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The map label for this gene is yloN

Identifier: 52785555

GI number: 52785555

Start: 1754945

End: 1756030

Strand: Direct

Name: yloN

Synonym: BLi01796

Alternate gene names: 52785555

Gene position: 1754945-1756030 (Clockwise)

Preceding gene: 52785554

Following gene: 52785556

Centisome position: 41.56

GC content: 47.42

Gene sequence:

>1086_bases
ATGGAATCGAAACAAAAAGTAAGAAAAGAATTGCGGACAGAAAGACCGTCCATCTATTCTTTTGAAATAGAAGAACTGAA
AAGCTGGCTATCGGAAAACGGCGAGAAGCCATTCCGCGCCGCGCAAATATTCGAATGGCTATACGAAAAGAGAGTCACGT
CATTTGAAAAGATGACAAACCTTTCAAAAGAGCTGCGGACAAAGCTGAATGAGCATTTCGTATTAACGACGCTGAAAACG
GCCGTCAAACAGACTTCCCAAGATGGAACAATGAAGTTTTTATTCGAGCTTCATGACGGCTACACGATCGAGACCGTATT
AATGCGGCACGAATATGGCAATTCTGTTTGTGTAACGACACAGGTGGGCTGCCGGATCGGCTGTACGTTCTGCGCATCGA
CCCTCGGCGGCTTAAAGCGGAACCTGGAAGCCGGAGAGATCGTCGCACAGGTTGTCAAAGTGCAGCAGGCGCTTGATGAA
ACAGATGAACGCGTCAGCTCAATCGTCATTATGGGAATCGGCGAGCCGTTCGATAATTTTCAGGAAATGCTTGCGTTCTT
GAAAATCGTCAACCATGATAAAGGACTGAATATCGGAGCGCGCCATATCACCGTATCAACGAGCGGGATCATTCCGAAAA
TCTACGAGTTTGCAGACGAAAAGCTGCAGATCAACTTTGCGATTTCTCTGCACGCGCCGAACACTGAGATCAGAAGCCGG
CTGATGCCGATCAACAGAGCCTACAAGCTGCCCGATCTGATGAAAGCGGTCGATTACTACATTAAGAAAACCGGCAGAAG
GGTTACGTTTGAATACGGGCTTTTCGGCGGTGTCAACGATCAGGTTGAACACGCGGAAGAGCTGGCTGAATTGCTAAAAG
GAATCAAATGCCACGTCAACTTGATTCCGGTCAACTATGTACCTGAACGAGACTATGTCAGAACACCGAAAGAACAGATT
TTCGCCTTTGAAAAAACATTAAAGTCTCATGGAGTAAATGTCACGATCAGAAGAGAGCAAGGCCATGATATTGACGCTGC
CTGCGGACAGCTTCGTGCAAAGGAGCGCCAGGAAGAGACGAGGTGA

Upstream 100 bases:

>100_bases
CGGCGCCGTATGTCAAGGATGGAAGCATTCAAATCCTTCCCCACTACTTTGGAACGGACGGCTTCTTTATTTGCAGCATG
AGAAAGAAGGGATCACAATA

Downstream 100 bases:

>100_bases
ATCAAGGTTGAAAACAGCCTTAAAAACGGACAGGGGAAAAATACGCCAGCACAATGAAGACGATGCCGGCATTTTCACAG
AAAAAAACGGACTGGTATTA

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MESKQKVRKELRTERPSIYSFEIEELKSWLSENGEKPFRAAQIFEWLYEKRVTSFEKMTNLSKELRTKLNEHFVLTTLKT
AVKQTSQDGTMKFLFELHDGYTIETVLMRHEYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVAQVVKVQQALDE
TDERVSSIVIMGIGEPFDNFQEMLAFLKIVNHDKGLNIGARHITVSTSGIIPKIYEFADEKLQINFAISLHAPNTEIRSR
LMPINRAYKLPDLMKAVDYYIKKTGRRVTFEYGLFGGVNDQVEHAEELAELLKGIKCHVNLIPVNYVPERDYVRTPKEQI
FAFEKTLKSHGVNVTIRREQGHDIDAACGQLRAKERQEETR

Sequences:

>Translated_361_residues
MESKQKVRKELRTERPSIYSFEIEELKSWLSENGEKPFRAAQIFEWLYEKRVTSFEKMTNLSKELRTKLNEHFVLTTLKT
AVKQTSQDGTMKFLFELHDGYTIETVLMRHEYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVAQVVKVQQALDE
TDERVSSIVIMGIGEPFDNFQEMLAFLKIVNHDKGLNIGARHITVSTSGIIPKIYEFADEKLQINFAISLHAPNTEIRSR
LMPINRAYKLPDLMKAVDYYIKKTGRRVTFEYGLFGGVNDQVEHAEELAELLKGIKCHVNLIPVNYVPERDYVRTPKEQI
FAFEKTLKSHGVNVTIRREQGHDIDAACGQLRAKERQEETR
>Mature_361_residues
MESKQKVRKELRTERPSIYSFEIEELKSWLSENGEKPFRAAQIFEWLYEKRVTSFEKMTNLSKELRTKLNEHFVLTTLKT
AVKQTSQDGTMKFLFELHDGYTIETVLMRHEYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVAQVVKVQQALDE
TDERVSSIVIMGIGEPFDNFQEMLAFLKIVNHDKGLNIGARHITVSTSGIIPKIYEFADEKLQINFAISLHAPNTEIRSR
LMPINRAYKLPDLMKAVDYYIKKTGRRVTFEYGLFGGVNDQVEHAEELAELLKGIKCHVNLIPVNYVPERDYVRTPKEQI
FAFEKTLKSHGVNVTIRREQGHDIDAACGQLRAKERQEETR

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=335, Percent_Identity=38.8059701492537, Blast_Score=253, Evalue=1e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_BACLD (Q65JS3)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_078969.1
- RefSeq:   YP_091384.1
- ProteinModelPortal:   Q65JS3
- STRING:   Q65JS3
- EnsemblBacteria:   EBBACT00000054757
- EnsemblBacteria:   EBBACT00000058878
- GeneID:   3030957
- GeneID:   3100774
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi01796
- KEGG:   bli:BL02300
- NMPDR:   fig|279010.5.peg.1865
- eggNOG:   COG0820
- GeneTree:   EBGT00050000001327
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   PRK14455
- BioCyc:   BLIC279010-1:BLI01796-MONOMER
- BioCyc:   BLIC279010:BL02300-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 41386; Mature: 41386

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESKQKVRKELRTERPSIYSFEIEELKSWLSENGEKPFRAAQIFEWLYEKRVTSFEKMTN
CCCHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LSKELRTKLNEHFVLTTLKTAVKQTSQDGTMKFLFELHDGYTIETVLMRHEYGNSVCVTT
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEHHHHHHHCCCEEEEEE
QVGCRIGCTFCASTLGGLKRNLEAGEIVAQVVKVQQALDETDERVSSIVIMGIGEPFDNF
ECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHH
QEMLAFLKIVNHDKGLNIGARHITVSTSGIIPKIYEFADEKLQINFAISLHAPNTEIRSR
HHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHCCCEEEEEEEEEEECCCHHHHHH
LMPINRAYKLPDLMKAVDYYIKKTGRRVTFEYGLFGGVNDQVEHAEELAELLKGIKCHVN
HCCCHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEE
LIPVNYVPERDYVRTPKEQIFAFEKTLKSHGVNVTIRREQGHDIDAACGQLRAKERQEET
EEEECCCCCCCHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCC
R
C
>Mature Secondary Structure
MESKQKVRKELRTERPSIYSFEIEELKSWLSENGEKPFRAAQIFEWLYEKRVTSFEKMTN
CCCHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LSKELRTKLNEHFVLTTLKTAVKQTSQDGTMKFLFELHDGYTIETVLMRHEYGNSVCVTT
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEHHHHHHHCCCEEEEEE
QVGCRIGCTFCASTLGGLKRNLEAGEIVAQVVKVQQALDETDERVSSIVIMGIGEPFDNF
ECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHH
QEMLAFLKIVNHDKGLNIGARHITVSTSGIIPKIYEFADEKLQINFAISLHAPNTEIRSR
HHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHCCCEEEEEEEEEEECCCHHHHHH
LMPINRAYKLPDLMKAVDYYIKKTGRRVTFEYGLFGGVNDQVEHAEELAELLKGIKCHVN
HCCCHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEE
LIPVNYVPERDYVRTPKEQIFAFEKTLKSHGVNVTIRREQGHDIDAACGQLRAKERQEET
EEEECCCCCCCHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA