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The map label for this gene is def [H]

Identifier: 52785552

GI number: 52785552

Start: 1752172

End: 1752654

Strand: Direct

Name: def [H]

Synonym: BLi01793

Alternate gene names: 52785552

Gene position: 1752172-1752654 (Clockwise)

Preceding gene: 52785551

Following gene: 52785553

Centisome position: 41.49

GC content: 49.69

Gene sequence:

>483_bases
TTGGCAGTAAAACCGATCGTAACGTATCCGGCGGAGGTTCTTGAGACCCCTGCCGAACCCGTTGCGTCTTTTGATAAATC
ATTAAAGAAACTACTCGACAATATGTATGATACGATGCTTGAGCTGGACGGAGTCGGACTGGCCGCTCCGCAAATCGGCG
TTTCAAAAAGAGCGGCAGTCGTCGATATCGGCGATGACACGGGAAGAATTGAACTGGTCAACCCGGAGGTCATCGAAGAG
AGCGGTGAACAAACGGGTCCTGAAGGCTGTTTGAGCTTTCCTGATCTATACGGTGAAGTGAAGCGTTCCGATTATGTAAA
GGTCAAGGCGTTCGACCGCCGCGGGAAACCGTTTACGATTGAAGCTGAGGGCTTTTTGGCGCGTGCGCTGCTGCACGAAA
TCGACCATTTGGACGGTATTTTATTCACATCAAAAGTAACGCGCTATTATGATGCGGAAGAACTTGAGGAAATGGAAGGA
TGA

Upstream 100 bases:

>100_bases
ATTACAAACGAGACATTGAACAAAAGCAATTGGCGATTTCGATCGACATGAATCCATATATGATGATGTAAAAATAACAG
TGTGATGGAGGGCTTTTAGA

Downstream 100 bases:

>100_bases
TTGGATGACAAGGATCGTTTTTATGGGAACGCCGGATTTTTCCGTCCCGGTGCTCGGCACATTGATTGATGACGGCTATG
AGGTAGTGGGTGTCGTCACC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MAVKPIVTYPAEVLETPAEPVASFDKSLKKLLDNMYDTMLELDGVGLAAPQIGVSKRAAVVDIGDDTGRIELVNPEVIEE
SGEQTGPEGCLSFPDLYGEVKRSDYVKVKAFDRRGKPFTIEAEGFLARALLHEIDHLDGILFTSKVTRYYDAEELEEMEG

Sequences:

>Translated_160_residues
MAVKPIVTYPAEVLETPAEPVASFDKSLKKLLDNMYDTMLELDGVGLAAPQIGVSKRAAVVDIGDDTGRIELVNPEVIEE
SGEQTGPEGCLSFPDLYGEVKRSDYVKVKAFDRRGKPFTIEAEGFLARALLHEIDHLDGILFTSKVTRYYDAEELEEMEG
>Mature_159_residues
AVKPIVTYPAEVLETPAEPVASFDKSLKKLLDNMYDTMLELDGVGLAAPQIGVSKRAAVVDIGDDTGRIELVNPEVIEES
GEQTGPEGCLSFPDLYGEVKRSDYVKVKAFDRRGKPFTIEAEGFLARALLHEIDHLDGILFTSKVTRYYDAEELEEMEG

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=143, Percent_Identity=41.958041958042, Blast_Score=125, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24645728, Length=162, Percent_Identity=30.2469135802469, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24645726, Length=153, Percent_Identity=30.718954248366, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 17653; Mature: 17522

Theoretical pI: Translated: 4.20; Mature: 4.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVKPIVTYPAEVLETPAEPVASFDKSLKKLLDNMYDTMLELDGVGLAAPQIGVSKRAAV
CCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEE
VDIGDDTGRIELVNPEVIEESGEQTGPEGCLSFPDLYGEVKRSDYVKVKAFDRRGKPFTI
EEECCCCCEEEEECHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCEEE
EAEGFLARALLHEIDHLDGILFTSKVTRYYDAEELEEMEG
EHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure 
AVKPIVTYPAEVLETPAEPVASFDKSLKKLLDNMYDTMLELDGVGLAAPQIGVSKRAAV
CCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEE
VDIGDDTGRIELVNPEVIEESGEQTGPEGCLSFPDLYGEVKRSDYVKVKAFDRRGKPFTI
EEECCCCCEEEEECHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCEEE
EAEGFLARALLHEIDHLDGILFTSKVTRYYDAEELEEMEG
EHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9086272; 9534248; 9384377 [H]