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The map label for this gene is mrpA [H]

Identifier: 52787040

GI number: 52787040

Start: 3198829

End: 3201231

Strand: Direct

Name: mrpA [H]

Synonym: BLi03345

Alternate gene names: 52787040

Gene position: 3198829-3201231 (Clockwise)

Preceding gene: 52787038

Following gene: 52787041

Centisome position: 75.75

GC content: 46.86

Gene sequence:

>2403_bases
ATGACGTCTTTACATATTGCTATTTTATCGCCTTTTGCACTCGCCTTCTTGATTCCGCTATTGGCGAAAAACGTCAGAAA
GATTCATACAGGCTGGTTTGTCCTGTTTCTTCCTGTTCTATTATTTATTTATTTTTTCCGCATGATCAAAATGACGGCGG
ATGGCGGAACATTAAAGGCTGTACTGGAATGGATTCCGTCCTTTGGGATTAACTTTACCGCGTACGTTGACGGACTCGGA
TTGCTATTCGCCCTTCTGATTACAGGGATCGGGTCTTTGGTTGTCCTTTACAGCATCTATTATCTGTCTAAAGAAAGAGA
GCAGCTCGGACCATTTTACGTTTATCTGCTCATGTTCATGGGGGCAATGCTCGGCGTTGTTCTCGTTGATAACGTCATTG
TACTTTATTTGTTTTGGGAATTGACCAGCATATCGTCTTTTCTGCTCATCGGCTATTGGTACAAGCGGGAAAAATCAAGA
TACGGCGCGACAAAATCGATGCTTATCACCATATTCGGCGGTCTTTCCATGCTTGGAGGCTTCATTCTTCTTTACACGAT
CACAGGTTCATTCTCGATTAGAGAAATGATCAACCAGGTTGATTTAATTTCGGGGCACGGCTTTTTCATCCCGGCTCTCG
TGCTGGTGTTAATCGGAGCCTTCACTAAATCTGCGCAGTTCCCTTTTTACATCTGGCTTCCGGACGCAATGGAAGCACCA
ACGCCTGTAAGTGCTTACCTGCACTCCGCCACGATGGTAAAAGCGGGAATTTATATCGTTGCAAGATTCACCCCTGTGTT
CGCTTTTTCAGGGGAATGGTTTTGGATCGTGTCTCTCACGGGGATTGTCACGCTTTTCTGGGGTTCGTTCAACGCCGTCA
AGCAGACTGATCTGAAATCGATACTTGCATTTTCTACGGTCAGCCAGCTCGGAATGATCATGTCGATGCTTGGCGTCGGA
GCGGCCGCACTGCATTTTCAGCATTTTGAATACTACATGGTTGCAGTAATCGCCGCTATCTTTCACTTGATTAACCATGC
GACTTTCAAAGGCAGCTTATTCATGGCAGTCGGAATCATCGACCATGAAACAGGTACGAGAGATATCCGCAAGCTCGGCG
GCCTTATGACGATCATGCCGATTACGTTTACGATTACGCTCATCGGCGCGTTTTCAATGGCCGGACTGCCTCCGTTCAAC
GGCTTTTTGAGCAAGGAAATGTTCTTCACCAGCATGCTCAGGATTACGAACTTTAACTTGTATAACGCTGACACATGGGG
TTTTCTCTTTCCTGTGCTGGCTTGGATCGGAAGCGTGTTTACATTCATTTACTGCATGGTTCTCTTATTTAAAACCTTCG
GGGGAAAATATCAGCCTGAAAAGCTTGAAAAACAAGCGCATGAGGCTCCAATCGGCATGCTGATTTCGCCGATTGTCCTC
GTATCTCTCGTGGTGATTTTTTTCTTTTTCCCGAACATTCTGTCATACAGCATCATTGAACCTGCTGCAGCGTCGATTAT
GCCGACCCTTTTGGACGGTGACGAAAAATTCAAGGTTCATATTTACATGTGGCACGGCTTTTCGACTGAGCTCTATATGA
CGATCGGCGTCGTTGCTCTCGGAGTTCTCGCCTATTTGACCTTGTCAAAATGGCGGAAGGTCTACGGCCTCTTCCCGGAA
ACACTGACGCTGAACAAGTTGTACGACAGCTCGCTGTCAGGGCTTGAAAAAGGCTCCAACAAGATTACAAGAACGTATAT
GACAGGGTTTCTCCGCGACTATCTTGTTTATGTTTTTGTCTTCTTTATCGTGATCATCGGCGGATCGCTCGCTGTTACCG
GGGGCTTTTCATACGATGTGAAAGGCAATGCTTCAATCGGCGTCTATGAGCTCGTTCTGTCAGCCGTAATGATTGCCGCA
ACCTTTACCACCGTTTTTGCAAGGTCAAGACTGACGGCGATTATCGGACTCGGCGTCATCGGCTACACGCTCGCGCTGTT
CTTTGTCATTTTCAGGGCGCCGGATCTCGCGCTGACGCAGCTGATCATCGAAACCATTTCAGTCGCATTGTTCCTGCTGT
GCTTCTATCATTTGCCGAAGCTCAGATTTAAGCCGAAATCGGCTAGGTTCAGGGTCACAAATGCGCTTGTATCGGTCGGC
GTCGGAACTGTTGTGACGCTGCTCGCACTTTCAGCAAACAGCCAGCGCTCGCTTGAAAGCATTGCATCGTATTTTATTGA
AAACAGCTATAAACTCGCAGGCGGCCACAATATCGTCAACGTAATATTGGTCGACTTCAGGGGATTTGATACATTGTTTG
AAATTACCGTACTTGTCATTGCCGCTTTAGGAATATACGGCATGATCAGGCTCAGATTGGAGAAAGGAGGGGAAGAGCAT
TGA

Upstream 100 bases:

>100_bases
TAATTTGTCTAGGTCACCAGTTCTAAAAAAGTACATAACGTTTGAAAATTTTAGTATGGAACAGAATGTATTTATACATG
CTACCTTAAGGGAGGTTAAT

Downstream 100 bases:

>100_bases
AAAAGGTTCAGACGAATGATGTGATCCTGCAAACCGTTACAAAGATTGCATCCTTTATCATTCTGCTCTTTTCTTTCCAT
CTGTTTTTTGCCGGCCATAA

Product: monovalent cation/H+ antiporter subunit A

Products: NAD+; ubiquinol [C]

Alternate protein names: Mrp complex subunit A; Multiple resistance and pH homeostasis protein A [H]

Number of amino acids: Translated: 800; Mature: 799

Protein sequence:

>800_residues
MTSLHIAILSPFALAFLIPLLAKNVRKIHTGWFVLFLPVLLFIYFFRMIKMTADGGTLKAVLEWIPSFGINFTAYVDGLG
LLFALLITGIGSLVVLYSIYYLSKEREQLGPFYVYLLMFMGAMLGVVLVDNVIVLYLFWELTSISSFLLIGYWYKREKSR
YGATKSMLITIFGGLSMLGGFILLYTITGSFSIREMINQVDLISGHGFFIPALVLVLIGAFTKSAQFPFYIWLPDAMEAP
TPVSAYLHSATMVKAGIYIVARFTPVFAFSGEWFWIVSLTGIVTLFWGSFNAVKQTDLKSILAFSTVSQLGMIMSMLGVG
AAALHFQHFEYYMVAVIAAIFHLINHATFKGSLFMAVGIIDHETGTRDIRKLGGLMTIMPITFTITLIGAFSMAGLPPFN
GFLSKEMFFTSMLRITNFNLYNADTWGFLFPVLAWIGSVFTFIYCMVLLFKTFGGKYQPEKLEKQAHEAPIGMLISPIVL
VSLVVIFFFFPNILSYSIIEPAAASIMPTLLDGDEKFKVHIYMWHGFSTELYMTIGVVALGVLAYLTLSKWRKVYGLFPE
TLTLNKLYDSSLSGLEKGSNKITRTYMTGFLRDYLVYVFVFFIVIIGGSLAVTGGFSYDVKGNASIGVYELVLSAVMIAA
TFTTVFARSRLTAIIGLGVIGYTLALFFVIFRAPDLALTQLIIETISVALFLLCFYHLPKLRFKPKSARFRVTNALVSVG
VGTVVTLLALSANSQRSLESIASYFIENSYKLAGGHNIVNVILVDFRGFDTLFEITVLVIAALGIYGMIRLRLEKGGEEH

Sequences:

>Translated_800_residues
MTSLHIAILSPFALAFLIPLLAKNVRKIHTGWFVLFLPVLLFIYFFRMIKMTADGGTLKAVLEWIPSFGINFTAYVDGLG
LLFALLITGIGSLVVLYSIYYLSKEREQLGPFYVYLLMFMGAMLGVVLVDNVIVLYLFWELTSISSFLLIGYWYKREKSR
YGATKSMLITIFGGLSMLGGFILLYTITGSFSIREMINQVDLISGHGFFIPALVLVLIGAFTKSAQFPFYIWLPDAMEAP
TPVSAYLHSATMVKAGIYIVARFTPVFAFSGEWFWIVSLTGIVTLFWGSFNAVKQTDLKSILAFSTVSQLGMIMSMLGVG
AAALHFQHFEYYMVAVIAAIFHLINHATFKGSLFMAVGIIDHETGTRDIRKLGGLMTIMPITFTITLIGAFSMAGLPPFN
GFLSKEMFFTSMLRITNFNLYNADTWGFLFPVLAWIGSVFTFIYCMVLLFKTFGGKYQPEKLEKQAHEAPIGMLISPIVL
VSLVVIFFFFPNILSYSIIEPAAASIMPTLLDGDEKFKVHIYMWHGFSTELYMTIGVVALGVLAYLTLSKWRKVYGLFPE
TLTLNKLYDSSLSGLEKGSNKITRTYMTGFLRDYLVYVFVFFIVIIGGSLAVTGGFSYDVKGNASIGVYELVLSAVMIAA
TFTTVFARSRLTAIIGLGVIGYTLALFFVIFRAPDLALTQLIIETISVALFLLCFYHLPKLRFKPKSARFRVTNALVSVG
VGTVVTLLALSANSQRSLESIASYFIENSYKLAGGHNIVNVILVDFRGFDTLFEITVLVIAALGIYGMIRLRLEKGGEEH
>Mature_799_residues
TSLHIAILSPFALAFLIPLLAKNVRKIHTGWFVLFLPVLLFIYFFRMIKMTADGGTLKAVLEWIPSFGINFTAYVDGLGL
LFALLITGIGSLVVLYSIYYLSKEREQLGPFYVYLLMFMGAMLGVVLVDNVIVLYLFWELTSISSFLLIGYWYKREKSRY
GATKSMLITIFGGLSMLGGFILLYTITGSFSIREMINQVDLISGHGFFIPALVLVLIGAFTKSAQFPFYIWLPDAMEAPT
PVSAYLHSATMVKAGIYIVARFTPVFAFSGEWFWIVSLTGIVTLFWGSFNAVKQTDLKSILAFSTVSQLGMIMSMLGVGA
AALHFQHFEYYMVAVIAAIFHLINHATFKGSLFMAVGIIDHETGTRDIRKLGGLMTIMPITFTITLIGAFSMAGLPPFNG
FLSKEMFFTSMLRITNFNLYNADTWGFLFPVLAWIGSVFTFIYCMVLLFKTFGGKYQPEKLEKQAHEAPIGMLISPIVLV
SLVVIFFFFPNILSYSIIEPAAASIMPTLLDGDEKFKVHIYMWHGFSTELYMTIGVVALGVLAYLTLSKWRKVYGLFPET
LTLNKLYDSSLSGLEKGSNKITRTYMTGFLRDYLVYVFVFFIVIIGGSLAVTGGFSYDVKGNASIGVYELVLSAVMIAAT
FTTVFARSRLTAIIGLGVIGYTLALFFVIFRAPDLALTQLIIETISVALFLLCFYHLPKLRFKPKSARFRVTNALVSVGV
GTVVTLLALSANSQRSLESIASYFIENSYKLAGGHNIVNVILVDFRGFDTLFEITVLVIAALGIYGMIRLRLEKGGEEH

Specific function: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual N

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=386, Percent_Identity=33.9378238341969, Blast_Score=179, Evalue=1e-44,
Organism=Escherichia coli, GI1788614, Length=496, Percent_Identity=32.4596774193548, Blast_Score=216, Evalue=5e-57,
Organism=Escherichia coli, GI1788829, Length=431, Percent_Identity=33.1786542923434, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1788831, Length=393, Percent_Identity=31.8066157760814, Blast_Score=147, Evalue=3e-36,
Organism=Escherichia coli, GI1788827, Length=449, Percent_Identity=28.2850779510022, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI1788613, Length=427, Percent_Identity=28.1030444964871, Blast_Score=123, Evalue=4e-29,
Organism=Escherichia coli, GI2367154, Length=260, Percent_Identity=27.6923076923077, Blast_Score=106, Evalue=6e-24,
Organism=Escherichia coli, GI145693160, Length=351, Percent_Identity=26.7806267806268, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005663
- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 88981; Mature: 88850

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TSLHIAILSPFALAFLIPLLAKNVRKIHTGWFVLFLPVLLFIYFFRMIKMTADGGTLKA
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
VLEWIPSFGINFTAYVDGLGLLFALLITGIGSLVVLYSIYYLSKEREQLGPFYVYLLMFM
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAMLGVVLVDNVIVLYLFWELTSISSFLLIGYWYKREKSRYGATKSMLITIFGGLSMLGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
FILLYTITGSFSIREMINQVDLISGHGFFIPALVLVLIGAFTKSAQFPFYIWLPDAMEAP
HHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCC
TPVSAYLHSATMVKAGIYIVARFTPVFAFSGEWFWIVSLTGIVTLFWGSFNAVKQTDLKS
CCHHHHHHHHHHHHHCEEEEEEECEEEEECCCEEEEEHHHHHHHHHHCCCCHHHHHHHHH
ILAFSTVSQLGMIMSMLGVGAAALHFQHFEYYMVAVIAAIFHLINHATFKGSLFMAVGII
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEE
DHETGTRDIRKLGGLMTIMPITFTITLIGAFSMAGLPPFNGFLSKEMFFTSMLRITNFNL
ECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCE
YNADTWGFLFPVLAWIGSVFTFIYCMVLLFKTFGGKYQPEKLEKQAHEAPIGMLISPIVL
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHH
VSLVVIFFFFPNILSYSIIEPAAASIMPTLLDGDEKFKVHIYMWHGFSTELYMTIGVVAL
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEEEEEECCCHHHHHHHHHHHH
GVLAYLTLSKWRKVYGLFPETLTLNKLYDSSLSGLEKGSNKITRTYMTGFLRDYLVYVFV
HHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
FFIVIIGGSLAVTGGFSYDVKGNASIGVYELVLSAVMIAATFTTVFARSRLTAIIGLGVI
HHHHHHCCCEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYTLALFFVIFRAPDLALTQLIIETISVALFLLCFYHLPKLRFKPKSARFRVTNALVSVG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
VGTVVTLLALSANSQRSLESIASYFIENSYKLAGGHNIVNVILVDFRGFDTLFEITVLVI
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEECCCCCEEEEEEEECCCHHHHHHHHHHHH
AALGIYGMIRLRLEKGGEEH
HHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 10198001; 11356194 [H]