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The map label for this gene is adaB [H]

Identifier: 52786571

GI number: 52786571

Start: 2723140

End: 2723667

Strand: Direct

Name: adaB [H]

Synonym: BLi02837

Alternate gene names: 52786571

Gene position: 2723140-2723667 (Clockwise)

Preceding gene: 52786570

Following gene: 52786574

Centisome position: 64.49

GC content: 53.41

Gene sequence:

>528_bases
ATGGACGATCAAATCGTATACTGGCGTACGCTTATTTGCCGAGGTTGGCAGATTCACATTGGGGCGACTGCCCGCGGGCT
CTGTTTTACAGGGGGATGGAATCAAGGCTTCGAGGATTTGGCCGCTTGGGCTGAAAAAAGATTTACACAGCCAGTTTTCA
TTCGGGATGACAAAGGATTGGCAGAATACGCCGAGCAGCTGCAGGCGTATTTGAACGGCAAGCGAACTCATTTCAGCTTT
CCTGTCGACCTTGCCGGCACGCCTTTTCAGCTGGCAGTGTGGAAGGCGCTCTCCGAAATCCCTTACGGCAGCACTTGCTC
CTATTCCGATATCGCCGAGCATATTGAAAAGCAGGCCGCTGTGCGGGCAGTAGGCGCGGCGATCGGCGCAAATCCGCTGT
TAATGGTGGTGCCTTGCCATCGGGTTATCGGTAAAAGCGGGCAGCTCACGGGATACCGGGGCGGATTGGATATGAAAAAA
GAACTGATGATGATGGAGTCCGGCGGTGTAACCGCACAATTCAATTAA

Upstream 100 bases:

>100_bases
ATGGTAGGCATTGCGAATGCGGAATATTTTGCCTCTTTGTTTAAAAAGAAGACTGGGCAATCGCCGACGTCGTACCGGCA
AATGAAACGGAGGTAAACAT

Downstream 100 bases:

>100_bases
AGCCCCGTTCGAATGTCGAACGGGGCCGTTTTGTTAAAAGTCAAAGTTATCGGGATCAGGGCCGACACGGGTGTTTTCAT
TCAGCGCGTCAATTTGCTTC

Product: AdaB

Products: NA

Alternate protein names: O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MDDQIVYWRTLICRGWQIHIGATARGLCFTGGWNQGFEDLAAWAEKRFTQPVFIRDDKGLAEYAEQLQAYLNGKRTHFSF
PVDLAGTPFQLAVWKALSEIPYGSTCSYSDIAEHIEKQAAVRAVGAAIGANPLLMVVPCHRVIGKSGQLTGYRGGLDMKK
ELMMMESGGVTAQFN

Sequences:

>Translated_175_residues
MDDQIVYWRTLICRGWQIHIGATARGLCFTGGWNQGFEDLAAWAEKRFTQPVFIRDDKGLAEYAEQLQAYLNGKRTHFSF
PVDLAGTPFQLAVWKALSEIPYGSTCSYSDIAEHIEKQAAVRAVGAAIGANPLLMVVPCHRVIGKSGQLTGYRGGLDMKK
ELMMMESGGVTAQFN
>Mature_175_residues
MDDQIVYWRTLICRGWQIHIGATARGLCFTGGWNQGFEDLAAWAEKRFTQPVFIRDDKGLAEYAEQLQAYLNGKRTHFSF
PVDLAGTPFQLAVWKALSEIPYGSTCSYSDIAEHIEKQAAVRAVGAAIGANPLLMVVPCHRVIGKSGQLTGYRGGLDMKK
ELMMMESGGVTAQFN

Specific function: Repair of alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction:the enzyme is irreversibly inactivated [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=111, Percent_Identity=42.3423423423423, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI1787596, Length=104, Percent_Identity=45.1923076923077, Blast_Score=96, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=50, Blast_Score=83, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=50, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320001, Length=84, Percent_Identity=42.8571428571429, Blast_Score=80, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17137554, Length=125, Percent_Identity=33.6, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19349; Mature: 19349

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDQIVYWRTLICRGWQIHIGATARGLCFTGGWNQGFEDLAAWAEKRFTQPVFIRDDKGL
CCCCEEHHHHHHHCCCEEEECCCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCH
AEYAEQLQAYLNGKRTHFSFPVDLAGTPFQLAVWKALSEIPYGSTCSYSDIAEHIEKQAA
HHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
VRAVGAAIGANPLLMVVPCHRVIGKSGQLTGYRGGLDMKKELMMMESGGVTAQFN
HHHHHHHHCCCCEEEEEECHHHHCCCCCCCEECCCCCHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure
MDDQIVYWRTLICRGWQIHIGATARGLCFTGGWNQGFEDLAAWAEKRFTQPVFIRDDKGL
CCCCEEHHHHHHHCCCEEEECCCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCH
AEYAEQLQAYLNGKRTHFSFPVDLAGTPFQLAVWKALSEIPYGSTCSYSDIAEHIEKQAA
HHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
VRAVGAAIGANPLLMVVPCHRVIGKSGQLTGYRGGLDMKKELMMMESGGVTAQFN
HHHHHHHHCCCCEEEEEECHHHHCCCCCCCEECCCCCHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2120677; 9384377; 1744039 [H]