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The map label for this gene is pgk

Identifier: 52840392

GI number: 52840392

Start: 164776

End: 165966

Strand: Reverse

Name: pgk

Synonym: lpg0137

Alternate gene names: 52840392

Gene position: 165966-164776 (Counterclockwise)

Preceding gene: 52840393

Following gene: 52840391

Centisome position: 4.88

GC content: 45.0

Gene sequence:

>1191_bases
ATGAACCTGATTAAAATGAGCGACATTGATCTGTCTGGCAAAAGAGTTCTGATTCGTGAGGATTTAAATGTCCCGATCAA
AGACGGTATGATTACCAGTGATCAAAGATTGCAAGCGGCCCTGCCTACAATCAAATCCGCTTTGGACAGTGGCGCAGCAG
TAATCGTATTGTCTCATCTTGGGCGTCCGGAGGAAGGCAAATACGAAAAAAAATTCTCTTTGGAGCCTGTTGCTGATTAT
TTAAGGGAAAACCTGGAATATCCTGTTCGCTTCGTCAAAGATTACCTGAGCGGCGTGGGTGTAAACCCTGGGGAATTGGT
CGTATGCGAAAATGTCCGATTTAATCCCGGTGAAAAAGCCAATGACGAAGCTTTGGCAAAAAAACTGGCAAGCCTTTGTG
ATGTCTTTGTCATGGATGCTTTTGGCACGGCACACAGGGCACAAGCGTCTACCTATGGTGTCGCTCAATATGCTCCAGTT
GCCGTTGCTGGTCCTCTGCTAATCCGTGAACTTGAAGCGTTAAACCAAGTGCTTAAAGCGCCTAAAAAACCTATCGTCGC
TATTGTTGGAGGAGCCAAAGTCTCATCCAAGCTCAGTCTATTGAAACAATTGGTGGGTATGGTTGATGTTCTTATCCCTG
GCGGCGGTATTGCCAATACTTTTCTGAAAGCCCAAGGGTTTGAAATTGGTATTTCCCTTTACGAACCTGATTTATTGGAT
GAGGCTCGCCATATCCTTATTCTGGCCAAAGAGAAAGGTTGCCAAATTCCTCTACCCACCGACGTTGTAGTAGGCAAAAC
ATTTAGTGAAACCTGCCCTGCTTTCAACAAATCTCTGTCTAATGTGGCTGCGGACGATATGATCCTTGATATTGGCCCAG
AAACCATAAGAGATTATGTAGACCTCATCCATGACGCCAACACCATCATCTGGAATGGTCCGGTTGGTGTATTCGAATTC
CCGCAGTTTGCTTATGGAACTAGAGCCATTGCTATAGCGATTGCCGAGAGTGATGCCTTTTCTATTGCTGGTGGTGGTGA
TACCTTGGCTGCTGTTGATCTTTATGATTTGAATCAACAAATTTCTTATATTTCCACAGGAGGTGGGGCATTTCTCGAAT
GTCTGGAAGGAAAAACCTTGCCTGCTGTTGCTATTTTGCAGGAGCGCGCAAAACATGTTAAGACGAACTAA

Upstream 100 bases:

>100_bases
AATTTGGTTAAAGTAGTAGCCTGGTATGACAATGAATGGGGTTTCTCTAACCGCATGATTGATACCGCACACCAAATGAT
GAACTGCTAAAGGATAACCA

Downstream 100 bases:

>100_bases
GATTGTTGCCACTTTAGGCCCAGCCAGCAAGGAACCTGAAATTCTGCGATCCATGCTGGCTGCTGGAGTCAACGTAGTAC
GAATTAATTTTTCTCATGCC

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MNLIKMSDIDLSGKRVLIREDLNVPIKDGMITSDQRLQAALPTIKSALDSGAAVIVLSHLGRPEEGKYEKKFSLEPVADY
LRENLEYPVRFVKDYLSGVGVNPGELVVCENVRFNPGEKANDEALAKKLASLCDVFVMDAFGTAHRAQASTYGVAQYAPV
AVAGPLLIRELEALNQVLKAPKKPIVAIVGGAKVSSKLSLLKQLVGMVDVLIPGGGIANTFLKAQGFEIGISLYEPDLLD
EARHILILAKEKGCQIPLPTDVVVGKTFSETCPAFNKSLSNVAADDMILDIGPETIRDYVDLIHDANTIIWNGPVGVFEF
PQFAYGTRAIAIAIAESDAFSIAGGGDTLAAVDLYDLNQQISYISTGGGAFLECLEGKTLPAVAILQERAKHVKTN

Sequences:

>Translated_396_residues
MNLIKMSDIDLSGKRVLIREDLNVPIKDGMITSDQRLQAALPTIKSALDSGAAVIVLSHLGRPEEGKYEKKFSLEPVADY
LRENLEYPVRFVKDYLSGVGVNPGELVVCENVRFNPGEKANDEALAKKLASLCDVFVMDAFGTAHRAQASTYGVAQYAPV
AVAGPLLIRELEALNQVLKAPKKPIVAIVGGAKVSSKLSLLKQLVGMVDVLIPGGGIANTFLKAQGFEIGISLYEPDLLD
EARHILILAKEKGCQIPLPTDVVVGKTFSETCPAFNKSLSNVAADDMILDIGPETIRDYVDLIHDANTIIWNGPVGVFEF
PQFAYGTRAIAIAIAESDAFSIAGGGDTLAAVDLYDLNQQISYISTGGGAFLECLEGKTLPAVAILQERAKHVKTN
>Mature_396_residues
MNLIKMSDIDLSGKRVLIREDLNVPIKDGMITSDQRLQAALPTIKSALDSGAAVIVLSHLGRPEEGKYEKKFSLEPVADY
LRENLEYPVRFVKDYLSGVGVNPGELVVCENVRFNPGEKANDEALAKKLASLCDVFVMDAFGTAHRAQASTYGVAQYAPV
AVAGPLLIRELEALNQVLKAPKKPIVAIVGGAKVSSKLSLLKQLVGMVDVLIPGGGIANTFLKAQGFEIGISLYEPDLLD
EARHILILAKEKGCQIPLPTDVVVGKTFSETCPAFNKSLSNVAADDMILDIGPETIRDYVDLIHDANTIIWNGPVGVFEF
PQFAYGTRAIAIAIAESDAFSIAGGGDTLAAVDLYDLNQQISYISTGGGAFLECLEGKTLPAVAILQERAKHVKTN

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=413, Percent_Identity=39.225181598063, Blast_Score=275, Evalue=6e-74,
Organism=Homo sapiens, GI31543397, Length=411, Percent_Identity=38.6861313868613, Blast_Score=270, Evalue=2e-72,
Organism=Escherichia coli, GI1789294, Length=391, Percent_Identity=62.6598465473146, Blast_Score=499, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17508823, Length=414, Percent_Identity=42.2705314009662, Blast_Score=292, Evalue=2e-79,
Organism=Saccharomyces cerevisiae, GI6319857, Length=414, Percent_Identity=40.8212560386473, Blast_Score=261, Evalue=2e-70,
Organism=Drosophila melanogaster, GI17136394, Length=416, Percent_Identity=41.8269230769231, Blast_Score=265, Evalue=5e-71,
Organism=Drosophila melanogaster, GI24581203, Length=413, Percent_Identity=39.9515738498789, Blast_Score=249, Evalue=2e-66,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 42480; Mature: 42480

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLIKMSDIDLSGKRVLIREDLNVPIKDGMITSDQRLQAALPTIKSALDSGAAVIVLSHL
CCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECC
GRPEEGKYEKKFSLEPVADYLRENLEYPVRFVKDYLSGVGVNPGELVVCENVRFNPGEKA
CCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCC
NDEALAKKLASLCDVFVMDAFGTAHRAQASTYGVAQYAPVAVAGPLLIRELEALNQVLKA
CHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHC
PKKPIVAIVGGAKVSSKLSLLKQLVGMVDVLIPGGGIANTFLKAQGFEIGISLYEPDLLD
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEEEECCCHHH
EARHILILAKEKGCQIPLPTDVVVGKTFSETCPAFNKSLSNVAADDMILDIGPETIRDYV
CCCEEEEEECCCCCCCCCCCCEEECCCHHHHCCHHHHHHHHHHHCCEEEECCHHHHHHHH
DLIHDANTIIWNGPVGVFEFPQFAYGTRAIAIAIAESDAFSIAGGGDTLAAVDLYDLNQQ
HHHHCCCEEEECCCCCCCCCCCHHCCCEEEEEEEECCCCEEEECCCCCEEEEEEECCCCC
ISYISTGGGAFLECLEGKTLPAVAILQERAKHVKTN
EEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNLIKMSDIDLSGKRVLIREDLNVPIKDGMITSDQRLQAALPTIKSALDSGAAVIVLSHL
CCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECC
GRPEEGKYEKKFSLEPVADYLRENLEYPVRFVKDYLSGVGVNPGELVVCENVRFNPGEKA
CCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCC
NDEALAKKLASLCDVFVMDAFGTAHRAQASTYGVAQYAPVAVAGPLLIRELEALNQVLKA
CHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHC
PKKPIVAIVGGAKVSSKLSLLKQLVGMVDVLIPGGGIANTFLKAQGFEIGISLYEPDLLD
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEEEECCCHHH
EARHILILAKEKGCQIPLPTDVVVGKTFSETCPAFNKSLSNVAADDMILDIGPETIRDYV
CCCEEEEEECCCCCCCCCCCCEEECCCHHHHCCHHHHHHHHHHHCCEEEECCHHHHHHHH
DLIHDANTIIWNGPVGVFEFPQFAYGTRAIAIAIAESDAFSIAGGGDTLAAVDLYDLNQQ
HHHHCCCEEEECCCCCCCCCCCHHCCCEEEEEEEECCCCEEEECCCCCEEEEEEECCCCC
ISYISTGGGAFLECLEGKTLPAVAILQERAKHVKTN
EEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA