Click here to switch to the map view.

The map label for this gene is priA [H]

Identifier: 52785551

GI number: 52785551

Start: 1749725

End: 1752142

Strand: Direct

Name: priA [H]

Synonym: BLi01792

Alternate gene names: 52785551

Gene position: 1749725-1752142 (Clockwise)

Preceding gene: 52785550

Following gene: 52785552

Centisome position: 41.44

GC content: 49.88

Gene sequence:

>2418_bases
ATGAACTTCGCTGAAGTGATCGTCGATGTTACAACGAAAAATATCGACAGACCGTTTGATTACCGCATCCCCGACAAATT
CAAAGGTATGATTAAAAAAGGGATGCGCGTGATCGTTCCGTTCGGCGCCCGCAAAATCCAGGGATTTGTCACCAGCGTGA
AAGAATCGTCGGAGCTTGCCGGCCGTTCGGTTAAGGATATCGAGGATATCCTTGATCTGGCGCCCGTTTTGACGGAGGAG
CTGCTTGAGCTGTCAGACTGGCTGACAGAAAAAACACTTTCTTTTAAAATTACGGCTTTGCAGGCGATGCTCCCCGCGGC
TATGAAAGCAAAATACGGCAAAGAAATCAAGGTGATCGGCGATAAACCGCTTCCCGAACCGCTAAAAACCTTGTTCTCGC
AAAAAGAGACGCTTTCTTATTCCGATATAAATGACCACGGAACATTAAAGCTGATTCAGCGCCAGGTTCAAGAAGGCAAT
CTTGAAGTGGCGTATAAAGTAAAGCAGAAAACAAACAAAAAAATGCGGAGAATGATCGCCCCCCTTTGCGCGAAAGAACA
TTTAAAAGAAGCGCTTGCCGGCCTCTCTCCGCAAGCTGTCAAACAAAAAGCGATCCTTCAGTTTTTCATCGACCGCAAAG
AGGGCGGCGCTGTTCCGGCAAAGGATCTGATCGACAAAACGGGGGGCTCTTCCGCGTCCTTGAAAGCGCTCATCGACAAG
GGGCTGTTAAAGGAAAGCGAAGAGGAGGTTTACAGGGATCCTTATCAGGACAGAATGTTCAAAAAAACCGAACCGCTTCC
GCTGACTGAAGAGCAGGCGCAGGCCTACGAGGCGATAGCGGATGCAATCGAAGAAAAGCGGCACGATGTGTTTCTTCTTC
ACGGAGTGACCGGAAGCGGAAAAACGGAAGTCTATCTGCAATCGATCGAAAAGGTGCTGGAAGCCGGCAAAGAAGCGATC
GTTCTCGTTCCGGAAATTTCCCTGACGCCGCAGATGGTCAACCGCTTTAAAGGGCGCTTTGGCTCGCAGGTAGCCGTACT
GCACAGCGGGCTTTCAACAGGAGAAAAATATGATGAATGGCGGAAAATCCACCGCAAAGAAGTCCGGCTTGTCGTCGGGG
CGCGCTCGGCGATTTTCGCGCCGTTTGAAAATCTCGGCATGATTATAATTGATGAAGAGCACGAAACATCGTACAAGCAG
GAGGAGATGCCGAGATACCATGCGAAAGAAGCGGCGATTCAAAGGGCCAAATACCACCGGTGTCCGGTCGTGCTCGGCAG
TGCGACGCCGACGCTCGAATCGTTCGCGAGGGCGAAAAAAGGCGTGTACAAGCTTCTTTCTCTCAAGCACCGCGTCAATC
ACCAAGTCATGCCCGACGTGTCTCTCGTCGATATGAGAGAGGAACTGCGCAGCGGAAACCGCTCGATGTTTTCCAAAGAC
TTAATGGCCGGGCTTGAGGAAACTCTTGAAAAAGGAGAGCAGGCCGTTCTGTTTTTAAACAAAAGAGGCTATTCCTCATT
TGTCATGTGCAGGGACTGCGGATATGTCATCCAGTGTCCTCATTGTGAAATTTCATTGACGTACCACAGGTATGGACAGC
GTCTGAAGTGCCATTACTGCGGCTATGAAACCGGCATGCCGTCAAGCTGCCCCGAATGCGGAAGCGAGCATATCCGCTAC
TTTGGTACAGGCACACAGCGGGTCGAAGAAGAATTGACGAAGGTTCTGCCGGCTGCAAGGGTGATCCGCATGGATGTCGA
TACAACCTCAAGAAAGGGAGCGCATGAAAAGCTGCTCACATCCTTTGGCAGAGGCGAAGCCGATATCCTGCTTGGAACGC
AGATGATCGCCAAAGGGCTCGATTTCGAAAACGTCACGCTCGTCGGTGTCTTGAGCGCGGATACGATGCTTCACATCCCC
GATTTCAGAGCCGCTGAAAAAACGTTCCAGCTGATGACGCAGGTGAGCGGAAGAGCGGGGCGCCACGAAAAGCCGGGCAA
GGTGATCATTCAGACGTACACACCGTCCCATTACAGCATTCAGCTCACAAAAACGCATGATTATGAAACATTCTTCCGGC
AGGAGATGCTGCACAGAAGGGAACAGGCCTATCCTCCATATTATTATTTGGCGCTGGTCAGCGTATCGCACGAGGAAGTG
ACGAAGGCGGCACTCGTGACTGAAAAAATCGCCACCTATTTAAAAAAGAACATTTCAAGCGAGGCGAAGATTCTCGGCCC
TGTCGCATCTCCGATCGCCAGGATCAAAGATAGATATCGCTATCAATGCGTGATAAAATACAAACAGGAAAACCAGCTTA
CATCGTTGCTTAAAAACATTATGGATCATTACAAACGAGACATTGAACAAAAGCAATTGGCGATTTCGATCGACATGAAT
CCATATATGATGATGTAA

Upstream 100 bases:

>100_bases
ACTGCAAAAAGACGGCGCATCCTCTAATGTCAAAGCGGGAGGTTGCCGCCGCAGTCTTGAAGGAAGCCGCTGAGCTTGCC
GGACGGAGCGGGACGTCTTC

Downstream 100 bases:

>100_bases
AAATAACAGTGTGATGGAGGGCTTTTAGATTGGCAGTAAAACCGATCGTAACGTATCCGGCGGAGGTTCTTGAGACCCCT
GCCGAACCCGTTGCGTCTTT

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 805; Mature: 805

Protein sequence:

>805_residues
MNFAEVIVDVTTKNIDRPFDYRIPDKFKGMIKKGMRVIVPFGARKIQGFVTSVKESSELAGRSVKDIEDILDLAPVLTEE
LLELSDWLTEKTLSFKITALQAMLPAAMKAKYGKEIKVIGDKPLPEPLKTLFSQKETLSYSDINDHGTLKLIQRQVQEGN
LEVAYKVKQKTNKKMRRMIAPLCAKEHLKEALAGLSPQAVKQKAILQFFIDRKEGGAVPAKDLIDKTGGSSASLKALIDK
GLLKESEEEVYRDPYQDRMFKKTEPLPLTEEQAQAYEAIADAIEEKRHDVFLLHGVTGSGKTEVYLQSIEKVLEAGKEAI
VLVPEISLTPQMVNRFKGRFGSQVAVLHSGLSTGEKYDEWRKIHRKEVRLVVGARSAIFAPFENLGMIIIDEEHETSYKQ
EEMPRYHAKEAAIQRAKYHRCPVVLGSATPTLESFARAKKGVYKLLSLKHRVNHQVMPDVSLVDMREELRSGNRSMFSKD
LMAGLEETLEKGEQAVLFLNKRGYSSFVMCRDCGYVIQCPHCEISLTYHRYGQRLKCHYCGYETGMPSSCPECGSEHIRY
FGTGTQRVEEELTKVLPAARVIRMDVDTTSRKGAHEKLLTSFGRGEADILLGTQMIAKGLDFENVTLVGVLSADTMLHIP
DFRAAEKTFQLMTQVSGRAGRHEKPGKVIIQTYTPSHYSIQLTKTHDYETFFRQEMLHRREQAYPPYYYLALVSVSHEEV
TKAALVTEKIATYLKKNISSEAKILGPVASPIARIKDRYRYQCVIKYKQENQLTSLLKNIMDHYKRDIEQKQLAISIDMN
PYMMM

Sequences:

>Translated_805_residues
MNFAEVIVDVTTKNIDRPFDYRIPDKFKGMIKKGMRVIVPFGARKIQGFVTSVKESSELAGRSVKDIEDILDLAPVLTEE
LLELSDWLTEKTLSFKITALQAMLPAAMKAKYGKEIKVIGDKPLPEPLKTLFSQKETLSYSDINDHGTLKLIQRQVQEGN
LEVAYKVKQKTNKKMRRMIAPLCAKEHLKEALAGLSPQAVKQKAILQFFIDRKEGGAVPAKDLIDKTGGSSASLKALIDK
GLLKESEEEVYRDPYQDRMFKKTEPLPLTEEQAQAYEAIADAIEEKRHDVFLLHGVTGSGKTEVYLQSIEKVLEAGKEAI
VLVPEISLTPQMVNRFKGRFGSQVAVLHSGLSTGEKYDEWRKIHRKEVRLVVGARSAIFAPFENLGMIIIDEEHETSYKQ
EEMPRYHAKEAAIQRAKYHRCPVVLGSATPTLESFARAKKGVYKLLSLKHRVNHQVMPDVSLVDMREELRSGNRSMFSKD
LMAGLEETLEKGEQAVLFLNKRGYSSFVMCRDCGYVIQCPHCEISLTYHRYGQRLKCHYCGYETGMPSSCPECGSEHIRY
FGTGTQRVEEELTKVLPAARVIRMDVDTTSRKGAHEKLLTSFGRGEADILLGTQMIAKGLDFENVTLVGVLSADTMLHIP
DFRAAEKTFQLMTQVSGRAGRHEKPGKVIIQTYTPSHYSIQLTKTHDYETFFRQEMLHRREQAYPPYYYLALVSVSHEEV
TKAALVTEKIATYLKKNISSEAKILGPVASPIARIKDRYRYQCVIKYKQENQLTSLLKNIMDHYKRDIEQKQLAISIDMN
PYMMM
>Mature_805_residues
MNFAEVIVDVTTKNIDRPFDYRIPDKFKGMIKKGMRVIVPFGARKIQGFVTSVKESSELAGRSVKDIEDILDLAPVLTEE
LLELSDWLTEKTLSFKITALQAMLPAAMKAKYGKEIKVIGDKPLPEPLKTLFSQKETLSYSDINDHGTLKLIQRQVQEGN
LEVAYKVKQKTNKKMRRMIAPLCAKEHLKEALAGLSPQAVKQKAILQFFIDRKEGGAVPAKDLIDKTGGSSASLKALIDK
GLLKESEEEVYRDPYQDRMFKKTEPLPLTEEQAQAYEAIADAIEEKRHDVFLLHGVTGSGKTEVYLQSIEKVLEAGKEAI
VLVPEISLTPQMVNRFKGRFGSQVAVLHSGLSTGEKYDEWRKIHRKEVRLVVGARSAIFAPFENLGMIIIDEEHETSYKQ
EEMPRYHAKEAAIQRAKYHRCPVVLGSATPTLESFARAKKGVYKLLSLKHRVNHQVMPDVSLVDMREELRSGNRSMFSKD
LMAGLEETLEKGEQAVLFLNKRGYSSFVMCRDCGYVIQCPHCEISLTYHRYGQRLKCHYCGYETGMPSSCPECGSEHIRY
FGTGTQRVEEELTKVLPAARVIRMDVDTTSRKGAHEKLLTSFGRGEADILLGTQMIAKGLDFENVTLVGVLSADTMLHIP
DFRAAEKTFQLMTQVSGRAGRHEKPGKVIIQTYTPSHYSIQLTKTHDYETFFRQEMLHRREQAYPPYYYLALVSVSHEEV
TKAALVTEKIATYLKKNISSEAKILGPVASPIARIKDRYRYQCVIKYKQENQLTSLLKNIMDHYKRDIEQKQLAISIDMN
PYMMM

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=575, Percent_Identity=37.9130434782609, Blast_Score=418, Evalue=1e-118,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 91317; Mature: 91317

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFAEVIVDVTTKNIDRPFDYRIPDKFKGMIKKGMRVIVPFGARKIQGFVTSVKESSELA
CCHHHEEEEHHHCCCCCCCCCCCCHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHC
GRSVKDIEDILDLAPVLTEELLELSDWLTEKTLSFKITALQAMLPAAMKAKYGKEIKVIG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
DKPLPEPLKTLFSQKETLSYSDINDHGTLKLIQRQVQEGNLEVAYKVKQKTNKKMRRMIA
CCCCCHHHHHHHCHHHCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHH
PLCAKEHLKEALAGLSPQAVKQKAILQFFIDRKEGGAVPAKDLIDKTGGSSASLKALIDK
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCHHHHHHHHH
GLLKESEEEVYRDPYQDRMFKKTEPLPLTEEQAQAYEAIADAIEEKRHDVFLLHGVTGSG
HHHCCCHHHHHHCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
KTEVYLQSIEKVLEAGKEAIVLVPEISLTPQMVNRFKGRFGSQVAVLHSGLSTGEKYDEW
CHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCHHHHHCCCCCCHHHHHH
RKIHRKEVRLVVGARSAIFAPFENLGMIIIDEEHETSYKQEEMPRYHAKEAAIQRAKYHR
HHHHHHHHHEEEECCHHHHCCHHHCCEEEEECCCCCCCHHHHCCHHHHHHHHHHHHHHCC
CPVVLGSATPTLESFARAKKGVYKLLSLKHRVNHQVMPDVSLVDMREELRSGNRSMFSKD
CCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHH
LMAGLEETLEKGEQAVLFLNKRGYSSFVMCRDCGYVIQCPHCEISLTYHRYGQRLKCHYC
HHHHHHHHHHCCCEEEEEEECCCCCCEEEEECCCCEEECCCCEEEEEHHHCCCCEEEEEC
GYETGMPSSCPECGSEHIRYFGTGTQRVEEELTKVLPAARVIRMDVDTTSRKGAHEKLLT
CCCCCCCCCCHHHHHHHEEEECCCHHHHHHHHHHHCCHHHEEEEECCCCCCCCHHHHHHH
SFGRGEADILLGTQMIAKGLDFENVTLVGVLSADTMLHIPDFRAAEKTFQLMTQVSGRAG
HCCCCCHHEEECHHHHHCCCCCCCEEEEEEECCCCEEECCCCHHHHHHHHHHHHHHCCCC
RHEKPGKVIIQTYTPSHYSIQLTKTHDYETFFRQEMLHRREQAYPPYYYLALVSVSHEEV
CCCCCCEEEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCHHHH
TKAALVTEKIATYLKKNISSEAKILGPVASPIARIKDRYRYQCVIKYKQENQLTSLLKNI
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEECHHHHHHHHHHHH
MDHYKRDIEQKQLAISIDMNPYMMM
HHHHHHCCCCCEEEEEECCCCEECC
>Mature Secondary Structure
MNFAEVIVDVTTKNIDRPFDYRIPDKFKGMIKKGMRVIVPFGARKIQGFVTSVKESSELA
CCHHHEEEEHHHCCCCCCCCCCCCHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHC
GRSVKDIEDILDLAPVLTEELLELSDWLTEKTLSFKITALQAMLPAAMKAKYGKEIKVIG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
DKPLPEPLKTLFSQKETLSYSDINDHGTLKLIQRQVQEGNLEVAYKVKQKTNKKMRRMIA
CCCCCHHHHHHHCHHHCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHH
PLCAKEHLKEALAGLSPQAVKQKAILQFFIDRKEGGAVPAKDLIDKTGGSSASLKALIDK
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCHHHHHHHHH
GLLKESEEEVYRDPYQDRMFKKTEPLPLTEEQAQAYEAIADAIEEKRHDVFLLHGVTGSG
HHHCCCHHHHHHCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
KTEVYLQSIEKVLEAGKEAIVLVPEISLTPQMVNRFKGRFGSQVAVLHSGLSTGEKYDEW
CHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCHHHHHCCCCCCHHHHHH
RKIHRKEVRLVVGARSAIFAPFENLGMIIIDEEHETSYKQEEMPRYHAKEAAIQRAKYHR
HHHHHHHHHEEEECCHHHHCCHHHCCEEEEECCCCCCCHHHHCCHHHHHHHHHHHHHHCC
CPVVLGSATPTLESFARAKKGVYKLLSLKHRVNHQVMPDVSLVDMREELRSGNRSMFSKD
CCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHH
LMAGLEETLEKGEQAVLFLNKRGYSSFVMCRDCGYVIQCPHCEISLTYHRYGQRLKCHYC
HHHHHHHHHHCCCEEEEEEECCCCCCEEEEECCCCEEECCCCEEEEEHHHCCCCEEEEEC
GYETGMPSSCPECGSEHIRYFGTGTQRVEEELTKVLPAARVIRMDVDTTSRKGAHEKLLT
CCCCCCCCCCHHHHHHHEEEECCCHHHHHHHHHHHCCHHHEEEEECCCCCCCCHHHHHHH
SFGRGEADILLGTQMIAKGLDFENVTLVGVLSADTMLHIPDFRAAEKTFQLMTQVSGRAG
HCCCCCHHEEECHHHHHCCCCCCCEEEEEEECCCCEEECCCCHHHHHHHHHHHHHHCCCC
RHEKPGKVIIQTYTPSHYSIQLTKTHDYETFFRQEMLHRREQAYPPYYYLALVSVSHEEV
CCCCCCEEEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCHHHH
TKAALVTEKIATYLKKNISSEAKILGPVASPIARIKDRYRYQCVIKYKQENQLTSLLKNI
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEECHHHHHHHHHHHH
MDHYKRDIEQKQLAISIDMNPYMMM
HHHHHHCCCCCEEEEEECCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]