Click here to switch to the map view.

The map label for this gene is pepT

Identifier: 52787854

GI number: 52787854

Start: 4004072

End: 4005304

Strand: Direct

Name: pepT

Synonym: BLi04177

Alternate gene names: 52787854

Gene position: 4004072-4005304 (Clockwise)

Preceding gene: 52787844

Following gene: 52787860

Centisome position: 94.82

GC content: 46.8

Gene sequence:

>1233_bases
ATGAAGAACAAATTGATTGAACGCCTCATTTCTTATGCGAAAGTGGATACGCAGTCAAATGAAAACAGTCAGACGACGCC
TTCCACTCCCGGGCAGCTGGCGCTTGCCAATATGCTTGTCGAAGAGCTGAAAGAGATCGGCATGAAGGACGTCACAATCG
ATGAAAACGGCTATGTGATGGCGACGCTTCCTTCGAATACAGAAAAAGAGGTGCCGACGATCGGTTTCTTGGCTCATGTG
GATACAGCAACAGATTTTACCGGAAAGAACGTCAATCCGCAGGTTATCGAACAATACGACGGAAAGGATATTGTGCTGAA
TGAATCCCTCAATGTCGTCTTATCGCCCAAGGAATTCCCCGAGCTAGCAGATTATGCGGGACATACGTTGATTACAACCG
ACGGAACGACTTTGCTCGGCGCCGACAATAAAGCTGGGATCTCTGAAATCATGACGGCAATGGAATATTTAATTGCGCAC
CCCGAAATCAAACACGGGAAGATCAGAGTCGCTTTTACACCTGATGAAGAGATCGGCAGAGGGCCGCACAAGTTTGACGT
CGAGGCGTTCAATGCCAAATTCGCGTATACGGTTGACGGCGGACCGCTTGGCGAACTGCAGTACGAAAGCTTCAATGCCG
CAGCCGCGAAAATCACTTGTAAAGGAACGAACGTCCACCCCGGCACAGCAAAAGGAAAAATGGTCAATGCCGCCAAAATC
GCGATGCAGTTCCACGCCGCATTGCCGGAAAACGAAGCTCCTGAATTTACAGAAGGTTATGAAGGCTTTTATCATCTGCT
TTCAATCAAGGGCGATGTTTCTGAAACGAGCCTCTCTTATATCATTAGAGATTTTGACAGAGACCGATTTAACGAGAGAA
AAGACACCGTCCAAAAAATCGCAAACAACCTTAAAGCGAAATACGGCGAAAACAGCGTCACAGTGGACATGAATGATCAA
TATTACAACATGAGGGAAAAGATCGAACCGGTCAAAGAGATTGTCGACATTGCCTATAAAGCGATGAAAAACCTTGATAT
TGAACCGGTCGTTAAACCGATCCGCGGCGGTACAGACGGCTCTCAGCTCTCATATATGGGGCTTCCTTGTCCGAACATTT
TCACAGGCGGGGAAAACTTTCACGGAAAATACGAGTATATTTCCGCCGACAATATGGTAAAAGCCGCGAATGTCATCGTG
GAAATTGTGAAGCTGTTTGAAGAAAGAGCTTAA

Upstream 100 bases:

>100_bases
TAAATTTTTCCACAACAGGAATAAAAGATTTCAATCCTCCCATTCGCCTTCTCTAAATGATAAGATTAAAATAAAACCAA
CAATTGGAGATGAAGGAAAC

Downstream 100 bases:

>100_bases
ACGAAAAAAGCGGAAAGCGGCTGCAAGACAAAGCAGCCGCTTTTTTTTCCAGATCAATCTTTATCTGCGCTTTGCTGAAA
TTAGAACATGTTGCAAGGCG

Product: peptidase T

Products: NA

Alternate protein names: Aminotripeptidase; Tripeptidase; Tripeptide aminopeptidase

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MKNKLIERLISYAKVDTQSNENSQTTPSTPGQLALANMLVEELKEIGMKDVTIDENGYVMATLPSNTEKEVPTIGFLAHV
DTATDFTGKNVNPQVIEQYDGKDIVLNESLNVVLSPKEFPELADYAGHTLITTDGTTLLGADNKAGISEIMTAMEYLIAH
PEIKHGKIRVAFTPDEEIGRGPHKFDVEAFNAKFAYTVDGGPLGELQYESFNAAAAKITCKGTNVHPGTAKGKMVNAAKI
AMQFHAALPENEAPEFTEGYEGFYHLLSIKGDVSETSLSYIIRDFDRDRFNERKDTVQKIANNLKAKYGENSVTVDMNDQ
YYNMREKIEPVKEIVDIAYKAMKNLDIEPVVKPIRGGTDGSQLSYMGLPCPNIFTGGENFHGKYEYISADNMVKAANVIV
EIVKLFEERA

Sequences:

>Translated_410_residues
MKNKLIERLISYAKVDTQSNENSQTTPSTPGQLALANMLVEELKEIGMKDVTIDENGYVMATLPSNTEKEVPTIGFLAHV
DTATDFTGKNVNPQVIEQYDGKDIVLNESLNVVLSPKEFPELADYAGHTLITTDGTTLLGADNKAGISEIMTAMEYLIAH
PEIKHGKIRVAFTPDEEIGRGPHKFDVEAFNAKFAYTVDGGPLGELQYESFNAAAAKITCKGTNVHPGTAKGKMVNAAKI
AMQFHAALPENEAPEFTEGYEGFYHLLSIKGDVSETSLSYIIRDFDRDRFNERKDTVQKIANNLKAKYGENSVTVDMNDQ
YYNMREKIEPVKEIVDIAYKAMKNLDIEPVVKPIRGGTDGSQLSYMGLPCPNIFTGGENFHGKYEYISADNMVKAANVIV
EIVKLFEERA
>Mature_410_residues
MKNKLIERLISYAKVDTQSNENSQTTPSTPGQLALANMLVEELKEIGMKDVTIDENGYVMATLPSNTEKEVPTIGFLAHV
DTATDFTGKNVNPQVIEQYDGKDIVLNESLNVVLSPKEFPELADYAGHTLITTDGTTLLGADNKAGISEIMTAMEYLIAH
PEIKHGKIRVAFTPDEEIGRGPHKFDVEAFNAKFAYTVDGGPLGELQYESFNAAAAKITCKGTNVHPGTAKGKMVNAAKI
AMQFHAALPENEAPEFTEGYEGFYHLLSIKGDVSETSLSYIIRDFDRDRFNERKDTVQKIANNLKAKYGENSVTVDMNDQ
YYNMREKIEPVKEIVDIAYKAMKNLDIEPVVKPIRGGTDGSQLSYMGLPCPNIFTGGENFHGKYEYISADNMVKAANVIV
EIVKLFEERA

Specific function: Cleaves the N-terminal amino acid of tripeptides

COG id: COG2195

COG function: function code E; Di- and tripeptidases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20B family

Homologues:

Organism=Escherichia coli, GI1787372, Length=408, Percent_Identity=54.9019607843137, Blast_Score=463, Evalue=1e-132,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PEPT_BACLD (Q65D74)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_081249.1
- RefSeq:   YP_093683.1
- ProteinModelPortal:   Q65D74
- SMR:   Q65D74
- STRING:   Q65D74
- MEROPS:   M20.003
- EnsemblBacteria:   EBBACT00000055007
- EnsemblBacteria:   EBBACT00000059212
- GeneID:   3028173
- GeneID:   3097513
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi04177
- KEGG:   bli:BL01918
- NMPDR:   fig|279010.5.peg.3069
- eggNOG:   COG2195
- GeneTree:   EBGT00050000000947
- HOGENOM:   HBG288638
- OMA:   HMDTAPD
- ProtClustDB:   PRK05469
- BioCyc:   BLIC279010-1:BLI04177-MONOMER
- BioCyc:   BLIC279010:BL01918-MONOMER
- GO:   GO:0005737
- GO:   GO:0006508
- HAMAP:   MF_00550
- InterPro:   IPR001261
- InterPro:   IPR002933
- InterPro:   IPR011650
- InterPro:   IPR010161
- PIRSF:   PIRSF037215
- TIGRFAMs:   TIGR01882

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.4.11.4

Molecular weight: Translated: 45327; Mature: 45327

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 81-81 ACT_SITE 176-176

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKLIERLISYAKVDTQSNENSQTTPSTPGQLALANMLVEELKEIGMKDVTIDENGYVM
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCEEE
ATLPSNTEKEVPTIGFLAHVDTATDFTGKNVNPQVIEQYDGKDIVLNESLNVVLSPKEFP
EECCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHCCCCEEEEECCCCEEECCHHCH
ELADYAGHTLITTDGTTLLGADNKAGISEIMTAMEYLIAHPEIKHGKIRVAFTPDEEIGR
HHHHHCCCEEEEECCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHCC
GPHKFDVEAFNAKFAYTVDGGPLGELQYESFNAAAAKITCKGTNVHPGTAKGKMVNAAKI
CCCEECEEEECCEEEEEECCCCCCCEEECCCCCEEEEEEEECCCCCCCCCCCCEEHHHHH
AMQFHAALPENEAPEFTEGYEGFYHLLSIKGDVSETSLSYIIRDFDRDRFNERKDTVQKI
HHHHHHCCCCCCCCCHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ANNLKAKYGENSVTVDMNDQYYNMREKIEPVKEIVDIAYKAMKNLDIEPVVKPIRGGTDG
HHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCC
SQLSYMGLPCPNIFTGGENFHGKYEYISADNMVKAANVIVEIVKLFEERA
CCEEEECCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKNKLIERLISYAKVDTQSNENSQTTPSTPGQLALANMLVEELKEIGMKDVTIDENGYVM
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCEEE
ATLPSNTEKEVPTIGFLAHVDTATDFTGKNVNPQVIEQYDGKDIVLNESLNVVLSPKEFP
EECCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHCCCCEEEEECCCCEEECCHHCH
ELADYAGHTLITTDGTTLLGADNKAGISEIMTAMEYLIAHPEIKHGKIRVAFTPDEEIGR
HHHHHCCCEEEEECCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHCC
GPHKFDVEAFNAKFAYTVDGGPLGELQYESFNAAAAKITCKGTNVHPGTAKGKMVNAAKI
CCCEECEEEECCEEEEEECCCCCCCEEECCCCCEEEEEEEECCCCCCCCCCCCEEHHHHH
AMQFHAALPENEAPEFTEGYEGFYHLLSIKGDVSETSLSYIIRDFDRDRFNERKDTVQKI
HHHHHHCCCCCCCCCHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ANNLKAKYGENSVTVDMNDQYYNMREKIEPVKEIVDIAYKAMKNLDIEPVVKPIRGGTDG
HHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCC
SQLSYMGLPCPNIFTGGENFHGKYEYISADNMVKAANVIVEIVKLFEERA
CCEEEECCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA