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The map label for this gene is pykA

Identifier: 52840391

GI number: 52840391

Start: 163368

End: 164792

Strand: Reverse

Name: pykA

Synonym: lpg0136

Alternate gene names: 52840391

Gene position: 164792-163368 (Counterclockwise)

Preceding gene: 52840392

Following gene: 52840390

Centisome position: 4.85

GC content: 43.23

Gene sequence:

>1425_bases
ATGTTAAGACGAACTAAGATTGTTGCCACTTTAGGCCCAGCCAGCAAGGAACCTGAAATTCTGCGATCCATGCTGGCTGC
TGGAGTCAACGTAGTACGAATTAATTTTTCTCATGCCGATTCCTCCGCCCTGCAATTAATTGCACTGGTTCGTAAAATCG
CAGACGAATTAAACCACCCGGTTGCAGTCATGGCTGACTTACAAGGGCCTAAAATAAGAGTCGGCCGTTTTCAAAACAAA
TCCATCACTTTAATTGATGGACAAAATTTTACATTGGATTGTATGGCCCCAGATACTCTGGGAGACATAAATGGCGTATC
GGTCGCTTATCCTAATTTGGCCAATGAACTCAGTATTGGTGATCATCTTCTGATTAATGATGGGTTAATTGAATTGGAAG
TCATAGAGATTTCCGGGTCAAAAATTCATTGCAAGGTAGTGGAAGGTGGAGTACTCACTGATTTGAAAGGTTTGAATCGA
AAAGGTGGCGGGTTAGCAGCCAGAACTTTAACAGAAAAAGATAGAAATGATTTACGTACAGCAATTGAAGCAGAAGTGGA
CTACATCAGTTTATCTTTTGTAAAAGACGCAGAGGATATCCGGCAAGCAAGAGCGCTAATGAAAGACTATGGGGCCCAAA
TAACCCCTATTATCGCTAAAATTGAGCGCATGGAAGCTTTGGATCACTTAACTGATATTATTCGCGAAGCAGACGCCATC
ATGGTGGCCAGAGGAGATTTAGGCGTTGAAGTGGGTGCTGCTGAAGTTCCCGCCATTCAAAAACACATCATAGAACAAAC
GCGACTTTTAGATAAAGTGGTGATTACCGCAACACAAATGATGGAATCCATGATAAGCAATCCTCAGCCTACCCGTGCCG
AGGTTTCTGATGTAGCCAACGCGATTCTGGACGGAACTGATGCTGTGATGCTTTCGGCTGAAACAGCAAGCGGCCTTTTT
CCAGTCAAAGTCATTACCATGGTCAATAAAATATGTTTAAGCGCTGAGAAGCATGCCAGCTTTTTTTATCATAGTGATCC
GGAAACCTGTCATTATCAACGGGCTGACCAGGCAATCGCTATGGCTACCATGCATACCGCCAATCATTTCCCCATTCAGG
CCATTATCACACTGACCGAATCCGGAGACACGGCTTTATGGGTATCCCGACACCACAGCACCGTTCCTATTTTTGCTATA
TCAGCCAATAAGAGAACCATTGGTCGTTTAAGCCTTGTAAACAATGTCTTTCCTATCTACATTGATTTTCATCAATTCAA
CCCCGAAGGATTGAATCAACAAATTTTACATGAATTAATCAAATCAGGGCACCTTGAAAAGAGAGGCTATGTGTTATTAA
CTCGCGGAACCCAAATTGGAATGCCTGGCGGCACCAACTGCATGGAAATTATACCTGTTGTCTGA

Upstream 100 bases:

>100_bases
CAACAAATTTCTTATATTTCCACAGGAGGTGGGGCATTTCTCGAATGTCTGGAAGGAAAAACCTTGCCTGCTGTTGCTAT
TTTGCAGGAGCGCGCAAAAC

Downstream 100 bases:

>100_bases
GTTCAAGATATCCTGTTAATTAAAGCAAGGTTCTTTCATAAAAGACCAAAATGATTATTTTAATGACACTTTAATCAAAT
TATCACCTCTATGCTACTAT

Product: pyruvate kinase II

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MLRRTKIVATLGPASKEPEILRSMLAAGVNVVRINFSHADSSALQLIALVRKIADELNHPVAVMADLQGPKIRVGRFQNK
SITLIDGQNFTLDCMAPDTLGDINGVSVAYPNLANELSIGDHLLINDGLIELEVIEISGSKIHCKVVEGGVLTDLKGLNR
KGGGLAARTLTEKDRNDLRTAIEAEVDYISLSFVKDAEDIRQARALMKDYGAQITPIIAKIERMEALDHLTDIIREADAI
MVARGDLGVEVGAAEVPAIQKHIIEQTRLLDKVVITATQMMESMISNPQPTRAEVSDVANAILDGTDAVMLSAETASGLF
PVKVITMVNKICLSAEKHASFFYHSDPETCHYQRADQAIAMATMHTANHFPIQAIITLTESGDTALWVSRHHSTVPIFAI
SANKRTIGRLSLVNNVFPIYIDFHQFNPEGLNQQILHELIKSGHLEKRGYVLLTRGTQIGMPGGTNCMEIIPVV

Sequences:

>Translated_474_residues
MLRRTKIVATLGPASKEPEILRSMLAAGVNVVRINFSHADSSALQLIALVRKIADELNHPVAVMADLQGPKIRVGRFQNK
SITLIDGQNFTLDCMAPDTLGDINGVSVAYPNLANELSIGDHLLINDGLIELEVIEISGSKIHCKVVEGGVLTDLKGLNR
KGGGLAARTLTEKDRNDLRTAIEAEVDYISLSFVKDAEDIRQARALMKDYGAQITPIIAKIERMEALDHLTDIIREADAI
MVARGDLGVEVGAAEVPAIQKHIIEQTRLLDKVVITATQMMESMISNPQPTRAEVSDVANAILDGTDAVMLSAETASGLF
PVKVITMVNKICLSAEKHASFFYHSDPETCHYQRADQAIAMATMHTANHFPIQAIITLTESGDTALWVSRHHSTVPIFAI
SANKRTIGRLSLVNNVFPIYIDFHQFNPEGLNQQILHELIKSGHLEKRGYVLLTRGTQIGMPGGTNCMEIIPVV
>Mature_474_residues
MLRRTKIVATLGPASKEPEILRSMLAAGVNVVRINFSHADSSALQLIALVRKIADELNHPVAVMADLQGPKIRVGRFQNK
SITLIDGQNFTLDCMAPDTLGDINGVSVAYPNLANELSIGDHLLINDGLIELEVIEISGSKIHCKVVEGGVLTDLKGLNR
KGGGLAARTLTEKDRNDLRTAIEAEVDYISLSFVKDAEDIRQARALMKDYGAQITPIIAKIERMEALDHLTDIIREADAI
MVARGDLGVEVGAAEVPAIQKHIIEQTRLLDKVVITATQMMESMISNPQPTRAEVSDVANAILDGTDAVMLSAETASGLF
PVKVITMVNKICLSAEKHASFFYHSDPETCHYQRADQAIAMATMHTANHFPIQAIITLTESGDTALWVSRHHSTVPIFAI
SANKRTIGRLSLVNNVFPIYIDFHQFNPEGLNQQILHELIKSGHLEKRGYVLLTRGTQIGMPGGTNCMEIIPVV

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI32967597, Length=496, Percent_Identity=36.2903225806452, Blast_Score=260, Evalue=2e-69,
Organism=Homo sapiens, GI10835121, Length=496, Percent_Identity=36.2903225806452, Blast_Score=260, Evalue=2e-69,
Organism=Homo sapiens, GI33286422, Length=491, Percent_Identity=34.2158859470468, Blast_Score=257, Evalue=2e-68,
Organism=Homo sapiens, GI33286420, Length=491, Percent_Identity=34.2158859470468, Blast_Score=257, Evalue=2e-68,
Organism=Homo sapiens, GI33286418, Length=499, Percent_Identity=33.8677354709419, Blast_Score=249, Evalue=5e-66,
Organism=Homo sapiens, GI310128732, Length=289, Percent_Identity=34.9480968858132, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI310128730, Length=289, Percent_Identity=34.9480968858132, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=32.0675105485232, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=32.0675105485232, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI310128738, Length=216, Percent_Identity=31.4814814814815, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1788160, Length=477, Percent_Identity=45.2830188679245, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1787965, Length=439, Percent_Identity=37.5854214123007, Blast_Score=292, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI17544584, Length=427, Percent_Identity=37.4707259953162, Blast_Score=261, Evalue=4e-70,
Organism=Caenorhabditis elegans, GI71984413, Length=487, Percent_Identity=36.1396303901437, Blast_Score=239, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI71984406, Length=487, Percent_Identity=36.1396303901437, Blast_Score=239, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI17506831, Length=487, Percent_Identity=36.5503080082135, Blast_Score=239, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI17506829, Length=487, Percent_Identity=36.1396303901437, Blast_Score=239, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6319279, Length=430, Percent_Identity=35.1162790697674, Blast_Score=243, Evalue=5e-65,
Organism=Saccharomyces cerevisiae, GI6324923, Length=476, Percent_Identity=32.7731092436975, Blast_Score=229, Evalue=9e-61,
Organism=Drosophila melanogaster, GI28571814, Length=434, Percent_Identity=36.1751152073733, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI24648964, Length=434, Percent_Identity=36.1751152073733, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI24648966, Length=414, Percent_Identity=36.4734299516908, Blast_Score=225, Evalue=4e-59,
Organism=Drosophila melanogaster, GI24581235, Length=429, Percent_Identity=33.5664335664336, Blast_Score=214, Evalue=8e-56,
Organism=Drosophila melanogaster, GI24646914, Length=281, Percent_Identity=35.5871886120996, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51708; Mature: 51708

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRRTKIVATLGPASKEPEILRSMLAAGVNVVRINFSHADSSALQLIALVRKIADELNHP
CCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCC
VAVMADLQGPKIRVGRFQNKSITLIDGQNFTLDCMAPDTLGDINGVSVAYPNLANELSIG
EEEEEECCCCEEEEEEECCCEEEEECCCCEEEEEECCCCCCCCCCCEEECCCCCCCCCCC
DHLLINDGLIELEVIEISGSKIHCKVVEGGVLTDLKGLNRKGGGLAARTLTEKDRNDLRT
CEEEEECCEEEEEEEEECCCEEEEEEECCCCEECHHCCCCCCCCEEEEHHHHHHHHHHHH
AIEAEVDYISLSFVKDAEDIRQARALMKDYGAQITPIIAKIERMEALDHLTDIIREADAI
HHHHHCCEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEE
MVARGDLGVEVGAAEVPAIQKHIIEQTRLLDKVVITATQMMESMISNPQPTRAEVSDVAN
EEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
AILDGTDAVMLSAETASGLFPVKVITMVNKICLSAEKHASFFYHSDPETCHYQRADQAIA
HHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHCCEEECCCCCCCHHHHHHHHHH
MATMHTANHFPIQAIITLTESGDTALWVSRHHSTVPIFAISANKRTIGRLSLVNNVFPIY
HHHHHCCCCCCEEEEEEEECCCCEEEEEECCCCEEEEEEEECCCCCHHHHHHHCCCEEEE
IDFHQFNPEGLNQQILHELIKSGHLEKRGYVLLTRGTQIGMPGGTNCMEIIPVV
EEEEECCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCEECCCCCCCHHEEEECC
>Mature Secondary Structure
MLRRTKIVATLGPASKEPEILRSMLAAGVNVVRINFSHADSSALQLIALVRKIADELNHP
CCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCC
VAVMADLQGPKIRVGRFQNKSITLIDGQNFTLDCMAPDTLGDINGVSVAYPNLANELSIG
EEEEEECCCCEEEEEEECCCEEEEECCCCEEEEEECCCCCCCCCCCEEECCCCCCCCCCC
DHLLINDGLIELEVIEISGSKIHCKVVEGGVLTDLKGLNRKGGGLAARTLTEKDRNDLRT
CEEEEECCEEEEEEEEECCCEEEEEEECCCCEECHHCCCCCCCCEEEEHHHHHHHHHHHH
AIEAEVDYISLSFVKDAEDIRQARALMKDYGAQITPIIAKIERMEALDHLTDIIREADAI
HHHHHCCEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEE
MVARGDLGVEVGAAEVPAIQKHIIEQTRLLDKVVITATQMMESMISNPQPTRAEVSDVAN
EEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
AILDGTDAVMLSAETASGLFPVKVITMVNKICLSAEKHASFFYHSDPETCHYQRADQAIA
HHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHCCEEECCCCCCCHHHHHHHHHH
MATMHTANHFPIQAIITLTESGDTALWVSRHHSTVPIFAISANKRTIGRLSLVNNVFPIY
HHHHHCCCCCCEEEEEEEECCCCEEEEEECCCCEEEEEEEECCCCCHHHHHHHCCCEEEE
IDFHQFNPEGLNQQILHELIKSGHLEKRGYVLLTRGTQIGMPGGTNCMEIIPVV
EEEEECCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCEECCCCCCCHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]