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The map label for this gene is gid

Identifier: 52785592

GI number: 52785592

Start: 1791049

End: 1792353

Strand: Direct

Name: gid

Synonym: BLi01833

Alternate gene names: 52785592

Gene position: 1791049-1792353 (Clockwise)

Preceding gene: 52785591

Following gene: 52785593

Centisome position: 42.42

GC content: 48.28

Gene sequence:

>1305_bases
ATGAATCAAACAGTAAATGTCATCGGTGCAGGACTTGCAGGAAGCGAAGCGGCATGGCAGCTTGCTAAGCGCGGTATCAA
TGTCCGTTTATACGAAATGAGGCCAGTCAAGCAAACGCCGGCGCACCATACAGATAAATTTGCCGAACTTGTCTGCAGCA
ACTCGCTTCGGGCGAATTCATTAACGAATGCTGTCGGTGTATTAAAGGAAGAAATGCGGGTTCTTGACTCGGCCATCATC
GCTGCTGCTGACGAATGTTCTGTTCCTGCCGGCGGTGCGCTTGCCGTAGACAGGCATGAATTTGCCGCAAACGTTACCGA
AAAAGTAAAGAATCATCCGAATGTGACCGTGCTTCATGAAGAAGTCACGGAAATACCGGAAGGACCGACAATCATTGCGA
CAGGACCGCTGACATCAGAAGCGCTTTCCGCAAAGCTCCGGGAGCTGACAGGCGAGGACTATCTATACTTTTATGACGCG
GCTGCGCCGATTGTTGAAAAAGACAGCCTTGACATGGATAAAGTCTATTTGAAATCCCGCTATGACAAAGGGGAGGCAGC
GTATTTGAACTGTCCGATGACAGAAGAAGAGTTTGACAGGTTTTATGACGCCCTTGTGTCTGCGGAAACCGTTCCATTGA
AAGAATTTGAAAAGGAAATCTTCTTTGAAGGCTGTATGCCGATTGAAGTCATGGCGAAGCGCGGCAAAAAAACCATGCTG
TTCGGCCCGATGAAGCCTGTCGGACTTGAAGACCCAAAAACAGGAAAGCGTCCGTACGCCGTTGTGCAGCTGCGCCAAGA
TGATGCAGCCGGCACGCTTTACAATATCGTCGGTTTTCAAACCCACCTCAAATGGGGCGACCAAAAAGAAGTGCTCCGCC
TCATTCCGGGACTGGAACAGGCAGAAATCGTCCGCTACGGCGTGATGCACAGAAATACGTTCATCAACTCGCCGAGTCTG
TTAAAAGCGACCTACCAGTTTAAAAAGCGGGACGATCTGTTTTTTGCCGGTCAAATGACAGGTGTTGAAGGGTATGTTGA
ATCAGCGGCTTCAGGTCTTGTTGCCGGTATAAATGCGGCTCGGCTCATCCAGGGAAAAGAACCTGTCACTTTTTCAAATG
AAACAGCGATCGGCAGCATGGCCCATTACATTACGGAAACAAACAAGAAAAACTTCCAGCCGATGAATGCAAACTTCGGC
CTGTTCAAAGAATTGGGTGTTAAAATTAAAAACAAGCAGGAACGAAATGAACAGTATGCTTCGCGCGCATTGGAGACAAT
TCGAAATATTTCGAAAACGCTATAG

Upstream 100 bases:

>100_bases
TTATAAGGAAGAACAACAAAAGTAGCGGTGAGCAGGGCTTTGCTCACCTTCTTTGTTGTGTTAAAAGAATAAGTCAATTC
ATAAATTAGGAGATGTGAAT

Downstream 100 bases:

>100_bases
TTATTCATTGCCAGATGAACTTGTTATGTGATAACATTTAAAAGCCCTTTTCGAAGAGGTGTAATGATGGCAAATGTTAA
GAATTTTTTAACTTTATTCA

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MNQTVNVIGAGLAGSEAAWQLAKRGINVRLYEMRPVKQTPAHHTDKFAELVCSNSLRANSLTNAVGVLKEEMRVLDSAII
AAADECSVPAGGALAVDRHEFAANVTEKVKNHPNVTVLHEEVTEIPEGPTIIATGPLTSEALSAKLRELTGEDYLYFYDA
AAPIVEKDSLDMDKVYLKSRYDKGEAAYLNCPMTEEEFDRFYDALVSAETVPLKEFEKEIFFEGCMPIEVMAKRGKKTML
FGPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNIVGFQTHLKWGDQKEVLRLIPGLEQAEIVRYGVMHRNTFINSPSL
LKATYQFKKRDDLFFAGQMTGVEGYVESAASGLVAGINAARLIQGKEPVTFSNETAIGSMAHYITETNKKNFQPMNANFG
LFKELGVKIKNKQERNEQYASRALETIRNISKTL

Sequences:

>Translated_434_residues
MNQTVNVIGAGLAGSEAAWQLAKRGINVRLYEMRPVKQTPAHHTDKFAELVCSNSLRANSLTNAVGVLKEEMRVLDSAII
AAADECSVPAGGALAVDRHEFAANVTEKVKNHPNVTVLHEEVTEIPEGPTIIATGPLTSEALSAKLRELTGEDYLYFYDA
AAPIVEKDSLDMDKVYLKSRYDKGEAAYLNCPMTEEEFDRFYDALVSAETVPLKEFEKEIFFEGCMPIEVMAKRGKKTML
FGPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNIVGFQTHLKWGDQKEVLRLIPGLEQAEIVRYGVMHRNTFINSPSL
LKATYQFKKRDDLFFAGQMTGVEGYVESAASGLVAGINAARLIQGKEPVTFSNETAIGSMAHYITETNKKNFQPMNANFG
LFKELGVKIKNKQERNEQYASRALETIRNISKTL
>Mature_434_residues
MNQTVNVIGAGLAGSEAAWQLAKRGINVRLYEMRPVKQTPAHHTDKFAELVCSNSLRANSLTNAVGVLKEEMRVLDSAII
AAADECSVPAGGALAVDRHEFAANVTEKVKNHPNVTVLHEEVTEIPEGPTIIATGPLTSEALSAKLRELTGEDYLYFYDA
AAPIVEKDSLDMDKVYLKSRYDKGEAAYLNCPMTEEEFDRFYDALVSAETVPLKEFEKEIFFEGCMPIEVMAKRGKKTML
FGPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNIVGFQTHLKWGDQKEVLRLIPGLEQAEIVRYGVMHRNTFINSPSL
LKATYQFKKRDDLFFAGQMTGVEGYVESAASGLVAGINAARLIQGKEPVTFSNETAIGSMAHYITETNKKNFQPMNANFG
LFKELGVKIKNKQERNEQYASRALETIRNISKTL

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily

Homologues:

Organism=Escherichia coli, GI2367273, Length=90, Percent_Identity=45.5555555555556, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17534255, Length=135, Percent_Identity=37.7777777777778, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMFO_BACLD (Q65JN6)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_079006.1
- RefSeq:   YP_091421.1
- ProteinModelPortal:   Q65JN6
- SMR:   Q65JN6
- STRING:   Q65JN6
- EnsemblBacteria:   EBBACT00000056304
- EnsemblBacteria:   EBBACT00000062235
- GeneID:   3031005
- GeneID:   3098735
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi01833
- KEGG:   bli:BL01280
- NMPDR:   fig|279010.5.peg.2536
- eggNOG:   COG1206
- GeneTree:   EBGT00050000001476
- HOGENOM:   HBG285041
- OMA:   FEGCLPI
- ProtClustDB:   PRK05335
- BioCyc:   BLIC279010-1:BLI01833-MONOMER
- BioCyc:   BLIC279010:BL01280-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01037
- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00137

Pfam domain/function: PF01134 GIDA

EC number: =2.1.1.74

Molecular weight: Translated: 48114; Mature: 48114

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQTVNVIGAGLAGSEAAWQLAKRGINVRLYEMRPVKQTPAHHTDKFAELVCSNSLRANS
CCCCEEEEECCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCHHH
LTNAVGVLKEEMRVLDSAIIAAADECSVPAGGALAVDRHEFAANVTEKVKNHPNVTVLHE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCEEEEEH
EVTEIPEGPTIIATGPLTSEALSAKLRELTGEDYLYFYDAAAPIVEKDSLDMDKVYLKSR
HHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHC
YDKGEAAYLNCPMTEEEFDRFYDALVSAETVPLKEFEKEIFFEGCMPIEVMAKRGKKTML
CCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHCCCEEEE
FGPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNIVGFQTHLKWGDQKEVLRLIPGLEQ
ECCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCH
AEIVRYGVMHRNTFINSPSLLKATYQFKKRDDLFFAGQMTGVEGYVESAASGLVAGINAA
HHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCHH
RLIQGKEPVTFSNETAIGSMAHYITETNKKNFQPMNANFGLFKELGVKIKNKQERNEQYA
HHCCCCCCCEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCEECCHHHHHHHHH
SRALETIRNISKTL
HHHHHHHHHHHHCC
>Mature Secondary Structure
MNQTVNVIGAGLAGSEAAWQLAKRGINVRLYEMRPVKQTPAHHTDKFAELVCSNSLRANS
CCCCEEEEECCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCHHH
LTNAVGVLKEEMRVLDSAIIAAADECSVPAGGALAVDRHEFAANVTEKVKNHPNVTVLHE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCEEEEEH
EVTEIPEGPTIIATGPLTSEALSAKLRELTGEDYLYFYDAAAPIVEKDSLDMDKVYLKSR
HHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHC
YDKGEAAYLNCPMTEEEFDRFYDALVSAETVPLKEFEKEIFFEGCMPIEVMAKRGKKTML
CCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHCCCEEEE
FGPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNIVGFQTHLKWGDQKEVLRLIPGLEQ
ECCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCH
AEIVRYGVMHRNTFINSPSLLKATYQFKKRDDLFFAGQMTGVEGYVESAASGLVAGINAA
HHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCHH
RLIQGKEPVTFSNETAIGSMAHYITETNKKNFQPMNANFGLFKELGVKIKNKQERNEQYA
HHCCCCCCCEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCEECCHHHHHHHHH
SRALETIRNISKTL
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA