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The map label for this gene is acpD

Identifier: 52787262

GI number: 52787262

Start: 3417332

End: 3417967

Strand: Reverse

Name: acpD

Synonym: BLi03573

Alternate gene names: 52787262

Gene position: 3417967-3417332 (Counterclockwise)

Preceding gene: 52787263

Following gene: 52787260

Centisome position: 80.94

GC content: 47.64

Gene sequence:

>636_bases
ATGACAAAAACCCTTTACATTACCGCTCACCCGCATGACGAAAGGGCATCCTACAGCATGGCGGCAGGAAAAGCGTTTAT
CGAATCTTACAAAGAAGCTCATCCGGATGACGAAGTCATACACCTTGATCTTTATCGGGAAAACATTCCGCAGATTGATG
CGGATGTTTTCAGCGGCTGGGGAAAATTGCAGTCGGGGTCGGGCTTTGAACAGCTTTCTGAACACGAAAAAGCTAAAGTC
GGCCGTTTGAATGAGCTGAGCGAGCAATTTATCGCGGGAGACAAATACGTGTTTGTCACGCCGCTTTGGAATTTCTCTTT
TCCGCCGGTGATGAAAGCGTACTTCGATGCGGTCGCTGTAGCGGGAAAGACGTTTAAATATACCGAACAGGGACCGATCG
GGCTGTTAACCGATAAAAAAGCGCTGCACATCCAGGCGAGAGGCGGCTACTATTCAGAAGGGCAGGCTGCGGAACTTGAG
ATGGGGCACCGCTATATCAGCATCATGATGCAGTTTTTCGGGGTTCCGGAATTTGAAGGTTTATTTATAGAAGGGCATAA
TGCTGAGCCTGATAAAGCTGAAGAAATAAAACAAAATGCGATTGTCCGAGCCAAAGAATTGGGCAGGACTTTTTGA

Upstream 100 bases:

>100_bases
CATCTGCACTTTATGCGAAAGCTCCGGCTTTTCCAGAATATTTGCCTGAAAACGTCCCATACTAAGCAATATCAATACAA
AAAGGCAGGTAGCAAAAGAA

Downstream 100 bases:

>100_bases
AAAAACCGCGGATCATATCCGCGGTTTTGTTTTATTTGTTTAAGAACACCGTTTGATTCGTTTCGCTGCTTTTTTGCGCA
GCTTCGATGATTCGGATGAC

Product: azoreductase

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase 2; FMN-dependent NADH-azo compound oxidoreductase 2

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MTKTLYITAHPHDERASYSMAAGKAFIESYKEAHPDDEVIHLDLYRENIPQIDADVFSGWGKLQSGSGFEQLSEHEKAKV
GRLNELSEQFIAGDKYVFVTPLWNFSFPPVMKAYFDAVAVAGKTFKYTEQGPIGLLTDKKALHIQARGGYYSEGQAAELE
MGHRYISIMMQFFGVPEFEGLFIEGHNAEPDKAEEIKQNAIVRAKELGRTF

Sequences:

>Translated_211_residues
MTKTLYITAHPHDERASYSMAAGKAFIESYKEAHPDDEVIHLDLYRENIPQIDADVFSGWGKLQSGSGFEQLSEHEKAKV
GRLNELSEQFIAGDKYVFVTPLWNFSFPPVMKAYFDAVAVAGKTFKYTEQGPIGLLTDKKALHIQARGGYYSEGQAAELE
MGHRYISIMMQFFGVPEFEGLFIEGHNAEPDKAEEIKQNAIVRAKELGRTF
>Mature_210_residues
TKTLYITAHPHDERASYSMAAGKAFIESYKEAHPDDEVIHLDLYRENIPQIDADVFSGWGKLQSGSGFEQLSEHEKAKVG
RLNELSEQFIAGDKYVFVTPLWNFSFPPVMKAYFDAVAVAGKTFKYTEQGPIGLLTDKKALHIQARGGYYSEGQAAELEM
GHRYISIMMQFFGVPEFEGLFIEGHNAEPDKAEEIKQNAIVRAKELGRTF

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family

Homologues:

Organism=Escherichia coli, GI1787680, Length=175, Percent_Identity=33.1428571428571, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AZOR2_BACLD (Q65EW6)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_080665.1
- RefSeq:   YP_093091.1
- ProteinModelPortal:   Q65EW6
- SMR:   Q65EW6
- EnsemblBacteria:   EBBACT00000057692
- EnsemblBacteria:   EBBACT00000062331
- GeneID:   3028100
- GeneID:   3100285
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi03573
- KEGG:   bli:BL00833
- NMPDR:   fig|279010.5.peg.2032
- eggNOG:   COG1182
- GeneTree:   EBGT00050000000772
- HOGENOM:   HBG565834
- OMA:   FSGWGKL
- ProtClustDB:   PRK00170
- BioCyc:   BLIC279010-1:BLI03573-MONOMER
- BioCyc:   BLIC279010:BL00833-MONOMER
- HAMAP:   MF_01216
- InterPro:   IPR003680
- InterPro:   IPR023048

Pfam domain/function: PF02525 Flavodoxin_2

EC number: 3.1.4.14

Molecular weight: Translated: 23776; Mature: 23645

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTLYITAHPHDERASYSMAAGKAFIESYKEAHPDDEVIHLDLYRENIPQIDADVFSGW
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHCCC
GKLQSGSGFEQLSEHEKAKVGRLNELSEQFIAGDKYVFVTPLWNFSFPPVMKAYFDAVAV
CCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHH
AGKTFKYTEQGPIGLLTDKKALHIQARGGYYSEGQAAELEMGHRYISIMMQFFGVPEFEG
CCCEEEECCCCCEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCE
LFIEGHNAEPDKAEEIKQNAIVRAKELGRTF
EEEECCCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TKTLYITAHPHDERASYSMAAGKAFIESYKEAHPDDEVIHLDLYRENIPQIDADVFSGW
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHCCC
GKLQSGSGFEQLSEHEKAKVGRLNELSEQFIAGDKYVFVTPLWNFSFPPVMKAYFDAVAV
CCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHH
AGKTFKYTEQGPIGLLTDKKALHIQARGGYYSEGQAAELEMGHRYISIMMQFFGVPEFEG
CCCEEEECCCCCEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCE
LFIEGHNAEPDKAEEIKQNAIVRAKELGRTF
EEEECCCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA