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The map label for this gene is pilR

Identifier: 52841408

GI number: 52841408

Start: 1297835

End: 1299163

Strand: Direct

Name: pilR

Synonym: lpg1174

Alternate gene names: 52841408

Gene position: 1297835-1299163 (Clockwise)

Preceding gene: 52841407

Following gene: 52841411

Centisome position: 38.2

GC content: 40.18

Gene sequence:

>1329_bases
ATGATCAAAAGTAAAGTGCTTGTTATAGATGACGAACCAGACATTAGAGAGTTATTAACTCTTACTTTATCACGCATGGG
TTTAATTTGTGATGCGGTGTCTGATTTTAAGCAGGGCATAGAACATATAAAACAAAATTATTATTCTTTGGTTTTAACCG
ATATGCGTTTGCCTGACGGAGATGGTATAGAAATTGTTAAATTCATTCAGAAATACAAACCACAGTTGCCAGTCGCTGTC
ATTACTGCTTATGGAAATGTAGAAGGTGCGGTCAATACTTTAAAGGCTGGTGCATTTGATTATGTATCCAAGCCTGTTGA
CTTAAAGATGCTCAGAGAACTCGTTAAGACGGCATTATCCATGCAAAATCCGTCTACCGAGAGTCCTGATGAAGGTCTAT
TGGTTGGTGAGAGCCTGATAATGCAACAACTGCGTGAGAGCATTCATAAATTAGCTCGTAGTCAGGCACCGGTTTTCATT
CAAGGTGATTCGGGTGTTGGGAAAGAACTTGTTGCTCAGCTGATCCATGCTAATGGTCCCAGAAAAGATAAGCCATTTAT
TCCTGTAAATTGTGGTGCCATACCTGGTGAGTTGATGGAGTCAGAGTTTTTTGGCCATAAAAAAGGAAGTTTTACAGGAG
CGATTTCGGATAAAACAGGATTATTTGTTGCAGCTCATGGCGGCACTTTGTTTCTTGATGAAATCGCCGAACTTCCTCTC
GCCATGCAGGTTAAATTACTACGCGCAATTCAGGAGAAGGCAATTAAACCAATAGGTGAGTTATATGAAATTCCGGTCGA
TGTTCGCATTCTAAGTGCGAGTCATAAAAATTTGATTGAGGAAATCAATGCAGGGAGGTTTCGTCAAGATTTGTATTATC
GAATTAATGTGATTGAGCTGCGTGTTCCAACTTTAATGGAAAGGCTTTCTGATATCCCTTGCCTGACAGAGGCGATATTA
AAAAAATTATCTAAAAATCAAAACAGAAATTTGGTTAAAATAAATCCCTCTTGTTTTGAAATATTGAGCCGTTATCATTT
CCCGGGAAATGTCAGAGAGTTGGAAAATATCCTTGAGCGTGCCATGGCTATGTGTGAAGGAGAGGTCATAGAGCCAAGCG
ATTTACATTTGCCCAGAACAACAATAACCCCAGCTGTTGCCGTACCCTCCACGTCAACTAACTTAAATGGTTACCTTCAG
GATCAGGAAAAAGAGTTAATTCTTGATGCGCTTGAGAAAACAAAATGGAACAGAACCGCTGCGGCACAACTACTGGGGGT
CAGCTTCCGAACTCTCCGTTATCGATTAAAAAAACTGGGACTTGATTAG

Upstream 100 bases:

>100_bases
GAGATTTGTGTGAAATTAATCAAGCCAGGCTAAATTTGTTAAACTCAAATAAAGGAAGCTGTTTTGCGATCACTGTGAAT
CCATCGGATGAACTTTTAAT

Downstream 100 bases:

>100_bases
ATGTTGCGCTTTGAAGGTTTTCAAAGGTTAAAATAGACCTCTTGCCTGGCCAGCTTCTTTCGTTTTATTGTCGCCCTGAA
AACGTTTCTGAGTTGCTCAT

Product: two component response regulator PilR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MIKSKVLVIDDEPDIRELLTLTLSRMGLICDAVSDFKQGIEHIKQNYYSLVLTDMRLPDGDGIEIVKFIQKYKPQLPVAV
ITAYGNVEGAVNTLKAGAFDYVSKPVDLKMLRELVKTALSMQNPSTESPDEGLLVGESLIMQQLRESIHKLARSQAPVFI
QGDSGVGKELVAQLIHANGPRKDKPFIPVNCGAIPGELMESEFFGHKKGSFTGAISDKTGLFVAAHGGTLFLDEIAELPL
AMQVKLLRAIQEKAIKPIGELYEIPVDVRILSASHKNLIEEINAGRFRQDLYYRINVIELRVPTLMERLSDIPCLTEAIL
KKLSKNQNRNLVKINPSCFEILSRYHFPGNVRELENILERAMAMCEGEVIEPSDLHLPRTTITPAVAVPSTSTNLNGYLQ
DQEKELILDALEKTKWNRTAAAQLLGVSFRTLRYRLKKLGLD

Sequences:

>Translated_442_residues
MIKSKVLVIDDEPDIRELLTLTLSRMGLICDAVSDFKQGIEHIKQNYYSLVLTDMRLPDGDGIEIVKFIQKYKPQLPVAV
ITAYGNVEGAVNTLKAGAFDYVSKPVDLKMLRELVKTALSMQNPSTESPDEGLLVGESLIMQQLRESIHKLARSQAPVFI
QGDSGVGKELVAQLIHANGPRKDKPFIPVNCGAIPGELMESEFFGHKKGSFTGAISDKTGLFVAAHGGTLFLDEIAELPL
AMQVKLLRAIQEKAIKPIGELYEIPVDVRILSASHKNLIEEINAGRFRQDLYYRINVIELRVPTLMERLSDIPCLTEAIL
KKLSKNQNRNLVKINPSCFEILSRYHFPGNVRELENILERAMAMCEGEVIEPSDLHLPRTTITPAVAVPSTSTNLNGYLQ
DQEKELILDALEKTKWNRTAAAQLLGVSFRTLRYRLKKLGLD
>Mature_442_residues
MIKSKVLVIDDEPDIRELLTLTLSRMGLICDAVSDFKQGIEHIKQNYYSLVLTDMRLPDGDGIEIVKFIQKYKPQLPVAV
ITAYGNVEGAVNTLKAGAFDYVSKPVDLKMLRELVKTALSMQNPSTESPDEGLLVGESLIMQQLRESIHKLARSQAPVFI
QGDSGVGKELVAQLIHANGPRKDKPFIPVNCGAIPGELMESEFFGHKKGSFTGAISDKTGLFVAAHGGTLFLDEIAELPL
AMQVKLLRAIQEKAIKPIGELYEIPVDVRILSASHKNLIEEINAGRFRQDLYYRINVIELRVPTLMERLSDIPCLTEAIL
KKLSKNQNRNLVKINPSCFEILSRYHFPGNVRELENILERAMAMCEGEVIEPSDLHLPRTTITPAVAVPSTSTNLNGYLQ
DQEKELILDALEKTKWNRTAAAQLLGVSFRTLRYRLKKLGLD

Specific function: Member of the two-component regulatory system pilS/pilR that regulates the expression of type 4 fimbriae. When activated by pilS it acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=439, Percent_Identity=42.1412300683371, Blast_Score=343, Evalue=1e-95,
Organism=Escherichia coli, GI1788550, Length=453, Percent_Identity=39.0728476821192, Blast_Score=335, Evalue=2e-93,
Organism=Escherichia coli, GI1788905, Length=433, Percent_Identity=37.8752886836028, Blast_Score=302, Evalue=3e-83,
Organism=Escherichia coli, GI1790299, Length=475, Percent_Identity=35.1578947368421, Blast_Score=292, Evalue=3e-80,
Organism=Escherichia coli, GI1789233, Length=336, Percent_Identity=41.3690476190476, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI1786524, Length=364, Percent_Identity=38.7362637362637, Blast_Score=228, Evalue=8e-61,
Organism=Escherichia coli, GI87082117, Length=323, Percent_Identity=38.390092879257, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI87082152, Length=337, Percent_Identity=40.0593471810089, Blast_Score=225, Evalue=3e-60,
Organism=Escherichia coli, GI1789087, Length=316, Percent_Identity=38.2911392405063, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1787583, Length=334, Percent_Identity=35.6287425149701, Blast_Score=201, Evalue=8e-53,
Organism=Escherichia coli, GI87081872, Length=315, Percent_Identity=35.2380952380952, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI87081858, Length=305, Percent_Identity=32.4590163934426, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1789828, Length=226, Percent_Identity=34.5132743362832, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1790552, Length=132, Percent_Identity=31.8181818181818, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1786599, Length=125, Percent_Identity=29.6, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082012, Length=115, Percent_Identity=35.6521739130435, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1786911, Length=127, Percent_Identity=30.7086614173228, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786784, Length=115, Percent_Identity=32.1739130434783, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49284; Mature: 49284

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKSKVLVIDDEPDIRELLTLTLSRMGLICDAVSDFKQGIEHIKQNYYSLVLTDMRLPDG
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCC
DGIEIVKFIQKYKPQLPVAVITAYGNVEGAVNTLKAGAFDYVSKPVDLKMLRELVKTALS
CCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHH
MQNPSTESPDEGLLVGESLIMQQLRESIHKLARSQAPVFIQGDSGVGKELVAQLIHANGP
CCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCC
RKDKPFIPVNCGAIPGELMESEFFGHKKGSFTGAISDKTGLFVAAHGGTLFLDEIAELPL
CCCCCEEEEECCCCCHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCEEEHHHHHHCCH
AMQVKLLRAIQEKAIKPIGELYEIPVDVRILSASHKNLIEEINAGRFRQDLYYRINVIEL
HHHHHHHHHHHHHHHCHHHHHHHCCCCEEEECCHHHHHHHHHCCCHHHHHHEEEEEEEEE
RVPTLMERLSDIPCLTEAILKKLSKNQNRNLVKINPSCFEILSRYHFPGNVRELENILER
EHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHCCCCCHHHHHHHHHH
AMAMCEGEVIEPSDLHLPRTTITPAVAVPSTSTNLNGYLQDQEKELILDALEKTKWNRTA
HHHHHCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCEECCHHHHHHHHHHHHHCCCHHH
AAQLLGVSFRTLRYRLKKLGLD
HHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKSKVLVIDDEPDIRELLTLTLSRMGLICDAVSDFKQGIEHIKQNYYSLVLTDMRLPDG
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCC
DGIEIVKFIQKYKPQLPVAVITAYGNVEGAVNTLKAGAFDYVSKPVDLKMLRELVKTALS
CCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHH
MQNPSTESPDEGLLVGESLIMQQLRESIHKLARSQAPVFIQGDSGVGKELVAQLIHANGP
CCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCC
RKDKPFIPVNCGAIPGELMESEFFGHKKGSFTGAISDKTGLFVAAHGGTLFLDEIAELPL
CCCCCEEEEECCCCCHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCEEEHHHHHHCCH
AMQVKLLRAIQEKAIKPIGELYEIPVDVRILSASHKNLIEEINAGRFRQDLYYRINVIEL
HHHHHHHHHHHHHHHCHHHHHHHCCCCEEEECCHHHHHHHHHCCCHHHHHHEEEEEEEEE
RVPTLMERLSDIPCLTEAILKKLSKNQNRNLVKINPSCFEILSRYHFPGNVRELENILER
EHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHCCCCCHHHHHHHHHH
AMAMCEGEVIEPSDLHLPRTTITPAVAVPSTSTNLNGYLQDQEKELILDALEKTKWNRTA
HHHHHCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCEECCHHHHHHHHHHHHHCCCHHH
AAQLLGVSFRTLRYRLKKLGLD
HHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1317379; 8097014; 7911557; 10984043 [H]