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The map label for this gene is adaA [H]

Identifier: 52786570

GI number: 52786570

Start: 2722575

End: 2723135

Strand: Direct

Name: adaA [H]

Synonym: BLi02836

Alternate gene names: 52786570

Gene position: 2722575-2723135 (Clockwise)

Preceding gene: 52786568

Following gene: 52786571

Centisome position: 64.48

GC content: 49.73

Gene sequence:

>561_bases
ATGAACAGCAAATCACGATTGACGCCTGAAATGTGGCGGGCGATTACGGAAAACGATTCCGCCTATGACGGAGTTTTTTA
TTACGCGGTCAAAACGACCGGCATATTTTGCCGCCCTTCCTGCAAATCGAGAGTTCCGCAAATCGACAATGTGCAGATCT
TTTTCAATGCAAAAGATGCTTTATCAGAAGGGTACCGCCCCTGCAAACGCTGCAATCCGGCCGGGGCGCTGCTGCCGGAT
GAAGAGCTGGCACAGCGTGTGGTGGAAATCATCGAGGAATCTTATCGCGATCCGCTGTCTCTGCAAGCTTTGGCTGACAG
GTGCCATATCAGCCCTTTTCACCTGCAGCGGACATTTAAACGAATCAAAGGCGTCTCGCCGGCAGAATACATCCTGCAGA
AAAGGATTGAGAAAGCGGTCGATTTGCTTGCTCATTCAGAACGATCGATCGCAGAAATCGCTTCAATGGTAGGCATTGCG
AATGCGGAATATTTTGCCTCTTTGTTTAAAAAGAAGACTGGGCAATCGCCGACGTCGTACCGGCAAATGAAACGGAGGTA
A

Upstream 100 bases:

>100_bases
CAACAGTCTCTCTTCTTCTTTGTCTTCTTGCTGTGCCGTTCTCCGCCTCTCCACCTCCGGCTGTCATATAGGTTATACTG
ACATAGATCGGGGTGAAGAT

Downstream 100 bases:

>100_bases
ACATATGGACGATCAAATCGTATACTGGCGTACGCTTATTTGCCGAGGTTGGCAGATTCACATTGGGGCGACTGCCCGCG
GGCTCTGTTTTACAGGGGGA

Product: AdaA

Products: DNA lacking O-methylguanine; protein S-methyl-L-cysteine [C]

Alternate protein names: NA

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MNSKSRLTPEMWRAITENDSAYDGVFYYAVKTTGIFCRPSCKSRVPQIDNVQIFFNAKDALSEGYRPCKRCNPAGALLPD
EELAQRVVEIIEESYRDPLSLQALADRCHISPFHLQRTFKRIKGVSPAEYILQKRIEKAVDLLAHSERSIAEIASMVGIA
NAEYFASLFKKKTGQSPTSYRQMKRR

Sequences:

>Translated_186_residues
MNSKSRLTPEMWRAITENDSAYDGVFYYAVKTTGIFCRPSCKSRVPQIDNVQIFFNAKDALSEGYRPCKRCNPAGALLPD
EELAQRVVEIIEESYRDPLSLQALADRCHISPFHLQRTFKRIKGVSPAEYILQKRIEKAVDLLAHSERSIAEIASMVGIA
NAEYFASLFKKKTGQSPTSYRQMKRR
>Mature_186_residues
MNSKSRLTPEMWRAITENDSAYDGVFYYAVKTTGIFCRPSCKSRVPQIDNVQIFFNAKDALSEGYRPCKRCNPAGALLPD
EELAQRVVEIIEESYRDPLSLQALADRCHISPFHLQRTFKRIKGVSPAEYILQKRIEKAVDLLAHSERSIAEIASMVGIA
NAEYFASLFKKKTGQSPTSYRQMKRR

Specific function: Methylphosphotriester-DNA alkyltransferase is one of two genes required for the adaptive response of bacteria to alkylating agents. It accepts a methyl group from methylphospho-triesters and then acts as a transcriptional activator of the ada operon [H]

COG id: COG2169

COG function: function code F; Adenosine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=146, Percent_Identity=38.3561643835616, Blast_Score=110, Evalue=6e-26,
Organism=Escherichia coli, GI1790339, Length=96, Percent_Identity=32.2916666666667, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1790391, Length=102, Percent_Identity=30.3921568627451, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1787987, Length=100, Percent_Identity=31, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR016220 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF00165 HTH_AraC [H]

EC number: 2.1.1.63 [C]

Molecular weight: Translated: 21199; Mature: 21199

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSKSRLTPEMWRAITENDSAYDGVFYYAVKTTGIFCRPSCKSRVPQIDNVQIFFNAKDA
CCCCCCCCHHHHHHHHCCCCCCCCEEEEEEECCCEEECCCHHHCCCCCCCEEEEECCHHH
LSEGYRPCKRCNPAGALLPDEELAQRVVEIIEESYRDPLSLQALADRCHISPFHLQRTFK
HHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHH
RIKGVSPAEYILQKRIEKAVDLLAHSERSIAEIASMVGIANAEYFASLFKKKTGQSPTSY
HHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHH
RQMKRR
HHHHCC
>Mature Secondary Structure
MNSKSRLTPEMWRAITENDSAYDGVFYYAVKTTGIFCRPSCKSRVPQIDNVQIFFNAKDA
CCCCCCCCHHHHHHHHCCCCCCCCEEEEEEECCCEEECCCHHHCCCCCCCEEEEECCHHH
LSEGYRPCKRCNPAGALLPDEELAQRVVEIIEESYRDPLSLQALADRCHISPFHLQRTFK
HHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHH
RIKGVSPAEYILQKRIEKAVDLLAHSERSIAEIASMVGIANAEYFASLFKKKTGQSPTSY
HHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHH
RQMKRR
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA containing O-methylguanine; protein L-cysteine; DNA [C]

Specific reaction: DNA containing O-methylguanine + protein L-cysteine = DNA lacking O-methylguanine + protein S-methyl-L-cysteine Protein + DNA = Protein-DNA [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2120677; 9384377; 1744039 [H]