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The map label for this gene is topA [H]

Identifier: 52785591

GI number: 52785591

Start: 1788898

End: 1790973

Strand: Direct

Name: topA [H]

Synonym: BLi01832

Alternate gene names: 52785591

Gene position: 1788898-1790973 (Clockwise)

Preceding gene: 52785590

Following gene: 52785592

Centisome position: 42.36

GC content: 47.45

Gene sequence:

>2076_bases
ATGTCCGAATACTTAGTCATCGTGGAATCGCCTGCAAAGGCAAAAACGATTGAGCGATACTTAGGAAAAAAATATAAAGT
AAAAGCCTCGATGGGGCACGTCAGAGATTTGCCGAAAAGCCAGATGGGCGTTGACATCGAGCAGAACTTTGAACCAAAAT
ACATTACGATCCGCGGGAAAGGCCCCGTGTTGAAAGAATTAAAAACGGCCGCCAAAAAGGCAAAAAAAGTTTATCTCGCA
GCCGACCCCGACAGGGAAGGGGAAGCGATTGCGTGGCATTTGGCGCACAGCCTTGATTTGGACCTGAATTCCGACTGCCG
CGTCGTATTTAATGAAATTACAAAAGATGCGATCAAAGAATCATTTAAGCATCCGCGCATGATCAATATGGATCTTGTCG
ACGCCCAGCAGGCGAGAAGAATTCTGGACCGCCTTGTAGGGTACAAAATCAGTCCGATATTATGGAAAAAAGTAAAAAAA
GGCTTGAGTGCGGGACGTGTCCAATCTGTTGCGCTCCGCCTTATCATTGACCGGGAGAAAGAAATCAACGATTTTATACC
GGAAGAATATTGGACAATTGAAGGGTCTTTTCTCAAAGGTAAAGAAACGTTTGAAGCGAGCTTCTTCGGAATCAACGGGG
AAAAGCACCAGCTGAAAAGCGAAGATGATGTGAAGCAAATCCTATCCCGGATCAAAGGGAACAAATTTGAGGTCAAAAAG
GTAACGAAAAAAGAACGGAAGCGAAATCCTGCGCTCCCATTTACGACGTCGACGCTTCAGCAGGAAGCGGCGCGCAAATT
GAATTTCCGGGCAAAGAAAACGATGATGATCGCCCAGCAGCTTTATGAAGGAATCGATCTCGGCAAAGAAGGAACGGTCG
GGTTAATCACTTATATGAGAACGGATTCAACCCGTATCTCGAATACTGCTCAAGAAGAAGCGGCATCATTCATCGGCGAA
CAGTACGGAAAAGAATTTTTGGGTAGCAAGCGAAAGCCGGCCAAAAAGAATGAAAACGCCCAGGATGCCCACGAAGCGAT
CAGGCCGACCTCGGTGCTCAGAAAGCCTTCCGATCTGAAGGCGGTATTGGGAAGAGACCAGCTTCGGCTATACAAGCTGA
TCTGGGAGCGCTTTGTCGCAAGCCAAATGGCTCCGGCGACACTGGATACGATGAGCGTCGACCTTGAAAACAACGGGTTG
ACATTTCGGGCAAACGGCAGTAAAGTCAAGTTTTCAGGCTTCATGAAAGTGTACGTTGAAGGCAAAGACGATCAGATGGA
AGAGAAGAATAAGATGCTTCCGGATCTCGCTGAAGGCGATACCGTGCTTTCAAAAGACATCGAGCCCGAACAGCACTTCA
CACAGCCGCCTCCGCGCTATACGGAAGCAAGGCTGGTCAAAACGCTTGAAGAACTGGGAATCGGACGTCCATCCACATAC
GCGCCGACGCTTGACACGATCCAAAAGCGCGGGTATGTCGCCCTTGATAATAAACGCTTTATTCCGACTGAGCTCGGTGA
AATTGTGCTTGACTTGATCATGGAGTTTTTCCCTGAGATTATTAACGTTGAGTTCACCGCCAAAATGGAAAAAGAGCTTG
ATGACGTTGAAGACGGAAACATTCAATGGGTGCAGATCATCGATTCCTTTTACAAGGACTTTGAAAAGCGTGTTGAAAAA
GCGGAAGCCGAGATGCAGGAAGTGGAGATCGAACCGGAGTATGCAGGTGTTGACTGTGAAGCGTGCGGACATCCGATGGT
CTATAAAATGGGCCGGTACGGCAAATTTATGGCCTGCTCCAATTTTCCGGACTGCCGCAACACCAAGCCGATCGTAAAAG
AGATCGGCGTCAAATGCCCATCATGCAAAACCGGCAACATCGTCGAGCGCAAATCGAAAAAACGCCGCATTTTTTACGGC
TGTGACCGCTATCCTGAATGTGAATTCGTATCATGGGATAAACCGCTTGAACGGAAATGTCCGAAATGCGAGGACATGCT
TGTGGAGAAAAAGCTCAAAAAAGGCGTTCAAGTGCAGTGCGTTAATTGCGATTATAAGGAAGAACAACAAAAGTAG

Upstream 100 bases:

>100_bases
TTTTCGTAAAAATAATGACTATATAAACGACGATTATGCTGAATACAATAAAAAGGCTAAATTGAAAAGAGAGAGAACAC
CTCTTGAGGGGGATAATGTT

Downstream 100 bases:

>100_bases
CGGTGAGCAGGGCTTTGCTCACCTTCTTTGTTGTGTTAAAAGAATAAGTCAATTCATAAATTAGGAGATGTGAATATGAA
TCAAACAGTAAATGTCATCG

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 691; Mature: 690

Protein sequence:

>691_residues
MSEYLVIVESPAKAKTIERYLGKKYKVKASMGHVRDLPKSQMGVDIEQNFEPKYITIRGKGPVLKELKTAAKKAKKVYLA
ADPDREGEAIAWHLAHSLDLDLNSDCRVVFNEITKDAIKESFKHPRMINMDLVDAQQARRILDRLVGYKISPILWKKVKK
GLSAGRVQSVALRLIIDREKEINDFIPEEYWTIEGSFLKGKETFEASFFGINGEKHQLKSEDDVKQILSRIKGNKFEVKK
VTKKERKRNPALPFTTSTLQQEAARKLNFRAKKTMMIAQQLYEGIDLGKEGTVGLITYMRTDSTRISNTAQEEAASFIGE
QYGKEFLGSKRKPAKKNENAQDAHEAIRPTSVLRKPSDLKAVLGRDQLRLYKLIWERFVASQMAPATLDTMSVDLENNGL
TFRANGSKVKFSGFMKVYVEGKDDQMEEKNKMLPDLAEGDTVLSKDIEPEQHFTQPPPRYTEARLVKTLEELGIGRPSTY
APTLDTIQKRGYVALDNKRFIPTELGEIVLDLIMEFFPEIINVEFTAKMEKELDDVEDGNIQWVQIIDSFYKDFEKRVEK
AEAEMQEVEIEPEYAGVDCEACGHPMVYKMGRYGKFMACSNFPDCRNTKPIVKEIGVKCPSCKTGNIVERKSKKRRIFYG
CDRYPECEFVSWDKPLERKCPKCEDMLVEKKLKKGVQVQCVNCDYKEEQQK

Sequences:

>Translated_691_residues
MSEYLVIVESPAKAKTIERYLGKKYKVKASMGHVRDLPKSQMGVDIEQNFEPKYITIRGKGPVLKELKTAAKKAKKVYLA
ADPDREGEAIAWHLAHSLDLDLNSDCRVVFNEITKDAIKESFKHPRMINMDLVDAQQARRILDRLVGYKISPILWKKVKK
GLSAGRVQSVALRLIIDREKEINDFIPEEYWTIEGSFLKGKETFEASFFGINGEKHQLKSEDDVKQILSRIKGNKFEVKK
VTKKERKRNPALPFTTSTLQQEAARKLNFRAKKTMMIAQQLYEGIDLGKEGTVGLITYMRTDSTRISNTAQEEAASFIGE
QYGKEFLGSKRKPAKKNENAQDAHEAIRPTSVLRKPSDLKAVLGRDQLRLYKLIWERFVASQMAPATLDTMSVDLENNGL
TFRANGSKVKFSGFMKVYVEGKDDQMEEKNKMLPDLAEGDTVLSKDIEPEQHFTQPPPRYTEARLVKTLEELGIGRPSTY
APTLDTIQKRGYVALDNKRFIPTELGEIVLDLIMEFFPEIINVEFTAKMEKELDDVEDGNIQWVQIIDSFYKDFEKRVEK
AEAEMQEVEIEPEYAGVDCEACGHPMVYKMGRYGKFMACSNFPDCRNTKPIVKEIGVKCPSCKTGNIVERKSKKRRIFYG
CDRYPECEFVSWDKPLERKCPKCEDMLVEKKLKKGVQVQCVNCDYKEEQQK
>Mature_690_residues
SEYLVIVESPAKAKTIERYLGKKYKVKASMGHVRDLPKSQMGVDIEQNFEPKYITIRGKGPVLKELKTAAKKAKKVYLAA
DPDREGEAIAWHLAHSLDLDLNSDCRVVFNEITKDAIKESFKHPRMINMDLVDAQQARRILDRLVGYKISPILWKKVKKG
LSAGRVQSVALRLIIDREKEINDFIPEEYWTIEGSFLKGKETFEASFFGINGEKHQLKSEDDVKQILSRIKGNKFEVKKV
TKKERKRNPALPFTTSTLQQEAARKLNFRAKKTMMIAQQLYEGIDLGKEGTVGLITYMRTDSTRISNTAQEEAASFIGEQ
YGKEFLGSKRKPAKKNENAQDAHEAIRPTSVLRKPSDLKAVLGRDQLRLYKLIWERFVASQMAPATLDTMSVDLENNGLT
FRANGSKVKFSGFMKVYVEGKDDQMEEKNKMLPDLAEGDTVLSKDIEPEQHFTQPPPRYTEARLVKTLEELGIGRPSTYA
PTLDTIQKRGYVALDNKRFIPTELGEIVLDLIMEFFPEIINVEFTAKMEKELDDVEDGNIQWVQIIDSFYKDFEKRVEKA
EAEMQEVEIEPEYAGVDCEACGHPMVYKMGRYGKFMACSNFPDCRNTKPIVKEIGVKCPSCKTGNIVERKSKKRRIFYGC
DRYPECEFVSWDKPLERKCPKCEDMLVEKKLKKGVQVQCVNCDYKEEQQK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=624, Percent_Identity=24.5192307692308, Blast_Score=128, Evalue=2e-29,
Organism=Homo sapiens, GI4507635, Length=570, Percent_Identity=22.8070175438596, Blast_Score=108, Evalue=2e-23,
Organism=Escherichia coli, GI1787529, Length=748, Percent_Identity=38.6363636363636, Blast_Score=506, Evalue=1e-144,
Organism=Escherichia coli, GI48994931, Length=110, Percent_Identity=42.7272727272727, Blast_Score=110, Evalue=4e-25,
Organism=Escherichia coli, GI1788061, Length=540, Percent_Identity=22.4074074074074, Blast_Score=100, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17555378, Length=505, Percent_Identity=24.950495049505, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI32563869, Length=564, Percent_Identity=24.822695035461, Blast_Score=123, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6323263, Length=562, Percent_Identity=23.3096085409253, Blast_Score=107, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24585251, Length=630, Percent_Identity=23.968253968254, Blast_Score=129, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24640096, Length=543, Percent_Identity=24.3093922651934, Blast_Score=109, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 79175; Mature: 79044

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEYLVIVESPAKAKTIERYLGKKYKVKASMGHVRDLPKSQMGVDIEQNFEPKYITIRGK
CCCEEEEEECCCHHHHHHHHCCCCEEEEHHHHHHHCCCHHHHCCCHHCCCCCCEEEEECC
GPVLKELKTAAKKAKKVYLAADPDREGEAIAWHLAHSLDLDLNSDCRVVFNEITKDAIKE
CCHHHHHHHHHHHHHEEEEEECCCCCCCEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHH
SFKHPRMINMDLVDAQQARRILDRLVGYKISPILWKKVKKGLSAGRVQSVALRLIIDREK
HHCCCCEEEEHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCH
EINDFIPEEYWTIEGSFLKGKETFEASFFGINGEKHQLKSEDDVKQILSRIKGNKFEVKK
HHHHCCCCCEEEECCCEECCCHHHEEEEEECCCCHHCCCCHHHHHHHHHHHCCCCEEHHH
VTKKERKRNPALPFTTSTLQQEAARKLNFRAKKTMMIAQQLYEGIDLGKEGTVGLITYMR
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEE
TDSTRISNTAQEEAASFIGEQYGKEFLGSKRKPAKKNENAQDAHEAIRPTSVLRKPSDLK
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHCCCHHHH
AVLGRDQLRLYKLIWERFVASQMAPATLDTMSVDLENNGLTFRANGSKVKFSGFMKVYVE
HHHCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCEEEEECCCEEEEEEEEEEEEE
GKDDQMEEKNKMLPDLAEGDTVLSKDIEPEQHFTQPPPRYTEARLVKTLEELGIGRPSTY
CCCHHHHHHHCCCCCCCCCCCCHHCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCC
APTLDTIQKRGYVALDNKRFIPTELGEIVLDLIMEFFPEIINVEFTAKMEKELDDVEDGN
CCCHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEHHHHHHHCCCCCCC
IQWVQIIDSFYKDFEKRVEKAEAEMQEVEIEPEYAGVDCEACGHPMVYKMGRYGKFMACS
CHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHCCCCHHHHHCCCCCEEEEC
NFPDCRNTKPIVKEIGVKCPSCKTGNIVERKSKKRRIFYGCDRYPECEFVSWDKPLERKC
CCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHCCCCEEEECCCCCCCCCEECCCCCHHHCC
PKCEDMLVEKKLKKGVQVQCVNCDYKEEQQK
CCHHHHHHHHHHHCCCEEEEECCCCHHHCCC
>Mature Secondary Structure 
SEYLVIVESPAKAKTIERYLGKKYKVKASMGHVRDLPKSQMGVDIEQNFEPKYITIRGK
CCEEEEEECCCHHHHHHHHCCCCEEEEHHHHHHHCCCHHHHCCCHHCCCCCCEEEEECC
GPVLKELKTAAKKAKKVYLAADPDREGEAIAWHLAHSLDLDLNSDCRVVFNEITKDAIKE
CCHHHHHHHHHHHHHEEEEEECCCCCCCEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHH
SFKHPRMINMDLVDAQQARRILDRLVGYKISPILWKKVKKGLSAGRVQSVALRLIIDREK
HHCCCCEEEEHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCH
EINDFIPEEYWTIEGSFLKGKETFEASFFGINGEKHQLKSEDDVKQILSRIKGNKFEVKK
HHHHCCCCCEEEECCCEECCCHHHEEEEEECCCCHHCCCCHHHHHHHHHHHCCCCEEHHH
VTKKERKRNPALPFTTSTLQQEAARKLNFRAKKTMMIAQQLYEGIDLGKEGTVGLITYMR
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEE
TDSTRISNTAQEEAASFIGEQYGKEFLGSKRKPAKKNENAQDAHEAIRPTSVLRKPSDLK
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHCCCHHHH
AVLGRDQLRLYKLIWERFVASQMAPATLDTMSVDLENNGLTFRANGSKVKFSGFMKVYVE
HHHCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCEEEEECCCEEEEEEEEEEEEE
GKDDQMEEKNKMLPDLAEGDTVLSKDIEPEQHFTQPPPRYTEARLVKTLEELGIGRPSTY
CCCHHHHHHHCCCCCCCCCCCCHHCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCC
APTLDTIQKRGYVALDNKRFIPTELGEIVLDLIMEFFPEIINVEFTAKMEKELDDVEDGN
CCCHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEHHHHHHHCCCCCCC
IQWVQIIDSFYKDFEKRVEKAEAEMQEVEIEPEYAGVDCEACGHPMVYKMGRYGKFMACS
CHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHCCCCHHHHHCCCCCEEEEC
NFPDCRNTKPIVKEIGVKCPSCKTGNIVERKSKKRRIFYGCDRYPECEFVSWDKPLERKC
CCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHCCCCEEEECCCCCCCCCEECCCCCHHHCC
PKCEDMLVEKKLKKGVQVQCVNCDYKEEQQK
CCHHHHHHHHHHHCCCEEEEECCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]