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The map label for this gene is maeN [H]

Identifier: 52787038

GI number: 52787038

Start: 3196948

End: 3198291

Strand: Direct

Name: maeN [H]

Synonym: BLi03342

Alternate gene names: 52787038

Gene position: 3196948-3198291 (Clockwise)

Preceding gene: 52787035

Following gene: 52787040

Centisome position: 75.71

GC content: 47.62

Gene sequence:

>1344_bases
ATGGAAGCAGCAAAAGATTTGAAACCGATTACAAATCACGGTCATAAAGAAAATAAAAATATTTTCCATAAGCTGATCAA
CTGGAAAATCGGCGTGATTCCGTTTCCGCTATATATTGCGCTTGCGCTGATTGTTTTTCTGGCAGCTTACTTTAATGAAT
TGCCGAATGACATGCTCGGCGGTTTCGCGGTCATTATGATTCTCGGCATTTTTCTCGGGGATGTCGGACAGCGCATTCCG
ATTTTAAAAGATATCGGCGGACCTGCCATTCTATCGCTGTTTGTTCCTTCGTTCTTAGTCTTTTTCAAAGTGCTTAACCC
TGCATCTATGGAAGCGGTTACAACTTTGATGAAAACGTCAAATTTCTTGTATTTCTACATCGCATGTTTAGTCGCGGGAA
GTATTTTAGGAATGCATCGCACGGTTCTGATTCAAGGTTTTATGAGAATGTTCGTTCCGTTGTTGGCCGGGACGATCGCA
GCGGTTGCCGCCGGTATTCTCGTCGGTCTGTTATTTGGCTACAGCCCTTATGAAGCTTTCTTCTTTATTATTGTACCGAT
TATTGCCGGAGGCATCGGCGAAGGCATCCTGCCGCTGTCACTCGCCTACTCGCAAATTTTGGGACAGTCCACCGATGTAT
TTGTTTCACAGCTCATTCCGGCCGCTATTATCGGAAACGTCGTTGCCATCATCTCAGCCGGACTGATGAAAAAGCTTGGG
GAAAAGCGTCCTGAACTGAACGGTAACGGCAGACTCGTTAAATCGAAAAAAGACGATGAAATTTTCAACCAAAAAGAAGA
GGAGCCGAAAATCGACTTGAAATTAATGGGTGCCGGCGTTTTAATCGCATGTTCCTTCTTCATTTTCGGCGGTTTGGTCT
CCAAGTTCATCGCGATTCCGGGAGCGATCCTGATGATCATCGCAGCGGCTGCCGTAAAATATGCAAAGCTTCTGCCTGCA
AGCATGGAAAAAGGCGCTCACCAGCTTTACAAGTTCATGTCATCAAGCTTCACTTGGCCGCTGATGGTCGGGCTCGGCAT
TCTTTATATCCCTCTTGATGATGTGGCGTCTGTTATTTCCCTGCGCTTCATCGCCGTCTGCGCTTCAGTCGTCATTGCCA
TGGTGACATCAGGCTACTTTGTCGGAAAATTAATGAACATGTACCCTGTTGAATCAGCGATCGTCACAGGCTGTCACAGC
GGTCTCGGCGGCACGGGGGACGTGGCCATTCTTTCGGCTTCAGGCCGGATGGGTCTGATGCCGTTTGCGCAAATCTCAAC
GCGTCTCGGCGGGGCATCCACCGTCATTATGGCGACCGTTTTACTGAGGTTCTTCACCTCGTAA

Upstream 100 bases:

>100_bases
TTTTAATTTAATTATCAATCGATTATTTTAATTTAAAAAACGCACAGAATTGACACTTTTCTTCTACAATTGAACACATA
CAAACATGGGGGTGTTTTTT

Downstream 100 bases:

>100_bases
GGGCCGCATAAAAAAACAAAAAGGAGTCTGATCTATACGATCGGATTCCTTTTTTGTTTTCAGCGTTTCTCAACTCTGAT
ATATTCTCTCGGTTTAAAGG

Product: MaeN

Products: NA

Alternate protein names: Sodium-dependent malate transporter [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MEAAKDLKPITNHGHKENKNIFHKLINWKIGVIPFPLYIALALIVFLAAYFNELPNDMLGGFAVIMILGIFLGDVGQRIP
ILKDIGGPAILSLFVPSFLVFFKVLNPASMEAVTTLMKTSNFLYFYIACLVAGSILGMHRTVLIQGFMRMFVPLLAGTIA
AVAAGILVGLLFGYSPYEAFFFIIVPIIAGGIGEGILPLSLAYSQILGQSTDVFVSQLIPAAIIGNVVAIISAGLMKKLG
EKRPELNGNGRLVKSKKDDEIFNQKEEEPKIDLKLMGAGVLIACSFFIFGGLVSKFIAIPGAILMIIAAAAVKYAKLLPA
SMEKGAHQLYKFMSSSFTWPLMVGLGILYIPLDDVASVISLRFIAVCASVVIAMVTSGYFVGKLMNMYPVESAIVTGCHS
GLGGTGDVAILSASGRMGLMPFAQISTRLGGASTVIMATVLLRFFTS

Sequences:

>Translated_447_residues
MEAAKDLKPITNHGHKENKNIFHKLINWKIGVIPFPLYIALALIVFLAAYFNELPNDMLGGFAVIMILGIFLGDVGQRIP
ILKDIGGPAILSLFVPSFLVFFKVLNPASMEAVTTLMKTSNFLYFYIACLVAGSILGMHRTVLIQGFMRMFVPLLAGTIA
AVAAGILVGLLFGYSPYEAFFFIIVPIIAGGIGEGILPLSLAYSQILGQSTDVFVSQLIPAAIIGNVVAIISAGLMKKLG
EKRPELNGNGRLVKSKKDDEIFNQKEEEPKIDLKLMGAGVLIACSFFIFGGLVSKFIAIPGAILMIIAAAAVKYAKLLPA
SMEKGAHQLYKFMSSSFTWPLMVGLGILYIPLDDVASVISLRFIAVCASVVIAMVTSGYFVGKLMNMYPVESAIVTGCHS
GLGGTGDVAILSASGRMGLMPFAQISTRLGGASTVIMATVLLRFFTS
>Mature_447_residues
MEAAKDLKPITNHGHKENKNIFHKLINWKIGVIPFPLYIALALIVFLAAYFNELPNDMLGGFAVIMILGIFLGDVGQRIP
ILKDIGGPAILSLFVPSFLVFFKVLNPASMEAVTTLMKTSNFLYFYIACLVAGSILGMHRTVLIQGFMRMFVPLLAGTIA
AVAAGILVGLLFGYSPYEAFFFIIVPIIAGGIGEGILPLSLAYSQILGQSTDVFVSQLIPAAIIGNVVAIISAGLMKKLG
EKRPELNGNGRLVKSKKDDEIFNQKEEEPKIDLKLMGAGVLIACSFFIFGGLVSKFIAIPGAILMIIAAAAVKYAKLLPA
SMEKGAHQLYKFMSSSFTWPLMVGLGILYIPLDDVASVISLRFIAVCASVVIAMVTSGYFVGKLMNMYPVESAIVTGCHS
GLGGTGDVAILSASGRMGLMPFAQISTRLGGASTVIMATVLLRFFTS

Specific function: Acts as a Na(+)-malate symporter, as it catalyzes malate-dependent uptake of Na(+) and Na(+)-dependent uptake of malate [H]

COG id: COG3493

COG function: function code C; Na+/citrate symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:citrate (SCF) symporter family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004679
- InterPro:   IPR018025 [H]

Pfam domain/function: PF03390 2HCT [H]

EC number: NA

Molecular weight: Translated: 47807; Mature: 47807

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 10903309; 12949159 [H]