTTAATATGA

Upstream 100 bases:

>100_bases
AGTGATCATTGGTGTGGGTCTTAGTTGATGTATTGTGCAAAATGCTCAAGTCTGGTTATATAATTAAAATACGCTGAATA
ATCTAATGGATTAGAAAAGG

Downstream 100 bases:

>100_bases
TCAAAAGTAAAGTGCTTGTTATAGATGACGAACCAGACATTAGAGAGTTATTAACTCTTACTTTATCACGCATGGGTTTA
ATTTGTGATGCGGTGTCTGA

Product: sensor protein PilS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MSAARQINNPKMRQWRILLVYNIYRIVSIFLFLGIYYFSYINKYYPLLFLCIAFAYLVFALIFLYFWHRKILSFDKQVFL
SGTIDVIAISSMLGLIGNLESGYGILLNVTIAALSILVPGRLAIYFASLASCLLLCGNVLQFFIYNQKDLSTFFYSGIYG
AGFFATAITAWYLSNWVRMSESLARHRSYELAGMQRLNEYIVERLHSGIIYVAEGKRIRLMNTAAREFFNLSKHHAIQKL
DQLSDSLVSKFDDFLLKTTNNERTAQTIIEDPYLRVHFFSTSVGHKSEVLIILEDMTYIAQQAQQLKLAALGRFSASIAH
ELRNPLGAIAHAIQLFGDNEDLNEENTRLKQLIMNNCERMNRVIKNVLQLSRRQKSQPQVNELATFLEHFKQDFTHHNPC
NLTIKLPANKSLSVVFDKSQLEQILVILCENSIQHGRDHEGNVNIVIAAKSSSNKITLTVCDSGPGVPPEHRDNIFEPFF
TTLRGGTGMGLFIARDLCEINQARLNLLNSNKGSCFAITVNPSDELLI

Sequences:

>Translated_528_residues
MSAARQINNPKMRQWRILLVYNIYRIVSIFLFLGIYYFSYINKYYPLLFLCIAFAYLVFALIFLYFWHRKILSFDKQVFL
SGTIDVIAISSMLGLIGNLESGYGILLNVTIAALSILVPGRLAIYFASLASCLLLCGNVLQFFIYNQKDLSTFFYSGIYG
AGFFATAITAWYLSNWVRMSESLARHRSYELAGMQRLNEYIVERLHSGIIYVAEGKRIRLMNTAAREFFNLSKHHAIQKL
DQLSDSLVSKFDDFLLKTTNNERTAQTIIEDPYLRVHFFSTSVGHKSEVLIILEDMTYIAQQAQQLKLAALGRFSASIAH
ELRNPLGAIAHAIQLFGDNEDLNEENTRLKQLIMNNCERMNRVIKNVLQLSRRQKSQPQVNELATFLEHFKQDFTHHNPC
NLTIKLPANKSLSVVFDKSQLEQILVILCENSIQHGRDHEGNVNIVIAAKSSSNKITLTVCDSGPGVPPEHRDNIFEPFF
TTLRGGTGMGLFIARDLCEINQARLNLLNSNKGSCFAITVNPSDELLI
>Mature_527_residues
SAARQINNPKMRQWRILLVYNIYRIVSIFLFLGIYYFSYINKYYPLLFLCIAFAYLVFALIFLYFWHRKILSFDKQVFLS
GTIDVIAISSMLGLIGNLESGYGILLNVTIAALSILVPGRLAIYFASLASCLLLCGNVLQFFIYNQKDLSTFFYSGIYGA
GFFATAITAWYLSNWVRMSESLARHRSYELAGMQRLNEYIVERLHSGIIYVAEGKRIRLMNTAAREFFNLSKHHAIQKLD
QLSDSLVSKFDDFLLKTTNNERTAQTIIEDPYLRVHFFSTSVGHKSEVLIILEDMTYIAQQAQQLKLAALGRFSASIAHE
LRNPLGAIAHAIQLFGDNEDLNEENTRLKQLIMNNCERMNRVIKNVLQLSRRQKSQPQVNELATFLEHFKQDFTHHNPCN
LTIKLPANKSLSVVFDKSQLEQILVILCENSIQHGRDHEGNVNIVIAAKSSSNKITLTVCDSGPGVPPEHRDNIFEPFFT
TLRGGTGMGLFIARDLCEINQARLNLLNSNKGSCFAITVNPSDELLI

Specific function: Member of the two-component regulatory system pilS/pilR involved in the regulation of the expression of type 4 fimbriae. PilS may function as a membrane-associated protein kinase that phosphorylates pilR in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=215, Percent_Identity=31.1627906976744, Blast_Score=106, Evalue=5e-24,
Organism=Escherichia coli, GI1788549, Length=348, Percent_Identity=25.2873563218391, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI87081816, Length=231, Percent_Identity=26.8398268398268, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1790300, Length=264, Percent_Identity=23.8636363636364, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1786912, Length=255, Percent_Identity=25.0980392156863, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1786600, Length=259, Percent_Identity=25.4826254826255, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI145693157, Length=357, Percent_Identity=23.5294117647059, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1789149, Length=247, Percent_Identity=25.5060728744939, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI87082128, Length=264, Percent_Identity=24.6212121212121, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 60032; Mature: 59901

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAARQINNPKMRQWRILLVYNIYRIVSIFLFLGIYYFSYINKYYPLLFLCIAFAYLVFA
CCCCCCCCCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIFLYFWHRKILSFDKQVFLSGTIDVIAISSMLGLIGNLESGYGILLNVTIAALSILVPG
HHHHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHCCC
RLAIYFASLASCLLLCGNVLQFFIYNQKDLSTFFYSGIYGAGFFATAITAWYLSNWVRMS
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESLARHRSYELAGMQRLNEYIVERLHSGIIYVAEGKRIRLMNTAAREFFNLSKHHAIQKL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEHHHHHHHHHCCHHHHHHHHH
DQLSDSLVSKFDDFLLKTTNNERTAQTIIEDPYLRVHFFSTSVGHKSEVLIILEDMTYIA
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEHHHHHHH
QQAQQLKLAALGRFSASIAHELRNPLGAIAHAIQLFGDNEDLNEENTRLKQLIMNNCERM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
NRVIKNVLQLSRRQKSQPQVNELATFLEHFKQDFTHHNPCNLTIKLPANKSLSVVFDKSQ
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEECHHH
LEQILVILCENSIQHGRDHEGNVNIVIAAKSSSNKITLTVCDSGPGVPPEHRDNIFEPFF
HHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCEEEEEEECCCCCCCCCCCCCHHHHHH
TTLRGGTGMGLFIARDLCEINQARLNLLNSNKGSCFAITVNPSDELLI
HHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure 
SAARQINNPKMRQWRILLVYNIYRIVSIFLFLGIYYFSYINKYYPLLFLCIAFAYLVFA
CCCCCCCCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIFLYFWHRKILSFDKQVFLSGTIDVIAISSMLGLIGNLESGYGILLNVTIAALSILVPG
HHHHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHCCC
RLAIYFASLASCLLLCGNVLQFFIYNQKDLSTFFYSGIYGAGFFATAITAWYLSNWVRMS
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESLARHRSYELAGMQRLNEYIVERLHSGIIYVAEGKRIRLMNTAAREFFNLSKHHAIQKL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEHHHHHHHHHCCHHHHHHHHH
DQLSDSLVSKFDDFLLKTTNNERTAQTIIEDPYLRVHFFSTSVGHKSEVLIILEDMTYIA
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEHHHHHHH
QQAQQLKLAALGRFSASIAHELRNPLGAIAHAIQLFGDNEDLNEENTRLKQLIMNNCERM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
NRVIKNVLQLSRRQKSQPQVNELATFLEHFKQDFTHHNPCNLTIKLPANKSLSVVFDKSQ
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEECHHH
LEQILVILCENSIQHGRDHEGNVNIVIAAKSSSNKITLTVCDSGPGVPPEHRDNIFEPFF
HHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCEEEEEEECCCCCCCCCCCCCHHHHHH
TTLRGGTGMGLFIARDLCEINQARLNLLNSNKGSCFAITVNPSDELLI
HHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8097014; 10984043 [H]