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The map label for this gene is gatA [H]

Identifier: 52787260

GI number: 52787260

Start: 3415396

End: 3416061

Strand: Reverse

Name: gatA [H]

Synonym: BLi03571

Alternate gene names: 52787260

Gene position: 3416061-3415396 (Counterclockwise)

Preceding gene: 52787262

Following gene: 52787259

Centisome position: 80.9

GC content: 54.05

Gene sequence:

>666_bases
ATGGAGCATGATTGGAGCGCTTTTATAAACCGGGATCTGATGGTCGATCCGCCATCGGCTGAAGGAAGCTTGAAAAAACT
TTCGTTTGCGGTAAAGGATGTGTTTGCTGTAGAGGGCCATGCTAATGCGGCGGGAAACCCTGATTGGCTGAGGACGCACG
AACCTGCCGAAAAAAACGCGGAAGCCGTTGACTTGCTGCTGCGGGAAGGTGCAAGGCTTAAGGGTGCTGCGCACACAGAT
GAGCTGATGTACAGCCTCGACGGAGAAAATGTCCATTATGGTACACCCGTCAATCCTTGTGCCATGGACAAAATTCCGGG
AGGGTCTTCAAGCGGATCGGCTGTGGCGGCAGCCTCTGGGATGACCGATTTCGCTCTCGGGACAGACACCGGGGGATCTG
TCAGAATCCCTTCTTCATACTGCGGAATTTTCGGGTTTCGGCCGACGCACGGCGAGGTGTCGGTTGATGGTGTCATACCG
CTGGCAAAGAGTTTTGACACCGTCGGATGGATGTCAAAAGATATTGGAGTCCTTCATGCGGCGGGCCGCGTCCTGCTTAG
CGGGCAGGAGGAGGCAGGGGCATGTTCAACCGGGTTTATTTTGAAAAAGAGGCGTGGAGCCTGCTTGAAGAAAGCGACCG
GACACAAGTATACGCTTATGCATTAG

Upstream 100 bases:

>100_bases
TTTTTAAATTAGGGAACGGAAGATTTTGTCACAACTTTGTTCATTTTGAAGAGGCGTCCGGTTATCGTTAGAAGCAAAAG
TTGATGGAGGGATTTTGAAA

Downstream 100 bases:

>100_bases
GCCTGATTGACGGCGAATTCGACTGGTGCGTTGCCGCTGACGGCGGCCTCGCGGAATGGGCGGAGACGTTCAGGGTGCTG
CAGGGACTGGAGATTTGGGA

Product: hypothetical protein

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MEHDWSAFINRDLMVDPPSAEGSLKKLSFAVKDVFAVEGHANAAGNPDWLRTHEPAEKNAEAVDLLLREGARLKGAAHTD
ELMYSLDGENVHYGTPVNPCAMDKIPGGSSSGSAVAAASGMTDFALGTDTGGSVRIPSSYCGIFGFRPTHGEVSVDGVIP
LAKSFDTVGWMSKDIGVLHAAGRVLLSGQEEAGACSTGFILKKRRGACLKKATGHKYTLMH

Sequences:

>Translated_221_residues
MEHDWSAFINRDLMVDPPSAEGSLKKLSFAVKDVFAVEGHANAAGNPDWLRTHEPAEKNAEAVDLLLREGARLKGAAHTD
ELMYSLDGENVHYGTPVNPCAMDKIPGGSSSGSAVAAASGMTDFALGTDTGGSVRIPSSYCGIFGFRPTHGEVSVDGVIP
LAKSFDTVGWMSKDIGVLHAAGRVLLSGQEEAGACSTGFILKKRRGACLKKATGHKYTLMH
>Mature_221_residues
MEHDWSAFINRDLMVDPPSAEGSLKKLSFAVKDVFAVEGHANAAGNPDWLRTHEPAEKNAEAVDLLLREGARLKGAAHTD
ELMYSLDGENVHYGTPVNPCAMDKIPGGSSSGSAVAAASGMTDFALGTDTGGSVRIPSSYCGIFGFRPTHGEVSVDGVIP
LAKSFDTVGWMSKDIGVLHAAGRVLLSGQEEAGACSTGFILKKRRGACLKKATGHKYTLMH

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=136, Percent_Identity=34.5588235294118, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI222831590, Length=172, Percent_Identity=34.3023255813954, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=177, Percent_Identity=36.1581920903955, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=171, Percent_Identity=30.9941520467836, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17556264, Length=127, Percent_Identity=33.0708661417323, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17537465, Length=147, Percent_Identity=29.9319727891156, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=133, Percent_Identity=42.1052631578947, Blast_Score=83, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6319685, Length=164, Percent_Identity=35.9756097560976, Blast_Score=73, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21356731, Length=129, Percent_Identity=37.984496124031, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45550774, Length=131, Percent_Identity=35.1145038167939, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648435, Length=131, Percent_Identity=35.1145038167939, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648437, Length=131, Percent_Identity=35.1145038167939, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648441, Length=131, Percent_Identity=35.1145038167939, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648439, Length=131, Percent_Identity=35.1145038167939, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI161078093, Length=140, Percent_Identity=37.8571428571429, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24644968, Length=132, Percent_Identity=31.8181818181818, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 23316; Mature: 23316

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHDWSAFINRDLMVDPPSAEGSLKKLSFAVKDVFAVEGHANAAGNPDWLRTHEPAEKNA
CCCCHHHHHCCCEEECCCCCCCCHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCH
EAVDLLLREGARLKGAAHTDELMYSLDGENVHYGTPVNPCAMDKIPGGSSSGSAVAAASG
HHHHHHHHCCCCCCCCCCCCCEEEEECCCEEEECCCCCCHHCCCCCCCCCCCCEEEEECC
MTDFALGTDTGGSVRIPSSYCGIFGFRPTHGEVSVDGVIPLAKSFDTVGWMSKDIGVLHA
CCEEEECCCCCCCEECCHHHCCCCCCCCCCCCEEECCEEHHHHCCCHHHCCCCCCHHHHH
AGRVLLSGQEEAGACSTGFILKKRRGACLKKATGHKYTLMH
CCEEEEECCCCCCCCCCCCEEECCCCCHHHHCCCCEEEECC
>Mature Secondary Structure
MEHDWSAFINRDLMVDPPSAEGSLKKLSFAVKDVFAVEGHANAAGNPDWLRTHEPAEKNA
CCCCHHHHHCCCEEECCCCCCCCHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCH
EAVDLLLREGARLKGAAHTDELMYSLDGENVHYGTPVNPCAMDKIPGGSSSGSAVAAASG
HHHHHHHHCCCCCCCCCCCCCEEEEECCCEEEECCCCCCHHCCCCCCCCCCCCEEEEECC
MTDFALGTDTGGSVRIPSSYCGIFGFRPTHGEVSVDGVIPLAKSFDTVGWMSKDIGVLHA
CCEEEECCCCCCCEECCHHHCCCCCCCCCCCCEEECCEEHHHHCCCHHHCCCCCCHHHHH
AGRVLLSGQEEAGACSTGFILKKRRGACLKKATGHKYTLMH
CCEEEEECCCCCCCCCCCCEEECCCCCHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]