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The map label for this gene is rbsB [H]

Identifier: 52787525

GI number: 52787525

Start: 3673134

End: 3674048

Strand: Direct

Name: rbsB [H]

Synonym: BLi03845

Alternate gene names: 52787525

Gene position: 3673134-3674048 (Clockwise)

Preceding gene: 52787524

Following gene: 52787528

Centisome position: 86.99

GC content: 50.27

Gene sequence:

>915_bases
ATGAAAAAAACATTAACGGTTTTCACCGCATTGGCGCTTCTGTTTCTGTCCGCCTGCTCGCTGGAGCCGCCGGAATGGGC
AAAGCCGAATAAAGAAAAAGGAACGGATATTAAAATCGGTTTATCTGTCTCAACTTTGAAAAATCCGTTTTTCGTTTCAT
TAAAGAACGGAGTTGTCAAAGAAGCCAAAAAACAAGGGATAGAAGTGATTGTCGTCGATGCACAGGACGATTCGGCAAAG
CAGACGAATGATGTCGAAGATCTTCTGCAGCAAGGCGTTGACGCTTTATTGATCAACCCGGCGGACTCCTCCGCCATCTC
AACTGCAGTCCAATCGGCCAATTCGCTTGGCATTCCGGTCGTCACCCTTGACCGCTCCGCCGAAAACGGAAAAGTAGAAA
CGCTCGTCGCTTCAGATAACGTAAAAGGCGGACAAATGGCAGCGGACTTTATTGTGGAGCAGCTCGGCAAAGGCGCAAAA
GTGGCTGAGCTTGAAGGCGTCCCCGGCGCATCGGCAACAAGGGAACGGGGCTCAGGGTTTCATAAGACCGCTGATGACAA
GCTGAATGTGATCGCCAAGCAAACAGCCGACTTTGACCGGACAAAAGGGTTGAACGTCATGGAGAACCTCCTTCAAGGAA
ACCCTGATATTCAAGCGGTTTTCGCACATAATGACGAAATGGCGCTCGGCGCGATTGAAGCGATCAGAAGCTCAGGGAAA
GAGATTCTTGTCGTCGGCTTCGACGGCAATGAAGATGCGGTTAAAGCGGTTCAGGCCGGAGACATGTCGGCAACGATCGC
CCAGCAGCCTGAATTGATCGGCAAGCTCGCCGTACAAGCGGCCCGCGATATTTTGAACGGCAAAAAAGTTGAAAAAACGA
TACCCGCACCGCTCAAACTGGAAACGAAAAAGTAA

Upstream 100 bases:

>100_bases
TTGGCGTCTCTTCCTTTTTCCAAATGGTCGTCAAAGGTGTCGTCATTTTGATCGCAGTCCTGCTGGACCGCAAGAAATCC
GCTTAAGGAGGGCTATACTG

Downstream 100 bases:

>100_bases
CTGTGAAATCAGACCTCTTGATACATGGAGGTCTGATTTTTTTTGGCCACCTCCATAAAAGCCCTTGCCGCCCGGCTCAA
ATGGCATTTTTCAGGATAAG

Product: hypothetical protein

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MKKTLTVFTALALLFLSACSLEPPEWAKPNKEKGTDIKIGLSVSTLKNPFFVSLKNGVVKEAKKQGIEVIVVDAQDDSAK
QTNDVEDLLQQGVDALLINPADSSAISTAVQSANSLGIPVVTLDRSAENGKVETLVASDNVKGGQMAADFIVEQLGKGAK
VAELEGVPGASATRERGSGFHKTADDKLNVIAKQTADFDRTKGLNVMENLLQGNPDIQAVFAHNDEMALGAIEAIRSSGK
EILVVGFDGNEDAVKAVQAGDMSATIAQQPELIGKLAVQAARDILNGKKVEKTIPAPLKLETKK

Sequences:

>Translated_304_residues
MKKTLTVFTALALLFLSACSLEPPEWAKPNKEKGTDIKIGLSVSTLKNPFFVSLKNGVVKEAKKQGIEVIVVDAQDDSAK
QTNDVEDLLQQGVDALLINPADSSAISTAVQSANSLGIPVVTLDRSAENGKVETLVASDNVKGGQMAADFIVEQLGKGAK
VAELEGVPGASATRERGSGFHKTADDKLNVIAKQTADFDRTKGLNVMENLLQGNPDIQAVFAHNDEMALGAIEAIRSSGK
EILVVGFDGNEDAVKAVQAGDMSATIAQQPELIGKLAVQAARDILNGKKVEKTIPAPLKLETKK
>Mature_304_residues
MKKTLTVFTALALLFLSACSLEPPEWAKPNKEKGTDIKIGLSVSTLKNPFFVSLKNGVVKEAKKQGIEVIVVDAQDDSAK
QTNDVEDLLQQGVDALLINPADSSAISTAVQSANSLGIPVVTLDRSAENGKVETLVASDNVKGGQMAADFIVEQLGKGAK
VAELEGVPGASATRERGSGFHKTADDKLNVIAKQTADFDRTKGLNVMENLLQGNPDIQAVFAHNDEMALGAIEAIRSSGK
EILVVGFDGNEDAVKAVQAGDMSATIAQQPELIGKLAVQAARDILNGKKVEKTIPAPLKLETKK

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=306, Percent_Identity=50.6535947712418, Blast_Score=282, Evalue=2e-77,
Organism=Escherichia coli, GI1790526, Length=312, Percent_Identity=34.9358974358974, Blast_Score=147, Evalue=8e-37,
Organism=Escherichia coli, GI1790674, Length=253, Percent_Identity=34.3873517786561, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1788473, Length=310, Percent_Identity=25.1612903225806, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1789990, Length=275, Percent_Identity=27.2727272727273, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790194, Length=264, Percent_Identity=25.3787878787879, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1786268, Length=226, Percent_Identity=25.2212389380531, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788898, Length=285, Percent_Identity=25.2631578947368, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787906, Length=212, Percent_Identity=26.8867924528302, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 32083; Mature: 32083

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTLTVFTALALLFLSACSLEPPEWAKPNKEKGTDIKIGLSVSTLKNPFFVSLKNGVVK
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEHHHHCCCEEEEECHHHHH
EAKKQGIEVIVVDAQDDSAKQTNDVEDLLQQGVDALLINPADSSAISTAVQSANSLGIPV
HHHHCCCEEEEEECCCCCCCCCCCHHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCCCEE
VTLDRSAENGKVETLVASDNVKGGQMAADFIVEQLGKGAKVAELEGVPGASATRERGSGF
EEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHCCCC
HKTADDKLNVIAKQTADFDRTKGLNVMENLLQGNPDIQAVFAHNDEMALGAIEAIRSSGK
CCCCCCHHHHHHHHHCCCHHHCCCHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCC
EILVVGFDGNEDAVKAVQAGDMSATIAQQPELIGKLAVQAARDILNGKKVEKTIPAPLKL
EEEEEEECCCHHHHHHHHCCCCCHHHHHCHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEE
ETKK
CCCC
>Mature Secondary Structure
MKKTLTVFTALALLFLSACSLEPPEWAKPNKEKGTDIKIGLSVSTLKNPFFVSLKNGVVK
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEHHHHCCCEEEEECHHHHH
EAKKQGIEVIVVDAQDDSAKQTNDVEDLLQQGVDALLINPADSSAISTAVQSANSLGIPV
HHHHCCCEEEEEECCCCCCCCCCCHHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCCCEE
VTLDRSAENGKVETLVASDNVKGGQMAADFIVEQLGKGAKVAELEGVPGASATRERGSGF
EEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHCCCC
HKTADDKLNVIAKQTADFDRTKGLNVMENLLQGNPDIQAVFAHNDEMALGAIEAIRSSGK
CCCCCCHHHHHHHHHCCCHHHCCCHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCC
EILVVGFDGNEDAVKAVQAGDMSATIAQQPELIGKLAVQAARDILNGKKVEKTIPAPLKL
EEEEEEECCCHHHHHHHHCCCCCHHHHHCHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEE
ETKK
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]